Standard name
Human Ortholog
Description Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.07 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.11 0 0.13 0 0.12 0.1 0.05 0.13 0.13 0.14 0.12 – 0.15 0.13 0.11 0.14 0.08 0 0 0 0 0 0
Nucleus 0.46 0.42 0.23 0 0.09 0.1 0.1 0.09 0.08 0.14 0.12 0.1 – 0.07 0.08 0.21 0.17 0.24 0.27 0.23 0.24 0.31 0.18 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.61 0.61 0.81 0.94 0.88 0.85 0.85 0.84 0.8 0.76 0.8 0.85 – 0.83 0.82 0.7 0.74 0.72 0.67 0.67 0.69 0.62 0.66 0.55
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0.07 0.08 0.11 0.09 0.1 0.08 0.09 – 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0.06 0.06 0 0.05 0.05 0.06 0.1 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 0 1 1 1 2 – 0 0 0 0 0 1 1 0 0 0 2
Bud Neck 0 0 0 1 0 1 0 2 0 1 0 2 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 2 2 4 6 6 – 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 2 3 8 7 7 16 8 10 5 5 – 3 10 10 16 14 0 0 0 0 0 5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 3 7 5 0 0 0 0 0 0
Endosome 0 0 0 2 6 14 22 25 12 20 14 21 – 1 1 2 2 0 0 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 23 17 3 20 13 37 53 31 45 50 56 59 – 11 18 28 47 29 0 0 0 0 2 4
Nucleus 83 62 20 4 30 33 52 62 28 53 45 47 – 5 11 53 60 82 50 39 23 4 17 22
Nuclear Periphery 2 0 1 1 1 2 3 3 4 0 3 1 – 0 0 0 0 0 7 0 0 0 0 0
Nucleolus 111 90 71 141 295 270 434 554 270 295 314 403 – 59 117 173 256 248 124 114 67 8 64 74
Peroxisomes 0 1 0 3 5 4 9 8 10 5 3 4 – 0 2 1 1 2 0 0 0 0 0 0
SpindlePole 1 0 1 5 18 22 39 73 32 39 32 43 – 1 7 0 2 2 1 1 0 0 0 2
Vac/Vac Membrane 1 6 0 0 10 4 7 10 9 6 5 8 – 1 1 15 19 19 2 9 4 0 10 17
Unique Cell Count 181 148 88 150 336 318 508 656 339 390 391 473 71 142 248 344 345 187 169 98 14 98 135
Labelled Cell Count 223 179 98 180 386 394 629 786 422 484 484 601 81 169 285 410 401 187 169 98 14 98 135


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.0 31.8 17.9 25.3 21.2 17.0 15.6 15.4 14.3 13.4 12.7 13.0 – 28.2 25.2 49.0 51.6 45.0 – 25.2 25.3
Std Deviation (1e-4) 7.3 8.7 5.8 7.6 6.1 5.6 4.5 4.5 4.5 4.1 3.8 4.0 – 9.5 10.2 21.2 23.1 20.7 – 7.8 9.9
Intensity Change (Log2) – – – 0.5 0.24 -0.08 -0.2 -0.22 -0.33 -0.42 -0.49 -0.47 – 0.65 0.49 1.45 1.53 1.33 – 0.49 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 2.5 0 2.3 2.1 0 2.6 2.5 2.8 2.5 – 2.7 2.4 2.2 2.7 1.6
Nucleus -5.0 -3.6 -3.0 -3.3 -3.7 -3.8 -2.2 -2.8 -3.4 – -2.7 -3.2 -0.3 -1.1 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 3.2 1.7 1.0 1.1 0.9 -0.2 -1.0 -0.1 1.1 – 0.4 0.3 -2.0 -1.2 -1.7
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 2.3 2.9 2.6 2.7 2.4 2.5 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.0527 29.8703 25.797 24.9371 17.9406 24.9871 26.4497 31.0149 27.098 25.6513 24.7382 26.1274 20.3076 26.4677 23.8489 22.0983 20.025 22.8071
Actin 0.0112 0 0.0057 0.0001 0.002 0.0015 0.0252 0 0.0076 0.0001 0.0294 0.0016 0.0103 0 0 0.043 0.0001 0.0087
Bud 0.0003 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0004 0.0002 0.0002 0.0003 0 0 0.0001 0.0001 0.0001
Bud Neck 0.0008 0 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0004 0.0003 0.0002 0.0006 0.0001 0 0.0002 0.0001 0.0001
Bud Periphery 0.0004 0 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0004 0.0005 0.0006 0.0003 0 0 0.0001 0.0002 0.0002
Bud Site 0.0039 0 0.0005 0.0001 0.0002 0.0001 0.0004 0 0.0002 0.0065 0.0005 0.0003 0.0072 0 0 0.0005 0.0001 0.0001
Cell Periphery 0.0005 0.0001 0.0004 0.0004 0.0006 0.0009 0.0003 0.0013 0.0002 0.0006 0.0023 0.001 0.0003 0.0002 0.0001 0.0003 0.0009 0.0007
Cytoplasm 0.0005 0 0.0002 0.0011 0.0005 0 0.0025 0 0.0008 0.0025 0.0008 0.0001 0.0034 0 0 0 0.0001 0
Cytoplasmic Foci 0.0105 0.0001 0.0103 0.0028 0.0074 0.001 0.0064 0.0001 0.0066 0.0056 0.0153 0.0045 0.0169 0 0 0.0067 0.0208 0.0005
Eisosomes 0.0003 0 0.0002 0.0001 0.0019 0.0001 0.0003 0 0 0 0.0003 0.0001 0.0001 0 0 0.0004 0.0002 0.0001
Endoplasmic Reticulum 0.0007 0 0.0003 0.0004 0.0001 0.0001 0.0013 0 0.0002 0.0005 0.0002 0.0001 0.0012 0 0 0 0 0.0001
Endosome 0.008 0 0.0019 0.0007 0.0007 0.0009 0.0034 0 0.0062 0.0179 0.0018 0.0037 0.0072 0 0 0.0001 0.0001 0.0012
Golgi 0.0034 0 0.0018 0.0001 0.0009 0.0013 0.0015 0 0.0042 0.0015 0.0042 0.0031 0.0027 0 0 0.0007 0.0002 0.0016
Lipid Particles 0.0087 0.0001 0.0087 0.0028 0.0326 0.0055 0.0067 0.0005 0.0029 0.0031 0.0184 0.0117 0.0096 0.0001 0.0001 0.0035 0.0111 0.008
Mitochondria 0.0151 0.0004 0.0109 0.0371 0.014 0.0318 0.0059 0.0176 0.0023 0.0039 0.0334 0.0531 0.0024 0.0004 0.0004 0.0341 0.0166 0.0173
None 0.0018 0 0.0011 0.0011 0.0197 0.0001 0.0258 0 0.0005 0.0005 0.0004 0.0002 0.0042 0 0 0.0006 0.0009 0.0001
Nuclear Periphery 0.0101 0.0001 0.0044 0.023 0.0529 0.0222 0.0082 0.0002 0.0029 0.0034 0.0411 0.0145 0.0086 0.0016 0.0008 0.0009 0.0158 0.0138
Nucleolus 0.8461 0.9043 0.893 0.8553 0.7601 0.8439 0.819 0.893 0.8819 0.8036 0.6899 0.7576 0.8397 0.8449 0.8859 0.7806 0.7578 0.7987
Nucleus 0.0652 0.0941 0.0493 0.0621 0.0599 0.0722 0.087 0.0824 0.0738 0.1311 0.1066 0.1059 0.066 0.1513 0.1119 0.1201 0.1515 0.1226
Peroxisomes 0.0043 0 0.0065 0.0007 0.0232 0.0033 0.0012 0 0.0042 0.0007 0.0082 0.0055 0.0068 0 0 0.0064 0.0009 0.0004
Punctate Nuclear 0.0046 0.0001 0.0012 0.005 0.0154 0.0005 0.002 0 0.0025 0.0008 0.0017 0.0151 0.006 0.0001 0.0001 0.0001 0.0022 0.0022
Vacuole 0.0015 0.0002 0.0008 0.0013 0.001 0.0017 0.0009 0.0017 0.0012 0.0099 0.0063 0.0017 0.0012 0.0003 0.0001 0.0006 0.002 0.002
Vacuole Periphery 0.002 0.0003 0.0023 0.0056 0.0064 0.0125 0.0017 0.0029 0.0012 0.0067 0.0382 0.0193 0.0049 0.0011 0.0004 0.0009 0.0182 0.0215

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 80.614 83.5742 57.5595 59.1719 62.0585 61.5461 51.4854 40.9818 40.0212 64.3472
Translational Efficiency 1.9486 1.7199 2.1297 1.513 1.8 1.5754 1.5579 1.4103 1.3662 1.3864

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis
Localization
Cell Percentages nucleolus (38%), nucleus (17%), mixed (39%)
Cell Cycle Regulation Yes
nucleus - MA (2.9e-08)
Subcompartmental Group N/A

Puf6

Puf6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Puf6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available