Standard name
Human Ortholog
Description Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; human homolog SUMO1 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 1.0 0.99 0.99 – 0.9 0.92 0.84 0.85 0.83 1.0 0.99 1.0 0.96 0.95 0.96 0.97 0.95 0.94 0.89 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.07 0 0.06 0.06 0.07 0 0 0 0.08 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 – 0 4 6 5 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 3 0 0 0 – 1 0 0 2 6 1 2 1 0 0 0 1 0 0 0 2 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 0 0 3 1 – 5 4 14 15 8 0 3 4 0 2 2 0 0 0 0 0 0
Cytoplasm 371 274 120 253 427 380 – 430 339 417 315 388 233 399 451 47 79 85 361 260 112 87 314 294
Endoplasmic Reticulum 1 2 0 0 5 3 – 35 12 30 21 32 1 0 2 4 3 2 1 0 0 0 2 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 2 2 0 1 0 1
Golgi 0 2 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 1 0 2 0 0 0 2
Mitochondria 3 1 0 0 0 0 – 1 5 11 14 24 0 1 0 0 1 1 0 0 0 0 1 0
Nucleus 1 0 0 0 0 0 – 7 3 3 3 4 0 0 0 0 1 0 1 2 3 3 9 6
Nuclear Periphery 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 1 2 1 – 10 10 32 12 25 0 3 0 1 1 2 0 3 1 3 4 4
Unique Cell Count 375 279 123 254 432 384 476 369 495 370 467 234 402 453 49 83 89 373 274 120 98 340 323
Labelled Cell Count 379 281 123 254 438 385 490 378 514 388 490 235 408 458 53 88 93 373 274 120 98 340 323


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 37.1 40.1 18.4 25.8 28.2 26.5 – 23.9 23.8 21.6 21.0 21.1 40.4 44.2 45.1 53.0 54.7 56.7 34.9 39.5 39.3
Std Deviation (1e-4) 7.7 8.0 4.7 5.8 5.3 5.0 – 4.9 4.7 5.1 4.8 4.9 9.1 11.1 9.4 24.0 20.2 20.6 9.6 9.1 10.5
Intensity Change (Log2) – – – 0.48 0.61 0.52 – 0.38 0.37 0.23 0.19 0.19 1.13 1.26 1.29 1.52 1.57 1.62 0.92 1.1 1.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 1.1 1.2 – -2.6 -2.2 -3.9 -3.7 -4.1 1.7 1.5 2.1 -0.6 -0.9 -0.8
Endoplasmic Reticulum 0 0 0 – 3.1 0 2.8 2.7 3.0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 2.6 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 2.9 0 2.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 30.7998 40.8844 38.3048 34.5777 33.9769 37.8696 29.9527 43.0973 38.6484 36.4793 38.8886 39.4928 37.2715 47.8885 45.5664 42.1826 42.1793 48.3168
Actin 0.0158 0.0002 0.0002 0.0187 0.0007 0.0015 0.0164 0.0001 0.009 0.0182 0.0261 0.0001 0.0053 0.0001 0.0075 0.0046 0.0043 0.0001
Bud 0.0011 0 0.0002 0.0005 0.0004 0.0001 0.001 0 0.0008 0.0004 0.0011 0 0.0004 0 0.0001 0.0001 0.0017 0
Bud Neck 0.0004 0.0001 0.0002 0.001 0.0003 0.0004 0.0004 0.0001 0.0007 0.0004 0.0002 0.0003 0.0003 0.0001 0.0002 0.0002 0.0073 0.0003
Bud Periphery 0.0009 0 0.0001 0.0003 0.0007 0.0001 0.0006 0 0.0004 0.0004 0.0022 0 0.0003 0 0 0.0001 0.0018 0
Bud Site 0.001 0 0 0.0009 0.0005 0.0001 0.0017 0 0.0012 0.0012 0.0003 0 0.0007 0 0.0001 0.0007 0.0471 0
Cell Periphery 0.0003 0 0 0.0001 0.0001 0 0.0002 0 0 0.0002 0.0001 0 0.0001 0 0 0.0001 0.0004 0
Cytoplasm 0.854 0.9927 0.9858 0.8947 0.8578 0.9406 0.8281 0.9955 0.9416 0.8439 0.9233 0.9902 0.8507 0.9947 0.9687 0.9233 0.9064 0.9714
Cytoplasmic Foci 0.0168 0.0002 0.0003 0.0184 0.007 0.0005 0.0124 0.0001 0.0043 0.0056 0.0006 0.0002 0.0289 0.0001 0.0012 0.0208 0.0061 0.0002
Eisosomes 0.0002 0 0 0 0 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.008 0.0002 0.0003 0.0021 0.0008 0.0007 0.0151 0.0001 0.001 0.0106 0.0014 0.0002 0.0029 0.0001 0.0002 0.0037 0.0004 0.0004
Endosome 0.0129 0 0.0001 0.0024 0.0496 0.0049 0.0319 0 0.0091 0.0426 0.0091 0.0001 0.0109 0 0.001 0.0038 0.0044 0
Golgi 0.0036 0 0 0.0022 0.0085 0.0009 0.0111 0 0.002 0.0222 0.0107 0 0.0035 0 0.0027 0.0018 0.002 0
Lipid Particles 0.0048 0 0 0.0024 0.0077 0.001 0.0105 0 0.0091 0.0028 0.0003 0 0.0155 0 0.0041 0.021 0.0015 0
Mitochondria 0.0058 0 0.0001 0.002 0.043 0.0165 0.0093 0 0.0025 0.009 0.0164 0 0.0086 0 0.0015 0.0021 0.0006 0
None 0.0041 0.0002 0.0003 0.0047 0.0007 0.0003 0.0021 0.0001 0.0008 0.0011 0.0003 0.0001 0.0035 0.0001 0.0002 0.0036 0.0005 0.0001
Nuclear Periphery 0.0217 0.0008 0.0014 0.0061 0.0021 0.0133 0.0134 0.0005 0.0027 0.0216 0.0016 0.0007 0.0059 0.0004 0.0007 0.0022 0.0012 0.0024
Nucleolus 0.0006 0 0 0.0012 0.0002 0.0001 0.0004 0 0.0001 0.0002 0 0 0.0024 0 0 0.0002 0.0006 0
Nucleus 0.033 0.0055 0.0108 0.013 0.0082 0.0172 0.0189 0.0032 0.0096 0.0145 0.0049 0.008 0.0279 0.0041 0.0074 0.0061 0.0094 0.0248
Peroxisomes 0.0053 0 0 0.0168 0.0026 0.0002 0.006 0 0.0023 0.0005 0.0001 0 0.0055 0 0.0041 0.0022 0.0017 0
Punctate Nuclear 0.005 0.0001 0.0001 0.0096 0.0004 0.0006 0.0102 0.0001 0.0004 0.001 0.0001 0 0.0239 0.0001 0.0001 0.0023 0.0006 0.0001
Vacuole 0.0029 0.0001 0.0002 0.0019 0.0043 0.0004 0.0064 0 0.0013 0.0018 0.0004 0.0001 0.0019 0 0.0001 0.0006 0.0019 0.0001
Vacuole Periphery 0.002 0 0 0.0011 0.0045 0.0007 0.0037 0 0.0012 0.0018 0.0008 0 0.0009 0 0 0.0005 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 239.7921 332.0155 267.8973 314.8872 247.7858 313.5394 351.1819 411.355 401.3157 292.3539
Translational Efficiency 3.5867 2.8707 3.0148 2.9212 3.6002 3.2997 2.8296 2.7149 2.5363 2.6693

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; human homolog SUMO1 can complement yeast null mutant
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Smt3

Smt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available