Standard name
Human Ortholog
Description Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0.09 0.21 0.09 0.1 0.07 0.08 0.11 0.11
Bud 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.32 0.28 0.26 0.15 0.09 0.05 – 0.07 0.07 0.06 0.06 0.07 0.21 0.12 0.12 0.2 0.19 0.14 0.07 0 0.06 0 0 0
Endoplasmic Reticulum 0.09 0.2 0.16 0.12 0.17 0.08 – 0.05 0 0.08 0.1 0.07 0.34 0.34 0.17 0.06 0 0.06 0.15 0.15 0.13 0.11 0.11 0.1
Endosome 0.22 0.16 0 0 0.2 0.05 – 0.16 0 0 0 0 0.1 0.23 0.42 0.49 0.4 0.39 0.19 0.25 0.12 0.23 0.17 0.21
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0.11 0.06 0.11 0 0.1 0.13 0.11
Mitochondria 0.2 0.09 0.66 0.8 0.43 0.78 – 0.58 0.81 0.77 0.84 0.84 0.11 0.17 0.11 0.12 0.11 0.1 0.19 0.16 0.43 0.22 0.21 0.22
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.06 0.06 – 0 0.05 0.06 0.06 0.05 0 0 0 0 0 0 0.08 0.06 0.05 0.09 0.08 0.07
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.1 0.14 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0.29 0.3 0.06 0.08 0.22 0.14 – 0.22 0.18 0.17 0.11 0.08 0.34 0.16 0.27 0.25 0.21 0.18 0.05 0 0 0.05 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 12 4 2 10 4 – 16 0 4 2 10 7 20 5 6 15 20 43 22 16 10 37 39
Bud 11 1 7 9 15 9 – 26 25 20 20 28 3 3 6 0 3 3 5 2 1 2 3 6
Bud Neck 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 1 0 4 1 1 0 1 4
Bud Site 1 0 0 0 0 4 – 4 4 3 6 5 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 1 0 0 1 0 – 1 0 3 0 3 0 1 0 1 3 0 0 0 0 0 0 0
Cytoplasm 119 48 45 40 43 28 – 58 32 37 39 51 41 40 39 27 33 13 35 8 15 3 13 11
Endoplasmic Reticulum 34 35 28 32 83 42 – 40 15 47 59 56 67 111 56 8 6 6 76 34 30 13 35 35
Endosome 84 28 0 5 97 26 – 132 16 24 7 11 19 76 136 68 71 37 96 57 29 29 55 74
Golgi 0 3 0 2 3 2 – 0 0 0 1 0 1 11 9 6 13 10 31 26 3 13 42 37
Mitochondria 76 16 114 211 203 408 – 465 397 439 517 656 22 56 37 16 20 9 98 37 105 28 68 79
Nucleus 0 0 0 1 3 2 – 8 3 2 10 7 1 0 1 0 4 3 0 0 0 0 1 0
Nuclear Periphery 1 0 0 9 29 33 – 26 22 37 37 36 6 5 5 0 2 0 42 12 12 12 24 26
Nucleolus 0 0 0 0 7 7 – 28 11 6 8 15 0 2 2 0 1 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 7 3 – 14 1 2 3 1 1 7 15 4 10 0 15 7 8 3 13 9
SpindlePole 4 2 2 0 5 6 – 27 5 7 5 16 1 11 18 14 24 9 16 4 5 3 3 7
Vac/Vac Membrane 108 52 10 21 107 73 – 176 86 98 68 64 68 51 87 34 37 17 27 9 7 6 18 18
Unique Cell Count 376 173 173 265 477 520 804 488 573 614 784 199 327 327 138 176 94 504 232 243 129 326 358
Labelled Cell Count 450 199 210 332 613 647 1021 617 729 783 959 237 394 416 184 243 128 504 232 243 129 326 358


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.4 4.2 5.0 6.2 5.2 – 5.4 4.6 4.9 4.7 4.6 6.8 7.0 6.9 7.9 7.7 7.6 6.1 7.0 6.8
Std Deviation (1e-4) 1.1 1.4 0.8 1.3 1.2 1.1 – 1.2 1.3 0.9 1.0 0.9 1.1 1.3 1.1 1.5 1.7 1.4 1.1 1.4 1.3
Intensity Change (Log2) – – – 0.25 0.56 0.32 – 0.37 0.14 0.21 0.15 0.12 0.69 0.74 0.72 0.92 0.89 0.87 0.54 0.73 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0.7 1.9 0 0 2.6 5.2
Bud -0.4 -0.6 0 – -0.5 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.8 -5.6 -7.7 – -7.3 -6.9 -7.2 -7.4 -7.7 -1.2 -3.9 -4.0 -1.3 -1.6 -2.3
Endoplasmic Reticulum -1.2 0.4 -3.1 – -5.3 0 0 0 0 3.9 4.2 0.3 -2.8 -4.0 -2.3
Endosome 0 6.4 3.0 – 5.7 0 0 0 0 4.2 6.9 9.9 10.4 9.4 8.9
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 3.6 0
Mitochondria 3.2 -5.3 3.3 – -2.0 0 0 0 0 -11.0 -11.0 -12.6 -9.6 -10.5 -8.8
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 3.3 3.4 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 2.9 0 0 0
SpindlePole 0 0 0 – 1.5 0 0 0 0 0 0 2.4 3.6 4.4 0
Vacuole 0.9 4.9 2.9 – 4.9 3.8 3.7 2.1 1.1 6.7 3.2 5.6 4.7 4.2 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8324 3.4178 3.0651 3.025 2.44 2.991 2.2622 2.4619 2.3284 2.65 1.9737 2.2002 3.8356 4.6086 4.2539 4.4188 3.8187 4.0739
Actin 0.0252 0.007 0.0134 0.0082 0.0433 0.0107 0.0574 0.0091 0.0171 0.008 0.0274 0.0087 0.0605 0.0124 0.0325 0.036 0.0266 0.0169
Bud 0.0005 0.0004 0.0004 0.0004 0.0009 0.0009 0.0008 0.0006 0.0005 0.0011 0.0005 0.0002 0.0006 0.0004 0.0004 0.0007 0.0004 0.0003
Bud Neck 0.0026 0.0012 0.0006 0.0008 0.0085 0.0012 0.0015 0.0004 0.0034 0.0016 0.0007 0.0086 0.0021 0.0007 0.0007 0.0013 0.0008 0.0009
Bud Periphery 0.0009 0.0004 0.0005 0.0008 0.0018 0.0008 0.0012 0.0003 0.0007 0.0014 0.0006 0.0003 0.0012 0.0005 0.0007 0.0009 0.0005 0.0008
Bud Site 0.0034 0.0027 0.0009 0.0005 0.004 0.0004 0.0055 0.0033 0.0044 0.006 0.0008 0.0003 0.0026 0.0038 0.0016 0.0017 0.0015 0.0003
Cell Periphery 0.0006 0.0002 0.0002 0.0002 0.0006 0.0002 0.0004 0.0002 0.0004 0.0003 0.0002 0.0002 0.0004 0.0004 0.0002 0.0003 0.0003 0.0002
Cytoplasm 0.0223 0.1039 0.0245 0.0254 0.018 0.041 0.0366 0.0895 0.0513 0.0225 0.0142 0.0736 0.0229 0.1149 0.0518 0.0593 0.1002 0.0266
Cytoplasmic Foci 0.0564 0.0503 0.0615 0.1287 0.0592 0.0465 0.0438 0.0534 0.0368 0.0666 0.12 0.0583 0.0628 0.0411 0.0707 0.1048 0.072 0.0493
Eisosomes 0.0007 0.0001 0.0001 0.0001 0.0011 0.0001 0.0004 0 0.0001 0.0001 0.0001 0.0001 0.0007 0.0001 0.0002 0.0002 0.0001 0.0002
Endoplasmic Reticulum 0.0782 0.1518 0.1057 0.0747 0.1013 0.0855 0.1363 0.1704 0.1923 0.1102 0.0614 0.1102 0.1385 0.2005 0.1543 0.0844 0.0967 0.1203
Endosome 0.19 0.1751 0.239 0.2902 0.2951 0.2045 0.1809 0.242 0.2151 0.2835 0.4368 0.2036 0.1411 0.1683 0.2222 0.2168 0.2318 0.2316
Golgi 0.0592 0.0348 0.0693 0.1085 0.0897 0.0481 0.0572 0.0433 0.0451 0.0812 0.1082 0.0373 0.0369 0.0258 0.0857 0.074 0.0629 0.0504
Lipid Particles 0.1911 0.053 0.1134 0.1528 0.1277 0.128 0.0744 0.049 0.0515 0.0342 0.0687 0.08 0.1684 0.0755 0.0876 0.1492 0.1956 0.0647
Mitochondria 0.0338 0.0203 0.027 0.0573 0.0542 0.0273 0.0411 0.0129 0.0306 0.0336 0.0414 0.0185 0.023 0.0123 0.027 0.0244 0.023 0.0243
None 0.0221 0.0101 0.0096 0.0013 0.0062 0.0066 0.0197 0.0128 0.0064 0.0039 0.0015 0.0136 0.0291 0.0284 0.0104 0.0043 0.003 0.0138
Nuclear Periphery 0.2524 0.3077 0.2755 0.0613 0.1164 0.3109 0.2678 0.2566 0.2764 0.2449 0.0714 0.31 0.2249 0.2588 0.1892 0.1181 0.0818 0.3299
Nucleolus 0.001 0.0006 0.0004 0.0002 0.0048 0.0009 0.0011 0.001 0.0006 0.0006 0.0002 0.0006 0.0008 0.0004 0.0006 0.0006 0.0003 0.0004
Nucleus 0.0066 0.0331 0.0077 0.0044 0.0106 0.0159 0.0094 0.0114 0.0112 0.0133 0.0029 0.0146 0.0068 0.0146 0.007 0.0103 0.0138 0.01
Peroxisomes 0.0165 0.0045 0.0124 0.0569 0.0136 0.0201 0.0205 0.0057 0.0126 0.0212 0.0166 0.016 0.0458 0.0068 0.0216 0.0702 0.0472 0.0227
Punctate Nuclear 0.009 0.0137 0.0058 0.0025 0.0132 0.0187 0.0156 0.0057 0.0055 0.0106 0.0034 0.0176 0.0105 0.0068 0.0086 0.019 0.0107 0.0056
Vacuole 0.0082 0.0114 0.0097 0.0101 0.0107 0.0097 0.0082 0.0136 0.0137 0.0196 0.0115 0.0088 0.0066 0.0122 0.0101 0.0066 0.0158 0.0098
Vacuole Periphery 0.0192 0.0176 0.0222 0.0146 0.0191 0.0218 0.0203 0.0186 0.0243 0.0357 0.0117 0.0189 0.0139 0.0152 0.0167 0.0169 0.015 0.0209

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.4131 31.8186 31.481 41.2158 51.2764 43.4793 40.2147 47.0465 48.4861 50.4624
Translational Efficiency 2.501 3.3377 3.0317 1.5238 2.2636 3.1101 2.9715 2.1558 2.3776 2.5364

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments
Localization
Cell Percentages ER (12%)
Cell Cycle Regulation No
Subcompartmental Group ER-2

Dyn2

Dyn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dyn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available