Standard name
Human Ortholog
Description Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence; JIP4 has a paralog, YOR019W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0.05 0.05 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.99 0.98 0.98 0.96 0.93 – 0.87 0.85 0.7 0.8 0.98 1.0 0.98 0.94 0.93 0.95 0.76 0.85 0.71 0.89 0.81 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.09 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0 0 – 0.14 0.14 0.42 0.26 0 0 0 0 0 0 0.13 0 0.14 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 1 0 1 0 1 1
Bud 0 0 0 0 0 1 2 – 2 3 7 3 0 0 0 0 1 1 0 0 0 0 4 2
Bud Neck 0 0 4 6 8 17 20 – 11 17 8 14 1 2 1 6 5 1 2 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 1 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 6 0 – 1 1 1 1 1 0 0 4 5 4 0 0 0 0 0 1
Cytoplasm 213 26 98 254 264 310 364 – 298 296 194 298 149 314 387 295 271 357 185 57 80 47 112 190
Endoplasmic Reticulum 2 0 0 0 1 4 0 – 0 0 0 1 1 1 2 27 26 35 1 0 0 0 0 0
Endosome 1 0 0 0 0 0 2 – 3 0 0 0 0 0 1 7 6 3 5 1 2 0 1 2
Golgi 0 0 0 2 1 1 0 – 0 0 0 0 0 0 0 0 4 2 0 0 0 0 0 0
Mitochondria 15 1 0 1 0 6 14 – 48 47 116 97 1 0 0 8 4 8 32 2 16 0 4 3
Nucleus 1 0 0 2 1 0 4 – 0 1 0 1 0 0 2 1 1 2 0 0 0 0 1 1
Nuclear Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 1 0 2 7 – 9 13 7 21 1 0 0 2 3 3 2 0 2 0 1 2
Vac/Vac Membrane 0 0 1 1 2 1 2 – 3 0 0 1 0 0 1 4 3 8 5 0 4 0 2 1
Unique Cell Count 226 27 99 258 270 324 390 344 347 277 371 152 315 394 313 292 376 245 67 114 53 138 210
Labelled Cell Count 234 27 104 268 278 349 415 375 380 336 438 154 317 396 354 329 425 245 67 114 53 138 210


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.4 4.8 4.6 5.1 4.0 4.0 – 3.4 3.5 3.0 3.3 4.8 5.1 4.9 5.4 5.3 5.1 4.2 4.9 4.7
Std Deviation (1e-4) 0.6 1.9 1.1 1.1 1.8 1.0 1.2 – 0.9 0.8 0.7 1.0 1.1 1.4 1.5 1.1 1.7 1.3 1.0 1.9 1.4
Intensity Change (Log2) – – – -0.07 0.09 -0.26 -0.27 – -0.5 -0.45 -0.65 -0.53 0.0 0.09 0.04 0.17 0.16 0.08 -0.2 0.05 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1381 -0.491 -0.8222 -0.896 -1.0167 -0.6877 -0.9632 -1.0384 -0.6635 -0.8021 -0.5551 -0.964 -1.5558 -1.7455 -1.9901 -1.3452 -0.3906 -2.1073
Actin 0.0037 0.0002 0.0004 0.0002 0.0002 0.0012 0.0225 0.0013 0.0178 0.0108 0.0026 0.0034 0.0308 0.0002 0.002 0.0506 0.0003 0.0007
Bud 0.0002 0.0034 0.0004 0.0001 0 0 0.0008 0.0046 0.0048 0.0003 0.0002 0.0007 0.0007 0.0029 0.0001 0.0057 0.0001 0.0001
Bud Neck 0.0004 0.003 0.0001 0 0 0.0003 0.012 0.0001 0.0012 0.0002 0.0004 0.0001 0.0026 0 0.0002 0.0089 0.0002 0
Bud Periphery 0.0004 0.0012 0.0003 0.0002 0 0 0.001 0.012 0.0116 0.0009 0.0003 0.0036 0.0008 0.0048 0.0002 0.0055 0.0001 0
Bud Site 0.0003 0.0034 0.0002 0 0 0 0.0093 0.0092 0.0059 0.0013 0.0005 0.0003 0.0212 0.004 0.0008 0.0605 0.0002 0
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0005 0.0004 0.0008 0.0002 0.0001 0 0.0005 0.0001 0.0002 0.0005 0.0001 0
Cytoplasm 0.0119 0.0139 0.0092 0.0109 0.0127 0.025 0.0119 0.0209 0.0153 0.0227 0.01 0.0253 0.0261 0.0274 0.0288 0.0299 0.0547 0.0415
Cytoplasmic Foci 0.0037 0.0079 0.003 0.0036 0.001 0.0041 0.018 0.0035 0.0305 0.0078 0.0044 0.0021 0.0621 0.0015 0.0084 0.0505 0.0326 0.0101
Eisosomes 0.0009 0.0004 0.0004 0.0002 0.0001 0.0001 0.0008 0.0012 0.0004 0.0006 0.0002 0.0001 0.0006 0.0003 0.0005 0.0004 0 0.0001
Endoplasmic Reticulum 0.0012 0.0003 0.0002 0.0003 0.0001 0.0006 0.0082 0.0005 0.0023 0.001 0.0002 0.0001 0.0018 0.0003 0.0008 0.0002 0.001 0.0003
Endosome 0.0023 0.0002 0.0001 0.0002 0 0.001 0.0306 0.0002 0.0149 0.0041 0.0134 0.0001 0.0244 0.0001 0.0052 0.0018 0.2321 0.0003
Golgi 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0053 0.0002 0.0087 0.0037 0.0195 0.0001 0.0132 0 0.0131 0.0045 0.0457 0.0001
Lipid Particles 0.0007 0.0015 0.0003 0.0005 0.0001 0.0002 0.0226 0.0008 0.0148 0.0242 0.0366 0.0002 0.0285 0.0001 0.0084 0.0133 0.0963 0.0002
Mitochondria 0.0027 0.0004 0.0003 0.0003 0.0001 0.0001 0.0043 0.0017 0.0035 0.0469 0.1138 0.0009 0.0164 0.0003 0.0118 0.0018 0.0043 0.0001
None 0.9504 0.9573 0.9815 0.981 0.985 0.9583 0.8132 0.9412 0.8405 0.8281 0.7886 0.9572 0.7271 0.9569 0.9132 0.7169 0.4798 0.9428
Nuclear Periphery 0.006 0.0003 0.0003 0.0003 0.0001 0.0022 0.0114 0.0002 0.0016 0.0018 0.0003 0 0.0016 0.0001 0.0007 0.0001 0.0007 0.0002
Nucleolus 0.0013 0.0007 0.0003 0.0003 0.0001 0.0002 0.0006 0.0003 0.0008 0.0003 0.0002 0 0.0009 0.0002 0.0007 0.0002 0.0004 0
Nucleus 0.0024 0.0022 0.0006 0.0003 0.0001 0.001 0.0078 0.0003 0.0014 0.0003 0.0002 0.0001 0.0012 0.0003 0.001 0.0003 0.0004 0.0002
Peroxisomes 0.0003 0.0002 0.0002 0.0003 0 0.0001 0.013 0.0006 0.0123 0.0428 0.0055 0.0055 0.0313 0.0001 0.0019 0.0468 0.0279 0.0005
Punctate Nuclear 0.0093 0.0029 0.0019 0.0009 0.0003 0.0053 0.0012 0.0003 0.0078 0.001 0.0002 0.0001 0.0034 0.0003 0.0013 0.0014 0.0003 0.0026
Vacuole 0.0006 0.0003 0.0001 0.0002 0 0.0001 0.0034 0.0003 0.0026 0.0006 0.0012 0 0.0037 0.0002 0.0005 0.0002 0.0181 0.0001
Vacuole Periphery 0.0007 0 0 0 0 0.0001 0.0016 0.0001 0.0007 0.0007 0.0017 0 0.0009 0 0.0003 0.0001 0.0048 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.5648 7.1232 5.0075 9.0804 4.8938 8.3831 8.3395 11.7688 11.0845 10.0781
Translational Efficiency 0.6906 0.4603 0.7154 0.4894 0.8178 0.4961 0.5404 0.5471 0.5031 0.496

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence; JIP4 has a paralog, YOR019W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (27%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Jip4

Jip4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Jip4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available