Standard name
Human Ortholog
Description Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.9 0.75 0.21 0.21 0.29 0.32 0.27 0.22 0.25 0.2 0.23 0.59 0.6 0.43 0.44 0.51 0.49 0.58 0.65 0.63 0.46 0.53 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.09 0.4 0.41 0.45 0.4 0.52 0.6 0.57 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.07 0.13 0.51 0.31 0.18 0.19 0.21 0.28 0.23 0.27 0.25 0.12 0.22 0.25 0.05 0 0 0.29 0.26 0.18 0.41 0.3 0.18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.37 0.13 0.28 0.29 0.51 0.35 0.31 0.27 0.3 0.21 0.21 0.21 0.46 0.49 0.51 0.66 0.64 0.6 0 0 0.09 0 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 1
Bud 0 0 0 0 0 3 1 8 4 9 3 1 0 0 0 0 0 1 4 1 2 12 13 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 3 6
Bud Site 0 0 0 0 0 0 2 1 1 2 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 1 1 0 1 0 1 0 5 2 1 0 0 0 0 0 0
Cytoplasm 160 205 158 18 39 68 93 79 59 90 37 52 261 344 289 196 176 148 143 151 140 159 263 349
Endoplasmic Reticulum 0 2 1 0 0 1 0 1 0 0 0 2 4 3 1 12 8 17 0 0 2 1 2 5
Endosome 4 0 2 0 3 2 3 8 4 1 0 0 14 0 29 3 3 5 4 3 5 3 9 21
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 25 15 9 1 0 0 0 0 2
Mitochondria 6 5 1 7 17 96 120 135 107 184 110 131 3 0 0 2 1 1 1 2 1 2 3 3
Nucleus 9 16 27 44 58 42 56 64 74 80 50 56 54 128 166 24 12 7 72 60 39 142 148 103
Nuclear Periphery 0 0 1 0 0 0 1 2 1 0 0 2 0 0 0 0 0 0 1 0 1 1 1 7
Nucleolus 0 0 1 0 1 2 0 1 0 1 1 1 0 0 1 2 3 2 0 0 0 1 2 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 4 10 19
Vac/Vac Membrane 81 29 59 25 95 83 89 79 79 75 38 49 204 278 344 292 220 184 9 7 19 15 33 33
Unique Cell Count 218 229 211 87 185 238 290 298 265 353 182 228 442 571 670 442 343 305 246 235 222 349 499 579
Labelled Cell Count 260 257 250 95 213 297 366 378 330 443 241 295 540 754 833 561 441 375 246 235 222 349 499 579


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 8.8 8.5 8.0 7.0 5.7 5.7 5.5 5.4 5.1 4.8 4.7 10.4 10.4 9.7 17.9 17.2 16.6 11.9 11.4 11.4
Std Deviation (1e-4) 1.3 1.6 1.3 1.7 1.5 1.2 1.2 1.1 1.4 1.4 1.0 1.0 1.8 1.5 2.0 3.8 2.9 3.8 1.7 1.9 1.7
Intensity Change (Log2) – – – -0.09 -0.29 -0.57 -0.57 -0.63 -0.67 -0.73 -0.84 -0.87 0.29 0.29 0.18 1.08 1.02 0.96 0.49 0.42 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -8.6 -10.7 -9.8 -9.5 -10.8 -11.5 -11.4 -10.8 -10.9 -3.9 -3.8 -8.0 -7.3 -5.5 -6.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Endosome 0 0 0 0 0 0 0 0 0 1.7 0 2.3 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.5 3.1 0
Mitochondria 0 4.2 10.2 10.6 11.3 10.3 12.6 0 0 0 0 0 0 0 0
Nucleus 7.0 4.5 1.4 1.9 2.5 4.0 2.9 3.7 3.1 -0.2 3.0 3.7 -3.3 -4.2 -4.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 4.8 1.6 0.7 -0.4 0.4 -1.8 -1.6 -1.6 4.4 5.2 5.9 9.1 8.3 7.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7702 6.2175 6.0582 5.5958 5.6837 5.9261 5.4877 6.9446 6.0432 5.53 5.1611 5.9352 4.3565 5.0414 4.7738 5.1994 5.1465 5.1734
Actin 0.0147 0.0006 0.0059 0.0013 0.0128 0.0042 0.0306 0.0051 0.0008 0.0103 0.0071 0.0026 0.0444 0.0004 0.0009 0.0043 0.0175 0.0013
Bud 0.0004 0.0004 0.0003 0.0004 0.0003 0.0002 0.0016 0.0005 0.0013 0.0002 0.0032 0.0002 0.0007 0.0006 0.0002 0.0002 0.0046 0.0002
Bud Neck 0.0028 0.0006 0.0009 0.0015 0.0015 0.0031 0.0017 0.0007 0.004 0.0012 0.0023 0.0026 0.0022 0.0018 0.0006 0.0029 0.0023 0.0017
Bud Periphery 0.0007 0.0001 0.0002 0.0001 0.0001 0.0001 0.0011 0.0002 0.0018 0.0001 0.0034 0.0002 0.0006 0.0001 0.0001 0.0001 0.0096 0.0001
Bud Site 0.0009 0.0012 0.0008 0.0007 0.0004 0.0002 0.002 0.0033 0.0059 0.001 0.0273 0.0002 0.0045 0.0177 0.0005 0.0003 0.021 0.0001
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0002 0.0001 0.0011 0 0.0004 0 0.0001 0.0001 0 0 0.0004 0
Cytoplasm 0.3479 0.4733 0.4539 0.4828 0.4034 0.4357 0.4044 0.6694 0.4787 0.5478 0.7008 0.5738 0.354 0.6287 0.5355 0.4949 0.3797 0.561
Cytoplasmic Foci 0.0119 0.0071 0.0035 0.0063 0.0043 0.0047 0.0284 0.0032 0.0036 0.0116 0.0255 0.0029 0.0129 0.0017 0.0141 0.0146 0.0086 0.0018
Eisosomes 0.0001 0 0.0001 0 0 0.0001 0.0002 0 0 0.0001 0.0001 0 0.0004 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0042 0.0023 0.0046 0.0026 0.0076 0.0023 0.0058 0.002 0.0021 0.001 0.0029 0.0035 0.014 0.0021 0.0017 0.009 0.0041 0.0034
Endosome 0.0183 0.0062 0.0048 0.0033 0.0164 0.0053 0.027 0.0015 0.0086 0.0072 0.0425 0.0053 0.0142 0.002 0.0149 0.0161 0.0248 0.0028
Golgi 0.0035 0.0003 0.0003 0.0002 0.0008 0.0007 0.0151 0.0003 0.0018 0.0134 0.0269 0.0031 0.0025 0.0001 0.0016 0.0012 0.0032 0.0001
Lipid Particles 0.0042 0.0001 0.0004 0.0001 0.0005 0.0009 0.0171 0.0001 0.004 0.0103 0.011 0.0076 0.0096 0 0.0029 0.0014 0.0056 0.0001
Mitochondria 0.0063 0.0008 0.0056 0.0006 0.0012 0.0039 0.0041 0.0007 0.001 0.0021 0.022 0.0023 0.0035 0.0003 0.0122 0.0006 0.037 0.0005
None 0.0172 0.0187 0.0185 0.0256 0.0226 0.016 0.0092 0.003 0.0013 0.0013 0.0014 0.0023 0.0271 0.0039 0.0071 0.0129 0.0033 0.0022
Nuclear Periphery 0.0161 0.0144 0.0152 0.0093 0.0375 0.0113 0.0199 0.0076 0.0093 0.0069 0.0062 0.0133 0.0392 0.0091 0.0082 0.0165 0.0291 0.0169
Nucleolus 0.0011 0.0008 0.0007 0.0006 0.0012 0.001 0.0015 0.0003 0.001 0.0002 0.0014 0.0006 0.002 0.0003 0.0004 0.0003 0.002 0.0003
Nucleus 0.5221 0.456 0.4691 0.4471 0.4691 0.491 0.3802 0.2968 0.4606 0.37 0.0972 0.3604 0.4305 0.3273 0.3876 0.4073 0.4181 0.4016
Peroxisomes 0.0055 0.0011 0.0003 0.0004 0.0004 0.0031 0.0124 0.0005 0.001 0.0114 0.0112 0.0007 0.0049 0.0001 0.0021 0.0009 0.0028 0.0001
Punctate Nuclear 0.0159 0.0127 0.0123 0.0164 0.016 0.0144 0.0329 0.0038 0.0022 0.0032 0.0016 0.0166 0.0288 0.0024 0.0079 0.0145 0.0184 0.0041
Vacuole 0.0045 0.0029 0.0019 0.0006 0.003 0.0015 0.0037 0.001 0.0092 0.0006 0.0048 0.0013 0.0024 0.0013 0.0013 0.0015 0.003 0.0011
Vacuole Periphery 0.0014 0.0005 0.0004 0.0002 0.001 0.0003 0.001 0.0002 0.0006 0.0002 0.0011 0.0005 0.0015 0.0002 0.0002 0.0004 0.0047 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.7243 28.6598 32.491 34.0838 33.4849 21.3616 27.7705 27.4357 27.6499 31.4855
Translational Efficiency 2.111 2.1329 1.6329 1.2582 1.1904 2.0358 1.5532 1.1741 1.082 1.1758

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (60%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rmt2

Rmt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rmt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available