Standard name
Human Ortholog
Description Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase; catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.98 0.87 0.86 0.85 0.71 0.73 0.7 0.71 0.6 0.64 0.98 0.97 0.96 0.88 0.89 0.96 0.92 0.93 0.91 0.96 0.94 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.19 0.09 0.25 0.16 0.38 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.18 0.18 0.12 0.17 0.18 0.12 0.18 0.15 0.18 0 0 0.06 0.13 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud 0 0 0 0 0 2 7 4 7 4 12 13 0 0 0 0 0 0 1 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 2 1 9 2 5 6 4 8 4 10 3 1 1 9 11 5 0 0 0 0 0 0
Cytoplasm 192 277 279 327 528 418 401 486 302 346 203 294 320 428 468 257 203 145 183 260 260 271 472 410
Endoplasmic Reticulum 2 3 2 0 2 4 1 4 3 2 0 3 3 4 2 8 7 7 1 1 1 1 6 5
Endosome 0 0 0 1 1 0 0 0 0 1 0 1 0 0 0 1 1 0 0 1 2 0 1 0
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 0 1 0 0
Mitochondria 3 0 0 2 6 23 105 60 106 76 128 98 0 0 0 1 3 1 0 2 6 0 5 0
Nucleus 6 3 2 3 6 14 20 27 12 18 10 18 2 0 3 8 5 1 4 3 4 2 3 1
Nuclear Periphery 0 0 0 0 0 1 3 4 2 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Vac/Vac Membrane 0 0 4 69 112 58 98 117 51 87 52 85 5 14 29 39 21 4 5 3 2 1 2 3
Unique Cell Count 200 279 284 378 616 491 565 665 429 490 338 460 325 441 485 293 227 151 199 280 286 282 504 430
Labelled Cell Count 205 284 290 404 664 522 642 710 487 543 409 524 333 447 503 326 252 164 199 280 286 282 504 430


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 12.6 10.2 9.7 9.5 7.5 6.5 7.2 6.1 6.3 5.6 6.1 10.6 10.7 10.5 14.4 15.2 14.8 9.9 11.1 11.3
Std Deviation (1e-4) 1.3 2.1 1.6 1.6 1.6 1.3 1.4 1.3 1.3 1.1 1.4 1.2 1.6 1.6 1.5 3.0 3.1 3.0 1.7 1.9 2.0
Intensity Change (Log2) – – – -0.08 -0.11 -0.45 -0.65 -0.51 -0.74 -0.7 -0.88 -0.75 0.05 0.06 0.03 0.49 0.57 0.53 -0.05 0.12 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.1 3.0 0
Cytoplasm -5.4 -5.7 -5.8 -9.4 -9.0 -9.4 -9.4 -11.4 -10.8 0.2 -1.0 -1.4 -4.9 -4.3 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 7.8 5.2 9.1 7.0 11.6 8.3 0 0 0 0 0 0
Nucleus 0 0 2.0 2.5 2.8 2.0 2.5 2.0 2.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 6.8 7.0 5.1 6.7 6.8 5.1 6.8 6.1 7.0 0 1.5 3.0 5.4 4.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.3916 10.8574 9.3678 9.1852 7.7167 9.0457 7.3148 11.5817 9.3809 8.1195 7.6584 8.4432 7.2234 9.5511 8.7391 7.1038 7.0592 8.3706
Actin 0.0303 0.0002 0.0282 0.0122 0.03 0.019 0.0147 0.0005 0.0178 0.0208 0.0103 0.0033 0.0133 0.0008 0.0053 0.0026 0.005 0.0027
Bud 0.0006 0.0001 0.0015 0.0002 0.0003 0.0001 0.0004 0.0021 0.0006 0.0006 0.0013 0.0003 0.0005 0.0002 0.0001 0.0001 0.0025 0.0007
Bud Neck 0.0006 0.0002 0.0006 0.0016 0.0016 0.0012 0.0008 0.0004 0.0008 0.0012 0.0023 0.0019 0.0006 0.0002 0.0004 0.0004 0.0012 0.001
Bud Periphery 0.0006 0 0.0016 0.0001 0.0002 0 0.0004 0.0012 0.0003 0.0004 0.001 0.0002 0.0008 0.0001 0.0001 0 0.0014 0.0006
Bud Site 0.0017 0.0002 0.0047 0.0006 0.001 0.0001 0.0013 0.0034 0.0012 0.0037 0.0013 0.0001 0.0023 0.0013 0.0003 0.0001 0.001 0.0002
Cell Periphery 0.0002 0 0.0001 0 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0007 0 0 0 0.0001 0
Cytoplasm 0.772 0.9731 0.872 0.8963 0.8386 0.9008 0.8332 0.9573 0.9086 0.7866 0.7846 0.9048 0.8085 0.9516 0.9286 0.867 0.7586 0.9282
Cytoplasmic Foci 0.0215 0.0011 0.0134 0.0189 0.0187 0.0108 0.0236 0.0025 0.0102 0.0312 0.0213 0.007 0.023 0.0041 0.0116 0.0239 0.0343 0.004
Eisosomes 0.0003 0 0.0001 0.0002 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0 0.001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0078 0.001 0.0014 0.0021 0.0012 0.0021 0.0068 0.0011 0.0018 0.0027 0.0016 0.0028 0.0067 0.0012 0.001 0.0011 0.0025 0.0012
Endosome 0.05 0.0005 0.0105 0.0101 0.0158 0.0105 0.0356 0.0043 0.0067 0.0494 0.0321 0.0145 0.0328 0.0027 0.0027 0.0125 0.0584 0.0032
Golgi 0.0097 0 0.0101 0.0018 0.0345 0.0013 0.0047 0.0006 0.0044 0.0245 0.0332 0.0039 0.0111 0.0006 0.003 0.0059 0.0297 0.0021
Lipid Particles 0.0105 0 0.0058 0.0036 0.0221 0.0004 0.0033 0.0001 0.0007 0.0084 0.0227 0.0009 0.0135 0.0003 0.0041 0.031 0.0068 0.0006
Mitochondria 0.0054 0.0001 0.0184 0.0008 0.0094 0.0005 0.004 0.0009 0.0014 0.01 0.0436 0.0013 0.0228 0.0002 0.0066 0.0024 0.0506 0.0025
None 0.0221 0.0037 0.0022 0.0067 0.0016 0.0018 0.0072 0.0022 0.0021 0.0015 0.0035 0.0019 0.0116 0.0031 0.0014 0.0015 0.0031 0.0021
Nuclear Periphery 0.0086 0.0021 0.0022 0.0024 0.002 0.0031 0.013 0.003 0.0056 0.0043 0.003 0.0044 0.0069 0.002 0.0022 0.0019 0.0032 0.0027
Nucleolus 0.0005 0 0.0001 0.0001 0 0 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0003 0 0 0.0001 0.0003 0.0001
Nucleus 0.0159 0.017 0.0179 0.0255 0.0099 0.045 0.0383 0.0178 0.0319 0.0385 0.0205 0.0392 0.0268 0.0296 0.0208 0.0231 0.0151 0.0453
Peroxisomes 0.0041 0 0.007 0.0125 0.0111 0.0009 0.002 0.0002 0.0033 0.0118 0.012 0.0007 0.0083 0.0007 0.0103 0.0245 0.0123 0.0008
Punctate Nuclear 0.0211 0.0003 0.0011 0.0027 0.0007 0.0012 0.0037 0.0009 0.0012 0.0011 0.0027 0.0086 0.0039 0.001 0.0009 0.001 0.0085 0.0012
Vacuole 0.0135 0.0003 0.0007 0.0012 0.0007 0.0007 0.0045 0.0013 0.001 0.0019 0.0017 0.003 0.0035 0.0004 0.0004 0.0006 0.0032 0.0006
Vacuole Periphery 0.0031 0.0001 0.0003 0.0003 0.0004 0.0002 0.0019 0.0003 0.0003 0.0009 0.0009 0.001 0.0013 0.0001 0.0001 0.0002 0.002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.4755 25.9102 33.8122 48.8921 29.8044 31.965 44.9428 37.4975 38.3511 51.1171
Translational Efficiency 0.7706 1.0339 0.7976 0.5722 0.9373 1.2931 0.7566 0.9307 0.9198 0.767

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase; catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cab1

Cab1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cab1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available