Standard name
Human Ortholog
Description AMP-activated S/T protein kinase; complexes with Snf4p and a Sip1p/Sip2p/Gal83p family member; required for glucose-repressed gene transcription, heat shock, sporulation, and peroxisome biogenesis; active form involved in mitotic spindle alignment in non-limiting glucose; regulates Hxk2p nucleocytoplasmic shuttling; regulates filamentous growth and acts as a non-canonical GEF-activating Arf3p during invasive growth; sumoylation inhibits Snf1p, targeting it for Ub-ligase mediated destruction

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0.06 0.06 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.96 0.95 0.9 0.87 0.79 0.73 0.77 0.78 0.99 0.99 0.97 0.94 0.93 0.94 0.96 0.93 0.88 0.94 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 0.08 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0 0.11 0.14 0.27 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 3 3 1 2 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 7 1 1 7 7 7 0 4 4 6 5 8 12 20 18 23 0 0 0 0 0 0
Cytoplasm 122 284 16 55 112 120 138 30 86 85 115 167 268 194 323 245 277 117 257 50 96 249 304
Endoplasmic Reticulum 1 3 0 1 3 5 10 1 9 2 8 0 4 6 17 10 10 2 4 2 0 1 4
Endosome 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 2 0 0 0 1
Golgi 0 2 0 1 0 1 1 0 0 0 0 0 0 0 1 1 2 0 1 0 0 0 0
Mitochondria 1 0 0 0 0 8 5 4 17 30 24 0 0 0 2 1 0 0 2 0 0 0 0
Nucleus 0 1 0 1 0 1 2 4 0 1 0 1 1 2 4 3 1 0 1 0 2 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 3 2 2 1 9 0 8 0 0 0 13 12 13 1 3 0 0 2 2
Unique Cell Count 123 291 16 57 118 133 158 38 118 111 148 168 271 199 345 264 294 123 277 57 103 260 319
Labelled Cell Count 124 299 17 59 125 144 168 43 129 123 163 173 281 215 380 291 327 123 277 57 103 260 319


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.1 16.1 14.2 12.5 13.8 12.0 11.9 8.7 9.6 9.3 9.7 13.9 14.0 14.2 21.2 20.2 19.6 12.0 12.6 13.0
Std Deviation (1e-4) 1.8 2.8 1.7 2.7 1.5 2.1 1.8 1.8 1.9 1.7 1.7 1.8 1.7 2.0 4.3 4.3 4.6 1.6 1.7 2.0
Intensity Change (Log2) -0.18 -0.04 -0.24 -0.25 -0.71 -0.56 -0.61 -0.54 -0.03 -0.01 0.0 0.58 0.51 0.47 -0.24 -0.17 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -0.9 -1.3 -1.5 -2.0 -2.4 -2.2 -2.1 -0.3 -0.4 -0.6 -1.0 -1.1 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5953 8.795 8.1867 6.4081 7.1329 8.2279 8.5789 11.4003 9.6479 9.1946 7.2462 9.8638 6.5244 10.5435 8.8041 8.3578 8.1884 9.0992
Actin 0.0141 0.0058 0.0009 0.0121 0.0057 0.0008 0.0259 0.0001 0.0033 0.0001 0.0966 0.0008 0.0486 0.0007 0.0176 0.0007 0.0405 0.0028
Bud 0.0009 0.0011 0.0003 0.0006 0.0038 0.0005 0.001 0.0001 0.0021 0.0003 0.0025 0.0003 0.0017 0.0084 0.0007 0.0002 0.0007 0.0002
Bud Neck 0.0009 0.0002 0.0002 0.003 0.001 0.0008 0.0011 0.0001 0.0009 0.0003 0.0014 0.0007 0.0036 0.0001 0.0005 0.0006 0.0008 0.0005
Bud Periphery 0.0009 0.0006 0.0001 0.0006 0.0058 0.0004 0.0011 0 0.0012 0.0001 0.0052 0.0003 0.0019 0.0021 0.0007 0.0001 0.0007 0.0001
Bud Site 0.0066 0.003 0.0004 0.0018 0.0131 0.0001 0.0027 0.0002 0.001 0.0002 0.0083 0.0001 0.0033 0.0019 0.0009 0.0004 0.0007 0.0001
Cell Periphery 0.0003 0.0003 0.0002 0.0002 0.0006 0.0001 0.0007 0.0001 0.0003 0.0001 0.0007 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0
Cytoplasm 0.8534 0.9485 0.942 0.7749 0.7042 0.9222 0.8144 0.9764 0.8569 0.8973 0.6517 0.9071 0.6974 0.9626 0.8268 0.859 0.7769 0.9133
Cytoplasmic Foci 0.0165 0.0066 0.006 0.0218 0.0483 0.0082 0.0136 0.0034 0.0142 0.0097 0.0299 0.0079 0.0802 0.0059 0.0246 0.0099 0.0361 0.0087
Eisosomes 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0 0 0.0009 0 0.0006 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0147 0.0065 0.0159 0.0106 0.0132 0.0067 0.0548 0.0073 0.0339 0.0061 0.0145 0.038 0.0252 0.0044 0.0393 0.0238 0.0368 0.01
Endosome 0.041 0.0086 0.0177 0.0727 0.0767 0.0197 0.034 0.0077 0.0473 0.0521 0.0928 0.0239 0.0387 0.0076 0.0308 0.0496 0.0495 0.0391
Golgi 0.0137 0.0029 0.0009 0.007 0.0158 0.0025 0.0067 0.0003 0.0033 0.0009 0.0217 0.003 0.0116 0.0016 0.0103 0.0006 0.0218 0.0047
Lipid Particles 0.0044 0.0035 0.0002 0.0056 0.0074 0 0.0035 0 0.0003 0.0001 0.0079 0.0008 0.018 0.0001 0.003 0.0002 0.0049 0.0032
Mitochondria 0.0042 0.0067 0.0002 0.0056 0.0176 0.0321 0.0038 0.0001 0.0032 0.0002 0.0189 0.0052 0.0055 0.0001 0.0058 0.0002 0.0041 0.0013
None 0.0014 0.0007 0.0007 0.001 0.0008 0.0004 0.0057 0.0005 0.0011 0.0011 0.0019 0.0014 0.0154 0.0007 0.0047 0.0124 0.0011 0.0009
Nuclear Periphery 0.0031 0.0005 0.0035 0.0049 0.0027 0.0005 0.0067 0.0005 0.0056 0.0017 0.0051 0.0024 0.0064 0.0005 0.0083 0.0034 0.0144 0.0063
Nucleolus 0.0002 0 0 0.0003 0.0006 0 0.0003 0 0.0001 0 0.0008 0.0001 0.0007 0 0.0001 0.0001 0.0002 0.0001
Nucleus 0.0017 0.0006 0.0009 0.0023 0.0018 0.0007 0.0044 0.0005 0.0021 0.0013 0.0034 0.001 0.0021 0.0006 0.0022 0.0022 0.0016 0.0012
Peroxisomes 0.0025 0.0008 0 0.0425 0.017 0.0001 0.0016 0 0.0002 0 0.003 0.0002 0.0182 0.0001 0.0024 0.0001 0.0024 0.0005
Punctate Nuclear 0.0006 0.0002 0.0001 0.0005 0.0014 0 0.0017 0 0.0003 0.0001 0.0014 0.0003 0.0133 0.0001 0.0085 0.0008 0.002 0.0043
Vacuole 0.0158 0.0024 0.0083 0.0235 0.0517 0.0027 0.0141 0.0025 0.0185 0.0233 0.023 0.0044 0.0055 0.0022 0.0103 0.0328 0.0027 0.0018
Vacuole Periphery 0.0029 0.0005 0.0015 0.0081 0.0107 0.0014 0.0022 0.0004 0.0044 0.0051 0.0084 0.0019 0.0015 0.0003 0.0021 0.0031 0.0018 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.3574 30.5974 24.2439 31.1674 30.0865 23.1923 31.4613 41.5838 32.8829 37.1652
Translational Efficiency 0.9859 1.0196 0.939 0.7728 0.6654 1.7656 1.4008 0.881 1.1136 0.936

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description AMP-activated S/T protein kinase; complexes with Snf4p and a Sip1p/Sip2p/Gal83p family member; required for glucose-repressed gene transcription, heat shock, sporulation, and peroxisome biogenesis; active form involved in mitotic spindle alignment in non-limiting glucose; regulates Hxk2p nucleocytoplasmic shuttling; regulates filamentous growth and acts as a non-canonical GEF-activating Arf3p during invasive growth; sumoylation inhibits Snf1p, targeting it for Ub-ligase mediated destruction
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Snf1

Snf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available