Standard name
Human Ortholog
Description ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 – 0.97 0.98 0.92 0.91 0.81 0.82 0.7 0.78 0.6 0.71 0.99 0.99 0.99 0.97 0.96 0.95 0.89 0.84 0.79 0.92 0.93 0.9
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 – 0 0.07 0 0.21 0.32 0.28 0.47 0.45 0.56 0.52 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 – 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 4 1 2 0 2 2
Bud 1 – 4 1 1 4 3 3 4 5 1 3 2 0 1 0 0 1 0 0 2 1 2 5
Bud Neck 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 2 0 1 1
Bud Site 0 – 0 0 0 1 0 1 1 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 – 0 0 0 3 2 3 8 6 5 9 1 4 3 6 4 5 2 0 1 0 0 0
Cytoplasm 295 – 102 82 136 198 240 278 201 144 141 199 198 426 371 339 228 173 281 37 88 156 282 280
Endoplasmic Reticulum 6 – 0 0 5 2 0 1 4 0 1 0 0 0 0 13 19 7 1 0 2 1 2 2
Endosome 0 – 0 0 0 2 3 0 0 0 0 1 0 0 2 5 2 1 5 0 0 0 3 1
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 1 0 2 0 2
Mitochondria 21 – 4 6 6 46 93 96 136 83 130 145 1 3 1 10 8 5 6 0 3 3 2 2
Nucleus 0 – 1 0 2 1 1 4 6 3 2 1 1 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 1 – 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 2 0 0 0 1 1
Vac/Vac Membrane 0 – 0 0 2 3 1 6 2 1 1 1 1 0 1 2 2 3 1 0 5 1 1 2
Unique Cell Count 311 105 84 148 217 295 339 289 185 234 280 201 429 375 348 238 182 315 44 112 171 303 312
Labelled Cell Count 327 112 90 153 261 343 392 364 243 282 360 204 435 380 377 264 196 315 44 112 171 303 312


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 – 4.6 4.6 4.6 4.1 3.7 4.1 3.7 3.7 3.6 3.4 5.8 5.6 5.6 5.4 5.9 6.6 5.4 5.6 6.3
Std Deviation (1e-4) 0.5 – 1.0 1.4 1.1 1.3 1.2 1.3 0.9 1.6 1.1 0.7 1.2 1.0 1.4 1.4 1.6 2.3 1.2 1.1 1.4
Intensity Change (Log2) – – – -0.0 -0.0 -0.17 -0.31 -0.2 -0.32 -0.31 -0.36 -0.44 0.31 0.28 0.27 0.21 0.35 0.52 0.23 0.27 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -1.7 -2.0 -3.9 -3.9 -5.7 -4.4 -7.0 -5.5 0.8 1.9 1.4 0.2 -0.6 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9043 2.0912 2.0863 1.4659 2.1074 1.8695 1.2946 1.7823 1.3374 1.1946 0.762 1.2546 2.6965 2.5322 2.2489 2.2908 2.0435 2.3113
Actin 0.0647 0.0011 0.0279 0.0097 0.0488 0.0202 0.0387 0.0001 0.0083 0.0034 0.0001 0.0136 0.0732 0.0004 0.0083 0.0037 0.0792 0.0038
Bud 0.0016 0.0011 0.001 0.0074 0.0011 0.0004 0.0041 0 0.0008 0.0009 0 0.0004 0.0007 0.0001 0.0007 0.0001 0.0008 0.0045
Bud Neck 0.0057 0.0005 0.0044 0.027 0.0051 0.0038 0.0003 0.0001 0.0003 0.0003 0.0001 0.0005 0.0256 0.0001 0.0003 0.0004 0.0026 0.0067
Bud Periphery 0.0027 0.0009 0.0023 0.0177 0.0019 0.0009 0.0096 0 0.0005 0.0006 0 0.0006 0.0011 0.0001 0.0017 0.0001 0.0012 0.0007
Bud Site 0.0123 0.0209 0.0064 0.0305 0.0043 0.0015 0.0019 0.0005 0.0023 0.0005 0 0.0004 0.0086 0.001 0.001 0.0002 0.0014 0.0004
Cell Periphery 0.001 0.0006 0.0007 0.0007 0.0004 0.0005 0.0004 0.0004 0.0004 0.0002 0.0001 0.0001 0.0007 0.0004 0.0006 0.0001 0.0004 0.0001
Cytoplasm 0.4755 0.6841 0.6323 0.4989 0.7221 0.6693 0.2233 0.5726 0.4608 0.4523 0.6937 0.5658 0.4775 0.7626 0.6619 0.7149 0.7241 0.8063
Cytoplasmic Foci 0.0227 0.0056 0.0101 0.0097 0.0055 0.0187 0.0419 0.0067 0.0295 0.0654 0.0027 0.0077 0.0204 0.0236 0.0062 0.0054 0.0038 0.0052
Eisosomes 0.0006 0.0001 0.0002 0.0002 0.0006 0.0001 0.0006 0 0.0004 0.0001 0 0 0.0011 0 0.0014 0.0001 0.0007 0
Endoplasmic Reticulum 0.0104 0.0028 0.0071 0.0014 0.003 0.0054 0.0102 0.0042 0.0034 0.0017 0.002 0.0026 0.0085 0.002 0.0059 0.0024 0.0045 0.002
Endosome 0.0431 0.0017 0.0101 0.0041 0.013 0.0114 0.0477 0.003 0.0199 0.0856 0.0011 0.0052 0.0168 0.0021 0.0058 0.0048 0.0126 0.0021
Golgi 0.0215 0.0002 0.0022 0.0021 0.0084 0.0047 0.0255 0.0002 0.022 0.0211 0.0001 0.0052 0.0102 0.0004 0.005 0.0004 0.0079 0.0006
Lipid Particles 0.0227 0.0002 0.0028 0.0008 0.0059 0.0102 0.011 0.0003 0.0037 0.0153 0 0.0047 0.0143 0.0004 0.002 0.0003 0.0049 0.0007
Mitochondria 0.0123 0.0001 0.0015 0.002 0.011 0.0027 0.0108 0 0.0056 0.0031 0 0.0047 0.0151 0.0001 0.0021 0.0009 0.003 0.0006
None 0.2662 0.2761 0.2582 0.3782 0.1065 0.2298 0.5298 0.4099 0.4067 0.3102 0.2977 0.3778 0.288 0.2039 0.2929 0.2548 0.1401 0.1592
Nuclear Periphery 0.0095 0.0005 0.0083 0.0005 0.0408 0.0081 0.0136 0.0004 0.0049 0.0003 0.0001 0.0003 0.0091 0.0003 0.0009 0.0013 0.0057 0.0007
Nucleolus 0.0017 0.0001 0.0008 0.0016 0.0011 0.0003 0.0006 0 0.0004 0.0001 0 0.0001 0.0005 0 0.0001 0.0005 0.0004 0.0001
Nucleus 0.0029 0.0013 0.0095 0.0018 0.0075 0.0024 0.0128 0.0003 0.0122 0.0004 0.0003 0.0004 0.002 0.0006 0.0008 0.0053 0.0016 0.0024
Peroxisomes 0.0116 0.0001 0.0019 0.0018 0.0029 0.0032 0.0057 0 0.0069 0.0357 0 0.0081 0.0184 0.0006 0.0007 0.0003 0.0009 0.0003
Punctate Nuclear 0.0034 0.0008 0.0067 0.0012 0.004 0.004 0.0038 0.0002 0.0091 0.0006 0.0001 0.0012 0.0051 0.0008 0.0005 0.0032 0.0022 0.003
Vacuole 0.0057 0.0011 0.005 0.0025 0.0021 0.0014 0.0047 0.0008 0.0015 0.0017 0.0017 0.0004 0.0014 0.0004 0.0009 0.0007 0.0009 0.0003
Vacuole Periphery 0.0021 0.0001 0.0007 0.0003 0.004 0.0009 0.0029 0 0.0005 0.0005 0.0001 0.0001 0.0017 0 0.0003 0.0002 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9914 12.9338 14.6488 21.1398 17.2779 5.3275 18.2395 19.5109 14.7918 23.967
Translational Efficiency 0.6139 0.6946 0.5178 0.3145 0.4745 1.5676 0.4993 0.4177 0.5132 0.3823

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
Localization
Cell Percentages cytoplasm (55%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Age1

Age1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Age1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available