Standard name
Human Ortholog
Description Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.16 0.34 0.23 0.38 0.37 0.41 0.42 0.19 0.22 0.23 0.26 0.13 0.27 0.31 0.21 0.27 0.26 0 0.06 0.1 0.1 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.1 0.1 0.08 0.06 0.06 0 0.06 0.05 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.12 0 0.14 0.09 0.42 0.45 0.3 0.71 0.72 0.74 0.58 0.24 0.07 0.12 0.05 0.05 0.07 0 0 0.06 0.13 0.13 0.1
Nucleus 0.67 0.51 0.49 0.55 0.33 0.2 0.19 0.21 0.22 0.17 0.16 0.24 0.53 0.4 0.28 0.42 0.38 0.41 0.52 0.43 0.32 0.16 0.15 0.09
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.06 0.11 0.05 0.06 0.09 0 0.05 0 0.05 0 0.05 0 0 0 0 0.09 0.11 0.08 0.07 0.1 0.1
Vac/Vac Membrane 0.12 0.27 0.08 0 0.06 0 0 0.06 0 0.05 0 0.05 0.18 0.24 0.27 0.31 0.3 0.31 0.16 0.18 0.26 0.37 0.36 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0 0 1 0 1 4
Bud 2 0 3 0 1 5 2 7 5 11 13 4 0 0 2 0 0 0 0 5 7 2 3 6
Bud Neck 0 0 13 3 15 7 10 10 3 5 3 8 0 0 0 0 1 0 0 1 2 0 4 7
Bud Site 0 1 0 0 4 3 3 5 8 11 12 15 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 0 0 0 1 0 2 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 2 29 86 37 65 94 118 98 70 94 71 81 21 45 50 40 45 41 3 14 44 18 13 10
Endoplasmic Reticulum 0 2 1 0 1 0 5 0 0 0 0 2 0 0 1 12 10 2 0 0 4 2 2 5
Endosome 1 7 1 7 4 8 0 7 1 0 1 4 3 10 14 18 17 12 7 13 13 10 10 21
Golgi 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 3 0 1 0
Mitochondria 26 22 10 22 16 108 128 70 267 301 230 182 38 11 20 9 8 11 1 10 23 22 26 21
Nucleus 69 94 125 88 56 50 54 49 83 70 51 74 84 66 46 80 63 64 68 106 138 28 30 18
Nuclear Periphery 0 1 0 0 0 0 0 0 1 0 1 0 0 0 2 0 0 1 0 3 3 0 2 2
Nucleolus 2 3 0 0 0 0 1 0 1 1 1 0 0 1 1 0 1 1 10 13 9 6 5 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
SpindlePole 2 4 12 10 18 12 18 21 13 22 12 16 3 9 2 3 7 1 12 28 35 12 20 20
Vac/Vac Membrane 12 50 20 4 11 11 5 13 16 20 12 15 29 40 44 59 50 48 21 44 111 65 75 72
Unique Cell Count 103 184 254 159 171 255 285 234 375 420 312 314 160 165 162 189 168 156 133 251 428 180 208 210
Labelled Cell Count 117 214 273 172 191 299 344 281 468 536 407 404 179 182 183 223 203 182 133 251 428 180 208 210


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.2 4.9 4.3 4.9 3.8 3.9 4.4 3.4 3.3 3.1 3.6 4.3 4.4 4.4 7.4 7.5 6.8 3.9 4.2 4.5
Std Deviation (1e-4) 0.6 0.6 1.1 1.0 1.4 1.1 1.1 1.4 0.7 0.8 1.0 0.7 1.1 1.1 1.2 2.1 2.5 1.8 0.8 1.5 1.4
Intensity Change (Log2) – – – -0.18 -0.0 -0.37 -0.33 -0.17 -0.54 -0.57 -0.66 -0.46 -0.18 -0.16 -0.16 0.58 0.62 0.48 -0.32 -0.24 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.3 5.9 6.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1909 -0.3034 -0.2136 0.2387 -1.1699 -0.1249 0.1696 -0.1577 -0.0769 0.3701 0.4164 -0.0508 -0.436 -0.1706 -0.4127 -0.204 -0.6118 -0.4437
Actin 0.0111 0.0001 0.029 0.0478 0.0004 0.0052 0.032 0.0004 0.0085 0.0121 0.0105 0.0034 0.0138 0.0002 0.0002 0.026 0.0006 0.0006
Bud 0.0007 0.0001 0.0003 0.0015 0 0.0003 0.0005 0.0001 0.0003 0.0013 0.0012 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0
Bud Neck 0.0032 0.0006 0.0019 0.0027 0.0016 0.0118 0.0124 0.0005 0.0056 0.0029 0.0026 0.0033 0.0036 0.0006 0.0003 0.001 0.001 0.0025
Bud Periphery 0.0025 0.0001 0.0003 0.0026 0 0.0009 0.0008 0.0001 0.0006 0.002 0.0033 0.0007 0.0002 0.0001 0 0.0001 0.0001 0.0001
Bud Site 0.0018 0.0008 0.0021 0.0029 0.0001 0.0013 0.0024 0.0005 0.0079 0.0374 0.0042 0.0008 0.0012 0.0006 0.0002 0.0002 0.0004 0.0001
Cell Periphery 0.0003 0 0.0001 0.0002 0 0.0001 0.0002 0 0.0002 0.0007 0.0006 0.0001 0.0001 0 0 0 0 0
Cytoplasm 0.0247 0.0275 0.0242 0.005 0.0104 0.0112 0.0308 0.0276 0.0142 0.0117 0.0075 0.0084 0.0117 0.0221 0.014 0.0296 0.0131 0.0035
Cytoplasmic Foci 0.0116 0.0009 0.0075 0.0069 0.002 0.0049 0.0139 0.0006 0.004 0.0083 0.0055 0.0011 0.008 0.0009 0.0014 0.0011 0.0003 0.0014
Eisosomes 0.0001 0 0.0003 0.0003 0 0.0001 0.0002 0 0.0001 0.0003 0.0003 0 0.0002 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0052 0.0025 0.0019 0.0028 0.0013 0.0007 0.0094 0.0021 0.0018 0.0015 0.0031 0.0019 0.0017 0.0015 0.0004 0.002 0.0009 0.0003
Endosome 0.0244 0.0021 0.0101 0.0315 0.0051 0.0028 0.0353 0.0016 0.0099 0.0172 0.0555 0.0098 0.008 0.0017 0.0005 0.0048 0.0019 0.0005
Golgi 0.0038 0 0.0054 0.015 0.0002 0.0011 0.0065 0.0001 0.0038 0.01 0.0269 0.0025 0.0016 0.0001 0 0.0016 0.0001 0.0001
Lipid Particles 0.0112 0.0003 0.0088 0.0294 0.001 0.0025 0.0116 0.0001 0.0107 0.0119 0.0376 0.0022 0.0068 0.0003 0.0001 0.0008 0.0001 0.0001
Mitochondria 0.008 0.0004 0.0017 0.0263 0.0004 0.0025 0.0033 0.0005 0.0019 0.0301 0.0377 0.0017 0.0013 0.0003 0.0004 0.0015 0.0009 0.0002
None 0.0157 0.0455 0.0633 0.0021 0.0069 0.039 0.0235 0.0182 0.0278 0.0029 0.006 0.0009 0.0353 0.0169 0.0465 0.0392 0.0143 0.0081
Nuclear Periphery 0.0536 0.0436 0.0323 0.0077 0.0633 0.0183 0.0611 0.0312 0.0525 0.0127 0.0328 0.0197 0.0288 0.0281 0.0219 0.0762 0.0165 0.0089
Nucleolus 0.0229 0.0213 0.014 0.0197 0.0198 0.0315 0.0141 0.0122 0.0166 0.0083 0.0132 0.027 0.0177 0.0169 0.019 0.0112 0.0199 0.0311
Nucleus 0.6954 0.8046 0.7015 0.7117 0.7628 0.805 0.6676 0.8536 0.7775 0.8035 0.6885 0.8835 0.7916 0.8606 0.8287 0.6596 0.9107 0.8803
Peroxisomes 0.0159 0.0001 0.0057 0.0209 0.0001 0.0012 0.0088 0.0001 0.0023 0.0059 0.0031 0.0006 0.0017 0.0001 0 0.0004 0.0001 0
Punctate Nuclear 0.0748 0.0476 0.0882 0.0551 0.1229 0.0585 0.0596 0.049 0.0501 0.0117 0.0502 0.0299 0.0649 0.0478 0.0655 0.1435 0.0181 0.0618
Vacuole 0.0089 0.0016 0.0009 0.0058 0.0008 0.0006 0.0039 0.0009 0.0027 0.0055 0.0058 0.0012 0.001 0.0009 0.0003 0.0004 0.0005 0.0003
Vacuole Periphery 0.0043 0.0005 0.0005 0.0022 0.0008 0.0004 0.0022 0.0004 0.0009 0.0021 0.0039 0.0007 0.0006 0.0003 0.0002 0.0007 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.8065 7.2309 9.7844 8.9983 9.8705 4.2799 10.4291 9.869 9.5455 8.1202
Translational Efficiency 0.9412 0.713 0.5193 0.6045 0.3523 0.8545 0.5162 0.5413 0.521 0.5324

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
Localization
Cell Percentages nucleus (56%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Urc2

Urc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Urc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available