Standard name
Human Ortholog
Description Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.07 0.12
Bud 0 0 0.07 0.07 0.16 0.16 0.1 0.12 0.15 0.1 0.1 0.1 0.07 0 0.09 0.06 0 0 0.26 0.25 0.27 0.13 0 0.07
Bud Neck 0.18 0.06 0.21 0.14 0.25 0.15 0.17 0.18 0.15 0.13 0.11 0.1 0.17 0.16 0.2 0.24 0.19 0.14 0.34 0.16 0.29 0.19 0.07 0.12
Bud Site 0.13 0.11 0.1 0.16 0.16 0.14 0.09 0.05 0.08 0.05 0 0.07 0.13 0.17 0.14 0.11 0 0.05 – – – – – –
Cell Periphery 0.09 0 0 0 0 0 0 0 0 0 0.05 0.07 0.08 0.05 0.11 0.14 0.08 0.09 0 0 0 0 0 0.05
Cytoplasm 0.49 0.61 0.52 0.47 0.35 0.39 0.52 0.48 0.53 0.55 0.6 0.57 0.47 0.41 0.41 0.39 0.65 0.53 0.27 0.44 0.26 0.55 0.72 0.51
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.08 0.13 0.05 0.11 0.1 0.15 0.24 0.27 0.27 0.33 0 0 0 0.1 0 0.06 0 0 0 0 0 0
Nucleus 0.06 0 0.06 0.09 0.06 0.1 0.06 0.07 0 0.05 0 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.06 0.12 0 0.09 0.11 0.08 0.13 0.1 0.11 0.06 0.07 0.11 0.11 0.09 0.18 0.14 0.15 0 0 0 0 0 0
Peroxisomes 0 0.06 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.11 0 0 0.1 0.15 0.17 0.15 0.12 0.11 0.08 0.06 0.08 0.12 0.13 0.1 0.07 0.08 0.05 0.06 0 0.06 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 0 2 4 3 5 2 5 0 2 3 1 4 3 4 2 0 0 2 0 0 0 12 23
Bud 13 0 7 11 48 40 42 66 40 36 24 31 5 5 12 5 0 0 100 13 30 9 6 13
Bud Neck 59 1 20 21 76 38 71 97 42 49 28 32 13 19 27 20 7 11 131 8 33 12 12 22
Bud Site 41 2 10 24 50 35 39 28 22 17 8 23 10 21 19 9 1 4 – – – – – –
Cell Periphery 28 0 0 6 6 9 7 6 12 11 12 22 6 6 14 12 3 7 1 0 0 0 2 9
Cytoplasm 158 11 50 71 107 96 217 254 145 207 149 180 35 49 54 32 24 43 102 23 29 38 124 98
Endoplasmic Reticulum 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 3 1 0 0 1 1 3
Endosome 6 0 2 1 6 4 5 5 0 0 0 0 2 6 2 1 1 3 3 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 1
Mitochondria 1 1 8 20 15 27 43 79 67 103 67 103 1 2 4 8 0 5 1 0 1 2 1 5
Nucleus 19 0 6 13 18 25 24 39 12 20 9 6 1 7 11 0 1 2 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 2 2 1 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 42 1 12 5 27 26 33 70 28 40 16 21 8 13 12 15 5 12 3 0 3 0 0 0
Peroxisomes 3 1 2 3 13 7 4 4 4 6 2 2 8 8 5 2 0 2 1 0 1 0 0 0
SpindlePole 36 0 4 15 45 43 65 64 29 31 15 25 9 16 13 6 3 4 21 1 7 0 0 0
Vac/Vac Membrane 7 1 0 1 3 4 2 10 2 1 5 5 1 0 0 5 1 3 7 1 1 1 3 5
Unique Cell Count 322 18 96 150 309 246 421 534 275 375 250 316 75 120 132 83 37 81 383 54 115 69 172 192
Labelled Cell Count 418 19 123 195 417 359 556 730 404 523 339 451 104 155 177 119 46 100 383 54 115 69 172 192


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 6.1 5.2 5.1 5.5 5.0 4.7 4.7 4.2 4.1 4.0 3.7 8.0 8.1 7.2 12.1 11.4 14.6 6.8 7.2 6.6
Std Deviation (1e-4) 2.9 1.4 1.3 1.2 1.4 1.3 1.4 1.5 1.1 1.4 1.3 1.3 2.4 2.4 2.2 3.0 4.7 5.8 1.2 7.5 1.6
Intensity Change (Log2) – – – -0.01 0.08 -0.05 -0.15 -0.14 -0.3 -0.34 -0.37 -0.47 0.63 0.66 0.49 1.22 1.14 1.5 0.39 0.47 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2.1 2.2 0 0 0 0 0 0 -0.2 -1.0 0.5 -0.3 0 0
Bud Neck -1.4 0.8 -1.2 0 0 0 0 0 0 -0.6 -0.9 -0.1 0.5 -0.2 -1.3
Bud Site 1.2 1.4 0.9 0 0 0 0 0 0 0.6 1.5 0.9 0.1 0 -1.3
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 3.3 3.9 0 0
Cytoplasm -0.7 -3.1 -2.2 -0.1 -0.8 0.1 0.5 1.3 0.8 -0.7 -1.6 -1.7 -1.8 1.3 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.2 -1.3 0.7 0 0 0 0 0 0 0 0 -1.8 0.3 0 -0.5
Nucleus 0.7 -0.2 1.1 -0.2 0.4 0 -0.4 0 0 0 -0.1 0.6 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.8 -1.1 -0.5 0 0 0 0 0 0 -0.4 -0.4 -0.8 1.0 0 0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.7 2.7 3.2 0 0 0 0 0 0 1.9 2.3 1.6 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3191 5.053 5.2925 5.0205 4.2327 4.4829 4.5631 6.928 8.1346 6.9925 6.1684 6.6168 2.4267 4.7324 5.2301 5.104 4.9833 3.6826
Actin 0.0053 0.0008 0.001 0.0001 0.0002 0.0103 0.0731 0.0173 0.032 0.0529 0.0274 0.015 0.0102 0.0015 0.0067 0.0116 0.0002 0.0063
Bud 0.0289 0.0257 0.0083 0.0002 0.0019 0.0207 0.017 0.033 0.0092 0.0006 0.0021 0.0228 0.0238 0.0407 0.0075 0.0007 0.0012 0.0381
Bud Neck 0.0457 0.0409 0.6618 0.9053 0.9193 0.4683 0.0382 0.0244 0.5757 0.8695 0.4883 0.4191 0.0571 0.0287 0.6881 0.953 0.9163 0.4249
Bud Periphery 0.0182 0.0291 0.0087 0.0002 0.0023 0.0207 0.0119 0.0262 0.0052 0.0008 0.0038 0.033 0.0176 0.0184 0.0074 0.0008 0.0017 0.0411
Bud Site 0.4261 0.7021 0.2879 0.0048 0.0028 0.0433 0.2494 0.6486 0.2874 0.0025 0.018 0.0226 0.4617 0.6388 0.1984 0.0037 0.0255 0.0278
Cell Periphery 0.0087 0.0055 0.0033 0.0009 0.0014 0.0052 0.0068 0.0089 0.0042 0.001 0.0017 0.002 0.0067 0.0083 0.0031 0.0006 0.0014 0.0053
Cytoplasm 0.2281 0.0855 0.0003 0.0439 0.0004 0.3054 0.2412 0.1632 0.0098 0.0011 0.0197 0.3886 0.1697 0.073 0.0006 0.003 0.0001 0.3318
Cytoplasmic Foci 0.1161 0.027 0.0076 0.0035 0.0131 0.074 0.1224 0.0316 0.0406 0.0279 0.0651 0.0323 0.0892 0.0436 0.0239 0.0023 0.0246 0.0526
Eisosomes 0.0004 0 0 0 0 0.0001 0.0005 0.0001 0.0003 0.0003 0.0011 0.0001 0.0003 0.0004 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0054 0.0003 0 0.0003 0 0.0012 0.0149 0.0008 0.0001 0.0012 0.0054 0.0013 0.0048 0.0006 0.0001 0.0004 0 0.0017
Endosome 0.0013 0.0008 0.0001 0.0012 0.0005 0.0015 0.0634 0.001 0.0025 0.008 0.1115 0.0038 0.0148 0.0011 0.0016 0.0013 0.0002 0.0043
Golgi 0.0009 0.0002 0.0002 0.0003 0.0006 0.0013 0.0319 0.0011 0.0039 0.015 0.0689 0.0033 0.0049 0.0003 0.0016 0.0006 0.0003 0.0015
Lipid Particles 0.0064 0.0005 0.0005 0.0003 0.0038 0.0028 0.0439 0.0009 0.0044 0.0032 0.0244 0.0068 0.0115 0.0007 0.0065 0.0002 0.0152 0.0042
Mitochondria 0.0016 0.0034 0.0002 0.0001 0.0006 0.0005 0.0073 0.0012 0.0042 0.0008 0.0513 0.0045 0.0127 0.0014 0.0066 0.0003 0.001 0.0049
None 0.071 0.0024 0.0001 0.0002 0 0.0088 0.0112 0.002 0.0003 0.0002 0.0087 0.0157 0.0369 0.0162 0.0004 0.0013 0.0001 0.0241
Nuclear Periphery 0.0005 0.0014 0 0 0 0.0003 0.0207 0.0011 0.0001 0.0002 0.0088 0.0003 0.0017 0.0007 0.0003 0.0005 0 0.0007
Nucleolus 0.013 0.0165 0.0122 0.034 0.0423 0.0065 0.0045 0.0109 0.0094 0.0037 0.0113 0.0214 0.0272 0.0307 0.0219 0.0144 0.007 0.0047
Nucleus 0.0026 0.0378 0.0033 0.0029 0.0042 0.0032 0.0041 0.0069 0.0022 0.0008 0.0065 0.0013 0.0044 0.037 0.0033 0.0025 0.0018 0.0048
Peroxisomes 0.0056 0.0007 0.0004 0.0001 0.0037 0.003 0.0141 0.0011 0.0049 0.0063 0.0111 0.0026 0.0181 0.0031 0.0139 0.0007 0.0017 0.0026
Punctate Nuclear 0.0088 0.0092 0.0024 0.0006 0.0011 0.0198 0.0112 0.0029 0.001 0.0033 0.0488 0.0015 0.0155 0.0092 0.0061 0.002 0.0009 0.0076
Vacuole 0.0052 0.0085 0.0016 0.0009 0.0015 0.003 0.0089 0.0159 0.0023 0.0005 0.0113 0.0018 0.0104 0.043 0.0018 0.0002 0.0006 0.0101
Vacuole Periphery 0.0003 0.0016 0.0001 0 0.0001 0.0002 0.0033 0.0008 0.0002 0.0001 0.0046 0.0004 0.001 0.0026 0.0002 0 0.0001 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 78.5158 28.7657 19.6378 40.0398 71.5515 80.402 29.0123 21.986 32.3802 77.2415
Translational Efficiency 1.5588 1.5435 0.9404 1.0186 1.2272 1.569 1.1018 0.8093 0.8441 1.0614

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication
Localization
Cell Percentages bud neck (34%), cytoplasm (24%), mixed (17%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (5e-08)
bud neck - S/G2 (5.2e-06)
bud neck - MA (6.3e-05)
Subcompartmental Group N/A

Gin4

Gin4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gin4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available