Standard name | |
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Human Ortholog | |
Description | Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 3 | 7 | 0 | 0 | 3 |
Bud | 1 | 0 | 0 | 0 | 0 | 4 | 6 | 4 | 4 | 0 | 7 | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 12 | 3 | 0 | 1 | 2 |
Bud Neck | 1 | 3 | 15 | 5 | 13 | 8 | 15 | 14 | 7 | 19 | 1 | 7 | 1 | 0 | 3 | 4 | 4 | 9 | 6 | 6 | 5 | 3 | 0 | 0 |
Bud Site | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 2 | 3 | 3 | 3 | 1 | 2 | 0 | 1 | 2 | 0 | 2 | 2 | 3 | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 35 | 108 | 135 | 295 | 360 | 374 | 422 | 493 | 334 | 351 | 274 | 313 | 71 | 119 | 245 | 223 | 211 | 214 | 38 | 75 | 115 | 49 | 102 | 155 |
Endoplasmic Reticulum | 4 | 2 | 10 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 7 | 0 | 26 | 24 | 40 | 0 | 2 | 3 | 0 | 0 | 1 |
Endosome | 7 | 24 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 7 | 10 | 12 | 13 | 47 | 39 | 16 | 28 | 5 | 2 | 0 | 2 |
Golgi | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 |
Mitochondria | 54 | 13 | 0 | 3 | 2 | 32 | 75 | 48 | 110 | 54 | 222 | 129 | 1 | 2 | 0 | 11 | 11 | 5 | 14 | 39 | 45 | 6 | 2 | 5 |
Nucleus | 73 | 127 | 44 | 0 | 0 | 7 | 2 | 10 | 6 | 1 | 5 | 5 | 39 | 31 | 8 | 28 | 39 | 29 | 96 | 135 | 29 | 9 | 2 | 1 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 25 | 2 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 9 | 1 | 1 | 0 | 6 | 9 | 10 | 12 | 5 | 12 | 12 | 2 | 4 | 0 | 8 | 14 | 13 | 32 | 61 | 15 | 9 | 2 | 5 |
Vac/Vac Membrane | 29 | 104 | 14 | 1 | 0 | 3 | 8 | 1 | 8 | 8 | 2 | 5 | 12 | 21 | 19 | 53 | 103 | 101 | 80 | 129 | 84 | 16 | 6 | 5 |
Unique Cell Count | 191 | 361 | 212 | 298 | 363 | 420 | 488 | 549 | 421 | 399 | 432 | 429 | 130 | 189 | 278 | 319 | 392 | 393 | 323 | 545 | 341 | 105 | 126 | 186 |
Labelled Cell Count | 217 | 397 | 224 | 308 | 380 | 438 | 541 | 583 | 486 | 441 | 529 | 478 | 135 | 196 | 290 | 373 | 459 | 456 | 323 | 545 | 341 | 105 | 126 | 186 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.9 | 5.4 | 4.7 | 4.9 | 4.2 | 4.3 | 4.5 | 3.6 | 3.9 | 3.3 | 3.6 | 6.2 | 6.2 | 5.6 | 5.8 | 6.2 | 5.7 | 4.5 | 5.6 | 5.3 |
Std Deviation (1e-4) | 0.5 | 0.8 | 0.6 | 1.2 | 1.0 | 1.3 | 1.4 | 1.4 | 1.0 | 1.1 | 1.0 | 0.9 | 1.1 | 1.7 | 1.4 | 0.9 | 1.2 | 0.9 | 0.6 | 1.8 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.15 | -0.37 | -0.34 | -0.25 | -0.59 | -0.48 | -0.73 | -0.6 | 0.2 | 0.19 | 0.04 | 0.11 | 0.21 | 0.09 | -0.26 | 0.05 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.7 | -3.7 | -3.5 | -3.5 | -4.1 | -2.9 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10.8 | 11.9 | 7.6 | 6.9 | 8.5 | 4.2 | 7.1 | -0.1 | 2.4 | -1.7 | -0.1 | 6.4 | 1.5 | -2.3 | -2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.5 | 0 | 1.5 | 0.7 | 2.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.6 | 2.5 | 5.0 | 4.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -8.2 | -9.0 | -8.3 | -10.0 | -9.1 | -8.5 | -9.2 | -8.8 | -8.8 | 1.9 | -1.1 | -6.4 | -3.9 | -3.7 | -4.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 |
Vacuole | -4.1 | -5.0 | -4.3 | -3.5 | 0 | -3.0 | -2.9 | -4.7 | -3.8 | 0.9 | 1.6 | 0.1 | 3.4 | 5.8 | 5.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4003 | 1.6271 | 1.6189 | 1.1536 | 1.2816 | 1.4746 | 0.8047 | 0.6362 | 0.5018 | 0.3426 | 0.7775 | 0.66 | 1.3026 | 0.4884 | 0.4911 | 1.5475 | 0.6702 | 0.4197 |
Actin | 0.0059 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0008 | 0.0136 | 0.0046 | 0.0034 | 0.0058 | 0.006 | 0.0062 | 0.096 | 0.001 | 0.0187 | 0.0193 | 0.0395 | 0.0005 |
Bud | 0.0015 | 0.0012 | 0.0059 | 0.001 | 0.0006 | 0.0028 | 0.0006 | 0.0007 | 0.0003 | 0.0036 | 0.0005 | 0.0003 | 0.0004 | 0.0116 | 0.0012 | 0.0052 | 0.0003 | 0.0002 |
Bud Neck | 0.0021 | 0.0012 | 0.0014 | 0.0011 | 0.0021 | 0.0081 | 0.0038 | 0.0016 | 0.002 | 0.0019 | 0.0234 | 0.0154 | 0.0011 | 0.0016 | 0.0027 | 0.0038 | 0.0018 | 0.0053 |
Bud Periphery | 0.0004 | 0.0006 | 0.0018 | 0.0003 | 0.0003 | 0.0013 | 0.0011 | 0.0011 | 0.0004 | 0.0103 | 0.0011 | 0.0003 | 0.0003 | 0.0013 | 0.0006 | 0.0061 | 0.0004 | 0.0001 |
Bud Site | 0.0026 | 0.0055 | 0.0053 | 0.0011 | 0.0008 | 0.0005 | 0.0039 | 0.0062 | 0.0027 | 0.0033 | 0.0029 | 0.0005 | 0.0014 | 0.0087 | 0.0158 | 0.0105 | 0.0008 | 0.0004 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0.0001 | 0.0001 |
Cytoplasm | 0.0947 | 0.1164 | 0.0561 | 0.0423 | 0.1135 | 0.0707 | 0.1353 | 0.1634 | 0.1617 | 0.126 | 0.1251 | 0.15 | 0.2248 | 0.2869 | 0.3011 | 0.2492 | 0.3597 | 0.346 |
Cytoplasmic Foci | 0.0058 | 0.0057 | 0.005 | 0.0009 | 0.0078 | 0.0054 | 0.0159 | 0.0067 | 0.0143 | 0.0169 | 0.046 | 0.0072 | 0.0254 | 0.0194 | 0.0309 | 0.0212 | 0.0335 | 0.0256 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0046 | 0.0032 | 0.0013 | 0.0003 | 0.0028 | 0.0017 | 0.0057 | 0.0041 | 0.004 | 0.0023 | 0.0062 | 0.0029 | 0.0099 | 0.0096 | 0.0065 | 0.011 | 0.0086 | 0.0057 |
Endosome | 0.0072 | 0.0071 | 0.0025 | 0.0003 | 0.005 | 0.0031 | 0.0215 | 0.0048 | 0.0081 | 0.0193 | 0.0421 | 0.0112 | 0.017 | 0.0055 | 0.0109 | 0.0575 | 0.0194 | 0.0086 |
Golgi | 0.0011 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0048 | 0.0009 | 0.0012 | 0.0108 | 0.0099 | 0.0032 | 0.0048 | 0.0003 | 0.0034 | 0.0213 | 0.0151 | 0.0002 |
Lipid Particles | 0.0007 | 0.0004 | 0.0003 | 0 | 0.0003 | 0.0039 | 0.0074 | 0.0009 | 0.0033 | 0.0288 | 0.0159 | 0.0052 | 0.0043 | 0.0009 | 0.0053 | 0.034 | 0.0353 | 0.0006 |
Mitochondria | 0.0017 | 0.0009 | 0.0005 | 0.0003 | 0.0005 | 0.0008 | 0.0091 | 0.0013 | 0.0016 | 0.0146 | 0.0205 | 0.0014 | 0.0016 | 0.0007 | 0.002 | 0.0243 | 0.1182 | 0.0003 |
None | 0.1039 | 0.1692 | 0.2106 | 0.1571 | 0.2573 | 0.0971 | 0.2702 | 0.4218 | 0.2906 | 0.3491 | 0.2836 | 0.1839 | 0.284 | 0.3201 | 0.2474 | 0.209 | 0.1785 | 0.218 |
Nuclear Periphery | 0.0214 | 0.0207 | 0.0149 | 0.0066 | 0.018 | 0.0112 | 0.0255 | 0.0123 | 0.0183 | 0.0106 | 0.0334 | 0.0136 | 0.0259 | 0.0208 | 0.0144 | 0.0284 | 0.015 | 0.0083 |
Nucleolus | 0.011 | 0.0115 | 0.0109 | 0.008 | 0.0095 | 0.0177 | 0.0077 | 0.0063 | 0.0058 | 0.0054 | 0.0054 | 0.0124 | 0.0044 | 0.004 | 0.0049 | 0.003 | 0.0006 | 0.0041 |
Nucleus | 0.6151 | 0.5394 | 0.5094 | 0.6109 | 0.4055 | 0.6163 | 0.3814 | 0.309 | 0.4169 | 0.244 | 0.223 | 0.4813 | 0.1917 | 0.252 | 0.2896 | 0.2417 | 0.0556 | 0.2921 |
Peroxisomes | 0.0012 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0051 | 0.0051 | 0.0009 | 0.0085 | 0.0138 | 0.0161 | 0.0019 | 0.0121 | 0.0003 | 0.0044 | 0.0074 | 0.0445 | 0.0001 |
Punctate Nuclear | 0.116 | 0.1111 | 0.172 | 0.1693 | 0.1735 | 0.1515 | 0.0797 | 0.05 | 0.054 | 0.1284 | 0.133 | 0.1001 | 0.0908 | 0.0512 | 0.034 | 0.0228 | 0.0697 | 0.0797 |
Vacuole | 0.0024 | 0.0036 | 0.0008 | 0.0002 | 0.0017 | 0.0012 | 0.0051 | 0.0028 | 0.0022 | 0.0036 | 0.0042 | 0.0019 | 0.0027 | 0.0033 | 0.005 | 0.0165 | 0.0019 | 0.0037 |
Vacuole Periphery | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0018 | 0.0004 | 0.0006 | 0.0012 | 0.0012 | 0.0006 | 0.0009 | 0.0004 | 0.0007 | 0.0071 | 0.001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.3534 | 24.6594 | 22.0518 | 31.5822 | 23.8435 | 19.7937 | 25.4126 | 29.7729 | 26.9156 | 33.6933 |
Translational Efficiency | 1.5009 | 1.71 | 1.8626 | 1.0211 | 1.9722 | 2.5311 | 1.5228 | 1.634 | 1.886 | 1.8279 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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