Standard name | |
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Human Ortholog | |
Description | Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 3 |
Bud | 0 | 2 | 1 | 10 | 4 | 13 | 13 | 20 | 12 | 5 | 14 | 13 | 0 | 0 | 3 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 5 |
Bud Neck | 1 | 1 | 10 | 1 | 5 | 2 | 4 | 5 | 2 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 1 | 2 | 3 | 2 | 10 | 17 | 10 | 8 | 4 | 18 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 65 | 62 | 86 | 105 | 119 | 147 | 151 | 180 | 83 | 100 | 46 | 72 | 49 | 78 | 105 | 71 | 60 | 57 | 47 | 62 | 52 | 20 | 41 | 49 |
Endoplasmic Reticulum | 3 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 13 | 9 | 18 | 2 | 0 | 2 | 0 | 1 | 1 |
Endosome | 47 | 6 | 0 | 0 | 12 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 15 | 8 | 6 | 38 | 44 | 53 | 49 | 10 | 19 | 1 | 6 | 12 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 12 | 65 | 66 | 98 | 85 | 212 | 301 | 331 | 224 | 288 | 196 | 276 | 18 | 10 | 37 | 7 | 5 | 20 | 26 | 18 | 51 | 2 | 8 | 16 |
Nucleus | 10 | 1 | 3 | 4 | 8 | 11 | 29 | 36 | 26 | 38 | 20 | 31 | 8 | 5 | 7 | 23 | 14 | 37 | 16 | 11 | 10 | 2 | 4 | 7 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 6 | 3 | 0 | 1 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 2 | 2 | 0 | 1 | 1 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 20 | 2 | 3 | 12 | 41 | 14 | 34 | 34 | 11 | 19 | 15 | 19 | 1 | 1 | 2 | 9 | 12 | 13 | 81 | 28 | 30 | 8 | 22 | 43 |
Vac/Vac Membrane | 45 | 29 | 1 | 3 | 10 | 10 | 11 | 7 | 8 | 5 | 5 | 5 | 23 | 20 | 17 | 45 | 70 | 57 | 31 | 28 | 27 | 7 | 23 | 22 |
Unique Cell Count | 178 | 140 | 162 | 202 | 244 | 338 | 437 | 508 | 295 | 361 | 238 | 332 | 109 | 116 | 151 | 173 | 171 | 205 | 286 | 178 | 218 | 48 | 121 | 181 |
Labelled Cell Count | 207 | 168 | 177 | 236 | 287 | 413 | 558 | 636 | 380 | 465 | 304 | 444 | 114 | 123 | 179 | 207 | 215 | 261 | 286 | 178 | 218 | 48 | 121 | 181 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 3.1 | 3.0 | 3.3 | 3.6 | 3.2 | 2.9 | 3.0 | 2.7 | 2.6 | 2.7 | 2.3 | 4.1 | 4.2 | 3.7 | 5.4 | 5.6 | 5.8 | 3.9 | 3.9 | 5.1 |
Std Deviation (1e-4) | 0.8 | 0.8 | 0.8 | 1.7 | 1.8 | 1.5 | 0.9 | 1.3 | 1.2 | 1.1 | 1.3 | 1.0 | 1.5 | 1.3 | 1.3 | 1.3 | 1.4 | 1.5 | 1.5 | 1.2 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.27 | 0.07 | -0.09 | -0.02 | -0.18 | -0.23 | -0.14 | -0.38 | 0.43 | 0.48 | 0.28 | 0.84 | 0.89 | 0.94 | 0.36 | 0.35 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.7 | 9.0 | 7.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0272 | 0.1953 | -0.1966 | -0.2217 | 0.0367 | -0.1442 | 0.2517 | 0.3631 | 0.3736 | 0.8076 | 0.8221 | 0.2978 | 1.0816 | 1.0265 | 1.2407 | 1.1387 | 0.6213 | 1.115 |
Actin | 0.0266 | 0.0003 | 0.0076 | 0.0016 | 0.0056 | 0.0019 | 0.0105 | 0.0005 | 0.0081 | 0.0293 | 0.0055 | 0.0009 | 0.0149 | 0.0005 | 0.0166 | 0.0011 | 0.0003 | 0.0004 |
Bud | 0.0008 | 0.0004 | 0.0003 | 0.0003 | 0.0009 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.001 | 0.0008 | 0.0002 | 0.0003 | 0.0047 | 0.0071 | 0.0017 | 0.0001 | 0.0004 |
Bud Neck | 0.0123 | 0.0039 | 0.0026 | 0.0437 | 0.025 | 0.0103 | 0.0061 | 0.0006 | 0.003 | 0.019 | 0.0027 | 0.0034 | 0.0022 | 0.0007 | 0.0015 | 0.0005 | 0.0011 | 0.002 |
Bud Periphery | 0.0024 | 0.0008 | 0.0005 | 0.0006 | 0.002 | 0.0003 | 0.001 | 0.0001 | 0.001 | 0.0017 | 0.0018 | 0.0011 | 0.0004 | 0.0007 | 0.0017 | 0.0079 | 0.0001 | 0.0001 |
Bud Site | 0.0043 | 0.0106 | 0.0022 | 0.0021 | 0.0028 | 0.0003 | 0.0085 | 0.0008 | 0.0057 | 0.0149 | 0.001 | 0.0002 | 0.0102 | 0.0055 | 0.0018 | 0.0002 | 0.0001 | 0.0002 |
Cell Periphery | 0.0008 | 0.0004 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.161 | 0.1857 | 0.1667 | 0.2084 | 0.122 | 0.1928 | 0.1064 | 0.1803 | 0.1208 | 0.0747 | 0.1421 | 0.1223 | 0.1426 | 0.1472 | 0.1241 | 0.0679 | 0.1369 | 0.1547 |
Cytoplasmic Foci | 0.0463 | 0.0298 | 0.0488 | 0.0246 | 0.0511 | 0.0344 | 0.0398 | 0.0291 | 0.0246 | 0.0186 | 0.0287 | 0.0097 | 0.0328 | 0.022 | 0.0205 | 0.0082 | 0.021 | 0.0407 |
Eisosomes | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0091 | 0.0067 | 0.0098 | 0.0064 | 0.0067 | 0.0076 | 0.005 | 0.003 | 0.0045 | 0.0033 | 0.0067 | 0.002 | 0.0051 | 0.0099 | 0.005 | 0.0042 | 0.0026 | 0.0025 |
Endosome | 0.0392 | 0.0122 | 0.0208 | 0.0211 | 0.0351 | 0.0158 | 0.0305 | 0.0074 | 0.0144 | 0.0155 | 0.0272 | 0.0038 | 0.0102 | 0.0071 | 0.0079 | 0.0042 | 0.0169 | 0.0052 |
Golgi | 0.0074 | 0.0006 | 0.0015 | 0.0005 | 0.0061 | 0.001 | 0.0057 | 0.0003 | 0.0037 | 0.0082 | 0.0061 | 0.0003 | 0.0043 | 0.0004 | 0.0013 | 0.0003 | 0.0028 | 0.0002 |
Lipid Particles | 0.0292 | 0.0055 | 0.0107 | 0.0014 | 0.0302 | 0.0056 | 0.0179 | 0.0011 | 0.0098 | 0.0256 | 0.0316 | 0.0011 | 0.0178 | 0.0014 | 0.0029 | 0.0031 | 0.0044 | 0.0029 |
Mitochondria | 0.0148 | 0.0007 | 0.002 | 0.0012 | 0.0039 | 0.0049 | 0.0043 | 0.0004 | 0.0057 | 0.0193 | 0.0559 | 0.0009 | 0.0022 | 0.001 | 0.0113 | 0.0012 | 0.0228 | 0.0007 |
None | 0.2734 | 0.4792 | 0.3226 | 0.2568 | 0.3881 | 0.2529 | 0.3915 | 0.5156 | 0.3901 | 0.3481 | 0.3177 | 0.3882 | 0.4264 | 0.5439 | 0.5951 | 0.6529 | 0.5571 | 0.5275 |
Nuclear Periphery | 0.0244 | 0.0132 | 0.025 | 0.0325 | 0.0193 | 0.0207 | 0.0254 | 0.0063 | 0.0221 | 0.0172 | 0.0319 | 0.0108 | 0.0143 | 0.0083 | 0.0103 | 0.015 | 0.0038 | 0.0064 |
Nucleolus | 0.0074 | 0.0131 | 0.0066 | 0.0086 | 0.0062 | 0.0101 | 0.0079 | 0.0056 | 0.0082 | 0.0078 | 0.0057 | 0.0067 | 0.0064 | 0.0046 | 0.0031 | 0.0028 | 0.0035 | 0.0044 |
Nucleus | 0.224 | 0.1655 | 0.2041 | 0.2553 | 0.1457 | 0.2863 | 0.1963 | 0.1402 | 0.2041 | 0.2191 | 0.1937 | 0.3166 | 0.1525 | 0.1467 | 0.1248 | 0.1529 | 0.116 | 0.1556 |
Peroxisomes | 0.0174 | 0.0006 | 0.0084 | 0.0006 | 0.0163 | 0.0027 | 0.0139 | 0.0007 | 0.005 | 0.0128 | 0.0214 | 0.0005 | 0.0378 | 0.001 | 0.0024 | 0.0009 | 0.0013 | 0.0004 |
Punctate Nuclear | 0.0889 | 0.0666 | 0.1541 | 0.1271 | 0.1263 | 0.1483 | 0.1235 | 0.1059 | 0.1648 | 0.1585 | 0.1115 | 0.1302 | 0.1163 | 0.0911 | 0.0597 | 0.0729 | 0.1061 | 0.0943 |
Vacuole | 0.0068 | 0.0037 | 0.0046 | 0.0054 | 0.0044 | 0.0028 | 0.0036 | 0.0016 | 0.0025 | 0.0031 | 0.0037 | 0.0008 | 0.0024 | 0.0025 | 0.002 | 0.0014 | 0.0018 | 0.0012 |
Vacuole Periphery | 0.0025 | 0.0004 | 0.0008 | 0.0008 | 0.0018 | 0.0012 | 0.0013 | 0.0002 | 0.0007 | 0.0012 | 0.004 | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.0005 | 0.0012 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.188 | 12.1007 | 5.9006 | 10.4934 | 8.2738 | 6.9577 | 9.0888 | 11.089 | 13.1162 | 11.2884 |
Translational Efficiency | 0.4566 | 0.2421 | 0.6114 | 0.4046 | 0.3513 | 0.4118 | 0.4451 | 0.3779 | 0.3488 | 0.3791 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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