Standard name | |
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Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 4 | 3 | 6 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | – | 1 | 3 | 1 | 2 | 2 | 2 |
Bud | 2 | 2 | 8 | 11 | 3 | 4 | 7 | 8 | 15 | 16 | 14 | 10 | 0 | 1 | 0 | – | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 7 | 6 | 3 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – |
Cell Periphery | 6 | 6 | 13 | 11 | 4 | 9 | 6 | 10 | 7 | 4 | 3 | 4 | 1 | 7 | 1 | – | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 0 | 8 | 16 | 17 | 4 | 19 | 18 | 11 | 9 | 11 | 4 | 2 | 8 | 7 | – | 1 | – | 0 | 0 | 5 | 2 | 1 | 0 |
Endoplasmic Reticulum | 2 | 4 | 2 | 2 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 |
Endosome | 6 | 2 | 10 | 13 | 26 | 18 | 23 | 33 | 10 | 9 | 7 | 10 | 1 | 6 | 5 | – | 1 | – | 10 | 6 | 11 | 3 | 8 | 6 |
Golgi | 15 | 6 | 40 | 53 | 46 | 15 | 16 | 30 | 0 | 0 | 3 | 4 | 6 | 28 | 32 | – | 6 | – | 15 | 16 | 14 | 7 | 20 | 10 |
Mitochondria | 243 | 147 | 191 | 217 | 248 | 374 | 339 | 322 | 404 | 385 | 287 | 360 | 23 | 100 | 92 | – | 1 | – | 230 | 126 | 236 | 52 | 81 | 90 |
Nucleus | 2 | 0 | 2 | 0 | 1 | 3 | 5 | 6 | 2 | 3 | 1 | 3 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 16 | 11 | 12 | 3 | 9 | 38 | 34 | 30 | 33 | 33 | 27 | 41 | 2 | 1 | 2 | – | 0 | – | 2 | 0 | 0 | 1 | 0 | 2 |
Nucleolus | 18 | 4 | 3 | 7 | 17 | 34 | 24 | 29 | 31 | 30 | 27 | 27 | 1 | 2 | 1 | – | 0 | – | 2 | 0 | 0 | 2 | 1 | 0 |
Peroxisomes | 1 | 2 | 30 | 15 | 25 | 11 | 4 | 14 | 10 | 14 | 5 | 4 | 0 | 8 | 3 | – | 0 | – | 15 | 6 | 20 | 3 | 2 | 4 |
SpindlePole | 2 | 0 | 4 | 5 | 7 | 10 | 8 | 15 | 8 | 11 | 6 | 5 | 0 | 0 | 0 | – | 0 | – | 2 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 31 | 17 | 28 | 30 | 64 | 56 | 67 | 105 | 34 | 25 | 27 | 31 | 2 | 13 | 9 | – | 5 | – | 3 | 7 | 1 | 1 | 1 | 5 |
Unique Cell Count | 273 | 157 | 249 | 275 | 355 | 468 | 451 | 508 | 452 | 439 | 341 | 406 | 28 | 126 | 115 | 17 | 287 | 167 | 299 | 83 | 125 | 130 | ||
Labelled Cell Count | 350 | 205 | 354 | 389 | 467 | 579 | 556 | 624 | 572 | 545 | 421 | 503 | 39 | 175 | 156 | 18 | 287 | 167 | 299 | 83 | 125 | 130 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 7.4 | 9.2 | 9.5 | 9.8 | 8.5 | 8.3 | 9.1 | 7.4 | 7.4 | 7.5 | 7.3 | 8.6 | 9.6 | 8.5 | – | 7.8 | – | 10.5 | 11.8 | 10.4 |
Std Deviation (1e-4) | 1.6 | 1.1 | 2.0 | 2.1 | 1.8 | 1.7 | 1.8 | 2.1 | 2.0 | 1.6 | 2.2 | 1.6 | 1.2 | 1.7 | 1.6 | – | 1.0 | – | 1.9 | 11.4 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.09 | -0.1 | -0.15 | -0.01 | -0.32 | -0.3 | -0.28 | -0.33 | -0.09 | 0.06 | -0.12 | – | -0.23 | – | 0.19 | 0.36 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Bud | 0.5 | 0 | 0 | -1.5 | -1.5 | 0.1 | 0.3 | 0.6 | -0.6 | 0 | 0 | 0 | – | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Cell Periphery | -0.7 | -3.0 | -2.4 | -3.0 | -2.4 | -2.8 | -3.5 | -3.2 | -3.3 | 0 | 0.1 | 0 | – | 0 | – |
Cytoplasm | 1.4 | 1.0 | 0 | 0.7 | 0.2 | -0.6 | -0.9 | 0 | 0 | 0 | 1.4 | 0 | – | 0 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Endosome | 0.4 | 1.7 | -0.1 | 0.6 | 1.4 | -1.4 | -1.5 | -1.4 | -1.1 | 0 | 0.3 | 0 | – | 0 | – |
Golgi | 1.0 | -1.1 | -6.2 | -5.8 | -4.5 | -8.8 | -8.7 | -7.0 | -7.5 | 0 | 1.5 | 2.6 | – | 0 | – |
Mitochondria | 0.6 | -1.9 | 1.0 | -0.5 | -3.7 | 4.5 | 3.8 | 2.3 | 4.1 | 0.7 | 0.6 | 0.7 | – | -6.3 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Nuclear Periphery | -2.6 | -1.5 | 1.7 | 1.4 | 0.6 | 1.3 | 1.4 | 1.5 | 2.4 | 0 | 0 | 0 | – | 0 | – |
Nucleolus | 0 | 2.4 | 3.5 | 2.7 | 2.9 | 3.3 | 3.3 | 3.7 | 3.2 | 0 | 0 | 0 | – | 0 | – |
Peroxisomes | -2.7 | -2.1 | -5.3 | -6.6 | -5.1 | -5.4 | -4.6 | -5.4 | -6.2 | 0 | -1.7 | -2.9 | – | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 1.1 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Vacuole | -0.1 | 2.3 | 0.3 | 1.3 | 3.2 | -1.7 | -2.6 | -1.4 | -1.6 | 0 | -0.3 | -1.0 | – | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.7051 | 8.1826 | 8.2354 | 7.5897 | 7.4628 | 8.1576 | 10.2763 | 10.796 | 10.1238 | 10.6787 | 10.8906 | 10.5086 | 9.7652 | 9.6255 | 9.5403 | 9.1238 | 8.5045 | 9.3463 |
Actin | 0.0073 | 0.004 | 0.0011 | 0.0011 | 0.0004 | 0.0005 | 0.0034 | 0.0011 | 0.018 | 0.0013 | 0.001 | 0.0015 | 0.0099 | 0.0011 | 0.0037 | 0.0007 | 0.0002 | 0.0005 |
Bud | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0062 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0004 | 0.0001 | 0.0014 | 0.0002 | 0 | 0 | 0.0004 | 0.0001 | 0.0009 | 0.0321 | 0.0021 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 |
Bud Site | 0.0002 | 0.0002 | 0.001 | 0.0001 | 0 | 0 | 0.0005 | 0.0002 | 0.0014 | 0.0006 | 0.0001 | 0 | 0.0018 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0023 | 0.0019 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.012 | 0.0006 | 0.0008 | 0.0004 | 0.0001 | 0.0002 | 0.0014 | 0.0008 | 0.0013 | 0.0066 | 0.0008 | 0.0003 |
Eisosomes | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0057 | 0.0065 | 0.0006 | 0.0005 | 0.0035 | 0.0027 | 0.0193 | 0.0016 | 0.005 | 0.0028 | 0.0004 | 0.002 | 0.0057 | 0.0026 | 0.0031 | 0.0118 | 0.0048 | 0.0009 |
Golgi | 0.0117 | 0.0197 | 0.0024 | 0.001 | 0.0265 | 0.0091 | 0.0198 | 0.0026 | 0.004 | 0.0049 | 0.0008 | 0.0045 | 0.0082 | 0.0062 | 0.0143 | 0.0124 | 0.0078 | 0.0092 |
Lipid Particles | 0.0227 | 0.0084 | 0.0013 | 0.0008 | 0.0112 | 0.0035 | 0.0299 | 0.0057 | 0.0047 | 0.0012 | 0.0002 | 0.0014 | 0.008 | 0.0078 | 0.0087 | 0.0028 | 0.0053 | 0.0058 |
Mitochondria | 0.9269 | 0.9493 | 0.9841 | 0.9906 | 0.9528 | 0.977 | 0.8606 | 0.9764 | 0.9509 | 0.934 | 0.9931 | 0.9752 | 0.9435 | 0.9733 | 0.9508 | 0.9589 | 0.9713 | 0.9798 |
None | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0056 | 0 | 0 | 0 | 0 | 0.0001 | 0.0013 | 0 | 0.0012 | 0.0002 | 0 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleolus | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0.0018 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0017 | 0 | 0.0002 | 0.0003 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0108 | 0.0063 | 0.004 | 0.0039 | 0.0033 | 0.0012 | 0.024 | 0.0073 | 0.0081 | 0.002 | 0.001 | 0.0089 | 0.0089 | 0.004 | 0.0144 | 0.0043 | 0.0038 | 0.0013 |
Punctate Nuclear | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0008 | 0.0008 | 0.0007 | 0.0003 | 0.0002 | 0.0006 | 0.0109 | 0.0008 | 0.0011 | 0.007 | 0.0002 | 0.0007 | 0.0028 | 0.0011 | 0.0006 | 0.0005 | 0.0012 | 0.0002 |
Vacuole Periphery | 0.0031 | 0.0022 | 0.0014 | 0.0009 | 0.0011 | 0.0046 | 0.0124 | 0.0031 | 0.0017 | 0.0056 | 0.0003 | 0.005 | 0.0054 | 0.0021 | 0.0023 | 0.0009 | 0.0043 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.1114 | 18.6814 | 10.1657 | 17.4606 | 7.2253 | 13.9242 | 18.8887 | 26.3471 | 26.6187 | 18.295 |
Translational Efficiency | 1.4245 | 1.6822 | 2.5644 | 1.2616 | 3.4922 | 2.7657 | 2.036 | 1.7478 | 1.6234 | 2.1954 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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