Standard name | |
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Human Ortholog | |
Description | Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 2 | 2 | 3 | 12 | 5 | 7 | 15 | 6 | 8 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 2 | 3 |
Bud Neck | 3 | 1 | 10 | 7 | 20 | 16 | 37 | 22 | 24 | 39 | 13 | 12 | 4 | 5 | 6 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 2 |
Bud Site | 1 | 0 | 1 | 4 | 6 | 17 | 21 | 18 | 20 | 21 | 18 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 3 | 3 | 6 | 13 | 9 | 12 | 17 | 7 | 10 | 4 | 7 | 6 | 19 | 16 | 17 | 19 | 3 | 0 | 0 | 0 | 0 | 3 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 2 | 4 | 11 | 15 | 3 | 12 | 7 | 6 | 5 | 12 | 0 | 4 | 4 | 3 | 3 | 2 | 0 | 2 | 2 | 0 | 3 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 9 | 8 | 8 | 11 | 21 | 30 | 56 | 44 | 60 | 90 | 74 | 97 | 2 | 1 | 1 | 35 | 32 | 20 | 1 | 0 | 1 | 0 | 2 | 1 |
Nucleus | 38 | 19 | 44 | 26 | 44 | 25 | 46 | 44 | 33 | 58 | 49 | 60 | 11 | 21 | 18 | 58 | 57 | 41 | 20 | 15 | 28 | 2 | 35 | 29 |
Nuclear Periphery | 2 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 2 | 3 | 5 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 217 | 110 | 299 | 258 | 358 | 390 | 526 | 541 | 453 | 650 | 401 | 570 | 85 | 180 | 157 | 293 | 252 | 193 | 188 | 99 | 247 | 30 | 154 | 152 |
Peroxisomes | 2 | 0 | 2 | 2 | 6 | 11 | 19 | 14 | 10 | 15 | 8 | 9 | 1 | 0 | 1 | 4 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 12 | 2 | 36 | 36 | 91 | 153 | 200 | 205 | 165 | 203 | 124 | 190 | 10 | 54 | 30 | 4 | 3 | 2 | 15 | 2 | 29 | 1 | 9 | 14 |
Vac/Vac Membrane | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 9 | 3 | 2 | 0 | 1 | 4 | 0 | 4 | 2 |
Unique Cell Count | 237 | 121 | 330 | 285 | 413 | 435 | 612 | 644 | 532 | 779 | 469 | 671 | 98 | 219 | 187 | 363 | 311 | 226 | 234 | 126 | 326 | 36 | 220 | 214 |
Labelled Cell Count | 288 | 143 | 408 | 357 | 573 | 672 | 935 | 925 | 787 | 1111 | 706 | 989 | 119 | 285 | 234 | 426 | 378 | 269 | 234 | 126 | 326 | 36 | 220 | 214 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.5 | 12.4 | 12.3 | 11.4 | 9.6 | 7.7 | 7.4 | 7.2 | 6.8 | 6.6 | 6.3 | 6.4 | 11.6 | 12.8 | 11.5 | 21.5 | 20.8 | 22.1 | 10.2 | 14.2 | 14.4 |
Std Deviation (1e-4) | 2.7 | 3.6 | 3.5 | 4.7 | 3.5 | 2.0 | 2.7 | 2.5 | 2.0 | 2.0 | 2.0 | 1.8 | 3.6 | 5.1 | 4.8 | 8.9 | 10.0 | 9.4 | 2.7 | 6.1 | 5.1 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.36 | -0.68 | -0.74 | -0.77 | -0.86 | -0.9 | -0.97 | -0.93 | -0.08 | 0.06 | -0.1 | 0.8 | 0.76 | 0.84 | -0.28 | 0.21 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -0.4 | 1.2 | 0.5 | 2.0 | 0.3 | 1.1 | 1.5 | -0.2 | -1.3 | 0 | -0.5 | 0.1 | -2.9 | -2.6 | 0 |
Bud Site | 0 | 0 | 3.3 | 3.0 | 2.7 | 3.2 | 2.6 | 3.2 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.1 | 1.3 | 1.2 | 1.8 | 0 | 0 | 0 | 0 | 0 | 4.5 | 4.4 | 3.0 | 3.6 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.1 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.0 | 1.9 | 2.8 | 3.9 | 2.9 | 4.7 | 4.9 | 6.1 | 5.8 | 0 | 0 | 0 | 3.9 | 4.1 | 3.4 |
Nucleus | -1.6 | -1.1 | -3.6 | -2.9 | -3.3 | -3.6 | -3.1 | -1.3 | -2.1 | -0.5 | -1.3 | -1.2 | 1.0 | 1.7 | 1.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | -1.7 | -0.4 | -2.1 | -2.8 | -2.3 | -3.1 | -2.2 | -2.5 | -1.1 | -2.9 | -2.3 | -3.7 | -3.5 | -1.9 |
Peroxisomes | 0 | 0 | 2.0 | 2.5 | 1.8 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.7 | 4.0 | 7.7 | 7.4 | 7.2 | 6.8 | 5.6 | 5.4 | 6.2 | -0.2 | 4.3 | 1.7 | -5.5 | -5.3 | -4.6 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.4963 | 11.2397 | 10.3961 | 9.6299 | 9.6887 | 10.2906 | 8.8491 | 12.0808 | 10.8594 | 9.7598 | 9.4305 | 9.9447 | 7.4015 | 11.1948 | 10.0605 | 9.1863 | 7.8727 | 9.1672 |
Actin | 0 | 0 | 0.0049 | 0.0084 | 0 | 0 | 0.026 | 0 | 0.0043 | 0.0002 | 0.0004 | 0.0003 | 0.0275 | 0 | 0.0002 | 0.0013 | 0.0084 | 0.0003 |
Bud | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0006 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 |
Bud Neck | 0.0002 | 0.0001 | 0.0001 | 0.0449 | 0.0004 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0042 | 0.0002 |
Bud Periphery | 0 | 0 | 0 | 0.0004 | 0.0001 | 0 | 0.0009 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0004 | 0 |
Bud Site | 0.0002 | 0.0002 | 0.0001 | 0.0032 | 0.0001 | 0 | 0.0072 | 0.0002 | 0.0008 | 0.0012 | 0.0003 | 0.0001 | 0.0021 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0001 |
Cytoplasm | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0056 | 0.0001 | 0.0025 | 0.0007 | 0.0008 | 0.0001 | 0.0044 | 0 | 0 | 0.0001 | 0.0006 | 0 |
Cytoplasmic Foci | 0.0013 | 0.0001 | 0.0014 | 0.0013 | 0.0004 | 0.0001 | 0.0108 | 0.0001 | 0.0092 | 0.0036 | 0.0147 | 0.0045 | 0.0366 | 0.0001 | 0.0036 | 0.0041 | 0.003 | 0.0018 |
Eisosomes | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0024 | 0 | 0.0006 | 0.0003 | 0.0004 | 0.0004 | 0.0031 | 0 | 0 | 0 | 0.0017 | 0 |
Endosome | 0.0001 | 0 | 0.0013 | 0.0009 | 0 | 0 | 0.014 | 0 | 0.0053 | 0.0149 | 0.0024 | 0.0057 | 0.0164 | 0 | 0.0004 | 0.0013 | 0.0143 | 0.0007 |
Golgi | 0.0001 | 0 | 0.001 | 0.0017 | 0 | 0 | 0.0081 | 0 | 0.0021 | 0.0013 | 0.0007 | 0.0026 | 0.0064 | 0 | 0.0004 | 0.0012 | 0.0032 | 0.0006 |
Lipid Particles | 0.0027 | 0 | 0.0015 | 0.0018 | 0.0011 | 0.0004 | 0.0193 | 0.0001 | 0.0076 | 0.0072 | 0.0098 | 0.0086 | 0.0215 | 0.0001 | 0.0031 | 0.0101 | 0.0058 | 0.0021 |
Mitochondria | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0013 | 0.0001 | 0.0168 | 0.0001 | 0.0084 | 0.0011 | 0.0053 | 0.0084 | 0.0188 | 0.0002 | 0.0003 | 0.0013 | 0.0048 | 0.0006 |
None | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0034 | 0 | 0.0011 | 0.0012 | 0.0013 | 0.0002 | 0.0068 | 0 | 0.0001 | 0.0005 | 0.0008 | 0 |
Nuclear Periphery | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0229 | 0 | 0.0011 | 0.0041 | 0.0068 | 0.0003 | 0.017 | 0 | 0 | 0.0001 | 0.0033 | 0 |
Nucleolus | 0.9216 | 0.8615 | 0.8995 | 0.8208 | 0.8983 | 0.9142 | 0.7296 | 0.8769 | 0.8806 | 0.8608 | 0.8414 | 0.882 | 0.7291 | 0.8567 | 0.8918 | 0.8834 | 0.8661 | 0.9127 |
Nucleus | 0.0706 | 0.1329 | 0.0868 | 0.1113 | 0.082 | 0.0836 | 0.0942 | 0.12 | 0.0673 | 0.0881 | 0.1023 | 0.0748 | 0.062 | 0.142 | 0.0908 | 0.0709 | 0.0731 | 0.0784 |
Peroxisomes | 0.0002 | 0 | 0.0006 | 0.0025 | 0.0001 | 0 | 0.0028 | 0 | 0.0026 | 0.0011 | 0.001 | 0.0005 | 0.008 | 0 | 0.0033 | 0.0218 | 0.0008 | 0.0015 |
Punctate Nuclear | 0.0024 | 0.0049 | 0.0008 | 0.0009 | 0.0148 | 0.0013 | 0.0279 | 0.0001 | 0.0018 | 0.0047 | 0.0081 | 0.0095 | 0.032 | 0.0001 | 0.0051 | 0.003 | 0.0044 | 0.0007 |
Vacuole | 0.0003 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0027 | 0.0008 | 0.0016 | 0.0062 | 0.0012 | 0.0005 | 0.0027 | 0.0003 | 0.0002 | 0.0002 | 0.0021 | 0.0001 |
Vacuole Periphery | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0036 | 0.0002 | 0.0021 | 0.0018 | 0.0022 | 0.0012 | 0.0029 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.6158 | 35.7869 | 30.9813 | 30.7958 | 42.1722 | 20.0053 | 23.1804 | 23.6586 | 22.8402 | 32.812 |
Translational Efficiency | 1.4826 | 1.5432 | 1.5417 | 1.19 | 1.0163 | 1.7999 | 1.5242 | 1.3122 | 1.1357 | 1.1607 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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