Standard name
Human Ortholog
Description Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.93 0.96 0.99 0.97 0.93 0.87 0.76 0.67 0.69 0.65 0.61 0.6 1.0 0.99 0.99 0.98 0.95 0.92 0.88 0.88 0.87 0.93 0.92 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.06 0.11 0.24 0.31 0.29 0.36 0.4 0.5 0.5 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.14 0 0.08 0 0.06 0 0 0 0 0.05 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 1 0 1 0 0 0 0 0 1 2 4 3 0 1 2
Bud 0 4 4 0 0 3 4 12 5 7 2 6 0 1 0 0 0 0 1 5 1 0 1 4
Bud Neck 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 1 2
Bud Site 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 4 6 6 6 10 8 21 12 10 24 0 5 4 2 9 4 0 1 0 0 0 0
Cytoplasm 280 365 310 259 271 356 305 392 312 248 208 250 129 322 288 202 173 149 272 344 264 101 182 198
Endoplasmic Reticulum 2 6 0 0 1 1 4 9 10 6 7 1 0 0 2 9 10 4 0 0 3 0 1 2
Endosome 0 1 0 1 1 0 0 1 0 2 1 0 0 0 0 2 1 2 3 5 2 0 0 0
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 4 0 0 2 0
Mitochondria 26 6 9 17 32 100 122 172 164 152 169 206 0 0 0 2 3 4 9 8 15 1 2 3
Nucleus 0 0 0 0 1 2 2 6 8 2 1 2 0 0 1 1 0 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 0 4 4 3 5 4 4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2
SpindlePole 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 2 3 1 0 1 0
Vac/Vac Membrane 3 2 0 1 10 15 25 81 15 29 14 24 0 3 1 4 10 10 5 2 2 1 0 6
Unique Cell Count 301 382 314 266 291 410 399 589 451 379 341 414 129 325 291 207 183 162 309 389 303 109 198 230
Labelled Cell Count 312 390 327 285 323 484 477 686 541 464 416 518 129 331 296 225 206 176 309 389 303 109 198 230


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.9 4.2 4.3 5.3 5.4 5.9 5.7 6.3 6.1 6.1 6.3 6.3 8.2 8.7 7.1 7.9 7.6 4.2 4.6 5.2
Std Deviation (1e-4) 0.7 1.3 1.1 1.5 1.9 2.0 2.6 2.0 2.4 2.5 2.2 2.6 2.6 3.1 3.2 2.8 2.7 2.6 1.3 1.5 1.4
Intensity Change (Log2) – – – 0.03 0.32 0.35 0.48 0.43 0.58 0.52 0.53 0.56 0.56 0.95 1.04 0.74 0.9 0.83 -0.01 0.12 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.8 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.2 0 2.6 1.7 1.5 3.1 0 0 0 0 0 0
Cytoplasm -1.2 -3.5 -5.8 -8.6 -11.1 -10.3 -11.0 -11.9 -12.2 1.3 0.4 0.3 -1.0 -2.7 -3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.0 8.0 9.5 9.4 10.9 11.6 13.4 13.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.4 4.5 6.9 3.3 5.0 3.6 4.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2922 4.2406 3.7741 2.9508 2.8944 3.9037 5.6449 5.2361 6.1079 5.9671 6.7342 5.4954 6.2988 6.8667 6.2256 6.1958 6.5673 7.3556
Actin 0.0366 0.0008 0.0033 0.0103 0.0028 0.0065 0.0508 0.0031 0.0213 0.0126 0.0325 0.0189 0.0132 0.0034 0.0362 0.0042 0.0372 0.0106
Bud 0.0037 0.0064 0.0054 0.0004 0.0042 0.0004 0.0017 0.0019 0.0034 0.0007 0.0018 0.005 0.0012 0.0019 0.0059 0.0025 0.0119 0.0031
Bud Neck 0.0124 0.0002 0.0003 0.0007 0.0007 0.001 0.0019 0.0004 0.0008 0.0015 0.0024 0.0012 0.0033 0.0003 0.0055 0.0004 0.0014 0.0018
Bud Periphery 0.0086 0.0045 0.005 0.0006 0.0067 0.0007 0.0027 0.0034 0.0055 0.0008 0.0033 0.0025 0.0014 0.0012 0.0069 0.0041 0.0179 0.0064
Bud Site 0.0046 0.0044 0.0065 0.0016 0.0005 0.0006 0.0047 0.0056 0.022 0.0021 0.0025 0.0006 0.0023 0.0082 0.0208 0.0015 0.0025 0.0007
Cell Periphery 0.0009 0.0006 0.0004 0.0003 0.0001 0.0001 0.0004 0.0005 0.0007 0.0003 0.0002 0.0002 0.0002 0.0003 0.0004 0.0003 0.0003 0.0001
Cytoplasm 0.6023 0.7484 0.6265 0.6534 0.6091 0.7462 0.6244 0.7032 0.7117 0.5928 0.6631 0.7485 0.6785 0.8104 0.6508 0.609 0.6075 0.763
Cytoplasmic Foci 0.0235 0.0076 0.0125 0.0484 0.0189 0.0138 0.039 0.0065 0.0114 0.0306 0.0493 0.019 0.026 0.0128 0.0178 0.0105 0.0236 0.0118
Eisosomes 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0003 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0 0.0003 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0062 0.0025 0.0031 0.0034 0.003 0.0024 0.013 0.0019 0.0042 0.0042 0.0049 0.0032 0.008 0.0022 0.0057 0.0028 0.0071 0.0049
Endosome 0.0141 0.0029 0.0032 0.0131 0.0056 0.0073 0.0263 0.0021 0.0187 0.0312 0.0112 0.0131 0.0186 0.0069 0.0081 0.017 0.0107 0.0119
Golgi 0.0061 0.0003 0.0008 0.0026 0.0005 0.0017 0.0158 0.0005 0.0043 0.0259 0.004 0.0066 0.0046 0.0047 0.0049 0.0033 0.0037 0.0035
Lipid Particles 0.0063 0.0001 0.0012 0.0028 0.0003 0.0041 0.0115 0.0002 0.003 0.0131 0.0243 0.0023 0.0096 0.0009 0.0033 0.0055 0.0041 0.0007
Mitochondria 0.002 0.0005 0.0058 0.0014 0.0014 0.0029 0.0063 0.0005 0.012 0.0131 0.0016 0.0035 0.002 0.0005 0.0095 0.0014 0.0084 0.0021
None 0.2469 0.2134 0.3165 0.2437 0.3365 0.2011 0.1557 0.2621 0.1655 0.2094 0.1683 0.1626 0.1878 0.1409 0.2054 0.316 0.2268 0.166
Nuclear Periphery 0.0049 0.0009 0.0012 0.0007 0.0013 0.0025 0.0097 0.0007 0.0026 0.0012 0.001 0.0012 0.0125 0.0006 0.0024 0.0036 0.0066 0.0026
Nucleolus 0.0009 0.0002 0.0003 0.0001 0.0001 0.0007 0.0009 0.0002 0.0002 0.0003 0.0003 0.0002 0.0007 0.0001 0.0002 0.0004 0.0006 0.0007
Nucleus 0.004 0.0022 0.0019 0.0013 0.002 0.0018 0.0093 0.0016 0.0018 0.0013 0.0013 0.0014 0.0036 0.0017 0.0022 0.0026 0.0106 0.003
Peroxisomes 0.0074 0.0002 0.0011 0.0121 0.0014 0.003 0.0107 0.002 0.0059 0.0538 0.0134 0.0051 0.0127 0.0006 0.0091 0.0033 0.0121 0.0018
Punctate Nuclear 0.0037 0.0007 0.0025 0.0007 0.0015 0.0019 0.0096 0.0015 0.0014 0.0013 0.0114 0.0032 0.0104 0.0007 0.002 0.0087 0.0037 0.0034
Vacuole 0.0038 0.0029 0.0019 0.002 0.0027 0.0011 0.0037 0.0017 0.0025 0.0029 0.0026 0.0012 0.0023 0.0014 0.0022 0.0022 0.0024 0.0014
Vacuole Periphery 0.0007 0.0003 0.0003 0.0004 0.0008 0.0003 0.0016 0.0001 0.0008 0.0009 0.0005 0.0003 0.0009 0.0001 0.0005 0.0006 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.175 21.0007 32.1408 65.197 48.5437 60.8994 37.1591 60.4605 103.0956 64.1658
Translational Efficiency 1.7321 1.3143 1.3152 1.7284 1.508 1.8653 1.6803 1.4387 1.5448 1.5594

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Emi2

Emi2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emi2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available