Standard name
Human Ortholog
Description Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.3 0.64 0.99 0.99 0.89 0.86 0.9 0.79 0.88 0.63 0.73 0.55 0.63 0.88 0.7 0.54 0.54 0.12 0.14 0.34 0.47 0.82 0.84
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.1 0 0 0 0 0 0
Endosome 0 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.12 0.1 0.05 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0 0 0 0 0.08 0.15 0.09 0.26 0.14 0.51 0.3 0 0 0 0 0 0 0 0.07 0.13 0.06 0 0
Nucleus 0.38 0.35 0.21 0 0 0 0 0 0 0 0 0 0.3 0.16 0 0.09 0.1 0.07 0.3 0.25 0.09 0.09 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0.05 0.09 0 0
Vac/Vac Membrane 0.15 0.29 0.07 0 0 0 0 0 0 0 0 0 0.09 0.11 0.07 0.17 0.26 0.26 0.25 0.24 0.25 0.16 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 2 1 0 0 3 7 0 0 3
Bud 1 0 0 0 0 4 6 4 4 0 7 4 0 0 0 0 1 1 6 12 3 0 1 2
Bud Neck 1 3 15 5 13 8 15 14 7 19 1 7 1 0 3 4 4 9 6 6 5 3 0 0
Bud Site 2 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0
Cell Periphery 0 1 2 2 3 3 3 1 2 0 1 2 0 2 2 3 3 2 0 0 1 0 0 0
Cytoplasm 35 108 135 295 360 374 422 493 334 351 274 313 71 119 245 223 211 214 38 75 115 49 102 155
Endoplasmic Reticulum 4 2 10 0 1 0 0 1 0 1 1 0 2 7 0 26 24 40 0 2 3 0 0 1
Endosome 7 24 1 0 0 0 1 1 2 0 0 1 7 10 12 13 47 39 16 28 5 2 0 2
Golgi 0 3 1 0 0 0 0 0 0 0 0 0 0 0 1 2 0 2 0 3 0 0 0 0
Mitochondria 54 13 0 3 2 32 75 48 110 54 222 129 1 2 0 11 11 5 14 39 45 6 2 5
Nucleus 73 127 44 0 0 7 2 10 6 1 5 5 39 31 8 28 39 29 96 135 29 9 2 1
Nuclear Periphery 2 1 0 0 0 0 0 0 0 1 2 0 0 0 0 0 1 0 3 3 0 0 0 0
Nucleolus 2 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 25 2 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 7 9 1 1 0 6 9 10 12 5 12 12 2 4 0 8 14 13 32 61 15 9 2 5
Vac/Vac Membrane 29 104 14 1 0 3 8 1 8 8 2 5 12 21 19 53 103 101 80 129 84 16 6 5
Unique Cell Count 191 361 212 298 363 420 488 549 421 399 432 429 130 189 278 319 392 393 323 545 341 105 126 186
Labelled Cell Count 217 397 224 308 380 438 541 583 486 441 529 478 135 196 290 373 459 456 323 545 341 105 126 186


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.9 5.4 4.7 4.9 4.2 4.3 4.5 3.6 3.9 3.3 3.6 6.2 6.2 5.6 5.8 6.2 5.7 4.5 5.6 5.3
Std Deviation (1e-4) 0.5 0.8 0.6 1.2 1.0 1.3 1.4 1.4 1.0 1.1 1.0 0.9 1.1 1.7 1.4 0.9 1.2 0.9 0.6 1.8 1.1
Intensity Change (Log2) -0.2 -0.15 -0.37 -0.34 -0.25 -0.59 -0.48 -0.73 -0.6 0.2 0.19 0.04 0.11 0.21 0.09 -0.26 0.05 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 -2.7 -3.7 -3.5 -3.5 -4.1 -2.9
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10.8 11.9 7.6 6.9 8.5 4.2 7.1 -0.1 2.4 -1.7 -0.1 6.4 1.5 -2.3 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 -0.5 0 1.5 0.7 2.3
Endosome 0 0 0 0 0 0 0 0 0 0 2.9 2.6 2.5 5.0 4.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -8.2 -9.0 -8.3 -10.0 -9.1 -8.5 -9.2 -8.8 -8.8 1.9 -1.1 -6.4 -3.9 -3.7 -4.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0
Vacuole -4.1 -5.0 -4.3 -3.5 0 -3.0 -2.9 -4.7 -3.8 0.9 1.6 0.1 3.4 5.8 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4003 1.6271 1.6189 1.1536 1.2816 1.4746 0.8047 0.6362 0.5018 0.3426 0.7775 0.66 1.3026 0.4884 0.4911 1.5475 0.6702 0.4197
Actin 0.0059 0.0006 0.0005 0.0003 0.0003 0.0008 0.0136 0.0046 0.0034 0.0058 0.006 0.0062 0.096 0.001 0.0187 0.0193 0.0395 0.0005
Bud 0.0015 0.0012 0.0059 0.001 0.0006 0.0028 0.0006 0.0007 0.0003 0.0036 0.0005 0.0003 0.0004 0.0116 0.0012 0.0052 0.0003 0.0002
Bud Neck 0.0021 0.0012 0.0014 0.0011 0.0021 0.0081 0.0038 0.0016 0.002 0.0019 0.0234 0.0154 0.0011 0.0016 0.0027 0.0038 0.0018 0.0053
Bud Periphery 0.0004 0.0006 0.0018 0.0003 0.0003 0.0013 0.0011 0.0011 0.0004 0.0103 0.0011 0.0003 0.0003 0.0013 0.0006 0.0061 0.0004 0.0001
Bud Site 0.0026 0.0055 0.0053 0.0011 0.0008 0.0005 0.0039 0.0062 0.0027 0.0033 0.0029 0.0005 0.0014 0.0087 0.0158 0.0105 0.0008 0.0004
Cell Periphery 0.0001 0.0001 0.0001 0 0 0.0001 0.0003 0.0002 0.0001 0.0002 0.0003 0.0001 0.0002 0.0003 0.0004 0.0006 0.0001 0.0001
Cytoplasm 0.0947 0.1164 0.0561 0.0423 0.1135 0.0707 0.1353 0.1634 0.1617 0.126 0.1251 0.15 0.2248 0.2869 0.3011 0.2492 0.3597 0.346
Cytoplasmic Foci 0.0058 0.0057 0.005 0.0009 0.0078 0.0054 0.0159 0.0067 0.0143 0.0169 0.046 0.0072 0.0254 0.0194 0.0309 0.0212 0.0335 0.0256
Eisosomes 0.0001 0 0.0001 0 0 0 0.0005 0.0002 0.0001 0.0001 0.0002 0.0001 0.0006 0.0001 0.0001 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0046 0.0032 0.0013 0.0003 0.0028 0.0017 0.0057 0.0041 0.004 0.0023 0.0062 0.0029 0.0099 0.0096 0.0065 0.011 0.0086 0.0057
Endosome 0.0072 0.0071 0.0025 0.0003 0.005 0.0031 0.0215 0.0048 0.0081 0.0193 0.0421 0.0112 0.017 0.0055 0.0109 0.0575 0.0194 0.0086
Golgi 0.0011 0.0002 0.0001 0 0.0001 0.0004 0.0048 0.0009 0.0012 0.0108 0.0099 0.0032 0.0048 0.0003 0.0034 0.0213 0.0151 0.0002
Lipid Particles 0.0007 0.0004 0.0003 0 0.0003 0.0039 0.0074 0.0009 0.0033 0.0288 0.0159 0.0052 0.0043 0.0009 0.0053 0.034 0.0353 0.0006
Mitochondria 0.0017 0.0009 0.0005 0.0003 0.0005 0.0008 0.0091 0.0013 0.0016 0.0146 0.0205 0.0014 0.0016 0.0007 0.002 0.0243 0.1182 0.0003
None 0.1039 0.1692 0.2106 0.1571 0.2573 0.0971 0.2702 0.4218 0.2906 0.3491 0.2836 0.1839 0.284 0.3201 0.2474 0.209 0.1785 0.218
Nuclear Periphery 0.0214 0.0207 0.0149 0.0066 0.018 0.0112 0.0255 0.0123 0.0183 0.0106 0.0334 0.0136 0.0259 0.0208 0.0144 0.0284 0.015 0.0083
Nucleolus 0.011 0.0115 0.0109 0.008 0.0095 0.0177 0.0077 0.0063 0.0058 0.0054 0.0054 0.0124 0.0044 0.004 0.0049 0.003 0.0006 0.0041
Nucleus 0.6151 0.5394 0.5094 0.6109 0.4055 0.6163 0.3814 0.309 0.4169 0.244 0.223 0.4813 0.1917 0.252 0.2896 0.2417 0.0556 0.2921
Peroxisomes 0.0012 0.0005 0.0002 0.0001 0.0002 0.0051 0.0051 0.0009 0.0085 0.0138 0.0161 0.0019 0.0121 0.0003 0.0044 0.0074 0.0445 0.0001
Punctate Nuclear 0.116 0.1111 0.172 0.1693 0.1735 0.1515 0.0797 0.05 0.054 0.1284 0.133 0.1001 0.0908 0.0512 0.034 0.0228 0.0697 0.0797
Vacuole 0.0024 0.0036 0.0008 0.0002 0.0017 0.0012 0.0051 0.0028 0.0022 0.0036 0.0042 0.0019 0.0027 0.0033 0.005 0.0165 0.0019 0.0037
Vacuole Periphery 0.0005 0.0007 0.0002 0.0001 0.0004 0.0003 0.0018 0.0004 0.0006 0.0012 0.0012 0.0006 0.0009 0.0004 0.0007 0.0071 0.001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.3534 24.6594 22.0518 31.5822 23.8435 19.7937 25.4126 29.7729 26.9156 33.6933
Translational Efficiency 1.5009 1.71 1.8626 1.0211 1.9722 2.5311 1.5228 1.634 1.886 1.8279

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (33%), cytoplasm (10%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Stp1

Stp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available