Standard name
Human Ortholog
Description Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Cytoplasm 0.88 0.99 0.99 0.92 0.85 0.85 0.71 – 0.73 0.68 0.67 0.68 0.99 0.99 0.97 0.94 0.94 0.89 0.83 0.89 0.95 0.9 0.89 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0 – 0.07 0.06 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0.09 0.19 0.09 0.3 – 0.2 0.22 0.24 0.18 0 0 0 0.09 0.09 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 1 3 1 0 0 0 0 0 0 1 1 1 0 1 3
Bud 0 3 0 0 0 2 0 – 0 0 1 4 0 0 0 0 0 0 1 3 1 13 7 18
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 3 2 7 1 6 – 7 4 8 9 2 2 8 9 3 0 0 0 0 0 0 0
Cytoplasm 92 232 286 197 205 169 233 – 157 149 157 173 287 325 351 102 44 16 120 212 276 295 272 260
Endoplasmic Reticulum 0 0 0 3 1 0 3 – 8 6 1 13 0 0 1 8 0 0 1 0 0 2 3 4
Endosome 1 0 0 0 1 0 0 – 0 0 1 0 0 0 1 0 0 0 0 5 0 1 1 4
Golgi 0 0 2 0 0 0 0 – 0 2 3 1 0 0 0 0 0 0 5 0 2 1 1 1
Mitochondria 4 0 0 3 3 24 13 – 15 14 20 16 1 0 1 2 0 0 1 2 1 1 2 3
Nucleus 1 1 2 0 1 3 5 – 1 5 5 4 1 3 2 0 0 0 1 5 1 0 0 1
Nuclear Periphery 2 0 0 0 0 1 0 – 0 4 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 1 0 2 2 2 1
Vac/Vac Membrane 6 4 0 19 46 17 99 – 42 48 55 46 1 1 7 10 4 2 4 2 0 5 8 5
Unique Cell Count 104 235 289 214 241 199 327 214 219 233 254 289 327 360 108 47 18 145 239 290 328 306 316
Labelled Cell Count 107 242 293 224 264 217 360 231 233 254 267 292 331 371 131 51 18 145 239 290 328 306 316


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.8 7.9 7.9 9.0 7.4 10.1 – 12.7 12.3 14.3 15.3 10.6 12.7 13.8 8.0 8.3 7.5 8.1 8.2 9.3
Std Deviation (1e-4) 1.7 2.0 1.7 2.0 2.3 2.7 2.9 – 5.2 4.5 6.0 6.1 3.4 5.0 6.1 3.1 2.4 1.7 6.9 6.4 7.0
Intensity Change (Log2) – – – 0.01 0.19 -0.09 0.36 – 0.69 0.65 0.86 0.96 0.43 0.69 0.81 0.03 0.08 -0.07 0.05 0.06 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 2.0 0 0 0 0 0 0
Cytoplasm -3.9 -6.1 -6.1 -9.4 – -8.8 -9.8 -10.0 -9.9 0.4 0.6 -1.4 -2.7 -2.6 -3.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 3.9 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.1 3.4 – 4.6 4.4 5.1 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 5.2 7.8 5.1 10.2 – 7.9 8.4 8.7 7.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5318 10.3283 10.0557 9.1321 8.2614 9.2261 3.3512 4.6689 4.2419 3.8793 4.4546 4.3679 16.9429 22.0674 19.2242 17.2103 14.8533 18.0471
Actin 0.0109 0.0002 0.0098 0.0021 0.0209 0.0027 0.0105 0.0011 0.0207 0.006 0.003 0.0038 0.0013 0.002 0.0035 0.0042 0.0005 0.0029
Bud 0.0008 0.0003 0.0012 0.0002 0.0009 0.0009 0.0009 0.0102 0.0132 0.0041 0.0052 0.0041 0.001 0.0023 0.0033 0.0116 0.0013 0.0007
Bud Neck 0.0007 0.0002 0.0009 0.0006 0.0011 0.0028 0.0069 0.0002 0.0006 0.0005 0.0012 0.003 0.0003 0.0002 0.0004 0.0021 0.0006 0.0012
Bud Periphery 0.0005 0.0001 0.0007 0.0001 0.0005 0.0005 0.0005 0.0022 0.0069 0.0015 0.002 0.002 0.0004 0.0006 0.0015 0.0103 0.0004 0.0004
Bud Site 0.0035 0.0003 0.0017 0.0002 0.0009 0.0004 0.0126 0.0078 0.0082 0.0013 0.0012 0.0004 0.0024 0.0031 0.0019 0.0019 0.0003 0.0002
Cell Periphery 0.0002 0 0.0001 0 0.0002 0.0001 0.0004 0.0004 0.0026 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0 0.0001
Cytoplasm 0.8897 0.987 0.9242 0.8935 0.8949 0.9301 0.7607 0.9315 0.8312 0.8262 0.775 0.8783 0.9544 0.9771 0.9558 0.9292 0.9726 0.9575
Cytoplasmic Foci 0.0143 0.001 0.0103 0.0071 0.0065 0.0117 0.0409 0.0036 0.0091 0.0032 0.0316 0.0291 0.0048 0.0017 0.0023 0.002 0.0022 0.0047
Eisosomes 0.0001 0 0.0002 0 0.0004 0 0 0 0.0002 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0056 0.0006 0.0045 0.0053 0.0036 0.0029 0.0057 0.001 0.002 0.0011 0.0303 0.0017 0.0021 0.0006 0.0012 0.0019 0.0015 0.0022
Endosome 0.0206 0.0003 0.006 0.041 0.0136 0.0093 0.0522 0.0008 0.0176 0.0232 0.0304 0.0136 0.0011 0.0003 0.0015 0.0007 0.0008 0.0016
Golgi 0.0036 0 0.0017 0.0056 0.0103 0.0019 0.0046 0.0001 0.0028 0.0003 0.001 0.0083 0.0001 0 0.0002 0.0001 0 0.0001
Lipid Particles 0.0041 0 0.0025 0.0061 0.0081 0.0017 0.0019 0.0001 0.0004 0 0.0006 0.0011 0.0001 0 0 0 0 0.0001
Mitochondria 0.0104 0.0001 0.0031 0.0031 0.009 0.0028 0.0012 0.0006 0.0028 0.0003 0.0018 0.0009 0.0002 0.0001 0.0011 0.0006 0.0001 0.0002
None 0.007 0.0014 0.0056 0.0016 0.0025 0.0048 0.0566 0.0242 0.0655 0.085 0.0401 0.0393 0.0102 0.0022 0.01 0.0046 0.0015 0.0083
Nuclear Periphery 0.0062 0.0011 0.0071 0.01 0.0099 0.0041 0.02 0.0008 0.0016 0.0012 0.0208 0.0011 0.003 0.0012 0.0019 0.0028 0.0025 0.0025
Nucleolus 0.0002 0 0.0002 0.0001 0.0004 0.0002 0.0005 0.0021 0.0008 0 0.0015 0.0003 0.0001 0 0.0001 0.0001 0 0
Nucleus 0.0126 0.0063 0.0128 0.0161 0.0102 0.0176 0.0083 0.0048 0.0064 0.0021 0.0159 0.0081 0.0148 0.0071 0.0133 0.0246 0.0135 0.0144
Peroxisomes 0.0018 0 0.0034 0.0007 0.0019 0.0007 0.0015 0.0002 0.0003 0.0001 0.0005 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001
Punctate Nuclear 0.0025 0.0003 0.0022 0.0015 0.0019 0.003 0.0044 0.0009 0.0051 0.0007 0.0185 0.0032 0.0016 0.0009 0.0008 0.001 0.0012 0.0019
Vacuole 0.0035 0.0004 0.0015 0.0032 0.0011 0.0017 0.0083 0.0045 0.0016 0.0273 0.0169 0.0007 0.0017 0.0003 0.0007 0.0012 0.0007 0.0008
Vacuole Periphery 0.0014 0.0001 0.0004 0.0016 0.0013 0.0005 0.0014 0.0029 0.0005 0.0156 0.0023 0.0002 0.0003 0 0.0002 0.0003 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4538 16.5778 21.5275 14.3699 20.0366 16.9898 12.2221 4.7138 14.5172 11.5024
Translational Efficiency 1.743 1.6408 1.2755 2.6417 1.5504 1.6187 1.546 4.7408 1.7628 1.828

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsp31

Hsp31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsp31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available