Standard name
Human Ortholog
Description E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0.06 0.05 – 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.09 0 0 0.05 0 0 0 – 0.05 0 0.07 0.14 0.13 0.06 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.13 0.29 0.22 0.16 0.32 0.33 0.31 0.41 – 0.39 0.43 0.07 0 0.05 0.06 0 0.16 0 0 0 0 0 0
Nucleus 0.56 0.43 0.37 0.15 0.11 0.13 0.11 0.14 0.13 – 0.16 0.14 0.26 0.17 0.13 0.38 0.36 0.34 0.27 0.33 0.23 0.33 0.19 0.17
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.39 0.63 0.47 0.78 0.76 0.67 0.72 0.74 0.68 – 0.71 0.69 0.75 0.75 0.76 0.56 0.56 0.65 0.57 0.52 0.44 0.48 0.55 0.58
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.33 0.34 0.23 0.24 0.24 0.21 – 0.24 0.2 0.07 0.2 0.18 0 0 0 0 0 0.07 0.05 0.07 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0.05 0.06 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 1 0 1 0 1 0
Bud 0 1 7 4 5 9 4 11 14 – 1 9 0 3 3 0 1 1 6 5 7 5 6 8
Bud Neck 0 1 1 9 9 4 1 10 4 – 2 8 3 4 2 0 0 0 0 0 2 1 2 2
Bud Site 1 0 5 9 6 13 21 27 12 – 14 19 1 1 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 10 3 27 6 8 15 14 13 7 – 12 12 18 31 36 22 19 30 0 0 3 1 0 0
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 1 – 0 0 0 0 1 4 4 4 0 0 2 0 1 0
Endosome 1 1 4 0 10 2 4 3 1 – 4 1 6 2 7 5 8 4 9 5 23 5 17 19
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 1 1 1 0 1 0 1 3 3 1
Mitochondria 1 22 92 48 39 97 130 141 109 – 92 121 17 3 13 24 11 66 1 1 9 2 2 4
Nucleus 123 72 115 33 28 39 45 63 34 – 37 39 68 39 36 149 114 139 72 60 79 84 60 65
Nuclear Periphery 14 7 8 0 2 3 5 6 2 – 2 6 2 0 1 11 10 8 5 0 6 0 2 0
Nucleolus 86 105 148 175 191 201 283 334 181 – 165 197 196 170 211 219 179 267 151 95 149 122 178 226
Peroxisomes 0 0 0 0 0 0 3 1 3 – 1 0 0 1 1 0 2 0 0 0 0 0 1 0
SpindlePole 2 4 31 74 84 70 93 110 56 – 56 56 18 46 50 17 14 13 5 5 24 11 23 33
Vac/Vac Membrane 7 4 7 1 2 1 2 4 1 – 0 2 4 2 1 11 19 10 8 4 18 15 20 24
Unique Cell Count 221 167 315 223 250 299 392 453 266 233 284 260 227 277 390 320 411 266 183 340 257 324 393
Labelled Cell Count 247 220 446 360 384 454 607 723 425 386 470 333 302 363 463 382 543 266 183 340 257 324 393


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 7.5 5.2 5.5 5.8 5.5 5.3 5.0 4.6 – 4.5 4.4 7.6 7.9 7.2 11.1 12.5 10.9 7.6 8.4 9.0
Std Deviation (1e-4) 2.4 2.6 1.4 2.3 1.5 1.9 2.1 1.5 1.4 – 1.7 2.0 2.9 2.6 2.4 3.8 4.4 3.5 2.3 2.5 2.9
Intensity Change (Log2) – – – 0.08 0.17 0.09 0.04 -0.05 -0.17 – -0.21 -0.22 0.56 0.62 0.48 1.11 1.27 1.08 0.55 0.71 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 -0.2 0.6 0 0.2 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 1.8 0 2.0 2.6 3.0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -2.8 -2.6 -1.7 -2.8 -3.5 -3.0 – -1.5 -2.2 -0.7 1.9 1.7 -1.5 -1.3 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 2.1 0 0 0 0 – 0 0 0 0 1.1 0 1.1 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria -2.0 -3.8 0.9 1.1 0.6 0 – 0 0 -6.9 -8.4 -7.8 -8.2 -8.8 -4.3
Nucleus -5.6 -6.9 -6.7 -7.9 -7.3 -6.5 – -5.3 -6.4 -2.7 -4.9 -6.5 0.5 -0.2 -0.8
Nuclear Periphery 0 0 -1.4 -1.2 -1.2 0 – 0 0 0 0 0 0.2 0.4 -0.5
Nucleolus 7.3 7.1 5.1 6.8 7.5 0 – 0 0 6.9 6.5 7.3 2.4 2.3 4.9
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 6.7 7.0 4.5 4.8 5.1 0 – 0 0 -1.2 3.4 2.9 -2.9 -2.7 -3.7
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0.5 2.4 0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0466 12.5861 10.8619 9.9179 9.6491 11.041 4.1655 6.9717 6.2322 4.8317 6.1118 5.5327 11.7122 14.1871 12.4253 11.6614 11.7071 12.0773
Actin 0.0344 0 0.0081 0 0.004 0.0002 0.0057 0 0.0004 0.0004 0.0013 0.0023 0.0066 0 0 0 0 0
Bud 0.0002 0 0.0004 0.0016 0.0009 0 0.0007 0.0001 0.0002 0.0001 0.0005 0.0001 0.0003 0 0.0003 0.0001 0.0001 0
Bud Neck 0.0011 0.0002 0.0007 0.0049 0.0263 0.0003 0.0012 0.0002 0.0008 0.0007 0.0003 0.0002 0.0003 0.0003 0.0005 0.0011 0.0005 0.0002
Bud Periphery 0.0004 0.0001 0.0009 0.0013 0.0019 0.0001 0.0006 0.0001 0.0001 0.0002 0.0007 0.0003 0.0003 0.0001 0.0006 0.0001 0.0002 0
Bud Site 0.0018 0.0002 0.0045 0.0012 0.0032 0.0001 0.0108 0.0002 0.0034 0.0002 0.0004 0.0002 0.0024 0.0001 0.0002 0.0002 0.0001 0.0001
Cell Periphery 0.0005 0.0004 0.0007 0.0019 0.0004 0.0002 0.0007 0.0006 0.0003 0.0003 0.0002 0.001 0.0004 0.0003 0.0007 0.0004 0.0003 0.0001
Cytoplasm 0.0014 0 0.0018 0 0.0016 0 0.0022 0 0.0008 0.0011 0.002 0.0004 0.0001 0 0 0 0 0
Cytoplasmic Foci 0.0058 0.0001 0.0015 0.0005 0.0016 0.0028 0.0314 0.0028 0.0085 0.0038 0.0018 0.0049 0.0018 0.0001 0.0002 0.003 0.0003 0.0004
Eisosomes 0.0003 0 0.0002 0 0 0 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0008 0 0.0012 0 0.0018 0.0001 0.0019 0 0.0002 0.0003 0.0051 0.0011 0.0001 0 0 0 0 0
Endosome 0.0037 0 0.0125 0.0001 0.0242 0.0006 0.0141 0.0004 0.0025 0.0046 0.057 0.013 0.0005 0 0.0001 0.0001 0.0001 0
Golgi 0.0031 0 0.0033 0 0.0034 0.0005 0.0041 0.0001 0.0006 0.0047 0.0033 0.0051 0.0004 0 0 0.0002 0 0
Lipid Particles 0.0147 0.0009 0.0062 0.0008 0.0023 0.0044 0.03 0.002 0.0061 0.0228 0.0028 0.0342 0.0041 0.0004 0.0007 0.0087 0.0012 0.0012
Mitochondria 0.0045 0.0002 0.0227 0.0002 0.0032 0.0091 0.0035 0.0009 0.0007 0.007 0.0034 0.0067 0.0011 0.0001 0.0001 0.0014 0.0007 0
None 0.003 0 0.0004 0 0.0003 0.0001 0.0011 0 0.0005 0.0002 0.0011 0.0006 0.0004 0 0 0.0001 0 0
Nuclear Periphery 0.0019 0.0002 0.0027 0 0.0038 0.0002 0.0042 0.0001 0.0005 0.0046 0.0255 0.0318 0.0002 0.0002 0.0036 0.0002 0.0002 0
Nucleolus 0.7635 0.8261 0.8404 0.8469 0.7701 0.8539 0.7659 0.8412 0.8549 0.7887 0.8011 0.7912 0.8248 0.8468 0.8852 0.8889 0.8573 0.8696
Nucleus 0.1313 0.1694 0.0834 0.1315 0.1257 0.1253 0.0997 0.1391 0.1154 0.1529 0.0721 0.0916 0.1517 0.1463 0.1041 0.09 0.133 0.1269
Peroxisomes 0.017 0 0.0008 0.0002 0.0005 0.0002 0.0032 0.0002 0.0006 0.0033 0.0002 0.0014 0.0009 0 0 0.0006 0.0002 0
Punctate Nuclear 0.0043 0.0004 0.0006 0.0022 0.0069 0.0008 0.0133 0.0003 0.001 0.0018 0.0016 0.0051 0.0019 0.0045 0.0006 0.0017 0.0018 0.0012
Vacuole 0.0033 0.0011 0.0044 0.0057 0.0138 0.0005 0.0038 0.0081 0.0016 0.0016 0.0088 0.0033 0.0013 0.0007 0.0022 0.0013 0.0018 0.0001
Vacuole Periphery 0.0027 0.0005 0.0025 0.0009 0.0039 0.0006 0.0017 0.0035 0.001 0.0006 0.0107 0.0054 0.0006 0.0001 0.0008 0.0018 0.0023 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.6488 35.2026 47.3178 63.9698 44.4194 42.7309 50.2628 64.0386 62.1618 65.033
Translational Efficiency 0.4647 0.4397 0.492 0.3728 0.4065 0.4734 0.4122 0.2845 0.3238 0.3333

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
Localization
Cell Percentages nucleolus (47%), nucleus (10%), mixed (16%)
Cell Cycle Regulation Yes
nucleolus - T/G1 (1.1e-08)
nucleolus - S/G2 (4.8e-10)
nucleus - MA (1.5e-12)
Subcompartmental Group N/A

Tom1

Tom1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tom1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available