Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.71 0.9 0.93 0.97 0.94 0.87 0.79 0.74 0.69 0.68 0.73 0.85 0.92 0.98 0.98 0.98 0.98 0.95 0.63 0.83 0.79 0.83 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.15 0.14 0.14 0.15 0.12 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0.08 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.17 0 0.05 0 0 0 0 0 0.05 0 0 0.07 0.06 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 1 1 5 6 13 13 14 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 2 0 1 1 1 0 0 2 1 5 1 0 0 1 0 0 0 0 4 5 2 6 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 3 1 0 6 11 15 9 20 8 11 0 4 2 0 0 0 0 0 0 0 0 0
Cytoplasm 202 82 243 200 257 201 223 243 207 249 125 188 143 172 172 135 108 90 189 74 215 122 147 196
Endoplasmic Reticulum 4 3 6 2 8 7 18 45 40 51 27 30 4 2 1 4 5 1 1 2 9 1 2 3
Endosome 0 4 5 1 0 0 1 1 0 1 1 2 8 4 1 0 0 0 0 1 2 1 2 1
Golgi 1 0 3 0 1 0 4 5 13 22 8 10 0 1 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 1 0 0 2 1 3 2 17 15 11 10 0 2 0 4 1 0 0 3 0 2 0 1
Nucleus 0 7 5 0 0 1 0 0 3 2 2 5 5 0 0 0 0 0 1 13 3 11 2 4
Nuclear Periphery 0 1 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0
Nucleolus 0 3 1 1 0 0 1 1 0 1 1 0 3 0 0 0 0 0 0 4 1 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 1 1 1 0 1 0 1 0 0 0 0 1 6 2 4 1
Vac/Vac Membrane 0 20 11 10 5 3 3 10 7 19 7 6 12 12 2 4 1 2 3 5 6 4 3 3
Unique Cell Count 207 115 269 214 266 214 255 307 278 361 183 259 168 187 176 138 110 92 199 118 260 155 177 221
Labelled Cell Count 210 123 277 217 275 221 267 329 306 396 209 278 176 198 180 147 115 94 199 118 260 155 177 221


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 101.4 47.2 81.4 75.6 61.0 45.4 38.1 33.9 29.2 30.1 25.6 26.9 71.5 71.0 65.0 81.1 105.5 114.8 71.8 75.5 72.0
Std Deviation (1e-4) 20.6 33.5 32.4 30.3 23.3 17.1 14.1 10.6 11.5 10.9 12.3 9.9 37.8 37.7 34.6 25.6 31.5 43.7 41.8 48.3 39.7
Intensity Change (Log2) – – – -0.11 -0.42 -0.84 -1.1 -1.26 -1.48 -1.44 -1.67 -1.6 -0.19 -0.2 -0.32 -0.01 0.37 0.5 -0.18 -0.11 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 3.1 4.4 3.9 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 2.3 2.6 1.7 2.9 0 2.2 0 0 0 0 0 0
Cytoplasm 1.2 2.9 1.4 -1.1 -3.7 -4.9 -6.4 -5.9 -5.3 -1.7 0.6 3.0 2.8 2.7 2.3
Endoplasmic Reticulum 0 0.6 0.7 2.6 5.2 5.1 5.1 5.0 4.3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 2.5 3.2 0 2.0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.1 3.4 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 -1.5 -1.7 -2.1 -0.5 -1.0 0.7 -0.1 -1.2 1.4 1.1 -1.8 -0.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 99.7089 121.0629 106.0613 106.5576 102.6441 105.9818 116.9865 138.1296 127.8264 133.985 100.0976 115.952 102.5116 129.1866 119.2302 107.6 104.895 115.4989
Actin 0.0057 0.0024 0.0053 0.0005 0.0014 0.0046 0.0019 0.0003 0.0012 0.0002 0.0075 0.0019 0.0006 0.0004 0.0041 0.0224 0.0061 0.0013
Bud 0.0013 0.0006 0.0014 0.0017 0.0006 0.0023 0.001 0.0007 0.0009 0.0007 0.0013 0.0015 0.0011 0.0002 0.0009 0.0019 0.0003 0.0004
Bud Neck 0.0007 0.0006 0.001 0.0007 0.0005 0.0018 0.0005 0.0004 0.0005 0.0008 0.0012 0.0018 0.0004 0.0003 0.0007 0.0009 0.0006 0.0014
Bud Periphery 0.0023 0.0005 0.0011 0.0008 0.0003 0.0017 0.0019 0.0006 0.0004 0.0003 0.0008 0.0005 0.0014 0.0001 0.0007 0.0014 0.0001 0.0005
Bud Site 0.0009 0.0005 0.0014 0.0003 0.0004 0.0003 0.0003 0.0005 0.0005 0.0001 0.0008 0.0001 0.0004 0.0002 0.0009 0.0013 0.0004 0
Cell Periphery 0.0225 0.0147 0.0117 0.0067 0.0026 0.0068 0.0122 0.0045 0.0037 0.0021 0.0023 0.0007 0.0041 0.001 0.0033 0.004 0.0005 0.0006
Cytoplasm 0.6184 0.9012 0.7416 0.6524 0.6417 0.7151 0.81 0.9602 0.8877 0.8134 0.6997 0.9038 0.7689 0.9626 0.8297 0.6945 0.7064 0.9056
Cytoplasmic Foci 0.018 0.0011 0.0171 0.0038 0.014 0.0041 0.006 0.0007 0.0067 0.0019 0.0544 0.0042 0.0087 0.0008 0.0101 0.0031 0.0123 0.0033
Eisosomes 0.0005 0.0004 0.0004 0 0.0004 0.0001 0.0003 0 0.0001 0 0.0004 0 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0534 0.0122 0.0419 0.0038 0.0519 0.0498 0.0444 0.0057 0.0101 0.008 0.0048 0.0132 0.0576 0.012 0.0426 0.0048 0.0083 0.0347
Endosome 0.0104 0.0032 0.0113 0.0066 0.0236 0.0306 0.0062 0.0013 0.0079 0.0088 0.0222 0.0208 0.012 0.0015 0.0127 0.0092 0.0553 0.012
Golgi 0.0025 0.0017 0.0055 0.0004 0.0015 0.007 0.0015 0.0002 0.0019 0.0004 0.0045 0.0029 0.0012 0.0002 0.0044 0.0033 0.0051 0.0048
Lipid Particles 0.0087 0.0007 0.0062 0.0014 0.0037 0.0021 0.0024 0.0003 0.002 0.0002 0.0108 0.0002 0.0022 0.0001 0.0017 0.001 0.0043 0
Mitochondria 0.0164 0.0014 0.0099 0.0006 0.0097 0.0049 0.003 0.0003 0.0029 0.0005 0.0426 0.001 0.0098 0.0002 0.0076 0.0101 0.0024 0.0211
None 0.0082 0.0003 0.0053 0.0011 0.0051 0.0008 0.003 0.0001 0.0085 0.0002 0.0386 0.0004 0.0027 0.0001 0.0013 0.0008 0.0061 0.0006
Nuclear Periphery 0.0426 0.0064 0.0112 0.0111 0.0231 0.0349 0.0235 0.0026 0.0062 0.0117 0.0063 0.0047 0.0321 0.0039 0.0078 0.0178 0.0223 0.0041
Nucleolus 0.0086 0.0002 0.0063 0.0013 0.031 0.0023 0.0006 0.0001 0.0012 0.0001 0.0077 0 0.002 0 0.0009 0.0005 0.0003 0
Nucleus 0.0114 0.0022 0.006 0.0061 0.0076 0.0107 0.0063 0.0023 0.0027 0.0049 0.0053 0.0021 0.0084 0.0016 0.0033 0.0064 0.0063 0.0022
Peroxisomes 0.0027 0 0.0018 0.0001 0.001 0.0002 0.0019 0 0.0031 0 0.0034 0.0001 0.0019 0 0.0009 0.0036 0.0014 0
Punctate Nuclear 0.0049 0 0.0011 0.0001 0.0041 0.0009 0.0013 0 0.0003 0 0.0039 0.0001 0.001 0 0.0002 0.0001 0.0125 0
Vacuole 0.1223 0.0447 0.0837 0.2475 0.122 0.0894 0.0593 0.0167 0.0443 0.1133 0.0491 0.031 0.0615 0.0131 0.0489 0.1549 0.1013 0.0049
Vacuole Periphery 0.0375 0.005 0.0288 0.0529 0.0538 0.0297 0.0124 0.0025 0.0073 0.0325 0.0324 0.009 0.0219 0.0015 0.0171 0.0579 0.0476 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 875.2064 1055.9745 1200.3 969.001 1129.5222 799.5924 818.9704 888.4941 910.0478 874.0853
Translational Efficiency 1.8274 1.6683 1.5871 1.4676 1.6635 2.0 1.9395 1.7622 1.6704 1.9482

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl27b

Rpl27b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl27b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available