Standard name
Human Ortholog
Description SUMO E3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.07 0.08 0.1 0.1 0.08 0.06 0.06 0.08 0.07 0.13 0.13 0.12 0.1 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.23 0 0.08 0.05 0.27 0.47 0.31 0.62 0.7 0.79 0.49 0 0 0 0 0 0.08 0 0 0 0 0 0
Nucleus 0.86 0.84 0.85 0.85 0.83 0.72 0.68 0.7 0.7 0.67 0.6 0.69 0.92 0.85 0.85 0.74 0.73 0.77 0.78 0.78 0.72 0.66 0.58 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0 0.06 0 0 0 0.05 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.07 0.05 0.1 0.11 0.17 0.14 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 3 1 0 1 0 0 1 0 0 0 0 0 0 0 1 1 1 1 1 2 0
Bud 1 0 1 0 0 1 3 4 5 5 6 4 1 1 0 1 1 1 3 2 3 1 6 7
Bud Neck 0 0 5 0 6 0 9 11 1 3 2 2 0 0 0 0 0 0 9 4 2 1 5 2
Bud Site 0 1 0 1 6 4 8 4 10 18 10 11 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 13 10 22 11 17 20 31 35 20 13 15 23 19 46 48 62 45 61 1 3 3 0 5 5
Endoplasmic Reticulum 3 2 0 0 1 0 0 2 0 0 0 1 0 1 1 23 15 16 1 0 3 1 6 0
Endosome 5 0 5 8 2 7 3 2 1 1 0 1 0 3 5 16 23 8 10 5 10 2 6 8
Golgi 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 2 1 6 0 0 1 0 0
Mitochondria 48 72 15 15 14 66 147 102 155 166 198 139 2 2 2 22 15 38 7 6 7 4 12 11
Nucleus 293 261 293 165 213 176 215 234 177 158 151 198 240 289 312 397 342 356 281 266 273 148 225 186
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 3 0 1 0 0 2 0 2 2
Nucleolus 2 0 0 0 0 3 0 0 2 3 1 2 4 2 2 3 6 1 6 8 9 13 36 22
Peroxisomes 4 1 1 3 2 4 0 1 0 0 0 2 0 0 1 3 6 1 3 0 1 0 0 0
SpindlePole 2 3 15 5 16 8 14 19 4 7 5 15 6 8 8 26 24 13 2 5 10 4 10 5
Vac/Vac Membrane 7 16 9 2 4 2 5 5 1 1 2 4 0 4 7 42 38 31 18 34 42 38 55 57
Unique Cell Count 342 309 345 194 257 245 315 334 252 236 250 285 261 342 366 534 467 462 360 342 380 226 387 320
Labelled Cell Count 379 368 368 213 282 292 436 419 376 376 392 404 273 356 386 600 517 529 360 342 380 226 387 320


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.5 5.6 5.0 5.1 4.4 4.2 4.5 3.8 3.6 3.3 4.1 6.2 6.3 6.4 6.9 7.2 6.3 6.0 6.3 6.8
Std Deviation (1e-4) 1.1 0.6 1.2 1.2 1.2 1.2 1.1 1.3 0.9 1.1 0.9 1.2 1.6 1.7 1.9 1.6 1.4 1.2 1.8 2.1 2.2
Intensity Change (Log2) -0.16 -0.13 -0.37 -0.43 -0.32 -0.58 -0.62 -0.76 -0.47 0.14 0.17 0.19 0.29 0.36 0.17 0.09 0.16 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.3 3.3 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5364 2.5655 2.6139 2.2604 2.2995 2.6932 1.3759 0.7799 0.7624 1.0712 0.8613 0.6656 0.6463 0.7915 0.4317 0.4478 0.5498 0.6454
Actin 0.0469 0.005 0.0131 0.059 0.0032 0.0009 0.037 0 0.0101 0.0333 0.0344 0.0001 0.0063 0 0 0.0001 0.0005 0.0001
Bud 0.0005 0.0003 0.0003 0.0008 0.0002 0 0.0011 0 0.0003 0.001 0.0004 0 0.0001 0 0.0003 0 0.0002 0.0011
Bud Neck 0.0019 0.0006 0.003 0.0055 0.2575 0.1416 0.0154 0.0006 0.0003 0.0914 0.138 0.0833 0.001 0.0007 0.0001 0.0726 0.1324 0.0495
Bud Periphery 0.0006 0.0002 0.0006 0.0009 0.0004 0.0001 0.0026 0 0.0005 0.0026 0.0009 0.0001 0.0001 0 0 0 0.0002 0.0005
Bud Site 0.0043 0.0015 0.0102 0.0098 0.0013 0.0002 0.0051 0.0061 0.0032 0.0074 0.001 0.0001 0.0001 0.0001 0.0001 0 0.0012 0.0004
Cell Periphery 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0007 0 0.0001 0.0013 0.0001 0 0 0 0 0 0.0001 0
Cytoplasm 0.0062 0.0028 0.0023 0.0009 0.0135 0.0012 0.0045 0.0048 0.0015 0.0061 0.0075 0.0004 0.0013 0.0008 0.0017 0.0004 0.004 0.0005
Cytoplasmic Foci 0.0027 0.0018 0.0024 0.0212 0.0121 0.0013 0.0245 0.0034 0.0018 0.0308 0.0093 0.0001 0.0007 0 0.0013 0 0.0032 0.0001
Eisosomes 0.0005 0 0.0002 0.0004 0.0002 0.0002 0.0013 0 0.0001 0.0006 0.0002 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0013 0.0004 0.0014 0.0003 0.0016 0.0006 0.0024 0.0001 0.0008 0.0045 0.0049 0 0.0015 0.0002 0 0 0.0003 0
Endosome 0.0022 0.0021 0.002 0.0069 0.0228 0.0047 0.0199 0.0015 0.009 0.0353 0.0351 0.0012 0.0018 0.0002 0 0 0.0005 0
Golgi 0.0031 0.0019 0.0016 0.0181 0.0036 0.0011 0.015 0.0001 0.0036 0.011 0.0093 0.0005 0.0006 0 0 0 0.0003 0
Lipid Particles 0.0016 0.0006 0.0013 0.0094 0.008 0.0072 0.019 0.0001 0.0064 0.0144 0.0173 0 0.0014 0.0001 0 0 0.0006 0
Mitochondria 0.007 0.0009 0.0162 0.0093 0.005 0.0008 0.0247 0.0001 0.0043 0.0527 0.0044 0.0046 0.0007 0.0001 0.0002 0.0001 0.0005 0
None 0.0703 0.0036 0.0198 0.001 0.0343 0.0107 0.0082 0.0006 0.0107 0.0009 0.0039 0.0001 0.0031 0.0029 0.0025 0 0.0007 0.0002
Nuclear Periphery 0.0087 0.004 0.0058 0.0062 0.0041 0.0015 0.0174 0.0033 0.0086 0.005 0.0274 0.0009 0.0066 0.0065 0.0013 0.0024 0.0067 0.0004
Nucleolus 0.0127 0.0134 0.0244 0.0114 0.0311 0.0303 0.0118 0.0097 0.0082 0.0129 0.0245 0.0188 0.0102 0.0124 0.0066 0.0097 0.073 0.0203
Nucleus 0.811 0.9359 0.8685 0.7894 0.4724 0.7828 0.7585 0.9621 0.9167 0.6541 0.5953 0.8748 0.9435 0.9428 0.9305 0.892 0.4942 0.8782
Peroxisomes 0.0041 0.0003 0.0011 0.0443 0.0044 0.0026 0.0118 0.0001 0.0012 0.0066 0.0104 0 0.0009 0 0 0 0.0002 0
Punctate Nuclear 0.0131 0.0242 0.0239 0.0041 0.1229 0.0108 0.0133 0.0068 0.0096 0.0086 0.073 0.0144 0.0193 0.0329 0.0552 0.0223 0.2809 0.0485
Vacuole 0.0007 0.0002 0.0012 0.0006 0.001 0.0008 0.0037 0.0003 0.0016 0.0152 0.0013 0.0004 0.0003 0.0001 0 0.0001 0.0003 0.0001
Vacuole Periphery 0.0004 0.0001 0.0006 0.0004 0.0004 0.0002 0.0021 0.0001 0.0011 0.0042 0.0016 0.0002 0.0003 0.0002 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1282 8.7193 7.0093 10.7529 9.2213 5.2804 10.561 10.0411 10.418 12.0998
Translational Efficiency 0.6126 0.5629 0.7983 0.3959 0.4526 1.0813 0.4876 0.6606 0.6958 0.5171

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description SUMO E3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p
Localization
Cell Percentages nucleus (89%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Siz1

Siz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Siz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available