Standard name
Human Ortholog
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0.06 0.08 – 0.11 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.06 0 0 0 – 0 0 0 0 0 0 0.14 0.17 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.26 0.13 0.25 0.15 0.17 0 0 0 – 0 0 0 0.33 0.3 0.39 0.37 0.32 0.37 0.31 0.28 0.27 0.21 0.18 0.15
Golgi 0.14 0.12 0 0 0 0 0 0 – 0 0 0 0.36 0.39 0.28 0.08 0.14 0.21 0.32 0.39 0.28 0.27 0.31 0.38
Mitochondria 0.66 0.8 0.56 0.75 0.69 0.93 0.92 0.89 – 0.92 0.94 0.92 0.2 0.15 0.08 0.1 0.09 0.05 0.05 0.08 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0.06 0 0.08 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Peroxisomes 0.05 0.09 0.23 0.16 0.1 0.05 0.08 0.11 – 0.06 0.07 0.05 0.11 0.12 0.12 0.3 0.22 0.17 0.25 0.16 0.33 0.41 0.44 0.36
SpindlePole 0.12 0.05 0.22 0.09 0.12 0 0.08 0.11 – 0.08 0.06 0.07 0.16 0.17 0.21 0.28 0.21 0.17 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.1 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 25 5 2 1 1 0 5 – 0 0 0 27 19 17 2 5 0 8 11 9 5 6 11
Bud 5 16 11 5 6 14 19 32 – 47 23 17 8 9 11 7 8 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 1 1 2 2 0 0 0 0 0 0 0 0
Bud Site 0 2 0 1 0 3 5 13 – 11 8 10 0 1 0 0 1 0 – – – – – –
Cell Periphery 4 1 0 1 1 0 1 1 – 0 1 2 2 3 5 2 1 0 0 0 0 0 0 0
Cytoplasm 6 9 10 13 10 9 8 16 – 7 5 8 11 14 25 39 49 23 0 1 0 0 0 2
Endoplasmic Reticulum 5 8 2 0 1 0 0 1 – 0 0 0 5 8 2 2 7 6 0 0 0 1 1 1
Endosome 100 56 80 28 28 2 5 3 – 1 3 2 192 261 337 99 90 43 137 131 96 77 67 62
Golgi 53 52 13 3 3 1 0 0 – 0 1 0 207 336 244 22 40 24 142 182 102 100 117 161
Mitochondria 252 341 178 139 112 268 307 364 – 387 218 294 117 133 73 26 26 6 22 38 17 16 10 18
Nucleus 0 0 1 2 0 3 4 1 – 5 0 1 0 4 4 1 2 1 1 0 1 0 0 1
Nuclear Periphery 0 0 2 0 2 4 4 2 – 5 2 4 0 1 2 1 4 1 1 0 0 0 0 0
Nucleolus 11 6 5 1 3 7 14 8 – 25 7 25 4 11 18 16 13 0 0 0 0 0 0 1
Peroxisomes 21 37 72 29 16 15 26 44 – 27 16 16 64 102 103 81 64 20 111 76 118 150 168 151
SpindlePole 46 22 70 16 20 11 27 44 – 33 13 22 95 147 181 77 59 20 15 4 10 10 2 0
Vac/Vac Membrane 12 12 1 0 2 3 3 6 – 1 0 3 25 40 83 7 8 3 3 10 1 1 1 3
Unique Cell Count 383 426 318 186 162 287 334 408 420 233 320 577 859 866 271 285 116 446 465 362 367 380 421
Labelled Cell Count 521 587 450 240 205 341 423 540 549 297 404 758 1090 1107 384 377 148 446 465 362 367 380 421


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 6.5 6.0 5.4 5.2 4.2 4.1 4.2 – 3.7 3.9 3.9 7.7 7.8 8.0 6.4 7.3 8.0 7.6 7.8 8.3
Std Deviation (1e-4) 0.8 0.8 1.1 1.4 1.8 1.0 1.2 1.0 – 0.9 0.8 0.9 0.8 0.8 0.9 1.2 1.3 1.5 1.4 1.1 1.5
Intensity Change (Log2) – – – -0.14 -0.19 -0.5 -0.54 -0.49 – -0.68 -0.6 -0.62 0.37 0.39 0.43 0.11 0.29 0.43 0.36 0.38 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 2.4 0.7 0.4 0 0 0
Bud -0.5 0.1 0 0 0 – 0 0 0 -2.1 -2.8 -2.5 -0.6 -0.5 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 1.6 0 -0.6 0.6 – -1.3 -0.7 -0.5 -1.2 -1.6 -0.2 4.9 5.8 5.8
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome -2.7 -2.0 0 0 0 – 0 0 0 2.5 1.8 4.4 3.0 1.7 2.4
Golgi -1.5 -1.3 0 0 0 – 0 0 0 10.6 11.7 8.9 2.1 4.3 5.5
Mitochondria 4.2 2.8 0 0 0 – 0 0 0 -10.9 -14.0 -17.7 -11.8 -12.1 -9.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0.6 2.8 2.2 0
Peroxisomes -1.9 -3.4 0 0 0 – 0 0 0 -4.6 -4.6 -4.6 2.0 -0.1 -1.2
SpindlePole -3.9 -2.6 0 0 0 – 0 0 0 -2.0 -1.9 -0.4 1.8 -0.4 -1.1
Vacuole 0 0 0 0 0 – 0 0 0 3.4 3.6 5.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8629 1.5767 1.2069 1.3996 1.7169 1.4453 1.3634 1.9582 1.5264 1.313 1.0629 1.7268 0.8527 1.3005 1.2073 0.9018 0.9344 1.1359
Actin 0.0041 0.0134 0.0039 0.001 0.0131 0.0074 0.0119 0.0021 0.0122 0.0007 0.0004 0.0037 0.0307 0.0257 0.0155 0.0019 0.0081 0.042
Bud 0.0002 0.0011 0.0028 0.0001 0.0008 0.0001 0.0026 0.0002 0.0008 0.0001 0 0.0017 0.0003 0.0013 0.0004 0.0001 0.0001 0.0009
Bud Neck 0.0008 0.0009 0.0005 0.0003 0.0015 0.0008 0.0006 0.0002 0.0003 0.0002 0.0001 0.0003 0.0013 0.0005 0.0005 0.0004 0.0004 0.0011
Bud Periphery 0.0002 0.0009 0.0009 0.0001 0.0019 0.0001 0.0006 0.0001 0.0004 0.0001 0 0.0005 0.0004 0.0012 0.0003 0 0.0001 0.0007
Bud Site 0.0014 0.0156 0.0019 0.0003 0.0005 0.0003 0.0033 0.0007 0.0009 0.0004 0.0001 0.0003 0.0028 0.0041 0.0012 0.0005 0.0004 0.0011
Cell Periphery 0.0001 0.0002 0.0001 0 0.0002 0 0.0002 0 0.0001 0 0 0 0.0002 0.0001 0 0 0 0.0001
Cytoplasm 0.0045 0.0083 0.0046 0.0011 0.0021 0.0007 0.0022 0.002 0.0015 0.0021 0.0005 0.0009 0.0037 0.0029 0.0016 0.0006 0.0006 0.0011
Cytoplasmic Foci 0.1689 0.2416 0.1596 0.1433 0.1135 0.1322 0.1463 0.1896 0.1495 0.1319 0.1416 0.1261 0.1558 0.2182 0.1871 0.1646 0.2103 0.1571
Eisosomes 0.0001 0.0001 0 0 0.0001 0 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0.0002
Endoplasmic Reticulum 0.0006 0.0004 0.0003 0.0002 0.0014 0.0003 0.0015 0.0001 0.0001 0.0001 0.0001 0.0002 0.0034 0.0003 0.0002 0.0001 0.0001 0.0013
Endosome 0.2289 0.2275 0.2034 0.2393 0.1861 0.1105 0.1392 0.1624 0.1321 0.1149 0.1572 0.1048 0.1612 0.2039 0.1888 0.1708 0.2008 0.1011
Golgi 0.1468 0.1796 0.1967 0.2064 0.2191 0.154 0.1492 0.1822 0.1795 0.192 0.1676 0.1813 0.1627 0.238 0.294 0.2752 0.2133 0.2423
Lipid Particles 0.1408 0.0612 0.0975 0.0875 0.1493 0.1626 0.0901 0.0712 0.0588 0.1225 0.1049 0.0919 0.1965 0.0608 0.0503 0.1027 0.0881 0.1346
Mitochondria 0.0319 0.0309 0.0492 0.0679 0.0254 0.0168 0.0204 0.0241 0.0308 0.0375 0.0156 0.0318 0.0267 0.0155 0.0276 0.0075 0.003 0.0291
None 0.0029 0.0054 0.0013 0.0001 0.0012 0.0002 0.0032 0.0081 0.0011 0.0285 0.0001 0.0006 0.001 0.0003 0.0002 0.0001 0.0001 0.0008
Nuclear Periphery 0.0008 0.0003 0.0002 0.0001 0.0188 0.0003 0.0033 0.0001 0.0002 0.0002 0 0.0005 0.0121 0.0001 0.0001 0 0 0.0041
Nucleolus 0.0003 0.0021 0.0006 0.0001 0.0022 0.0001 0.0007 0.0001 0.0003 0.0002 0 0.0002 0.0005 0.0002 0.0001 0.0018 0 0.0005
Nucleus 0.0003 0.0006 0.0004 0 0.0219 0.0001 0.0008 0.0001 0.0002 0.0001 0 0.0001 0.0034 0.0001 0.0001 0.0001 0 0.0006
Peroxisomes 0.2493 0.2025 0.2721 0.2499 0.2327 0.4107 0.4191 0.3549 0.4277 0.3664 0.4102 0.4518 0.2322 0.2234 0.2297 0.2709 0.2736 0.2768
Punctate Nuclear 0.0032 0.0034 0.0013 0.0004 0.002 0.0011 0.0027 0.0006 0.0025 0.0005 0.0003 0.0022 0.0018 0.0012 0.0005 0.0016 0.0003 0.0025
Vacuole 0.0126 0.0031 0.0017 0.0011 0.0034 0.0007 0.0011 0.0007 0.0006 0.0008 0.0005 0.0006 0.0016 0.0014 0.0011 0.0007 0.0004 0.001
Vacuole Periphery 0.0014 0.001 0.001 0.0009 0.0029 0.0007 0.0008 0.0005 0.0005 0.0006 0.0005 0.0006 0.0015 0.0007 0.0008 0.0006 0.0004 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.5883 3.0728 5.3204 11.0403 4.4567 5.7258 9.3208 12.9313 9.3953 11.0563
Translational Efficiency 0.9623 1.9516 1.3892 0.4872 1.2899 1.3775 0.9524 0.553 0.8555 0.9989

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps52

Vps52


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps52-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available