Standard name | |
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Human Ortholog | |
Description | Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 22 | 66 | 21 | 5 | 4 | 3 | 2 | 10 | 9 | 3 | 6 | 8 | 65 | 73 | 35 | 31 | 30 | 20 | 1 | 11 | 9 | 6 | 14 | 11 |
Bud | 3 | 8 | 7 | 4 | 2 | 20 | 15 | 24 | 13 | 14 | 18 | 21 | 7 | 7 | 5 | 1 | 5 | 3 | 1 | 1 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 1 | 0 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 36 | 17 | 23 | 20 | 27 | 18 | 19 | 14 | 21 | 18 | 13 | 17 | 17 | 25 | 43 | 25 | 28 | 0 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 21 | 88 | 32 | 3 | 9 | 15 | 15 | 22 | 14 | 7 | 8 | 10 | 44 | 40 | 40 | 22 | 10 | 8 | 1 | 10 | 2 | 4 | 8 | 8 |
Endosome | 89 | 85 | 175 | 107 | 140 | 52 | 53 | 61 | 16 | 16 | 15 | 41 | 175 | 174 | 136 | 134 | 61 | 62 | 194 | 258 | 203 | 106 | 167 | 116 |
Golgi | 64 | 136 | 117 | 61 | 38 | 25 | 29 | 22 | 14 | 11 | 12 | 11 | 376 | 399 | 494 | 151 | 145 | 99 | 82 | 170 | 174 | 101 | 255 | 232 |
Mitochondria | 28 | 76 | 47 | 84 | 70 | 272 | 341 | 325 | 263 | 337 | 210 | 303 | 24 | 24 | 26 | 45 | 30 | 26 | 6 | 11 | 6 | 14 | 16 | 13 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 2 | 2 | 4 | 0 | 5 | 3 | 5 | 4 | 3 | 1 | 2 | 3 | 4 | 7 | 7 | 6 | 4 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 5 | 2 | 3 | 6 | 7 | 14 | 14 | 17 | 9 | 18 | 6 | 11 | 5 | 5 | 5 | 10 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 4 | 4 | 3 | 1 | 1 | 3 | 2 | 1 | 2 | 12 | 3 | 5 | 0 | 1 | 0 | 0 | 1 | 2 | 9 | 4 | 5 | 3 |
SpindlePole | 3 | 0 | 27 | 6 | 4 | 7 | 7 | 14 | 1 | 5 | 6 | 9 | 26 | 26 | 7 | 10 | 7 | 7 | 3 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 70 | 56 | 20 | 18 | 22 | 31 | 27 | 14 | 19 | 27 | 10 | 19 | 130 | 134 | 143 | 51 | 49 | 45 | 14 | 19 | 0 | 3 | 8 | 6 |
Unique Cell Count | 249 | 405 | 343 | 248 | 253 | 381 | 436 | 430 | 310 | 397 | 256 | 373 | 711 | 725 | 768 | 393 | 284 | 220 | 310 | 494 | 411 | 244 | 486 | 399 |
Labelled Cell Count | 329 | 558 | 472 | 327 | 320 | 477 | 528 | 537 | 382 | 470 | 315 | 463 | 875 | 908 | 924 | 508 | 372 | 309 | 310 | 494 | 411 | 244 | 486 | 399 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 9.9 | 9.2 | 8.7 | 8.1 | 7.0 | 6.8 | 6.7 | 6.7 | 6.4 | 6.8 | 6.7 | 11.3 | 11.3 | 11.6 | 9.4 | 11.1 | 11.3 | 9.0 | 10.3 | 10.8 |
Std Deviation (1e-4) | 1.5 | 1.5 | 1.4 | 1.8 | 1.7 | 1.6 | 1.4 | 1.2 | 1.3 | 1.3 | 1.6 | 1.3 | 1.6 | 1.6 | 1.6 | 1.8 | 2.2 | 2.7 | 1.3 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.19 | -0.4 | -0.44 | -0.44 | -0.46 | -0.52 | -0.43 | -0.45 | 0.3 | 0.3 | 0.34 | 0.03 | 0.28 | 0.31 | -0.02 | 0.17 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.4 | -2.7 | -4.0 | -4.6 | -2.7 | -2.0 | -4.1 | -2.2 | -2.7 | 1.7 | 2.1 | -1.1 | 0.9 | 2.0 | 1.3 |
Bud | 0 | 0 | 2.3 | 1.2 | 2.5 | 1.6 | 1.2 | 3.0 | 2.5 | 0 | 0 | 0 | 0 | -0.3 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.1 | 1.5 | 1.2 | -0.6 | -0.4 | -0.3 | 0.2 | 1.1 | -1.0 | -2.2 | -2.3 | -1.4 | 3.0 | 1.9 | 3.3 |
Endoplasmic Reticulum | -4.1 | -2.8 | -2.9 | -3.4 | -2.3 | -2.4 | -4.6 | -3.0 | -3.8 | -1.8 | -2.3 | -2.6 | -1.9 | -2.9 | -2.6 |
Endosome | -1.9 | 1.0 | -10.8 | -11.8 | -11.0 | -12.9 | -14.6 | -11.7 | -11.7 | -8.5 | -8.8 | -11.4 | -4.6 | -7.6 | -5.4 |
Golgi | -2.5 | -5.3 | -9.3 | -9.7 | -10.4 | -9.4 | -11.2 | -8.7 | -10.9 | 5.7 | 6.4 | 9.4 | 1.2 | 4.3 | 2.6 |
Mitochondria | 5.8 | 4.2 | 15.6 | 17.9 | 17.1 | 18.2 | 19.3 | 16.7 | 18.1 | -6.3 | -6.4 | -6.4 | -0.9 | -1.2 | -0.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2.5 | 2.2 | 2.7 | 1.9 | 3.0 | 0 | 2.0 | 0 | 0 | 0 | 1.7 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -2.8 | -3.4 | -3.8 | -4.2 | -2.8 | -4.8 | -4.4 | -2.9 | -3.3 | -2.9 | -3.0 | -6.2 | -3.3 | -3.0 | -2.3 |
Vacuole | 0.7 | 1.4 | 1.2 | 0.2 | -1.7 | 0.2 | 0.5 | -1.1 | -0.4 | 5.4 | 5.5 | 5.6 | 3.3 | 4.5 | 5.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0859 | 6.4829 | 6.2517 | 5.678 | 5.5421 | 6.0762 | 8.0263 | 8.7839 | 8.3565 | 7.0546 | 7.6209 | 8.2274 | 8.8583 | 10.0322 | 9.2855 | 9.3886 | 9.0531 | 9.6688 |
Actin | 0.0154 | 0.0008 | 0.0127 | 0.0003 | 0.0023 | 0.0014 | 0.0236 | 0.0008 | 0.0032 | 0.0001 | 0.0003 | 0.0005 | 0.0049 | 0.0017 | 0.0025 | 0.0002 | 0.0004 | 0.0019 |
Bud | 0.0006 | 0.0005 | 0.0003 | 0.012 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0011 | 0.0003 | 0.0008 | 0.0003 | 0.0004 | 0.0003 | 0.0006 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Bud Periphery | 0.0007 | 0.0004 | 0.0002 | 0.0053 | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0017 | 0.0006 | 0.0007 | 0.0016 | 0.0047 | 0.0001 | 0.0005 | 0.0002 | 0.0007 | 0 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.012 | 0.0201 | 0.0073 | 0.0036 | 0.0041 | 0.0081 | 0.0043 | 0.0126 | 0.0111 | 0.0013 | 0.0023 | 0.0021 | 0.0034 | 0.0064 | 0.0026 | 0.0016 | 0.0016 | 0.0021 |
Cytoplasmic Foci | 0.1097 | 0.1276 | 0.1198 | 0.0929 | 0.0664 | 0.1012 | 0.1256 | 0.1638 | 0.1394 | 0.0797 | 0.1319 | 0.1172 | 0.1123 | 0.1343 | 0.1263 | 0.104 | 0.1178 | 0.1142 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0094 | 0.0088 | 0.0018 | 0.0011 | 0.0019 | 0.0058 | 0.0045 | 0.0026 | 0.0022 | 0.001 | 0.0011 | 0.0031 | 0.002 | 0.0039 | 0.0035 | 0.0007 | 0.0009 | 0.0021 |
Endosome | 0.519 | 0.5888 | 0.5159 | 0.6224 | 0.5443 | 0.4488 | 0.4799 | 0.4582 | 0.4049 | 0.5053 | 0.4265 | 0.417 | 0.4607 | 0.4903 | 0.4428 | 0.4927 | 0.4595 | 0.3998 |
Golgi | 0.2106 | 0.2051 | 0.271 | 0.2094 | 0.2276 | 0.3486 | 0.2693 | 0.2929 | 0.3814 | 0.3869 | 0.382 | 0.3897 | 0.3055 | 0.2901 | 0.372 | 0.3736 | 0.3515 | 0.4119 |
Lipid Particles | 0.0489 | 0.0206 | 0.0286 | 0.0124 | 0.0389 | 0.0574 | 0.0657 | 0.0514 | 0.0278 | 0.0126 | 0.0401 | 0.0434 | 0.0806 | 0.0572 | 0.0286 | 0.0174 | 0.0598 | 0.0518 |
Mitochondria | 0.0142 | 0.0076 | 0.0059 | 0.0053 | 0.0375 | 0.0083 | 0.0061 | 0.0027 | 0.0108 | 0.0036 | 0.004 | 0.0083 | 0.004 | 0.0023 | 0.0023 | 0.002 | 0.0007 | 0.0045 |
None | 0.0029 | 0.0008 | 0.0002 | 0.0006 | 0.0003 | 0.0016 | 0.0003 | 0.0001 | 0.0024 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0018 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0158 | 0.0014 | 0.0021 | 0.0007 | 0.0008 | 0.0032 | 0.004 | 0.0003 | 0.0012 | 0.0002 | 0.0008 | 0.0005 | 0.0006 | 0.0005 | 0.0024 | 0.0002 | 0.0002 | 0.0003 |
Nucleolus | 0.0005 | 0.0001 | 0.0003 | 0.0015 | 0.0006 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0037 | 0.0003 | 0.0005 | 0.0087 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Peroxisomes | 0.0121 | 0.0033 | 0.0073 | 0.0024 | 0.0027 | 0.0049 | 0.0074 | 0.0064 | 0.0068 | 0.0026 | 0.0053 | 0.0122 | 0.0136 | 0.0045 | 0.0108 | 0.0023 | 0.0038 | 0.0057 |
Punctate Nuclear | 0.0027 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0004 | 0.0007 | 0.0002 | 0.0015 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0116 | 0.0087 | 0.0185 | 0.0112 | 0.0448 | 0.0045 | 0.0041 | 0.0054 | 0.0027 | 0.0031 | 0.0029 | 0.0024 | 0.0076 | 0.0032 | 0.0025 | 0.0024 | 0.0014 | 0.0022 |
Vacuole Periphery | 0.007 | 0.0039 | 0.0056 | 0.0076 | 0.0189 | 0.0049 | 0.0025 | 0.0019 | 0.0021 | 0.0033 | 0.0022 | 0.003 | 0.0037 | 0.0026 | 0.0027 | 0.0027 | 0.002 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.1587 | 27.4858 | 36.943 | 49.6959 | 29.3343 | 30.7298 | 47.8474 | 55.0322 | 50.1556 | 55.8558 |
Translational Efficiency | 0.5594 | 0.817 | 0.7635 | 0.4815 | 0.6434 | 1.084 | 0.6359 | 0.498 | 0.6506 | 0.5359 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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