Standard name
Human Ortholog
Description Dual endo- and exopolyphosphatase with a role in phosphate metabolism; acts as both an endopolyphosphatase cleaving long chains of polyphosphate distributively to generate shorter polymer chains and as an exopolyphosphatase catalyzing the hydrolysis of terminal phosphate from polyphosphate; localizes to the vacuole, nucleus and cytosol; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.27 0.4 0.45 0.65 0.35 0.38 0.41 – 0.49 0.59 0.65 0.66 0.87 0.91 0.95 0.25 0.27 0.27 0 0 0.05 0.06 0.11 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0 0 0 0 0.05 0 – 0 0.05 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.16 0.14 0.1 0 0.14 0.22 0.25 – 0.16 0.13 0.06 0.07 0.06 0 0 0.26 0.21 0.22 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.55 0.7 0.63 0.55 0.71 0.7 0.6 – 0.61 0.59 0.4 0.48 0.18 0.14 0.05 0.66 0.66 0.68 0.91 0.89 0.86 0.82 0.79 0.72
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 0 0 0 0 1 – 2 2 1 0 0 0 0 0 0 0 0 1 2 0 2 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 1 – 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 33 94 82 108 98 111 167 – 152 196 208 185 117 202 248 49 59 59 2 7 10 4 25 26
Endoplasmic Reticulum 0 0 0 0 0 2 0 – 1 0 9 2 1 1 0 3 10 9 0 0 0 0 0 0
Endosome 0 6 1 3 9 1 3 – 2 1 0 0 0 2 2 12 3 11 3 10 3 0 4 12
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 2 0 1 0 1 0 0 1
Mitochondria 32 5 1 1 3 14 12 – 11 16 31 14 0 0 2 7 4 0 1 2 3 2 7 7
Nucleus 20 32 18 5 40 64 103 – 49 44 20 20 8 1 1 51 46 49 1 3 2 1 3 4
Nuclear Periphery 0 1 1 0 0 3 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 1 1 0 – 0 1 0 0 0 0 0 2 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 1 – 0 0 0 1 0 0 0 4 0 0 0 1 0 0 1 2
Vac/Vac Membrane 68 164 115 92 199 205 242 – 189 196 129 134 24 32 13 131 143 148 134 228 177 69 181 182
Unique Cell Count 123 235 183 167 280 291 404 310 334 322 279 135 222 261 199 218 218 149 257 207 84 231 255
Labelled Cell Count 153 302 218 210 351 402 530 407 456 401 358 150 239 266 262 267 276 149 257 207 84 231 255


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.2 6.1 6.3 7.6 6.5 6.6 – 6.0 6.1 5.2 5.3 5.5 5.5 5.2 8.0 8.3 7.6 5.4 5.4 6.0
Std Deviation (1e-4) 0.7 0.7 1.4 1.7 2.1 1.6 1.7 – 1.5 2.2 1.2 1.0 1.0 1.5 1.5 1.7 1.9 1.5 0.8 1.2 1.7
Intensity Change (Log2) – – – 0.04 0.32 0.1 0.12 – -0.03 0.01 -0.24 -0.2 -0.15 -0.14 -0.24 0.39 0.45 0.33 -0.16 -0.17 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.7 -2.1 -1.4 -0.8 – 0.9 3.0 4.3 4.6 7.6 10.1 11.9 -4.2 -3.7 -3.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 3.0 0 2.6
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.6 0 – 0 2.6 4.0 2.7 0 0 0 0 0 0
Nucleus -2.6 1.4 3.4 4.3 – 1.9 1.1 -1.5 -1.0 -1.3 -4.4 -4.8 4.0 3.1 3.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -1.5 1.9 1.7 -0.7 – -0.4 -0.9 -4.9 -3.1 -8.0 -10.1 -13.2 0.6 0.6 1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3645 0.8425 0.684 0.4677 0.0502 0.7717 -0.3332 1.2714 0.7674 0.5094 0.6093 0.8907 -0.2521 1.0852 0.7957 0.8719 0.3929 0.5861
Actin 0.0117 0.0001 0.004 0.0044 0.0011 0 0.0004 0.0013 0.0015 0.0002 0.0046 0.0015 0.0037 0 0 0.0098 0 0.0009
Bud 0.0006 0.0004 0.0007 0.0007 0.0028 0.0004 0.0012 0.0203 0.0012 0.0114 0.0171 0.0054 0.0007 0.0006 0.0004 0.0004 0.0002 0.0003
Bud Neck 0.0046 0.001 0.0022 0.0006 0.0079 0.0018 0.0014 0.0011 0.0023 0.0031 0.0026 0.0031 0.0017 0.0011 0.0016 0.0032 0.0011 0.0019
Bud Periphery 0.001 0.0007 0.0013 0.0022 0.003 0.0012 0.0021 0.0085 0.0017 0.0095 0.0025 0.0067 0.0014 0.0008 0.0008 0.0006 0.0007 0.0011
Bud Site 0.0094 0.0014 0.0089 0.001 0.0827 0.0008 0.0019 0.0131 0.0016 0.02 0.0071 0.0023 0.0017 0.0035 0.0018 0.0027 0.0005 0.0008
Cell Periphery 0.0018 0.0018 0.0029 0.001 0.0025 0.0025 0.0032 0.0021 0.0028 0.0031 0.0016 0.0038 0.001 0.0015 0.0018 0.0009 0.0027 0.0018
Cytoplasm 0.0132 0.0273 0.0142 0.0019 0.0034 0.0174 0.0273 0.0358 0.0213 0.0052 0.0169 0.0278 0.021 0.0325 0.0244 0.0077 0.0026 0.0134
Cytoplasmic Foci 0.0312 0.016 0.0219 0.0229 0.0225 0.0106 0.0432 0.0341 0.034 0.0501 0.0481 0.0318 0.0207 0.0119 0.0092 0.0101 0.0021 0.0088
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0002 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0045 0.0032 0.0048 0.0011 0.0008 0.0041 0.0081 0.0098 0.0032 0.001 0.0044 0.0028 0.0056 0.0092 0.0046 0.0032 0.0012 0.0027
Endosome 0.2735 0.1732 0.2555 0.1884 0.1251 0.2094 0.2698 0.1698 0.213 0.2857 0.1822 0.1552 0.2896 0.1434 0.2284 0.2004 0.1777 0.1847
Golgi 0.018 0.0033 0.011 0.0273 0.0336 0.0052 0.0105 0.0076 0.0067 0.0055 0.0137 0.0052 0.0066 0.0019 0.005 0.0077 0.0015 0.0028
Lipid Particles 0.0385 0.0151 0.0228 0.0498 0.0619 0.031 0.0624 0.0142 0.0174 0.0445 0.0525 0.0251 0.0152 0.0112 0.0113 0.0164 0.0029 0.0061
Mitochondria 0.0302 0.0174 0.0243 0.0759 0.0183 0.0036 0.0108 0.0255 0.0147 0.0092 0.0063 0.0288 0.0114 0.0027 0.0138 0.0073 0.0006 0.003
None 0.0158 0.0217 0.0369 0.0003 0.0003 0.0075 0.0921 0.041 0.0623 0.0435 0.0082 0.0195 0.0173 0.0275 0.0111 0.0006 0.0002 0.0016
Nuclear Periphery 0.0064 0.0043 0.0069 0.0017 0.0021 0.003 0.0056 0.0129 0.0056 0.0013 0.0056 0.0097 0.0121 0.0123 0.0121 0.0063 0.0036 0.0126
Nucleolus 0.0112 0.0021 0.006 0.0012 0.0059 0.0046 0.0044 0.0061 0.0066 0.0097 0.0039 0.0098 0.0025 0.0042 0.0061 0.0026 0.0033 0.0033
Nucleus 0.0096 0.0039 0.0058 0.0013 0.0037 0.011 0.0069 0.0208 0.0253 0.0036 0.0057 0.0221 0.0083 0.0141 0.0113 0.006 0.0256 0.0117
Peroxisomes 0.0131 0.0022 0.0026 0.0227 0.0099 0.0009 0.0147 0.0032 0.0048 0.0051 0.0422 0.0094 0.004 0.0007 0.0015 0.0029 0.0003 0.0022
Punctate Nuclear 0.0009 0.0004 0.0028 0.0005 0.0007 0.0004 0.0047 0.0056 0.0007 0.0013 0.0017 0.0026 0.0082 0.001 0.0007 0.0048 0.0001 0.0023
Vacuole 0.404 0.5919 0.4673 0.4883 0.5529 0.5664 0.3712 0.4893 0.4809 0.4282 0.5154 0.5317 0.4528 0.6303 0.5412 0.5855 0.6697 0.6286
Vacuole Periphery 0.1002 0.1126 0.0971 0.1069 0.059 0.1181 0.0579 0.0778 0.0924 0.0588 0.0575 0.0959 0.1143 0.0897 0.113 0.1206 0.1033 0.1093

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.3931 56.0164 49.157 58.4262 50.8662 58.2711 73.8758 82.7696 78.3417 67.4112
Translational Efficiency 0.677 0.6243 0.7572 0.63 0.6131 0.6942 0.6118 0.4709 0.5146 0.5818

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dual endo- and exopolyphosphatase with a role in phosphate metabolism; acts as both an endopolyphosphatase cleaving long chains of polyphosphate distributively to generate shorter polymer chains and as an exopolyphosphatase catalyzing the hydrolysis of terminal phosphate from polyphosphate; localizes to the vacuole, nucleus and cytosol; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
Localization
Cell Percentages vacuole (79%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ppn1

Ppn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ppn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available