Standard name | |
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Human Ortholog | |
Description | Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 4 | 2 | 3 | 2 | 4 | 9 | 2 | 15 | 13 | 25 | 27 | 20 | – | – | – | 0 | 0 | 3 | 1 | 2 | 1 |
Bud | 0 | 1 | 3 | 6 | 2 | 6 | 5 | 6 | 15 | 11 | 25 | 27 | 3 | 7 | 5 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 3 | 3 | 1 | 5 | 4 | 2 | 2 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 21 | 28 | 21 | 53 | 73 | 101 | 112 | 106 | 69 | 70 | 79 | 12 | 53 | 63 | – | – | – | 0 | 0 | 0 | 0 | 1 | 5 |
Endoplasmic Reticulum | 3 | 3 | 8 | 2 | 5 | 9 | 12 | 21 | 5 | 11 | 11 | 11 | 46 | 35 | 12 | – | – | – | 0 | 0 | 0 | 1 | 5 | 7 |
Endosome | 1 | 0 | 92 | 113 | 96 | 72 | 72 | 127 | 90 | 55 | 61 | 121 | 59 | 50 | 63 | – | – | – | 11 | 17 | 146 | 81 | 108 | 78 |
Golgi | 0 | 0 | 35 | 61 | 47 | 37 | 38 | 26 | 20 | 8 | 13 | 36 | 32 | 16 | 10 | – | – | – | 6 | 5 | 142 | 95 | 123 | 89 |
Mitochondria | 8 | 38 | 124 | 87 | 53 | 107 | 127 | 114 | 172 | 178 | 185 | 176 | 34 | 8 | 10 | – | – | – | 4 | 5 | 17 | 23 | 43 | 25 |
Nucleus | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 5 | 4 | 2 | 1 | 1 | 0 | 2 | 2 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 0 | 3 | 0 | 2 | 1 | 5 | 6 | 1 | 2 | 2 | 4 | 3 | 1 | 0 | – | – | – | 1 | 0 | 3 | 1 | 3 | 1 |
Nucleolus | 0 | 0 | 4 | 5 | 13 | 26 | 32 | 39 | 26 | 30 | 21 | 25 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 5 | 1 | 3 | 7 | 4 | 11 | 5 | 3 | 8 | 6 | 0 | 0 | 2 | – | – | – | 0 | 0 | 2 | 5 | 2 | 0 |
SpindlePole | 0 | 0 | 6 | 11 | 4 | 5 | 12 | 11 | 13 | 7 | 10 | 23 | 12 | 7 | 16 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 49 | 62 | 283 | 108 | 251 | 186 | 270 | 300 | 154 | 160 | 115 | 119 | 75 | 77 | 61 | – | – | – | 69 | 88 | 167 | 79 | 275 | 343 |
Unique Cell Count | 58 | 101 | 450 | 308 | 413 | 408 | 530 | 643 | 480 | 438 | 440 | 509 | 234 | 226 | 225 | 96 | 121 | 487 | 291 | 575 | 561 | |||
Labelled Cell Count | 66 | 126 | 595 | 419 | 532 | 533 | 681 | 785 | 623 | 540 | 544 | 647 | 303 | 285 | 266 | 96 | 121 | 487 | 291 | 575 | 561 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 6.4 | 7.7 | 9.7 | 10.7 | 9.9 | 9.4 | 9.4 | 9.1 | 8.3 | 8.8 | 8.8 | 7.8 | 7.3 | 6.9 | – | – | – | 9.7 | 9.0 | 9.6 |
Std Deviation (1e-4) | 1.0 | 1.3 | 1.4 | 1.7 | 2.2 | 2.1 | 2.1 | 2.2 | 2.2 | 2.0 | 1.9 | 1.7 | 0.9 | 0.8 | 0.9 | – | – | – | 1.8 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.34 | 0.48 | 0.36 | 0.3 | 0.3 | 0.25 | 0.11 | 0.2 | 0.2 | 0.03 | -0.07 | -0.15 | – | – | – | 0.34 | 0.24 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 3.0 | 6.8 | 7.2 | 6.1 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 2.7 | 2.2 | 4.3 | 4.1 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 0.3 | 3.3 | 5.3 | 5.9 | 5.5 | 6.9 | 4.6 | 4.6 | 4.6 | -0.6 | 6.5 | 7.8 | – | – | – |
Endoplasmic Reticulum | 0 | -0.7 | 0.4 | 0.5 | 1.5 | -1.0 | 0.8 | 0.7 | 0.4 | 8.2 | 6.9 | 2.6 | – | – | – |
Endosome | 4.9 | 1.0 | -1.0 | -2.9 | -0.3 | -0.7 | -3.2 | -2.6 | 1.2 | 1.4 | 0.5 | 2.2 | – | – | – |
Golgi | 4.9 | 1.8 | 0.7 | -0.4 | -2.6 | -2.3 | -4.1 | -3.2 | -0.4 | 2.5 | -0.3 | -1.6 | – | – | – |
Mitochondria | 0.2 | -5.4 | -0.4 | -1.3 | -3.9 | 2.7 | 4.1 | 4.5 | 2.3 | -3.8 | -7.4 | -7.1 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 2.4 | 4.4 | 4.3 | 4.3 | 3.9 | 4.6 | 3.5 | 3.6 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0.8 | 0 | 0.8 | 0 | 0 | 0.9 | 0.1 | 0 | 0 | 0 | – | – | – |
SpindlePole | 2.0 | 0 | -0.1 | 1.1 | 0.5 | 1.5 | 0.3 | 1.1 | 2.9 | 2.9 | 0 | 4.0 | – | – | – |
Vacuole | -7.5 | -0.6 | -5.1 | -3.8 | -5.3 | -9.4 | -7.9 | -11.0 | -12.4 | -7.7 | -7.1 | -8.8 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.3461 | 9.2326 | 8.7059 | 6.9498 | 7.3772 | 8.4984 | 8.2038 | 10.4318 | 9.8245 | 8.6096 | 8.4531 | 9.969 | 9.4995 | 11.6953 | 10.16 | 9.4912 | 9.5081 | 10.46 |
Actin | 0.0122 | 0.0013 | 0.0011 | 0.009 | 0.0003 | 0.0007 | 0.0206 | 0.0007 | 0.0076 | 0.0004 | 0.0122 | 0.0039 | 0.0388 | 0.0003 | 0.0078 | 0.0608 | 0.0004 | 0.0026 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0002 |
Bud Neck | 0.0003 | 0.0003 | 0.0002 | 0.0108 | 0.0001 | 0.0003 | 0.001 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0005 |
Bud Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0015 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0017 | 0.0004 |
Bud Site | 0.0002 | 0.0002 | 0.0001 | 0.0019 | 0 | 0.0002 | 0.0029 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0016 | 0.0002 | 0.0003 | 0.0005 | 0.0017 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0013 | 0.0003 |
Cytoplasm | 0.0013 | 0.0045 | 0.0021 | 0.0011 | 0.0006 | 0.0011 | 0.0021 | 0.002 | 0.0019 | 0.0038 | 0.0013 | 0.0009 | 0.0021 | 0.0012 | 0.001 | 0.0014 | 0.001 | 0.0008 |
Cytoplasmic Foci | 0.0398 | 0.033 | 0.0292 | 0.0281 | 0.0306 | 0.0217 | 0.0453 | 0.0231 | 0.0256 | 0.0204 | 0.0301 | 0.0183 | 0.0229 | 0.0178 | 0.0141 | 0.0207 | 0.0207 | 0.0119 |
Eisosomes | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0016 | 0.0022 | 0.0107 | 0.0007 | 0.0008 | 0.002 | 0.004 | 0.0035 | 0.0032 | 0.0031 | 0.0023 | 0.0029 | 0.0041 | 0.0029 | 0.0023 | 0.003 | 0.0013 | 0.0027 |
Endosome | 0.3669 | 0.4576 | 0.4234 | 0.4752 | 0.4044 | 0.3947 | 0.3238 | 0.4305 | 0.3386 | 0.461 | 0.5178 | 0.334 | 0.3549 | 0.3605 | 0.3574 | 0.437 | 0.5384 | 0.3341 |
Golgi | 0.4583 | 0.4108 | 0.4426 | 0.3165 | 0.4684 | 0.3715 | 0.4501 | 0.3752 | 0.4628 | 0.3077 | 0.2975 | 0.4671 | 0.3676 | 0.4001 | 0.4063 | 0.2346 | 0.1969 | 0.4375 |
Lipid Particles | 0.037 | 0.0046 | 0.0087 | 0.0064 | 0.0052 | 0.0138 | 0.027 | 0.0051 | 0.0061 | 0.0052 | 0.0253 | 0.0144 | 0.0256 | 0.0067 | 0.0066 | 0.0129 | 0.009 | 0.0249 |
Mitochondria | 0.0266 | 0.0086 | 0.0124 | 0.0347 | 0.0026 | 0.0158 | 0.0572 | 0.0286 | 0.0575 | 0.0355 | 0.0218 | 0.0388 | 0.0965 | 0.0378 | 0.0246 | 0.0166 | 0.0113 | 0.0277 |
None | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0113 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.005 | 0.0012 | 0.001 | 0.0011 | 0.0011 | 0.0029 | 0.0019 | 0.0037 | 0.0036 | 0.0023 | 0.0023 | 0.0021 | 0.0014 | 0.0036 | 0.0034 | 0.0019 | 0.0015 | 0.002 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0017 | 0.0001 |
Nucleus | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0012 | 0.0003 |
Peroxisomes | 0.0054 | 0.0007 | 0.0005 | 0.0061 | 0.0004 | 0.0006 | 0.0144 | 0.0002 | 0.0016 | 0.0012 | 0.0034 | 0.0021 | 0.0054 | 0.0004 | 0.0009 | 0.0078 | 0.0013 | 0.0004 |
Punctate Nuclear | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0027 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0007 | 0.0002 | 0.0001 |
Vacuole | 0.0212 | 0.0412 | 0.0329 | 0.0509 | 0.0341 | 0.0817 | 0.0192 | 0.0501 | 0.0408 | 0.0484 | 0.0267 | 0.0426 | 0.0295 | 0.0672 | 0.0683 | 0.0692 | 0.115 | 0.0578 |
Vacuole Periphery | 0.0232 | 0.033 | 0.0346 | 0.0546 | 0.0508 | 0.0917 | 0.0277 | 0.0765 | 0.0486 | 0.0984 | 0.0542 | 0.0693 | 0.046 | 0.0996 | 0.1051 | 0.1302 | 0.0939 | 0.0954 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 249.9057 | 201.8768 | 205.9768 | 204.6771 | 207.7563 | 241.4695 | 214.6235 | 184.3627 | 203.4436 | 211.2997 |
Translational Efficiency | 0.9139 | 0.8773 | 0.832 | 0.8172 | 0.7739 | 0.8609 | 0.7304 | 0.8863 | 0.8608 | 0.7876 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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