Standard name | |
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Human Ortholog | |
Description | Component of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 2 | 1 | 0 | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 6 | 0 | 3 | 2 | 1 | 0 | 1 |
Bud | 4 | 9 | 39 | 5 | 35 | 28 | 48 | 59 | 33 | 55 | 42 | 64 | 3 | 3 | 6 | 10 | 3 | 11 | 0 | 2 | 0 | 0 | 1 | 0 |
Bud Neck | 2 | 1 | 18 | 0 | 17 | 10 | 4 | 9 | 3 | 8 | 4 | 7 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 0 | 0 | 0 |
Bud Site | 2 | 1 | 8 | 1 | 1 | 3 | 9 | 12 | 10 | 20 | 12 | 28 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 15 | 18 | 13 | 14 | 18 | 17 | 30 | 20 | 21 | 13 | 18 | 6 | 9 | 12 | 25 | 17 | 15 | 2 | 3 | 1 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 1 | 0 | 3 | 1 | 0 | 2 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 12 | 5 | 4 | 0 | 0 | 2 | 0 | 1 | 1 |
Endosome | 119 | 289 | 22 | 9 | 35 | 13 | 19 | 18 | 15 | 4 | 6 | 6 | 90 | 111 | 85 | 245 | 140 | 151 | 192 | 460 | 331 | 87 | 97 | 150 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 10 | 3 | 6 | 8 | 7 | 0 | 4 | 4 |
Mitochondria | 43 | 144 | 168 | 172 | 146 | 230 | 262 | 254 | 235 | 240 | 237 | 251 | 16 | 11 | 42 | 41 | 28 | 38 | 3 | 15 | 10 | 2 | 3 | 5 |
Nucleus | 0 | 6 | 1 | 2 | 1 | 2 | 2 | 4 | 0 | 3 | 2 | 4 | 0 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 28 | 2 | 2 | 3 | 3 | 3 | 0 | 3 | 3 | 3 | 2 | 1 | 0 | 3 | 13 | 4 | 9 | 1 | 2 | 1 | 0 | 0 | 0 |
Nucleolus | 34 | 78 | 19 | 11 | 10 | 18 | 14 | 19 | 18 | 13 | 10 | 13 | 15 | 20 | 16 | 24 | 11 | 27 | 0 | 7 | 3 | 0 | 0 | 0 |
Peroxisomes | 43 | 97 | 27 | 18 | 45 | 34 | 59 | 57 | 43 | 48 | 51 | 70 | 36 | 40 | 22 | 38 | 12 | 22 | 34 | 28 | 47 | 4 | 9 | 6 |
SpindlePole | 102 | 170 | 187 | 107 | 168 | 150 | 213 | 260 | 130 | 141 | 123 | 179 | 48 | 57 | 47 | 234 | 136 | 123 | 28 | 57 | 42 | 9 | 10 | 3 |
Vac/Vac Membrane | 9 | 18 | 2 | 1 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 2 | 5 | 3 | 3 | 16 | 9 | 14 | 0 | 3 | 3 | 0 | 1 | 0 |
Unique Cell Count | 238 | 555 | 374 | 268 | 359 | 374 | 469 | 512 | 365 | 383 | 357 | 410 | 135 | 160 | 165 | 460 | 261 | 269 | 275 | 604 | 462 | 111 | 134 | 178 |
Labelled Cell Count | 370 | 860 | 514 | 345 | 477 | 514 | 655 | 725 | 514 | 558 | 506 | 645 | 221 | 258 | 236 | 670 | 379 | 424 | 275 | 604 | 462 | 111 | 134 | 178 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 5.4 | 3.6 | 3.4 | 3.6 | 3.4 | 3.3 | 3.5 | 3.1 | 3.1 | 3.0 | 2.9 | 5.5 | 5.5 | 4.9 | 7.2 | 7.6 | 7.4 | 5.1 | 5.8 | 6.1 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.8 | 0.9 | 0.7 | 0.6 | 0.9 | 1.2 | 0.8 | 1.2 | 0.8 | 1.0 | 1.3 | 1.1 | 0.9 | 1.2 | 1.5 | 1.4 | 0.7 | 1.5 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.02 | -0.08 | -0.13 | -0.06 | -0.25 | -0.22 | -0.25 | -0.3 | 0.6 | 0.61 | 0.44 | 0.98 | 1.06 | 1.02 | 0.49 | 0.68 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -3.0 | -3.4 | -2.6 | -5.0 | -4.6 | -3.0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.0 | -2.9 | -4.8 | -2.9 | -3.6 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 14.6 | 15.5 | 12.2 | 14.6 | 13.6 | 14.2 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -6.9 | -8.5 | -4.3 | -11.9 | -9.2 | -8.3 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 3.0 | 2.0 | 0.1 | -0.5 | 2.4 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 5.9 | 5.7 | 2.3 | 0.6 | -1.4 | 0.5 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -2.9 | -3.1 | -4.6 | 0.2 | 0.5 | -1.1 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -0.6 | – | -0.9 | 0.7 | 0.4 | 0.4 | -0.8 | -0.3 | -0.2 | 0.4 | 1.1 | 0.4 | 0.9 | 0.4 |
Nucleus | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5737 | 2.208 | 1.7685 | 1.5807 | 1.9768 | 1.7287 | 4.3817 | 4.4063 | 4.6568 | 3.9646 | 4.1752 | 4.5738 | 4.176 | 4.6187 | 4.4631 | 4.5856 | 4.2297 | 4.9115 |
Actin | 0.009 | 0.0018 | 0.0099 | 0 | 0.0188 | 0.0005 | 0.0113 | 0.0009 | 0.0064 | 0.0001 | 0.0003 | 0.0007 | 0.0097 | 0.0002 | 0.0009 | 0.0008 | 0 | 0.0036 |
Bud | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0005 | 0.0012 | 0.0002 | 0.0005 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0011 | 0.0004 | 0.0005 | 0.0001 | 0.0033 | 0.001 | 0.0006 | 0.0026 | 0.0011 | 0.0001 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0.0001 |
Bud Periphery | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0006 | 0.0007 | 0.0002 | 0.0004 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Bud Site | 0.001 | 0.0024 | 0.0006 | 0.0001 | 0.0026 | 0.0004 | 0.0033 | 0.0042 | 0.0147 | 0 | 0.0005 | 0.0002 | 0.0006 | 0.0002 | 0.0007 | 0.0006 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0029 | 0.001 | 0.0013 | 0.0004 | 0.0026 | 0.002 | 0.0135 | 0.007 | 0.0113 | 0.0004 | 0.0015 | 0.0031 | 0.0011 | 0.0004 | 0.0005 | 0.0006 | 0 | 0.0002 |
Cytoplasmic Foci | 0.1964 | 0.2672 | 0.2245 | 0.181 | 0.2097 | 0.1868 | 0.1655 | 0.2112 | 0.1558 | 0.1855 | 0.1436 | 0.1477 | 0.2021 | 0.2049 | 0.1789 | 0.1791 | 0.2389 | 0.1566 |
Eisosomes | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0015 | 0.0001 | 0.0006 | 0.0001 | 0.0019 | 0.0001 | 0.0016 | 0.0004 | 0.0017 | 0.0001 | 0.0006 | 0.0006 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endosome | 0.2234 | 0.186 | 0.1892 | 0.2755 | 0.2022 | 0.1643 | 0.2724 | 0.31 | 0.2651 | 0.3269 | 0.3608 | 0.2119 | 0.1409 | 0.1258 | 0.1396 | 0.1424 | 0.0463 | 0.0857 |
Golgi | 0.0446 | 0.048 | 0.0637 | 0.0578 | 0.0637 | 0.0453 | 0.0496 | 0.0621 | 0.0665 | 0.0828 | 0.07 | 0.0603 | 0.0484 | 0.0485 | 0.0546 | 0.0856 | 0.0175 | 0.0375 |
Lipid Particles | 0.3477 | 0.2664 | 0.2743 | 0.3112 | 0.2716 | 0.3702 | 0.2443 | 0.1744 | 0.1837 | 0.2058 | 0.1761 | 0.3172 | 0.2813 | 0.2931 | 0.2541 | 0.2248 | 0.3652 | 0.4297 |
Mitochondria | 0.0062 | 0.0043 | 0.0287 | 0.0012 | 0.025 | 0.0076 | 0.0037 | 0.0097 | 0.0124 | 0.0012 | 0.0089 | 0.0065 | 0.0039 | 0.002 | 0.0177 | 0.0151 | 0.0001 | 0.0035 |
None | 0.008 | 0.0002 | 0.0006 | 0.0001 | 0.0006 | 0.0018 | 0.0056 | 0.0039 | 0.0102 | 0.0001 | 0.0002 | 0.0019 | 0.001 | 0.0259 | 0.0128 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0069 | 0.0002 | 0.0019 | 0.0001 | 0.0137 | 0.0006 | 0.0055 | 0.0004 | 0.0005 | 0.0001 | 0.0003 | 0.0005 | 0.0006 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 |
Nucleolus | 0.002 | 0.0009 | 0.0002 | 0.0001 | 0.0008 | 0.0005 | 0.0011 | 0.0018 | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0023 | 0.0002 | 0.0005 | 0 | 0.0022 | 0.0003 | 0.0117 | 0.0009 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 |
Peroxisomes | 0.1323 | 0.201 | 0.1885 | 0.1695 | 0.1669 | 0.2097 | 0.1925 | 0.1848 | 0.2565 | 0.1939 | 0.2236 | 0.2421 | 0.3026 | 0.2954 | 0.3348 | 0.3476 | 0.3295 | 0.2758 |
Punctate Nuclear | 0.0084 | 0.0089 | 0.0113 | 0.0007 | 0.0026 | 0.0039 | 0.0118 | 0.005 | 0.0025 | 0.0008 | 0.0008 | 0.002 | 0.0045 | 0.0011 | 0.002 | 0.0008 | 0.0019 | 0.0062 |
Vacuole | 0.0037 | 0.0095 | 0.0014 | 0.0015 | 0.0062 | 0.0017 | 0.0044 | 0.0168 | 0.0064 | 0.0015 | 0.0085 | 0.003 | 0.0011 | 0.001 | 0.0009 | 0.0007 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.002 | 0.001 | 0.0009 | 0.0008 | 0.004 | 0.0009 | 0.0013 | 0.0028 | 0.0022 | 0.0007 | 0.0029 | 0.0014 | 0.0007 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.7236 | 5.1983 | 6.5093 | 8.7017 | 6.1258 | 5.4486 | 7.9661 | 10.7532 | 9.3165 | 10.1056 |
Translational Efficiency | 0.856 | 0.8379 | 0.7494 | 0.7717 | 0.848 | 1.1313 | 0.6464 | 0.5551 | 0.7059 | 0.6931 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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