Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0.11 0.11 0.16 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.06 0.07 0.05 0.07 0 0.05 0 0 0.14 0.26 0.23 0.08 0.1 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.08 0 0 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0.06 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.33 0.37 0.4 0.54 0.44 0.41 0 0.09 0.05 0.07 0.08 0.07
Mitochondria 0.92 0.92 0.85 0.89 0.87 0.89 0.93 0.88 0.94 0.94 0.95 0.95 0.46 0.28 0.38 0.09 0.08 0.06 0.86 0.71 0.77 0.7 0.64 0.59
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.08 0.06 0 0.13 0.06 0.07 0.06 0.09 0.06 0.1 0.08 0.21 0.27 0.15 0.17 0.23 0.28 0 0 0 0.08 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 2 2 3 6 4 6 0 3 7 2 9 22
Bud 4 0 2 5 5 5 17 10 7 13 13 10 0 0 1 4 2 1 0 1 3 5 21 20
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 8
Bud Site 0 0 0 0 0 3 8 5 6 5 4 2 0 0 0 1 1 0 – – – – – –
Cell Periphery 3 1 18 3 3 2 3 4 1 3 4 2 13 11 13 17 17 18 0 0 1 0 0 2
Cytoplasm 5 0 11 16 15 25 28 33 17 25 15 19 35 116 83 13 15 6 1 0 0 8 8 9
Endoplasmic Reticulum 2 1 1 2 1 2 4 2 3 5 3 11 7 3 6 3 6 2 0 0 0 1 0 6
Endosome 6 0 20 2 10 3 3 16 2 2 1 1 19 28 23 4 4 3 6 4 9 11 15 21
Golgi 0 2 4 4 7 1 1 1 1 0 0 0 84 163 145 87 66 48 6 6 13 20 33 31
Mitochondria 168 48 215 183 226 332 546 420 375 453 329 437 117 123 136 14 12 7 157 51 203 211 280 272
Nucleus 0 0 1 0 0 7 7 8 5 2 7 2 1 3 1 1 2 2 0 0 0 1 3 2
Nuclear Periphery 3 0 9 4 8 29 21 8 11 8 10 13 2 2 1 4 3 4 1 0 0 1 3 3
Nucleolus 5 1 4 2 3 13 24 25 7 11 9 7 1 0 1 5 3 5 0 0 0 0 1 1
Peroxisomes 1 0 26 3 5 5 0 8 1 4 2 4 4 13 8 1 6 3 2 1 21 3 7 6
SpindlePole 4 1 6 1 3 2 12 8 10 14 7 9 2 5 11 0 2 2 2 0 0 5 9 8
Vac/Vac Membrane 26 4 16 3 33 22 41 27 34 31 34 35 54 121 53 27 34 32 2 1 0 24 36 36
Unique Cell Count 182 52 253 206 259 373 585 478 397 481 348 461 252 442 359 160 151 116 183 73 266 304 442 459
Labelled Cell Count 227 58 334 228 319 451 715 575 480 577 438 552 341 590 485 187 177 139 183 73 266 304 442 459


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 8.7 7.7 7.4 6.4 5.1 4.6 4.5 4.5 4.3 4.0 4.0 8.9 8.5 8.1 11.2 10.6 11.0 8.6 9.0 9.2
Std Deviation (1e-4) 2.0 3.1 2.5 2.9 2.1 1.9 1.6 1.4 1.5 1.4 1.4 1.3 2.5 2.5 2.3 2.2 2.2 2.4 2.5 2.5 2.7
Intensity Change (Log2) – – – -0.05 -0.26 -0.59 -0.72 -0.76 -0.76 -0.82 -0.92 -0.94 0.22 0.15 0.09 0.55 0.46 0.52 0.17 0.23 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.9 -3.4 -4.6 0 0 0 0 0 0 -0.9 -2.9 -1.9 1.2 1.4 2.5
Cytoplasm 1.5 0.7 1.2 0.3 1.4 0 0.5 0 -0.1 3.7 7.2 6.3 1.6 2.2 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.5 -1.9 -4.6 0 0 0 0 0 0 -0.2 -0.8 -0.7 -2.3 -2.2 -2.0
Golgi 0 0.9 0 0 0 0 0 0 0 9.4 10.5 11.0 12.6 10.8 10.2
Mitochondria 1.2 0.7 1.5 0 0 0 0 0 0 -9.1 -14.5 -11.6 -15.2 -15.1 -14.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.0 -0.3 2.2 0 0 0 0 0 0 -2.1 0 0 -0.6 0 0
Nucleolus 0 0 1.4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -3.9 -4.0 -5.1 0 0 0 0 0 0 -4.1 -4.0 -4.3 -3.9 -2.3 -2.5
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.5 0 0 0
Vacuole -2.6 2.5 -0.2 0.4 -0.4 1.0 0.1 1.5 0.6 4.9 6.7 3.3 3.4 4.8 5.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7238 1.9764 1.7081 0.9181 0.9861 1.779 1.5383 0.947 1.2111 0.9184 1.8297 0.9264 0.563 1.3955 1.1867 1.3967 0.7423 0.9347
Actin 0.0509 0.0253 0.0219 0.0467 0.0245 0.0059 0.0901 0.0088 0.0487 0.0397 0.0061 0.013 0.0573 0.0181 0.0106 0.0446 0.0199 0.0139
Bud 0.003 0.0067 0.008 0.0068 0.0033 0.0015 0.0025 0.0064 0.0056 0.0041 0.0052 0.0017 0.0032 0.0231 0.0102 0.0016 0.0438 0.0082
Bud Neck 0.0047 0.0025 0.0055 0.0013 0.0066 0.0061 0.0085 0.0009 0.0015 0.003 0.029 0.0068 0.0061 0.0016 0.0058 0.0007 0.0006 0.0052
Bud Periphery 0.0079 0.0126 0.0222 0.0285 0.0043 0.0041 0.0028 0.0078 0.0169 0.0068 0.0159 0.0054 0.0084 0.0339 0.0217 0.0041 0.059 0.0196
Bud Site 0.0238 0.0803 0.0368 0.0053 0.0063 0.002 0.022 0.033 0.0085 0.0099 0.0264 0.0011 0.016 0.0648 0.0284 0.0024 0.0039 0.0024
Cell Periphery 0.008 0.0045 0.0031 0.0025 0.0008 0.0019 0.0009 0.0027 0.0016 0.0016 0.0032 0.001 0.0012 0.0023 0.0017 0.0014 0.0012 0.0014
Cytoplasm 0.0684 0.1029 0.1107 0.1538 0.1354 0.1096 0.0395 0.0711 0.0694 0.0752 0.0087 0.0847 0.0587 0.081 0.078 0.0399 0.0323 0.0605
Cytoplasmic Foci 0.076 0.0849 0.0466 0.0849 0.0566 0.0708 0.1001 0.0794 0.0659 0.0752 0.065 0.0798 0.0932 0.0478 0.0586 0.0498 0.075 0.051
Eisosomes 0.0018 0.0009 0.001 0.0007 0.0002 0.0004 0.001 0.0018 0.0008 0.0008 0.0001 0.0003 0.0008 0.001 0.0004 0.0008 0.0003 0.0003
Endoplasmic Reticulum 0.0124 0.0074 0.0109 0.004 0.0016 0.0034 0.006 0.0042 0.0053 0.0056 0.0004 0.0038 0.0046 0.0031 0.0043 0.0029 0.001 0.0064
Endosome 0.0803 0.0819 0.0665 0.1083 0.0616 0.1091 0.1257 0.0888 0.0834 0.1224 0.09 0.1162 0.1273 0.0756 0.0882 0.0553 0.0827 0.1015
Golgi 0.0362 0.0518 0.0276 0.0192 0.0154 0.0658 0.071 0.0644 0.0499 0.0908 0.0406 0.0644 0.0651 0.0434 0.0418 0.0433 0.0258 0.0462
Lipid Particles 0.0437 0.0113 0.0083 0.0095 0.0023 0.026 0.0741 0.0068 0.0195 0.015 0.0063 0.0224 0.0333 0.0096 0.0155 0.0225 0.0028 0.0106
Mitochondria 0.3288 0.2778 0.4008 0.2753 0.4904 0.4225 0.2358 0.4019 0.4352 0.4343 0.5583 0.4036 0.3386 0.4188 0.452 0.4084 0.4818 0.5188
None 0.1576 0.1695 0.1784 0.1753 0.1569 0.1403 0.103 0.1806 0.1326 0.0624 0.0998 0.1379 0.1444 0.1185 0.1364 0.2548 0.1211 0.0926
Nuclear Periphery 0.0086 0.0014 0.0068 0.0017 0.0005 0.0006 0.0127 0.0012 0.0027 0.0022 0.0004 0.0031 0.0034 0.001 0.0013 0.0006 0.0003 0.0094
Nucleolus 0.0011 0.0017 0.0018 0.0023 0.0006 0.0004 0.0012 0.0008 0.0007 0.0009 0.002 0.0003 0.0009 0.0036 0.0011 0.0005 0.0017 0.0004
Nucleus 0.0151 0.0025 0.0034 0.0027 0.001 0.001 0.0122 0.0013 0.0018 0.0019 0.0023 0.0008 0.0015 0.0048 0.0016 0.0006 0.0009 0.0014
Peroxisomes 0.0311 0.0337 0.0079 0.0119 0.0172 0.0133 0.0563 0.0218 0.0354 0.0285 0.0211 0.0273 0.0215 0.023 0.0209 0.0597 0.0361 0.034
Punctate Nuclear 0.0031 0.0018 0.0036 0.0058 0.0006 0.0008 0.0091 0.0009 0.0012 0.0011 0.0024 0.0013 0.002 0.0018 0.0012 0.0009 0.0016 0.0011
Vacuole 0.0289 0.0338 0.0173 0.0444 0.009 0.0102 0.0187 0.0103 0.0091 0.0126 0.0146 0.0185 0.0084 0.0171 0.0149 0.0029 0.0049 0.0081
Vacuole Periphery 0.0085 0.0051 0.011 0.0091 0.0049 0.0045 0.0065 0.005 0.0041 0.0061 0.0021 0.0069 0.004 0.0062 0.0053 0.0023 0.003 0.007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 83.5912 67.4445 87.5818 100.0554 56.6909 71.9772 96.0903 93.6205 93.3701 83.1394
Translational Efficiency 1.5631 1.8167 1.5176 1.3786 1.8423 2.049 1.5428 1.4626 1.6286 1.45

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
Localization
Cell Percentages mitochondrion (92%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Rpt3

Rpt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available