Standard name | |
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Human Ortholog | |
Description | Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud | – | 0 | 0 | 0 | 0 | 1 | 3 | 6 | 1 | 2 | 6 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 12 | 0 | 2 | 6 | 7 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 3 | 5 | 3 | 0 | 0 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 181 | 169 | 128 | 247 | 275 | 263 | 302 | 235 | 304 | 247 | 268 | 116 | 137 | 102 | 179 | 181 | 186 | 1 | 119 | 110 | 102 | 213 | 154 |
Endoplasmic Reticulum | – | 8 | 9 | 8 | 13 | 20 | 17 | 33 | 22 | 38 | 19 | 38 | 19 | 21 | 5 | 6 | 3 | 4 | 0 | 15 | 55 | 27 | 47 | 31 |
Endosome | – | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 8 | 7 | 2 | 10 | 19 |
Golgi | – | 0 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | – | 4 | 0 | 2 | 3 | 22 | 34 | 36 | 54 | 72 | 99 | 61 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 2 |
Nucleus | – | 4 | 4 | 6 | 2 | 4 | 11 | 17 | 15 | 16 | 18 | 24 | 0 | 1 | 0 | 1 | 2 | 1 | 4 | 106 | 13 | 8 | 15 | 32 |
Nuclear Periphery | – | 0 | 1 | 0 | 0 | 3 | 3 | 7 | 0 | 6 | 7 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 49 | 53 | 13 | 32 | 27 |
Nucleolus | – | 0 | 1 | 1 | 0 | 1 | 1 | 3 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 1 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 4 |
Vac/Vac Membrane | – | 222 | 112 | 216 | 206 | 198 | 195 | 357 | 170 | 211 | 117 | 127 | 38 | 43 | 55 | 26 | 20 | 22 | 2 | 74 | 25 | 31 | 78 | 91 |
Unique Cell Count | 359 | 260 | 316 | 433 | 482 | 494 | 731 | 457 | 606 | 468 | 490 | 160 | 186 | 152 | 197 | 197 | 203 | 15 | 403 | 281 | 194 | 419 | 385 | |
Labelled Cell Count | 419 | 298 | 366 | 474 | 525 | 531 | 770 | 501 | 656 | 521 | 533 | 174 | 203 | 163 | 214 | 208 | 217 | 15 | 403 | 281 | 194 | 419 | 385 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 16.5 | 17.5 | 16.4 | 13.9 | 11.4 | 11.1 | 11.3 | 10.7 | 10.7 | 10.1 | 10.3 | 18.8 | 19.0 | 17.9 | 22.1 | 22.9 | 23.0 | 18.7 | 18.6 | 18.5 |
Std Deviation (1e-4) | – | 3.4 | 3.2 | 2.6 | 2.4 | 2.2 | 2.2 | 2.3 | 2.2 | 2.2 | 2.2 | 2.3 | 3.5 | 3.2 | 3.4 | 5.2 | 5.6 | 6.1 | 2.7 | 3.2 | 3.2 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.33 | -0.63 | -0.66 | -0.63 | -0.71 | -0.71 | -0.79 | -0.77 | 0.1 | 0.11 | 0.03 | 0.33 | 0.38 | 0.39 | 0.09 | 0.08 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -5.9 | -2.1 | -2.1 | -3.1 | -6.6 | -3.5 | -4.0 | -3.2 | -2.7 | 1.6 | 1.9 | 0.4 | 6.4 | 6.7 | 6.7 |
Endoplasmic Reticulum | -0.7 | -0.3 | 0.5 | 0 | 0.7 | 0.9 | 1.7 | 0.4 | 2.3 | 3.4 | 3.3 | -0.1 | -0.2 | -1.3 | -1.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.5 | 4.3 | 3.6 | 5.8 | 5.8 | 8.0 | 5.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0.6 | 0.8 | 1.4 | 1.0 | 1.7 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 6.1 | 1.2 | -0.5 | -1.0 | 1.6 | -1.5 | -2.3 | -5.0 | -4.8 | -4.0 | -4.4 | -1.4 | -6.9 | -7.7 | -7.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.9968 | 15.4347 | 14.4201 | 12.1633 | 13.7466 | 13.8854 | 15.4829 | 19.935 | 17.2672 | 15.7234 | 15.6306 | 17.4383 | 14.7483 | 18.6205 | 16.1626 | 16.5241 | 15.0732 | 16.8745 |
Actin | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0152 | 0.0003 | 0.0038 | 0.0149 | 0.0091 | 0.0002 | 0.0014 | 0.0002 | 0.0035 | 0.0005 | 0.0002 | 0.0003 |
Bud | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Bud Neck | 0.0016 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0013 | 0.0008 | 0.0007 | 0.0007 | 0.0018 | 0.0001 | 0.0005 | 0.0004 | 0.0002 | 0.0005 |
Bud Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0004 | 0.0001 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0016 | 0.0022 | 0.0006 | 0 | 0.0038 | 0 | 0.0074 | 0 | 0 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 |
Cytoplasm | 0.2048 | 0.275 | 0.3235 | 0.3478 | 0.4266 | 0.1657 | 0.0964 | 0.2249 | 0.1987 | 0.3373 | 0.2418 | 0.0913 | 0.1092 | 0.233 | 0.2291 | 0.4081 | 0.5599 | 0.1434 |
Cytoplasmic Foci | 0.0054 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0069 | 0.0002 | 0.0055 | 0.015 | 0.0104 | 0.0014 | 0.0075 | 0 | 0.0175 | 0.0003 | 0.0003 | 0.0048 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0071 | 0.0032 | 0.0051 | 0.0138 | 0.0039 | 0.0037 | 0.0157 | 0.006 | 0.0117 | 0.0095 | 0.0047 | 0.0087 | 0.0056 | 0.0023 | 0.0065 | 0.0049 | 0.0028 | 0.0027 |
Endosome | 0.0031 | 0.0004 | 0.0012 | 0.0008 | 0.0007 | 0.0007 | 0.015 | 0.0004 | 0.0091 | 0.011 | 0.0252 | 0.003 | 0.0137 | 0.0002 | 0.0022 | 0.0005 | 0.0008 | 0.004 |
Golgi | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0.0025 | 0 | 0.0029 | 0.0034 | 0.0043 | 0.0001 | 0.0031 | 0 | 0.0017 | 0 | 0 | 0.001 |
Lipid Particles | 0.0022 | 0 | 0 | 0 | 0 | 0 | 0.0035 | 0 | 0.0075 | 0.0084 | 0.0065 | 0.0001 | 0.0051 | 0 | 0.0017 | 0 | 0 | 0.0096 |
Mitochondria | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0037 | 0.0001 | 0.0013 | 0.0014 | 0.002 | 0.0002 | 0.0013 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0003 |
None | 0.001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0027 | 0.0001 | 0.0012 | 0.0022 | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0024 | 0.0003 | 0.0003 | 0.0002 |
Nuclear Periphery | 0.3436 | 0.2582 | 0.2922 | 0.3195 | 0.2017 | 0.2459 | 0.4018 | 0.2844 | 0.3369 | 0.2081 | 0.1867 | 0.2866 | 0.3763 | 0.2733 | 0.318 | 0.2934 | 0.1433 | 0.2833 |
Nucleolus | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0004 |
Nucleus | 0.4232 | 0.459 | 0.3725 | 0.3122 | 0.361 | 0.5792 | 0.4192 | 0.4802 | 0.4069 | 0.3647 | 0.4788 | 0.6013 | 0.4639 | 0.4884 | 0.3921 | 0.2883 | 0.2882 | 0.5386 |
Peroxisomes | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0.0027 | 0 | 0.0016 | 0.0032 | 0.0188 | 0 | 0.0005 | 0 | 0.0081 | 0 | 0 | 0.0003 |
Punctate Nuclear | 0.0012 | 0.0016 | 0.002 | 0.0026 | 0.0027 | 0.0012 | 0.0057 | 0.0013 | 0.0023 | 0.013 | 0.0063 | 0.0026 | 0.0012 | 0.0006 | 0.0056 | 0.0018 | 0.0015 | 0.0082 |
Vacuole | 0.0017 | 0.0007 | 0.001 | 0.0007 | 0.0011 | 0.0012 | 0.0044 | 0.0011 | 0.0051 | 0.002 | 0.0017 | 0.0018 | 0.0029 | 0.0006 | 0.0013 | 0.0005 | 0.0013 | 0.0007 |
Vacuole Periphery | 0.0013 | 0.0006 | 0.0011 | 0.0009 | 0.0008 | 0.0012 | 0.0021 | 0.0006 | 0.0015 | 0.0011 | 0.001 | 0.0011 | 0.0015 | 0.0008 | 0.0011 | 0.0007 | 0.0009 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 91.6585 | 65.4105 | 58.3482 | 75.6159 | 70.8998 | 50.18 | 63.6739 | 66.0068 | 50.7529 | 82.562 |
Translational Efficiency | 0.9539 | 1.0765 | 1.1303 | 0.7886 | 0.969 | 1.2549 | 0.9643 | 0.705 | 0.9243 | 0.857 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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