Standard name | |
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Human Ortholog | |
Description | AMP-activated S/T protein kinase; complexes with Snf4p and a Sip1p/Sip2p/Gal83p family member; required for glucose-repressed gene transcription, heat shock, sporulation, and peroxisome biogenesis; active form involved in mitotic spindle alignment in non-limiting glucose; regulates Hxk2p nucleocytoplasmic shuttling; regulates filamentous growth and acts as a non-canonical GEF-activating Arf3p during invasive growth; sumoylation inhibits Snf1p, targeting it for Ub-ligase mediated destruction |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 3 | 3 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 7 | 1 | 1 | 7 | 7 | 7 | – | 0 | 4 | 4 | 6 | 5 | 8 | 12 | 20 | 18 | 23 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 122 | 284 | 16 | 55 | 112 | 120 | 138 | – | 30 | 86 | 85 | 115 | 167 | 268 | 194 | 323 | 245 | 277 | 117 | 257 | 50 | 96 | 249 | 304 |
Endoplasmic Reticulum | 1 | 3 | 0 | 1 | 3 | 5 | 10 | – | 1 | 9 | 2 | 8 | 0 | 4 | 6 | 17 | 10 | 10 | 2 | 4 | 2 | 0 | 1 | 4 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Golgi | 0 | 2 | 0 | 1 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 8 | 5 | – | 4 | 17 | 30 | 24 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 1 | 2 | – | 4 | 0 | 1 | 0 | 1 | 1 | 2 | 4 | 3 | 1 | 0 | 1 | 0 | 2 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 3 | 2 | 2 | – | 1 | 9 | 0 | 8 | 0 | 0 | 0 | 13 | 12 | 13 | 1 | 3 | 0 | 0 | 2 | 2 |
Unique Cell Count | 123 | 291 | 16 | 57 | 118 | 133 | 158 | 38 | 118 | 111 | 148 | 168 | 271 | 199 | 345 | 264 | 294 | 123 | 277 | 57 | 103 | 260 | 319 | |
Labelled Cell Count | 124 | 299 | 17 | 59 | 125 | 144 | 168 | 43 | 129 | 123 | 163 | 173 | 281 | 215 | 380 | 291 | 327 | 123 | 277 | 57 | 103 | 260 | 319 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.1 | 16.1 | 14.2 | 12.5 | 13.8 | 12.0 | 11.9 | – | 8.7 | 9.6 | 9.3 | 9.7 | 13.9 | 14.0 | 14.2 | 21.2 | 20.2 | 19.6 | 12.0 | 12.6 | 13.0 |
Std Deviation (1e-4) | 1.8 | 2.8 | 1.7 | 2.7 | 1.5 | 2.1 | 1.8 | – | 1.8 | 1.9 | 1.7 | 1.7 | 1.8 | 1.7 | 2.0 | 4.3 | 4.3 | 4.6 | 1.6 | 1.7 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.04 | -0.24 | -0.25 | – | -0.71 | -0.56 | -0.61 | -0.54 | -0.03 | -0.01 | 0.0 | 0.58 | 0.51 | 0.47 | -0.24 | -0.17 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.8 | -0.9 | -1.3 | -1.5 | – | -2.0 | -2.4 | -2.2 | -2.1 | -0.3 | -0.4 | -0.6 | -1.0 | -1.1 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.5953 | 8.795 | 8.1867 | 6.4081 | 7.1329 | 8.2279 | 8.5789 | 11.4003 | 9.6479 | 9.1946 | 7.2462 | 9.8638 | 6.5244 | 10.5435 | 8.8041 | 8.3578 | 8.1884 | 9.0992 |
Actin | 0.0141 | 0.0058 | 0.0009 | 0.0121 | 0.0057 | 0.0008 | 0.0259 | 0.0001 | 0.0033 | 0.0001 | 0.0966 | 0.0008 | 0.0486 | 0.0007 | 0.0176 | 0.0007 | 0.0405 | 0.0028 |
Bud | 0.0009 | 0.0011 | 0.0003 | 0.0006 | 0.0038 | 0.0005 | 0.001 | 0.0001 | 0.0021 | 0.0003 | 0.0025 | 0.0003 | 0.0017 | 0.0084 | 0.0007 | 0.0002 | 0.0007 | 0.0002 |
Bud Neck | 0.0009 | 0.0002 | 0.0002 | 0.003 | 0.001 | 0.0008 | 0.0011 | 0.0001 | 0.0009 | 0.0003 | 0.0014 | 0.0007 | 0.0036 | 0.0001 | 0.0005 | 0.0006 | 0.0008 | 0.0005 |
Bud Periphery | 0.0009 | 0.0006 | 0.0001 | 0.0006 | 0.0058 | 0.0004 | 0.0011 | 0 | 0.0012 | 0.0001 | 0.0052 | 0.0003 | 0.0019 | 0.0021 | 0.0007 | 0.0001 | 0.0007 | 0.0001 |
Bud Site | 0.0066 | 0.003 | 0.0004 | 0.0018 | 0.0131 | 0.0001 | 0.0027 | 0.0002 | 0.001 | 0.0002 | 0.0083 | 0.0001 | 0.0033 | 0.0019 | 0.0009 | 0.0004 | 0.0007 | 0.0001 |
Cell Periphery | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.8534 | 0.9485 | 0.942 | 0.7749 | 0.7042 | 0.9222 | 0.8144 | 0.9764 | 0.8569 | 0.8973 | 0.6517 | 0.9071 | 0.6974 | 0.9626 | 0.8268 | 0.859 | 0.7769 | 0.9133 |
Cytoplasmic Foci | 0.0165 | 0.0066 | 0.006 | 0.0218 | 0.0483 | 0.0082 | 0.0136 | 0.0034 | 0.0142 | 0.0097 | 0.0299 | 0.0079 | 0.0802 | 0.0059 | 0.0246 | 0.0099 | 0.0361 | 0.0087 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0009 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0147 | 0.0065 | 0.0159 | 0.0106 | 0.0132 | 0.0067 | 0.0548 | 0.0073 | 0.0339 | 0.0061 | 0.0145 | 0.038 | 0.0252 | 0.0044 | 0.0393 | 0.0238 | 0.0368 | 0.01 |
Endosome | 0.041 | 0.0086 | 0.0177 | 0.0727 | 0.0767 | 0.0197 | 0.034 | 0.0077 | 0.0473 | 0.0521 | 0.0928 | 0.0239 | 0.0387 | 0.0076 | 0.0308 | 0.0496 | 0.0495 | 0.0391 |
Golgi | 0.0137 | 0.0029 | 0.0009 | 0.007 | 0.0158 | 0.0025 | 0.0067 | 0.0003 | 0.0033 | 0.0009 | 0.0217 | 0.003 | 0.0116 | 0.0016 | 0.0103 | 0.0006 | 0.0218 | 0.0047 |
Lipid Particles | 0.0044 | 0.0035 | 0.0002 | 0.0056 | 0.0074 | 0 | 0.0035 | 0 | 0.0003 | 0.0001 | 0.0079 | 0.0008 | 0.018 | 0.0001 | 0.003 | 0.0002 | 0.0049 | 0.0032 |
Mitochondria | 0.0042 | 0.0067 | 0.0002 | 0.0056 | 0.0176 | 0.0321 | 0.0038 | 0.0001 | 0.0032 | 0.0002 | 0.0189 | 0.0052 | 0.0055 | 0.0001 | 0.0058 | 0.0002 | 0.0041 | 0.0013 |
None | 0.0014 | 0.0007 | 0.0007 | 0.001 | 0.0008 | 0.0004 | 0.0057 | 0.0005 | 0.0011 | 0.0011 | 0.0019 | 0.0014 | 0.0154 | 0.0007 | 0.0047 | 0.0124 | 0.0011 | 0.0009 |
Nuclear Periphery | 0.0031 | 0.0005 | 0.0035 | 0.0049 | 0.0027 | 0.0005 | 0.0067 | 0.0005 | 0.0056 | 0.0017 | 0.0051 | 0.0024 | 0.0064 | 0.0005 | 0.0083 | 0.0034 | 0.0144 | 0.0063 |
Nucleolus | 0.0002 | 0 | 0 | 0.0003 | 0.0006 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.0017 | 0.0006 | 0.0009 | 0.0023 | 0.0018 | 0.0007 | 0.0044 | 0.0005 | 0.0021 | 0.0013 | 0.0034 | 0.001 | 0.0021 | 0.0006 | 0.0022 | 0.0022 | 0.0016 | 0.0012 |
Peroxisomes | 0.0025 | 0.0008 | 0 | 0.0425 | 0.017 | 0.0001 | 0.0016 | 0 | 0.0002 | 0 | 0.003 | 0.0002 | 0.0182 | 0.0001 | 0.0024 | 0.0001 | 0.0024 | 0.0005 |
Punctate Nuclear | 0.0006 | 0.0002 | 0.0001 | 0.0005 | 0.0014 | 0 | 0.0017 | 0 | 0.0003 | 0.0001 | 0.0014 | 0.0003 | 0.0133 | 0.0001 | 0.0085 | 0.0008 | 0.002 | 0.0043 |
Vacuole | 0.0158 | 0.0024 | 0.0083 | 0.0235 | 0.0517 | 0.0027 | 0.0141 | 0.0025 | 0.0185 | 0.0233 | 0.023 | 0.0044 | 0.0055 | 0.0022 | 0.0103 | 0.0328 | 0.0027 | 0.0018 |
Vacuole Periphery | 0.0029 | 0.0005 | 0.0015 | 0.0081 | 0.0107 | 0.0014 | 0.0022 | 0.0004 | 0.0044 | 0.0051 | 0.0084 | 0.0019 | 0.0015 | 0.0003 | 0.0021 | 0.0031 | 0.0018 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.3574 | 30.5974 | 24.2439 | 31.1674 | 30.0865 | 23.1923 | 31.4613 | 41.5838 | 32.8829 | 37.1652 |
Translational Efficiency | 0.9859 | 1.0196 | 0.939 | 0.7728 | 0.6654 | 1.7656 | 1.4008 | 0.881 | 1.1136 | 0.936 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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