Standard name
Human Ortholog
Description Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.65 0.41 0.57 0.57 0.53 0.41 0.51 0.5 0.53 0.55 0.72 0.74 0.67 0.91 0.92 0.92 0.1 0.3 0.39 0.53 0.51 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.05 0.06 0 0.08 0.12 0.11 0 0 0 0 0 0 0.2 0.14 0.11 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.07 0.05 0.12 0.12 0.21 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.27 0.26 0.05 0 0 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.29 0.12 0.19 0.07 0.08 0.07
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.62 0.43 0.68 0.48 0.41 0.39 0.49 0.37 0.35 0.25 0.26 0.24 0.23 0.36 0.13 0.1 0.11 0.18 0.19 0.09 0.16 0.19 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 3 0 2 1 0 0 0 0 0 0 0 0 1 1 0 0 1
Bud 0 0 0 0 1 3 6 1 2 6 1 0 0 0 0 1 0 0 12 0 2 6 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3 4 2 3 5 3 0 0 1 2 1 3 0 0 0 0 0 0
Cytoplasm 181 169 128 247 275 263 302 235 304 247 268 116 137 102 179 181 186 1 119 110 102 213 154
Endoplasmic Reticulum 8 9 8 13 20 17 33 22 38 19 38 19 21 5 6 3 4 0 15 55 27 47 31
Endosome 0 0 0 1 1 0 2 1 1 1 2 0 1 0 0 0 1 0 8 7 2 10 19
Golgi 0 2 4 2 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 1 0 1
Mitochondria 4 0 2 3 22 34 36 54 72 99 61 0 0 0 0 0 0 0 0 2 0 3 2
Nucleus 4 4 6 2 4 11 17 15 16 18 24 0 1 0 1 2 1 4 106 13 8 15 32
Nuclear Periphery 0 1 0 0 3 3 7 0 6 7 7 0 0 0 0 0 0 4 49 53 13 32 27
Nucleolus 0 1 1 0 1 1 3 1 0 1 1 0 0 0 0 0 0 0 5 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 4
Vac/Vac Membrane 222 112 216 206 198 195 357 170 211 117 127 38 43 55 26 20 22 2 74 25 31 78 91
Unique Cell Count 359 260 316 433 482 494 731 457 606 468 490 160 186 152 197 197 203 15 403 281 194 419 385
Labelled Cell Count 419 298 366 474 525 531 770 501 656 521 533 174 203 163 214 208 217 15 403 281 194 419 385


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.5 17.5 16.4 13.9 11.4 11.1 11.3 10.7 10.7 10.1 10.3 18.8 19.0 17.9 22.1 22.9 23.0 18.7 18.6 18.5
Std Deviation (1e-4) 3.4 3.2 2.6 2.4 2.2 2.2 2.3 2.2 2.2 2.2 2.3 3.5 3.2 3.4 5.2 5.6 6.1 2.7 3.2 3.2
Intensity Change (Log2) -0.1 -0.33 -0.63 -0.66 -0.63 -0.71 -0.71 -0.79 -0.77 0.1 0.11 0.03 0.33 0.38 0.39 0.09 0.08 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -5.9 -2.1 -2.1 -3.1 -6.6 -3.5 -4.0 -3.2 -2.7 1.6 1.9 0.4 6.4 6.7 6.7
Endoplasmic Reticulum -0.7 -0.3 0.5 0 0.7 0.9 1.7 0.4 2.3 3.4 3.3 -0.1 -0.2 -1.3 -1.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.5 4.3 3.6 5.8 5.8 8.0 5.9 0 0 0 0 0 0
Nucleus 0 0 0 0.6 0.8 1.4 1.0 1.7 2.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 6.1 1.2 -0.5 -1.0 1.6 -1.5 -2.3 -5.0 -4.8 -4.0 -4.4 -1.4 -6.9 -7.7 -7.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.9968 15.4347 14.4201 12.1633 13.7466 13.8854 15.4829 19.935 17.2672 15.7234 15.6306 17.4383 14.7483 18.6205 16.1626 16.5241 15.0732 16.8745
Actin 0.0005 0.0003 0.0002 0.0003 0.0002 0.0002 0.0152 0.0003 0.0038 0.0149 0.0091 0.0002 0.0014 0.0002 0.0035 0.0005 0.0002 0.0003
Bud 0.0001 0.0001 0 0 0 0 0.0002 0 0.0002 0.0004 0.0001 0 0.0004 0 0.0002 0 0.0001 0
Bud Neck 0.0016 0.0001 0.0001 0.0002 0.0004 0.0005 0.0003 0.0002 0.0013 0.0008 0.0007 0.0007 0.0018 0.0001 0.0005 0.0004 0.0002 0.0005
Bud Periphery 0 0 0 0 0 0 0.0002 0 0.0003 0.0004 0.0001 0 0.0004 0 0.0001 0 0 0
Bud Site 0.0004 0.0001 0 0 0 0 0.0008 0 0.0016 0.0022 0.0006 0 0.0038 0 0.0074 0 0 0.0001
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0002 0.0001 0 0 0.0003 0 0.0002 0 0 0
Cytoplasm 0.2048 0.275 0.3235 0.3478 0.4266 0.1657 0.0964 0.2249 0.1987 0.3373 0.2418 0.0913 0.1092 0.233 0.2291 0.4081 0.5599 0.1434
Cytoplasmic Foci 0.0054 0.0002 0.0004 0.0004 0.0004 0.0001 0.0069 0.0002 0.0055 0.015 0.0104 0.0014 0.0075 0 0.0175 0.0003 0.0003 0.0048
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0071 0.0032 0.0051 0.0138 0.0039 0.0037 0.0157 0.006 0.0117 0.0095 0.0047 0.0087 0.0056 0.0023 0.0065 0.0049 0.0028 0.0027
Endosome 0.0031 0.0004 0.0012 0.0008 0.0007 0.0007 0.015 0.0004 0.0091 0.011 0.0252 0.003 0.0137 0.0002 0.0022 0.0005 0.0008 0.004
Golgi 0.0009 0 0 0 0 0 0.0025 0 0.0029 0.0034 0.0043 0.0001 0.0031 0 0.0017 0 0 0.001
Lipid Particles 0.0022 0 0 0 0 0 0.0035 0 0.0075 0.0084 0.0065 0.0001 0.0051 0 0.0017 0 0 0.0096
Mitochondria 0.0005 0.0001 0.0002 0.0002 0.0001 0.0002 0.0037 0.0001 0.0013 0.0014 0.002 0.0002 0.0013 0.0002 0.0005 0.0002 0.0002 0.0003
None 0.001 0.0001 0.0002 0.0003 0.0002 0.0001 0.0027 0.0001 0.0012 0.0022 0.0008 0.0003 0.0002 0.0001 0.0024 0.0003 0.0003 0.0002
Nuclear Periphery 0.3436 0.2582 0.2922 0.3195 0.2017 0.2459 0.4018 0.2844 0.3369 0.2081 0.1867 0.2866 0.3763 0.2733 0.318 0.2934 0.1433 0.2833
Nucleolus 0.0004 0.0003 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001 0.0005 0.0007 0.0003 0.0004 0.0001 0.0001 0.0003 0 0.0001 0.0004
Nucleus 0.4232 0.459 0.3725 0.3122 0.361 0.5792 0.4192 0.4802 0.4069 0.3647 0.4788 0.6013 0.4639 0.4884 0.3921 0.2883 0.2882 0.5386
Peroxisomes 0.0009 0 0 0 0 0 0.0027 0 0.0016 0.0032 0.0188 0 0.0005 0 0.0081 0 0 0.0003
Punctate Nuclear 0.0012 0.0016 0.002 0.0026 0.0027 0.0012 0.0057 0.0013 0.0023 0.013 0.0063 0.0026 0.0012 0.0006 0.0056 0.0018 0.0015 0.0082
Vacuole 0.0017 0.0007 0.001 0.0007 0.0011 0.0012 0.0044 0.0011 0.0051 0.002 0.0017 0.0018 0.0029 0.0006 0.0013 0.0005 0.0013 0.0007
Vacuole Periphery 0.0013 0.0006 0.0011 0.0009 0.0008 0.0012 0.0021 0.0006 0.0015 0.0011 0.001 0.0011 0.0015 0.0008 0.0011 0.0007 0.0009 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 91.6585 65.4105 58.3482 75.6159 70.8998 50.18 63.6739 66.0068 50.7529 82.562
Translational Efficiency 0.9539 1.0765 1.1303 0.7886 0.969 1.2549 0.9643 0.705 0.9243 0.857

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
Localization
Cell Percentages nuclear periphery (28%), cytoplasm (22%), mixed (19%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sxm1

Sxm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sxm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available