Standard name | |
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Human Ortholog | |
Description | SUMO E3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 1 | 3 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 0 |
Bud | 1 | 0 | 1 | 0 | 0 | 1 | 3 | 4 | 5 | 5 | 6 | 4 | 1 | 1 | 0 | 1 | 1 | 1 | 3 | 2 | 3 | 1 | 6 | 7 |
Bud Neck | 0 | 0 | 5 | 0 | 6 | 0 | 9 | 11 | 1 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 4 | 2 | 1 | 5 | 2 |
Bud Site | 0 | 1 | 0 | 1 | 6 | 4 | 8 | 4 | 10 | 18 | 10 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 10 | 22 | 11 | 17 | 20 | 31 | 35 | 20 | 13 | 15 | 23 | 19 | 46 | 48 | 62 | 45 | 61 | 1 | 3 | 3 | 0 | 5 | 5 |
Endoplasmic Reticulum | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 23 | 15 | 16 | 1 | 0 | 3 | 1 | 6 | 0 |
Endosome | 5 | 0 | 5 | 8 | 2 | 7 | 3 | 2 | 1 | 1 | 0 | 1 | 0 | 3 | 5 | 16 | 23 | 8 | 10 | 5 | 10 | 2 | 6 | 8 |
Golgi | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 6 | 0 | 0 | 1 | 0 | 0 |
Mitochondria | 48 | 72 | 15 | 15 | 14 | 66 | 147 | 102 | 155 | 166 | 198 | 139 | 2 | 2 | 2 | 22 | 15 | 38 | 7 | 6 | 7 | 4 | 12 | 11 |
Nucleus | 293 | 261 | 293 | 165 | 213 | 176 | 215 | 234 | 177 | 158 | 151 | 198 | 240 | 289 | 312 | 397 | 342 | 356 | 281 | 266 | 273 | 148 | 225 | 186 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 2 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 3 | 1 | 2 | 4 | 2 | 2 | 3 | 6 | 1 | 6 | 8 | 9 | 13 | 36 | 22 |
Peroxisomes | 4 | 1 | 1 | 3 | 2 | 4 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 3 | 6 | 1 | 3 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 2 | 3 | 15 | 5 | 16 | 8 | 14 | 19 | 4 | 7 | 5 | 15 | 6 | 8 | 8 | 26 | 24 | 13 | 2 | 5 | 10 | 4 | 10 | 5 |
Vac/Vac Membrane | 7 | 16 | 9 | 2 | 4 | 2 | 5 | 5 | 1 | 1 | 2 | 4 | 0 | 4 | 7 | 42 | 38 | 31 | 18 | 34 | 42 | 38 | 55 | 57 |
Unique Cell Count | 342 | 309 | 345 | 194 | 257 | 245 | 315 | 334 | 252 | 236 | 250 | 285 | 261 | 342 | 366 | 534 | 467 | 462 | 360 | 342 | 380 | 226 | 387 | 320 |
Labelled Cell Count | 379 | 368 | 368 | 213 | 282 | 292 | 436 | 419 | 376 | 376 | 392 | 404 | 273 | 356 | 386 | 600 | 517 | 529 | 360 | 342 | 380 | 226 | 387 | 320 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 4.5 | 5.6 | 5.0 | 5.1 | 4.4 | 4.2 | 4.5 | 3.8 | 3.6 | 3.3 | 4.1 | 6.2 | 6.3 | 6.4 | 6.9 | 7.2 | 6.3 | 6.0 | 6.3 | 6.8 |
Std Deviation (1e-4) | 1.1 | 0.6 | 1.2 | 1.2 | 1.2 | 1.2 | 1.1 | 1.3 | 0.9 | 1.1 | 0.9 | 1.2 | 1.6 | 1.7 | 1.9 | 1.6 | 1.4 | 1.2 | 1.8 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.13 | -0.37 | -0.43 | -0.32 | -0.58 | -0.62 | -0.76 | -0.47 | 0.14 | 0.17 | 0.19 | 0.29 | 0.36 | 0.17 | 0.09 | 0.16 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.3 | 3.3 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5364 | 2.5655 | 2.6139 | 2.2604 | 2.2995 | 2.6932 | 1.3759 | 0.7799 | 0.7624 | 1.0712 | 0.8613 | 0.6656 | 0.6463 | 0.7915 | 0.4317 | 0.4478 | 0.5498 | 0.6454 |
Actin | 0.0469 | 0.005 | 0.0131 | 0.059 | 0.0032 | 0.0009 | 0.037 | 0 | 0.0101 | 0.0333 | 0.0344 | 0.0001 | 0.0063 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 |
Bud | 0.0005 | 0.0003 | 0.0003 | 0.0008 | 0.0002 | 0 | 0.0011 | 0 | 0.0003 | 0.001 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0011 |
Bud Neck | 0.0019 | 0.0006 | 0.003 | 0.0055 | 0.2575 | 0.1416 | 0.0154 | 0.0006 | 0.0003 | 0.0914 | 0.138 | 0.0833 | 0.001 | 0.0007 | 0.0001 | 0.0726 | 0.1324 | 0.0495 |
Bud Periphery | 0.0006 | 0.0002 | 0.0006 | 0.0009 | 0.0004 | 0.0001 | 0.0026 | 0 | 0.0005 | 0.0026 | 0.0009 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0005 |
Bud Site | 0.0043 | 0.0015 | 0.0102 | 0.0098 | 0.0013 | 0.0002 | 0.0051 | 0.0061 | 0.0032 | 0.0074 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0012 | 0.0004 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0013 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0062 | 0.0028 | 0.0023 | 0.0009 | 0.0135 | 0.0012 | 0.0045 | 0.0048 | 0.0015 | 0.0061 | 0.0075 | 0.0004 | 0.0013 | 0.0008 | 0.0017 | 0.0004 | 0.004 | 0.0005 |
Cytoplasmic Foci | 0.0027 | 0.0018 | 0.0024 | 0.0212 | 0.0121 | 0.0013 | 0.0245 | 0.0034 | 0.0018 | 0.0308 | 0.0093 | 0.0001 | 0.0007 | 0 | 0.0013 | 0 | 0.0032 | 0.0001 |
Eisosomes | 0.0005 | 0 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0013 | 0 | 0.0001 | 0.0006 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0013 | 0.0004 | 0.0014 | 0.0003 | 0.0016 | 0.0006 | 0.0024 | 0.0001 | 0.0008 | 0.0045 | 0.0049 | 0 | 0.0015 | 0.0002 | 0 | 0 | 0.0003 | 0 |
Endosome | 0.0022 | 0.0021 | 0.002 | 0.0069 | 0.0228 | 0.0047 | 0.0199 | 0.0015 | 0.009 | 0.0353 | 0.0351 | 0.0012 | 0.0018 | 0.0002 | 0 | 0 | 0.0005 | 0 |
Golgi | 0.0031 | 0.0019 | 0.0016 | 0.0181 | 0.0036 | 0.0011 | 0.015 | 0.0001 | 0.0036 | 0.011 | 0.0093 | 0.0005 | 0.0006 | 0 | 0 | 0 | 0.0003 | 0 |
Lipid Particles | 0.0016 | 0.0006 | 0.0013 | 0.0094 | 0.008 | 0.0072 | 0.019 | 0.0001 | 0.0064 | 0.0144 | 0.0173 | 0 | 0.0014 | 0.0001 | 0 | 0 | 0.0006 | 0 |
Mitochondria | 0.007 | 0.0009 | 0.0162 | 0.0093 | 0.005 | 0.0008 | 0.0247 | 0.0001 | 0.0043 | 0.0527 | 0.0044 | 0.0046 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0 |
None | 0.0703 | 0.0036 | 0.0198 | 0.001 | 0.0343 | 0.0107 | 0.0082 | 0.0006 | 0.0107 | 0.0009 | 0.0039 | 0.0001 | 0.0031 | 0.0029 | 0.0025 | 0 | 0.0007 | 0.0002 |
Nuclear Periphery | 0.0087 | 0.004 | 0.0058 | 0.0062 | 0.0041 | 0.0015 | 0.0174 | 0.0033 | 0.0086 | 0.005 | 0.0274 | 0.0009 | 0.0066 | 0.0065 | 0.0013 | 0.0024 | 0.0067 | 0.0004 |
Nucleolus | 0.0127 | 0.0134 | 0.0244 | 0.0114 | 0.0311 | 0.0303 | 0.0118 | 0.0097 | 0.0082 | 0.0129 | 0.0245 | 0.0188 | 0.0102 | 0.0124 | 0.0066 | 0.0097 | 0.073 | 0.0203 |
Nucleus | 0.811 | 0.9359 | 0.8685 | 0.7894 | 0.4724 | 0.7828 | 0.7585 | 0.9621 | 0.9167 | 0.6541 | 0.5953 | 0.8748 | 0.9435 | 0.9428 | 0.9305 | 0.892 | 0.4942 | 0.8782 |
Peroxisomes | 0.0041 | 0.0003 | 0.0011 | 0.0443 | 0.0044 | 0.0026 | 0.0118 | 0.0001 | 0.0012 | 0.0066 | 0.0104 | 0 | 0.0009 | 0 | 0 | 0 | 0.0002 | 0 |
Punctate Nuclear | 0.0131 | 0.0242 | 0.0239 | 0.0041 | 0.1229 | 0.0108 | 0.0133 | 0.0068 | 0.0096 | 0.0086 | 0.073 | 0.0144 | 0.0193 | 0.0329 | 0.0552 | 0.0223 | 0.2809 | 0.0485 |
Vacuole | 0.0007 | 0.0002 | 0.0012 | 0.0006 | 0.001 | 0.0008 | 0.0037 | 0.0003 | 0.0016 | 0.0152 | 0.0013 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0021 | 0.0001 | 0.0011 | 0.0042 | 0.0016 | 0.0002 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.1282 | 8.7193 | 7.0093 | 10.7529 | 9.2213 | 5.2804 | 10.561 | 10.0411 | 10.418 | 12.0998 |
Translational Efficiency | 0.6126 | 0.5629 | 0.7983 | 0.3959 | 0.4526 | 1.0813 | 0.4876 | 0.6606 | 0.6958 | 0.5171 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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