Standard name | |
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Human Ortholog | |
Description | Htz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 5 | – | 2 | 6 | 3 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 8 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 3 | – | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 6 |
Bud Site | 1 | 0 | 1 | 0 | 1 | 6 | 7 | – | 8 | 18 | 4 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 6 | 4 | 2 | 15 | 13 | 40 | – | 11 | 23 | 14 | 7 | 13 | 23 | 38 | 7 | 6 | 3 | 0 | 1 | 0 | 0 | 1 | 10 |
Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | 1 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 1 | 0 | 0 | 6 | 4 | 0 | 4 | 7 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Mitochondria | 16 | 54 | 8 | 8 | 12 | 49 | 76 | – | 104 | 128 | 102 | 45 | 43 | 14 | 15 | 4 | 2 | 3 | 0 | 5 | 2 | 0 | 4 | 6 |
Nucleus | 102 | 187 | 115 | 30 | 104 | 82 | 108 | – | 117 | 125 | 83 | 41 | 213 | 215 | 221 | 76 | 40 | 33 | 84 | 185 | 100 | 107 | 108 | 95 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 15 | 11 | 1 | 0 | 0 | 3 | 4 | – | 8 | 5 | 5 | 2 | 18 | 16 | 19 | 3 | 4 | 4 | 22 | 24 | 21 | 25 | 46 | 47 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 2 | 12 | 0 | 6 | 9 | 11 | – | 4 | 8 | 6 | 2 | 6 | 6 | 4 | 3 | 0 | 3 | 4 | 5 | 5 | 7 | 13 | 20 |
Vac/Vac Membrane | 3 | 4 | 0 | 0 | 3 | 1 | 1 | – | 3 | 2 | 1 | 1 | 0 | 3 | 3 | 7 | 1 | 3 | 5 | 18 | 9 | 17 | 18 | 28 |
Unique Cell Count | 116 | 223 | 123 | 34 | 128 | 127 | 184 | 167 | 197 | 139 | 70 | 244 | 252 | 275 | 98 | 51 | 41 | 122 | 254 | 148 | 170 | 208 | 245 | |
Labelled Cell Count | 142 | 270 | 143 | 40 | 142 | 167 | 255 | 258 | 319 | 219 | 109 | 294 | 281 | 304 | 111 | 56 | 54 | 122 | 254 | 148 | 170 | 208 | 245 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.7 | 5.0 | 4.3 | 5.4 | 4.3 | 4.2 | – | 4.0 | 4.0 | 3.6 | 3.7 | 4.7 | 5.0 | 4.8 | 7.0 | 6.9 | 6.7 | 4.6 | 4.7 | 5.0 |
Std Deviation (1e-4) | 0.9 | 0.9 | 0.6 | 0.6 | 1.6 | 1.5 | 1.6 | – | 1.7 | 1.9 | 1.4 | 0.9 | 1.0 | 1.3 | 1.2 | 1.6 | 1.6 | 1.6 | 1.3 | 1.6 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.22 | 0.12 | -0.2 | -0.24 | – | -0.32 | -0.32 | -0.45 | -0.43 | -0.06 | 0.01 | -0.05 | 0.5 | 0.48 | 0.44 | -0.11 | -0.08 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9853 | 3.0909 | 2.917 | 2.1672 | 2.8962 | 2.7584 | 0.8031 | 0.8304 | 0.8018 | 0.2743 | 0.248 | 0.4707 | 0.9428 | 0.793 | 0.5053 | 0.7272 | 0.4187 | 0.4841 |
Actin | 0.0145 | 0 | 0.0038 | 0.0007 | 0.0059 | 0.0088 | 0.0171 | 0 | 0.0107 | 0.0005 | 0.0007 | 0.0012 | 0.0305 | 0 | 0.007 | 0.0003 | 0.0046 | 0.0052 |
Bud | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0009 | 0.0001 | 0.0006 | 0 | 0.0005 | 0.0003 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 |
Bud Neck | 0.0018 | 0.0002 | 0.0008 | 0.0001 | 0.0078 | 0.0023 | 0.0012 | 0.0001 | 0.001 | 0.0005 | 0.0006 | 0.0012 | 0.0038 | 0.0002 | 0.0002 | 0.0002 | 0.0008 | 0.0013 |
Bud Periphery | 0.0004 | 0 | 0.0006 | 0.0005 | 0.0021 | 0.0002 | 0.0012 | 0 | 0.0014 | 0.0015 | 0.0035 | 0.0002 | 0.0013 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0.0001 |
Bud Site | 0.0009 | 0.0001 | 0.0031 | 0.0003 | 0.0044 | 0.0002 | 0.002 | 0.0001 | 0.0032 | 0.0003 | 0.0004 | 0.0002 | 0.0024 | 0.0002 | 0.0006 | 0.0001 | 0.0007 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0052 | 0.0006 | 0.0018 | 0 | 0.0062 | 0.0007 | 0.0033 | 0.0015 | 0.0019 | 0.0002 | 0.0005 | 0.0003 | 0.0037 | 0.0004 | 0.0007 | 0.0001 | 0.0004 | 0.0045 |
Cytoplasmic Foci | 0.003 | 0.0001 | 0.0016 | 0.0021 | 0.0064 | 0.0014 | 0.0123 | 0 | 0.0049 | 0.0001 | 0.002 | 0.0014 | 0.0042 | 0 | 0.002 | 0.0017 | 0.0023 | 0.0016 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.0001 | 0.0012 | 0 | 0.0023 | 0.0004 | 0.0043 | 0.0001 | 0.0019 | 0.0004 | 0.0008 | 0.0002 | 0.0025 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0006 |
Endosome | 0.0143 | 0.0003 | 0.0075 | 0.0006 | 0.0202 | 0.0041 | 0.0296 | 0.0001 | 0.0174 | 0.0017 | 0.0096 | 0.0031 | 0.0168 | 0.0003 | 0.0021 | 0.0015 | 0.0297 | 0.0013 |
Golgi | 0.002 | 0 | 0.0029 | 0.0007 | 0.0102 | 0.0017 | 0.0098 | 0 | 0.0101 | 0.0003 | 0.0013 | 0.0022 | 0.0046 | 0 | 0.0015 | 0.0012 | 0.0103 | 0.001 |
Lipid Particles | 0.0075 | 0.0002 | 0.0051 | 0.0034 | 0.0198 | 0.0064 | 0.01 | 0 | 0.0114 | 0.0006 | 0.0048 | 0.0014 | 0.0066 | 0 | 0.0033 | 0.0158 | 0.0055 | 0.0063 |
Mitochondria | 0.0122 | 0.0002 | 0.0057 | 0.0355 | 0.0423 | 0.0036 | 0.0125 | 0.0002 | 0.014 | 0.0045 | 0.0127 | 0.0036 | 0.0034 | 0.0003 | 0.0064 | 0.0006 | 0.034 | 0.0029 |
None | 0.0054 | 0.0022 | 0.005 | 0 | 0.0027 | 0.0012 | 0.001 | 0.0014 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0061 | 0.0001 | 0.0018 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0242 | 0.0039 | 0.0054 | 0.0003 | 0.0371 | 0.0027 | 0.0152 | 0.0021 | 0.0052 | 0.0138 | 0.0233 | 0.0029 | 0.0112 | 0.0036 | 0.0012 | 0.0029 | 0.0017 | 0.0019 |
Nucleolus | 0.0339 | 0.0319 | 0.035 | 0.0137 | 0.0503 | 0.0664 | 0.0217 | 0.025 | 0.0181 | 0.0194 | 0.0662 | 0.0492 | 0.035 | 0.0211 | 0.0287 | 0.0176 | 0.0343 | 0.0528 |
Nucleus | 0.8246 | 0.9536 | 0.9038 | 0.9054 | 0.7327 | 0.8776 | 0.8398 | 0.9664 | 0.8855 | 0.9466 | 0.8528 | 0.9268 | 0.8455 | 0.9695 | 0.9342 | 0.9438 | 0.8668 | 0.9136 |
Peroxisomes | 0.0068 | 0 | 0.0012 | 0.0346 | 0.0134 | 0.0027 | 0.0047 | 0 | 0.0033 | 0.0001 | 0.0004 | 0.0004 | 0.0055 | 0 | 0.004 | 0.0026 | 0.0017 | 0.0012 |
Punctate Nuclear | 0.0318 | 0.0061 | 0.0129 | 0.0007 | 0.0275 | 0.0187 | 0.0056 | 0.0027 | 0.0031 | 0.0021 | 0.0121 | 0.0045 | 0.0107 | 0.0035 | 0.0047 | 0.011 | 0.002 | 0.0047 |
Vacuole | 0.0044 | 0.0004 | 0.0013 | 0.0005 | 0.004 | 0.0005 | 0.005 | 0.0001 | 0.0035 | 0.0032 | 0.0034 | 0.0006 | 0.0035 | 0.0002 | 0.0003 | 0.0001 | 0.0023 | 0.0002 |
Vacuole Periphery | 0.0024 | 0.0001 | 0.0008 | 0.0004 | 0.0033 | 0.0003 | 0.0028 | 0.0001 | 0.002 | 0.0037 | 0.0037 | 0.0003 | 0.0012 | 0.0003 | 0.0003 | 0.0001 | 0.0013 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.5516 | 7.8481 | 7.0496 | 11.4574 | 10.7835 | 9.2361 | 9.0837 | 12.2617 | 10.8882 | 11.1528 |
Translational Efficiency | 0.8579 | 1.2661 | 1.5861 | 0.9957 | 0.9849 | 1.2999 | 1.2208 | 0.9654 | 1.0966 | 1.2119 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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