Standard name
Human Ortholog
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.09 0.05 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0.05 0.07 0.09 0.07 0.11 0.12 0.16 0.14 0 0 0 0.1 0.1 0.09 0 0 0 0 0 0
Nucleus 0.16 0.16 0.13 0.09 0.11 0.06 0.08 0.07 0.06 0.07 0.1 0.09 0.11 0.1 0.1 0.16 0.18 0.18 0.09 0.12 0.09 0.07 0.16 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.92 0.91 0.91 0.91 0.87 0.9 0.86 0.84 0.85 0.83 0.86 0.85 0.87 0.82 0.84 0.81 0.81 0.85 0.81 0.79 0.76 0.84 0.7 0.71
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0 0.11 0.13 0.22 0.35 0.33 0.32 0.31 0.26 0.26 0.28 0.1 0.25 0.16 0 0 0 0.07 0 0.09 0 0.05 0.07
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 2 2 3 12 5 7 15 6 8 0 0 1 0 0 0 2 1 3 0 2 3
Bud Neck 3 1 10 7 20 16 37 22 24 39 13 12 4 5 6 1 1 1 0 1 1 0 1 2
Bud Site 1 0 1 4 6 17 21 18 20 21 18 18 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 3 6 13 9 12 17 7 10 4 7 6 19 16 17 19 3 0 0 0 0 3 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 1 4 1 0 0 0 0 0 0
Endosome 0 0 2 4 11 15 3 12 7 6 5 12 0 4 4 3 3 2 0 2 2 0 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Mitochondria 9 8 8 11 21 30 56 44 60 90 74 97 2 1 1 35 32 20 1 0 1 0 2 1
Nucleus 38 19 44 26 44 25 46 44 33 58 49 60 11 21 18 58 57 41 20 15 28 2 35 29
Nuclear Periphery 2 0 1 0 0 2 2 1 0 2 3 5 0 0 0 1 1 0 1 0 0 0 0 0
Nucleolus 217 110 299 258 358 390 526 541 453 650 401 570 85 180 157 293 252 193 188 99 247 30 154 152
Peroxisomes 2 0 2 2 6 11 19 14 10 15 8 9 1 0 1 4 3 4 0 0 0 0 0 0
SpindlePole 12 2 36 36 91 153 200 205 165 203 124 190 10 54 30 4 3 2 15 2 29 1 9 14
Vac/Vac Membrane 0 0 2 1 0 1 0 2 1 0 1 1 0 1 0 9 3 2 0 1 4 0 4 2
Unique Cell Count 237 121 330 285 413 435 612 644 532 779 469 671 98 219 187 363 311 226 234 126 326 36 220 214
Labelled Cell Count 288 143 408 357 573 672 935 925 787 1111 706 989 119 285 234 426 378 269 234 126 326 36 220 214


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 12.4 12.3 11.4 9.6 7.7 7.4 7.2 6.8 6.6 6.3 6.4 11.6 12.8 11.5 21.5 20.8 22.1 10.2 14.2 14.4
Std Deviation (1e-4) 2.7 3.6 3.5 4.7 3.5 2.0 2.7 2.5 2.0 2.0 2.0 1.8 3.6 5.1 4.8 8.9 10.0 9.4 2.7 6.1 5.1
Intensity Change (Log2) -0.11 -0.36 -0.68 -0.74 -0.77 -0.86 -0.9 -0.97 -0.93 -0.08 0.06 -0.1 0.8 0.76 0.84 -0.28 0.21 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -0.4 1.2 0.5 2.0 0.3 1.1 1.5 -0.2 -1.3 0 -0.5 0.1 -2.9 -2.6 0
Bud Site 0 0 3.3 3.0 2.7 3.2 2.6 3.2 2.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.1 1.3 1.2 1.8 0 0 0 0 0 4.5 4.4 3.0 3.6 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.1 2.6 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.0 1.9 2.8 3.9 2.9 4.7 4.9 6.1 5.8 0 0 0 3.9 4.1 3.4
Nucleus -1.6 -1.1 -3.6 -2.9 -3.3 -3.6 -3.1 -1.3 -2.1 -0.5 -1.3 -1.2 1.0 1.7 1.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 -1.7 -0.4 -2.1 -2.8 -2.3 -3.1 -2.2 -2.5 -1.1 -2.9 -2.3 -3.7 -3.5 -1.9
Peroxisomes 0 0 2.0 2.5 1.8 0 1.6 0 0 0 0 0 0 0 0
SpindlePole 0.7 4.0 7.7 7.4 7.2 6.8 5.6 5.4 6.2 -0.2 4.3 1.7 -5.5 -5.3 -4.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.4963 11.2397 10.3961 9.6299 9.6887 10.2906 8.8491 12.0808 10.8594 9.7598 9.4305 9.9447 7.4015 11.1948 10.0605 9.1863 7.8727 9.1672
Actin 0 0 0.0049 0.0084 0 0 0.026 0 0.0043 0.0002 0.0004 0.0003 0.0275 0 0.0002 0.0013 0.0084 0.0003
Bud 0 0 0 0.0002 0 0 0.0006 0.0006 0.0001 0.0003 0.0001 0 0.0004 0 0 0 0.0002 0
Bud Neck 0.0002 0.0001 0.0001 0.0449 0.0004 0.0001 0.0006 0.0001 0.0002 0.0005 0.0003 0.0001 0.0005 0.0001 0.0003 0.0003 0.0042 0.0002
Bud Periphery 0 0 0 0.0004 0.0001 0 0.0009 0.0004 0.0002 0.0005 0.0001 0.0001 0.0006 0 0 0.0001 0.0004 0
Bud Site 0.0002 0.0002 0.0001 0.0032 0.0001 0 0.0072 0.0002 0.0008 0.0012 0.0003 0.0001 0.0021 0.0001 0.0001 0.0002 0.0007 0.0001
Cell Periphery 0.0001 0 0.0003 0.0003 0.0001 0 0.0006 0.0001 0.0003 0.0006 0.0003 0.0001 0.0006 0.0002 0.0001 0.0001 0.0007 0.0001
Cytoplasm 0 0 0.0001 0.0001 0 0 0.0056 0.0001 0.0025 0.0007 0.0008 0.0001 0.0044 0 0 0.0001 0.0006 0
Cytoplasmic Foci 0.0013 0.0001 0.0014 0.0013 0.0004 0.0001 0.0108 0.0001 0.0092 0.0036 0.0147 0.0045 0.0366 0.0001 0.0036 0.0041 0.003 0.0018
Eisosomes 0 0 0 0.0002 0 0 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0 0 0.0002 0 0 0 0.0024 0 0.0006 0.0003 0.0004 0.0004 0.0031 0 0 0 0.0017 0
Endosome 0.0001 0 0.0013 0.0009 0 0 0.014 0 0.0053 0.0149 0.0024 0.0057 0.0164 0 0.0004 0.0013 0.0143 0.0007
Golgi 0.0001 0 0.001 0.0017 0 0 0.0081 0 0.0021 0.0013 0.0007 0.0026 0.0064 0 0.0004 0.0012 0.0032 0.0006
Lipid Particles 0.0027 0 0.0015 0.0018 0.0011 0.0004 0.0193 0.0001 0.0076 0.0072 0.0098 0.0086 0.0215 0.0001 0.0031 0.0101 0.0058 0.0021
Mitochondria 0.0002 0.0001 0.0002 0.0004 0.0013 0.0001 0.0168 0.0001 0.0084 0.0011 0.0053 0.0084 0.0188 0.0002 0.0003 0.0013 0.0048 0.0006
None 0 0 0.0002 0.0001 0 0 0.0034 0 0.0011 0.0012 0.0013 0.0002 0.0068 0 0.0001 0.0005 0.0008 0
Nuclear Periphery 0 0 0.0001 0 0.0005 0 0.0229 0 0.0011 0.0041 0.0068 0.0003 0.017 0 0 0.0001 0.0033 0
Nucleolus 0.9216 0.8615 0.8995 0.8208 0.8983 0.9142 0.7296 0.8769 0.8806 0.8608 0.8414 0.882 0.7291 0.8567 0.8918 0.8834 0.8661 0.9127
Nucleus 0.0706 0.1329 0.0868 0.1113 0.082 0.0836 0.0942 0.12 0.0673 0.0881 0.1023 0.0748 0.062 0.142 0.0908 0.0709 0.0731 0.0784
Peroxisomes 0.0002 0 0.0006 0.0025 0.0001 0 0.0028 0 0.0026 0.0011 0.001 0.0005 0.008 0 0.0033 0.0218 0.0008 0.0015
Punctate Nuclear 0.0024 0.0049 0.0008 0.0009 0.0148 0.0013 0.0279 0.0001 0.0018 0.0047 0.0081 0.0095 0.032 0.0001 0.0051 0.003 0.0044 0.0007
Vacuole 0.0003 0.0001 0.0006 0.0003 0.0002 0.0001 0.0027 0.0008 0.0016 0.0062 0.0012 0.0005 0.0027 0.0003 0.0002 0.0002 0.0021 0.0001
Vacuole Periphery 0 0 0.0001 0.0001 0.0004 0 0.0036 0.0002 0.0021 0.0018 0.0022 0.0012 0.0029 0.0001 0.0001 0.0001 0.0012 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.6158 35.7869 30.9813 30.7958 42.1722 20.0053 23.1804 23.6586 22.8402 32.812
Translational Efficiency 1.4826 1.5432 1.5417 1.19 1.0163 1.7999 1.5242 1.3122 1.1357 1.1607

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
Localization
Cell Percentages nucleolus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Utp6

Utp6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utp6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available