Standard name
Human Ortholog
Description Putative protein serine/threonine kinase; localizes to the nucleus and cytoplasm; required for efficient spore packaging, prospore membrane development and closure and localization of enzymes involved in spore wall synthesis; interacts with and required for Ssp1p phosphorylation and turnover; member of the GCKIII subfamily of STE20 kinases; multiply phosphorylated on S/T residues; interacts with 14-3-3 proteins, Bmh1p and Bmh2p; expressed at the end of meiosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.96 – 0.97 0.97 0.89 0.89 0.89 0.84 0.69 0.55 0.62 0.99 0.99 0.99 – 0.94 0.93 0.6 0.84 0.54 0.85 0.88 0.79
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0.1 0.07 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.36 0.05 – 0.16 0.05 0.22 0.22 0.2 0.38 0.43 0.6 0.5 0 0 0 – 0.05 0 0.2 0.05 0.21 0.05 0 0.08
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 1 2 0 1 4
Bud 0 0 – 0 0 0 0 2 2 2 1 2 0 0 0 – 0 0 1 1 0 0 0 3
Bud Neck 0 0 – 0 0 1 0 7 0 1 3 0 0 0 0 – 1 2 0 1 0 0 0 2
Bud Site 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 1 – 0 0 0 1 1 1 0 0 0 1 0 1 – 3 5 0 0 0 0 1 0
Cytoplasm 104 172 – 31 37 16 93 171 46 47 49 16 168 242 308 – 197 149 79 148 20 80 197 163
Endoplasmic Reticulum 1 1 – 0 0 0 1 0 0 0 0 0 0 1 0 – 21 12 1 1 0 0 0 1
Endosome 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 – 2 3 2 1 2 0 1 2
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 – 2 1 1 0 0 0 1 0
Mitochondria 43 9 – 5 2 4 23 39 21 29 53 13 1 0 1 – 11 6 26 9 7 4 9 15
Nucleus 0 0 – 0 0 0 0 2 0 0 0 0 0 1 0 – 0 2 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0
Nucleolus 0 0 – 0 0 0 1 0 0 1 0 0 0 0 0 – 1 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 1 5 0 1 4 2 0 0 0 – 0 2 4 2 0 0 1 1
Vac/Vac Membrane 1 1 – 0 0 0 0 0 1 1 0 0 0 0 0 – 7 2 9 3 0 1 2 5
Unique Cell Count 121 179 32 38 18 104 193 55 68 89 26 170 244 310 209 161 132 178 38 95 225 208
Labelled Cell Count 150 184 36 39 21 120 229 71 82 110 33 171 244 311 245 185 132 178 38 95 225 208


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.6 – 3.1 3.9 2.7 2.6 3.3 2.8 2.5 2.5 2.4 4.2 4.0 4.1 4.8 5.4 5.5 3.5 3.9 4.7
Std Deviation (1e-4) 0.6 1.4 – 1.6 2.0 0.9 1.8 2.4 1.6 1.2 1.3 0.9 1.4 1.5 1.4 1.8 2.1 1.9 1.0 1.4 1.3
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.771 0.4456 0.4665 0.0217 0.7138 0.2835 0.7242 0.8446 0.8644 0.704 0.9036 1.0647 1.2095 1.1885 1.2588 0.8249 0.7329 1.1206
Actin 0.0654 0.0013 0.02 0.001 0.0039 0.0027 0.02 0 0.0045 0.0163 0.0022 0.0088 0.0671 0.0001 0.0264 0.0217 0 0.006
Bud 0.0028 0.0042 0.0067 0.0004 0.003 0.0006 0.0005 0.0003 0.0017 0.001 0.0005 0.004 0.0018 0.0002 0.0031 0.0012 0 0.0001
Bud Neck 0.0098 0.0001 0.0007 0.0001 0.0013 0.0011 0.0033 0 0.0003 0.0002 0.0002 0.0003 0.001 0.0001 0.0031 0.0167 0 0.0071
Bud Periphery 0.0095 0.0071 0.0119 0.0009 0.0138 0.0013 0.0014 0.0015 0.0007 0.0027 0.0006 0.0018 0.0052 0.0003 0.0057 0.0039 0 0.0005
Bud Site 0.0189 0.0098 0.006 0.0003 0.0062 0.0005 0.0054 0.0005 0.0021 0.001 0.0007 0.0003 0.0062 0.001 0.0157 0.0214 0 0.0001
Cell Periphery 0.0013 0.0003 0.0004 0.0001 0.0004 0.0001 0.0007 0.0001 0.0003 0.0003 0.0001 0.0001 0.001 0.0001 0.0026 0.0036 0 0.0001
Cytoplasm 0.0318 0.0286 0.041 0.0306 0.0802 0.0794 0.0099 0.0111 0.0233 0.0105 0.0188 0.0251 0.0208 0.0274 0.023 0.0257 0.0333 0.0334
Cytoplasmic Foci 0.0158 0.0042 0.0088 0.0238 0.0358 0.0176 0.0064 0.001 0.0134 0.0211 0.0044 0.0083 0.0119 0.0112 0.0071 0.0031 0.0099 0.0015
Eisosomes 0.0006 0.0001 0.0003 0.0002 0.0002 0.0001 0.0013 0.0002 0.0005 0.0004 0.0001 0.0002 0.0016 0.0002 0.0011 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0032 0.001 0.0029 0.0006 0.0036 0.0007 0.0039 0.0001 0.0006 0.0006 0.0003 0.0003 0.0044 0.0003 0.0027 0.0011 0.0002 0.0002
Endosome 0.0129 0.0007 0.0091 0.0043 0.0499 0.0069 0.0157 0.0001 0.0024 0.006 0.0239 0.0084 0.0127 0.0024 0.0021 0.0035 0.0003 0.0001
Golgi 0.0104 0.0008 0.0059 0.0027 0.0127 0.0062 0.0051 0 0.0008 0.0041 0.0171 0.011 0.0063 0.0002 0.001 0.0012 0 0.0002
Lipid Particles 0.0097 0.002 0.0036 0.0188 0.0172 0.0044 0.0056 0.0001 0.003 0.0058 0.0013 0.0073 0.0093 0.0007 0.0015 0.0016 0.0001 0.0001
Mitochondria 0.0059 0.001 0.0082 0.001 0.0767 0.0043 0.0164 0.0003 0.0006 0.0177 0.0505 0.0132 0.0108 0.0003 0.0016 0.0011 0.0001 0.0002
None 0.7528 0.9272 0.8558 0.9044 0.6523 0.8626 0.8965 0.9841 0.9295 0.9076 0.8753 0.898 0.8004 0.9532 0.8715 0.8832 0.9514 0.9494
Nuclear Periphery 0.0094 0.0004 0.0047 0.0003 0.0072 0.0032 0.0027 0 0.0002 0.0006 0.0001 0.0002 0.008 0.0002 0.0052 0.0005 0.0001 0.0001
Nucleolus 0.0014 0.0004 0.0003 0.0002 0.0005 0.001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0002 0.0013 0.0003 0.002 0.0009 0.0002 0
Nucleus 0.0085 0.0017 0.0067 0.0004 0.0055 0.0009 0.0006 0.0001 0.0004 0.0002 0.0001 0.0009 0.0023 0.0004 0.0048 0.0009 0.0003 0.0001
Peroxisomes 0.0161 0.0069 0.0035 0.0077 0.0103 0.0017 0.0015 0.0001 0.0145 0.0014 0.0011 0.0105 0.0197 0.0005 0.0122 0.0007 0.0001 0.0001
Punctate Nuclear 0.0035 0.0014 0.0013 0.0016 0.0014 0.0031 0.0008 0.0001 0.0005 0.0009 0.0001 0.0006 0.0053 0.0007 0.0067 0.0005 0.0038 0.0002
Vacuole 0.0065 0.0006 0.0013 0.0005 0.0132 0.001 0.001 0.0002 0.0005 0.0009 0.0015 0.0004 0.0018 0.0004 0.0006 0.0067 0.0001 0.0001
Vacuole Periphery 0.0039 0.0001 0.0007 0.0001 0.0049 0.0005 0.0007 0 0.0001 0.0007 0.001 0.0002 0.0013 0 0.0002 0.0005 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 0.3745 3.3482 1.9876 1.1104 1.4568 3.7434 1.3541 0.6855 1.9083 0.9558
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Putative protein serine/threonine kinase; localizes to the nucleus and cytoplasm; required for efficient spore packaging, prospore membrane development and closure and localization of enzymes involved in spore wall synthesis; interacts with and required for Ssp1p phosphorylation and turnover; member of the GCKIII subfamily of STE20 kinases; multiply phosphorylated on S/T residues; interacts with 14-3-3 proteins, Bmh1p and Bmh2p; expressed at the end of meiosis
Localization
Cell Percentages cytoplasm (19%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sps1

Sps1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sps1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available