Standard name | |
---|---|
Human Ortholog | |
Description | Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 1 | 1 | 1 | 7 | 1 | 3 | 6 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 5 | 1 | 3 | 4 |
Bud Neck | 0 | 0 | 1 | 2 | 1 | 3 | 5 | 8 | 6 | 8 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 1 | 3 | 10 | 13 | 19 | 13 | 9 | 27 | 11 | 18 | 0 | 1 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 12 | 7 | 15 | 12 | 14 | 14 | 5 | 3 | 5 | 6 | 42 | 57 | 84 | 29 | 21 | 33 | 0 | 0 | 1 | 1 | 2 | 3 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 4 | 1 | 3 | 3 | 0 | 1 | 7 | 2 | 7 | 13 |
Golgi | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 0 | 1 | 2 | 1 | 0 | 2 |
Mitochondria | 32 | 4 | 5 | 11 | 5 | 43 | 50 | 31 | 31 | 58 | 54 | 66 | 1 | 0 | 0 | 4 | 11 | 11 | 0 | 0 | 1 | 3 | 8 | 11 |
Nucleus | 171 | 177 | 369 | 213 | 343 | 319 | 420 | 423 | 295 | 346 | 243 | 300 | 391 | 354 | 411 | 271 | 340 | 276 | 171 | 160 | 327 | 366 | 505 | 418 |
Nuclear Periphery | 0 | 1 | 0 | 3 | 1 | 3 | 8 | 4 | 5 | 0 | 4 | 3 | 1 | 4 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 54 | 55 | 106 | 49 | 87 | 143 | 220 | 261 | 229 | 292 | 208 | 276 | 26 | 17 | 39 | 23 | 52 | 27 | 11 | 26 | 74 | 43 | 77 | 92 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 1 | 2 | 1 | 3 | 4 | 5 | 0 | 1 | 2 | 5 | 8 | 13 | 5 | 3 | 2 | 0 | 3 | 5 | 2 | 5 | 12 |
Vac/Vac Membrane | 0 | 2 | 0 | 3 | 1 | 1 | 1 | 5 | 1 | 1 | 0 | 1 | 3 | 2 | 4 | 2 | 4 | 6 | 3 | 3 | 14 | 16 | 31 | 36 |
Unique Cell Count | 191 | 192 | 422 | 241 | 386 | 398 | 570 | 608 | 443 | 537 | 372 | 499 | 446 | 432 | 524 | 316 | 397 | 340 | 190 | 203 | 444 | 444 | 648 | 608 |
Labelled Cell Count | 261 | 242 | 503 | 294 | 466 | 540 | 747 | 765 | 590 | 742 | 532 | 677 | 471 | 446 | 558 | 340 | 447 | 371 | 190 | 203 | 444 | 444 | 648 | 608 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 8.3 | 7.5 | 7.2 | 6.8 | 6.1 | 6.2 | 6.2 | 6.5 | 6.0 | 5.5 | 5.8 | 8.8 | 8.8 | 8.2 | 10.4 | 9.3 | 9.8 | 9.2 | 9.5 | 9.7 |
Std Deviation (1e-4) | 1.0 | 1.9 | 1.7 | 1.9 | 2.0 | 2.1 | 1.7 | 1.5 | 1.6 | 1.6 | 1.6 | 1.6 | 1.9 | 2.0 | 2.0 | 2.5 | 2.0 | 2.2 | 1.6 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.15 | -0.3 | -0.27 | -0.27 | -0.2 | -0.32 | -0.43 | -0.36 | 0.24 | 0.23 | 0.13 | 0.47 | 0.32 | 0.39 | 0.3 | 0.34 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 2.9 | 3.3 | 3.4 | 2.6 | 0 | 4.4 | 3.1 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.8 | 0.1 | -0.4 | -0.5 | -1.8 | -2.8 | -1.5 | -1.8 | 4.0 | 5.5 | 6.7 | 3.7 | 1.8 | 4.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.7 | 0 | 5.9 | 5.2 | 3.4 | 4.3 | 6.0 | 7.1 | 6.8 | 0 | 0 | 0 | 0 | 1.6 | 2.0 |
Nucleus | 0.4 | 0.6 | -2.8 | -5.3 | -6.7 | -7.3 | -8.1 | -7.4 | -9.3 | 0.1 | -2.2 | -3.6 | -0.7 | -0.8 | -2.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.4 | -0.9 | 3.4 | 4.5 | 5.9 | 8.0 | 9.1 | 8.9 | 9.3 | -7.9 | -8.8 | -7.5 | -6.3 | -4.4 | -6.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0126 | 5.3967 | 4.9951 | 4.5013 | 4.7416 | 4.7539 | 4.513 | 4.054 | 3.9506 | 3.7071 | 3.3201 | 3.9321 | 4.5222 | 4.3241 | 4.175 | 4.113 | 3.584 | 3.8007 |
Actin | 0.0246 | 0 | 0 | 0 | 0.0002 | 0 | 0.0602 | 0 | 0.0033 | 0.0059 | 0.0168 | 0.0003 | 0.0342 | 0 | 0.0323 | 0.0234 | 0 | 0.0076 |
Bud | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0005 | 0.0001 | 0.0001 |
Bud Neck | 0.0009 | 0 | 0 | 0 | 0.0014 | 0.0013 | 0.0071 | 0 | 0.0001 | 0.0015 | 0.0003 | 0.0005 | 0.0011 | 0 | 0.0015 | 0.0002 | 0.0003 | 0.0005 |
Bud Periphery | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0021 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0009 | 0.0006 | 0.0001 | 0.0001 |
Bud Site | 0.0003 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0095 | 0 | 0.0011 | 0.0009 | 0.0004 | 0.0001 | 0.0015 | 0 | 0.0014 | 0.0006 | 0.0001 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0009 | 0 | 0 | 0 | 0.0002 | 0 | 0.0223 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0003 | 0.0017 | 0.0005 | 0 |
Cytoplasmic Foci | 0.0012 | 0 | 0 | 0 | 0.0001 | 0 | 0.026 | 0 | 0.0077 | 0.0211 | 0.0002 | 0.0009 | 0.0129 | 0 | 0.0003 | 0.002 | 0.0001 | 0.0003 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0 | 0 | 0.0003 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0.0044 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0019 | 0.0023 | 0.0002 | 0.0003 |
Endosome | 0.0035 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0274 | 0 | 0.0025 | 0.0275 | 0.0004 | 0.0007 | 0.009 | 0 | 0.0011 | 0.0091 | 0.0005 | 0.0017 |
Golgi | 0.0014 | 0 | 0 | 0 | 0.0001 | 0 | 0.0158 | 0 | 0.002 | 0.0156 | 0.0012 | 0.0004 | 0.0041 | 0 | 0.0009 | 0.0021 | 0 | 0.0015 |
Lipid Particles | 0.0011 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0227 | 0 | 0.006 | 0.0204 | 0.0006 | 0.0085 | 0.0046 | 0 | 0.0004 | 0.0016 | 0.0002 | 0.0011 |
Mitochondria | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0403 | 0.0001 | 0.0045 | 0.0022 | 0.0198 | 0.0006 | 0.001 | 0.0001 | 0.0015 | 0.0026 | 0.0005 | 0.008 |
None | 0.0023 | 0 | 0 | 0 | 0.0002 | 0 | 0.0148 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0013 | 0 | 0.0003 | 0.0012 | 0.0003 | 0.0001 |
Nuclear Periphery | 0.0082 | 0.0001 | 0.0002 | 0.0002 | 0.0021 | 0.0002 | 0.0258 | 0.0001 | 0.0002 | 0.0006 | 0.0039 | 0.0034 | 0.0018 | 0.0002 | 0.0031 | 0.022 | 0.0062 | 0.0007 |
Nucleolus | 0.0233 | 0.0229 | 0.0238 | 0.009 | 0.1833 | 0.0937 | 0.0289 | 0.0202 | 0.0168 | 0.018 | 0.0911 | 0.0631 | 0.0292 | 0.0306 | 0.0154 | 0.0112 | 0.1969 | 0.0709 |
Nucleus | 0.9255 | 0.9768 | 0.9756 | 0.9906 | 0.785 | 0.903 | 0.6517 | 0.9794 | 0.9404 | 0.8737 | 0.8613 | 0.9184 | 0.8787 | 0.9654 | 0.9347 | 0.9086 | 0.789 | 0.9059 |
Peroxisomes | 0.0007 | 0 | 0 | 0 | 0.0001 | 0 | 0.015 | 0 | 0.0132 | 0.0023 | 0.0011 | 0.0001 | 0.0031 | 0 | 0.0002 | 0.0008 | 0 | 0.0002 |
Punctate Nuclear | 0.0024 | 0.0001 | 0.0002 | 0.0001 | 0.0259 | 0.0006 | 0.0147 | 0.0001 | 0.0011 | 0.0071 | 0.0011 | 0.0019 | 0.0144 | 0.0036 | 0.0016 | 0.008 | 0.0021 | 0.0005 |
Vacuole | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0.0053 | 0 | 0.0003 | 0.0011 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0007 | 0.0006 | 0.0016 | 0.0001 |
Vacuole Periphery | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0043 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0005 | 0.0009 | 0.0014 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.8697 | 18.1403 | 17.9478 | 15.6863 | 23.0213 | 15.2109 | 21.0164 | 19.3427 | 18.0395 | 22.0265 |
Translational Efficiency | 0.8026 | 0.9087 | 0.685 | 0.7012 | 0.8574 | 1.0427 | 0.673 | 0.7599 | 0.6839 | 0.7088 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|