Standard name
Human Ortholog
Description Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.95 0.91 0.85 0.8 0.79 0.75 0.74 0.74 0.71 0.98 0.98 0.99 0.92 0.95 0.94 0.92 0.95 0.94 0.94 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.14 0.11 0.25 0.19 0.33 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.08 0.09 0.12 0.12 0.13 0.1 0.14 0.07 0.15 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 1 0 1 0 4 7
Bud 1 2 0 0 0 3 1 1 8 6 9 5 0 0 0 1 0 0 5 0 1 6 12 13
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 7 10 7 9 5 9 18 12 8 0 4 1 7 18 12 0 0 0 0 0 0
Cytoplasm 643 378 610 573 658 664 725 736 515 591 388 379 722 887 947 287 289 401 612 363 579 533 843 876
Endoplasmic Reticulum 1 5 2 3 1 1 3 8 4 3 0 0 2 1 2 6 10 4 5 0 2 0 7 6
Endosome 0 0 1 0 1 1 1 0 0 1 0 0 1 0 0 0 0 0 9 1 2 3 3 4
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 5 0 6 2 0 2
Mitochondria 7 1 1 9 13 70 126 101 173 152 170 113 0 0 0 4 4 4 6 0 4 2 3 7
Nucleus 2 5 9 1 8 10 17 25 21 23 13 20 0 4 5 4 2 3 0 3 5 2 1 1
Nuclear Periphery 0 2 0 0 0 0 0 7 1 6 0 0 0 0 0 0 1 0 1 2 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 1 1 0 1 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 1 0 2 0 0 0 0 0 0 2 1 1 3 7 8
Vac/Vac Membrane 8 2 6 46 63 92 106 119 68 108 34 82 22 21 17 21 21 31 4 3 3 3 4 1
Unique Cell Count 656 386 621 605 723 778 904 926 688 800 522 535 737 902 956 311 305 427 665 381 618 567 896 944
Labelled Cell Count 663 396 634 639 755 848 989 1003 799 911 627 610 748 919 972 331 347 456 665 381 618 567 896 944


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.9 7.6 7.1 7.2 6.3 6.2 6.5 5.8 5.9 5.8 5.9 8.3 8.7 8.7 10.3 11.1 11.0 7.9 8.2 8.6
Std Deviation (1e-4) 0.9 1.4 1.2 1.6 1.4 1.3 1.4 1.3 1.1 1.0 1.4 1.0 1.6 1.5 1.4 2.3 2.3 2.2 1.7 1.5 1.7
Intensity Change (Log2) – – – -0.09 -0.08 -0.26 -0.29 -0.23 -0.39 -0.37 -0.4 -0.37 0.13 0.19 0.19 0.43 0.55 0.53 0.06 0.1 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.7 0 0 0 1.6 2.7 2.7 1.8 0 0 0 0 5.2 3.1
Cytoplasm -3.3 -5.7 -8.4 -10.5 -10.7 -12.1 -12.6 -12.1 -13.2 -0.4 0.2 1.4 -4.5 -3.0 -3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.9 7.5 9.6 8.3 13.3 11.4 15.3 11.9 0 0 0 0 0 0
Nucleus 0 -0.6 -0.3 0.6 1.6 1.9 1.8 1.3 2.5 0 -2.1 -1.9 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 5.8 6.4 7.9 7.9 8.4 7.0 8.6 5.1 9.2 2.6 2.0 1.3 5.0 5.0 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3702 4.5147 4.0312 3.5868 3.4078 3.9974 2.1338 3.6548 3.6203 2.5534 3.1923 3.3076 2.3961 3.8288 3.1324 3.0232 2.7496 2.9401
Actin 0.0008 0.0005 0.0016 0.0055 0.0025 0.0027 0.0027 0.0016 0.0009 0.0002 0.0022 0.0005 0.0134 0.0003 0.0058 0.0016 0.0002 0.0011
Bud 0.0007 0.0023 0.01 0.0011 0.0012 0.0063 0.0001 0.0002 0.0049 0.0001 0.0128 0.0032 0.001 0.0001 0.0006 0.0013 0.0001 0.0006
Bud Neck 0.0003 0.0015 0.0007 0.0008 0.0015 0.0014 0.0004 0.0002 0.0005 0.0002 0.0008 0.0013 0.0125 0.0002 0.0036 0.004 0.0004 0.001
Bud Periphery 0.0001 0.0005 0.0023 0.0006 0.0004 0.0017 0.0001 0.0001 0.0013 0 0.0088 0.0017 0.0009 0 0.0005 0.0013 0 0.0002
Bud Site 0.0005 0.0117 0.0036 0.001 0.0008 0.0002 0.0003 0.0008 0.0015 0.0001 0.0005 0.0001 0.0052 0.0005 0.0032 0.0112 0.0001 0.0001
Cell Periphery 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0002 0 0.0001 0.0004 0 0
Cytoplasm 0.71 0.8468 0.7612 0.7658 0.6891 0.7854 0.7377 0.9119 0.8384 0.8132 0.8102 0.8628 0.7605 0.9492 0.8953 0.8966 0.8676 0.9348
Cytoplasmic Foci 0.0085 0.0079 0.0088 0.0135 0.0213 0.0163 0.009 0.006 0.0082 0.0036 0.0132 0.0079 0.0197 0.0024 0.0091 0.0103 0.0152 0.0076
Eisosomes 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0033 0.0021 0.0032 0.0032 0.0045 0.0025 0.0052 0.0022 0.0035 0.0022 0.0127 0.0031 0.0062 0.0021 0.0022 0.0038 0.001 0.0028
Endosome 0.0038 0.0028 0.0037 0.0047 0.0152 0.0057 0.0069 0.0031 0.0034 0.0019 0.0129 0.0029 0.0235 0.0015 0.0038 0.0113 0.0022 0.0048
Golgi 0.0002 0.0002 0.0003 0.0007 0.0011 0.0005 0.0005 0.0003 0.0002 0.0001 0.0008 0.0001 0.0028 0.0001 0.0012 0.0009 0.0002 0.0002
Lipid Particles 0.0002 0.0001 0.0001 0.0002 0.0005 0.0001 0.0005 0.0001 0.0002 0 0.0008 0 0.0037 0.0001 0.002 0.0013 0.0009 0.0002
Mitochondria 0.0003 0.0007 0.0006 0.0007 0.0023 0.0015 0.0007 0.0002 0.0003 0.0002 0.0006 0.0002 0.0043 0.0001 0.0019 0.0029 0.0001 0.0003
None 0.2503 0.0826 0.1709 0.1646 0.2047 0.1358 0.2018 0.0642 0.1095 0.1665 0.1034 0.0966 0.1003 0.0334 0.0548 0.0185 0.0581 0.0211
Nuclear Periphery 0.0023 0.0021 0.0036 0.0041 0.0035 0.0028 0.0091 0.0013 0.0026 0.0021 0.0046 0.0016 0.0092 0.0014 0.0021 0.004 0.0018 0.0058
Nucleolus 0.0003 0.0005 0.0008 0.0003 0.0034 0.0004 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0013 0.0001 0.0003 0.0043 0.0004 0.0001
Nucleus 0.0138 0.0323 0.0195 0.0184 0.022 0.0211 0.0188 0.0054 0.014 0.006 0.0093 0.0094 0.0218 0.0062 0.0083 0.0135 0.0122 0.012
Peroxisomes 0.0001 0.0002 0.0003 0.0011 0.002 0.0031 0.0003 0.0002 0.0004 0 0.0003 0.0001 0.0023 0 0.0016 0.0006 0.0007 0.0002
Punctate Nuclear 0.0029 0.0034 0.007 0.0126 0.0222 0.0113 0.0033 0.0009 0.008 0.0025 0.0034 0.0074 0.007 0.0012 0.0025 0.0045 0.038 0.0059
Vacuole 0.0012 0.0013 0.0012 0.0008 0.0012 0.001 0.0016 0.0011 0.0016 0.0007 0.0019 0.0008 0.003 0.0008 0.0006 0.0062 0.0005 0.0009
Vacuole Periphery 0.0002 0.0002 0.0002 0.0002 0.0004 0.0002 0.0005 0.0002 0.0002 0.0002 0.0005 0.0001 0.001 0.0001 0.0002 0.0014 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.8621 26.0334 20.7489 29.5068 53.141 67.914 33.1466 22.3717 28.6795 57.0569
Translational Efficiency 1.0262 1.3795 1.0903 0.8064 0.9179 1.039 0.9679 1.0411 0.9649 0.9544

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Urh1

Urh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Urh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available