Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.52 0.81 0.82 0.28 0.21 0.45 0.28 0.26 0.27 0.3 0.26 0.28 0.46 0.43 0.34 0.73 0.61 0.59 0.69 0.6 0.71 0.54 0.52 0.49
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.05 0 0 0 0 0 0 0.13 0.08 0.18 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.06 0.09 0.5 0.29 0.37 0.21 0.2 0.25 0.27 0.27 0.26 0.22 0.37 0.17 0.29 0.16 0.16 0.18 0.2 0.17 0.26 0.29 0.27
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.47 0.2 0.34 0.32 0.59 0.31 0.6 0.59 0.46 0.45 0.48 0.5 0.56 0.41 0.6 0.45 0.55 0.59 0.08 0.12 0.07 0.11 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 2 0 0 3 3 2 6 4 1 0 0 0 0 0 0 0 1 0 1 5 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 5 3
Bud Site 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 2 0 1 1 0 0 2 1 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 44 117 185 54 55 120 118 98 106 149 95 109 73 85 85 104 100 83 92 97 184 44 121 138
Endoplasmic Reticulum 0 1 0 0 0 2 0 0 1 0 1 2 0 1 0 6 8 8 1 0 0 0 0 2
Endosome 3 0 0 2 8 1 8 7 2 8 4 4 4 2 7 0 0 1 0 1 1 1 5 9
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 3 2 1 0 0 0 0 1
Mitochondria 4 7 0 2 0 6 10 13 52 39 66 44 1 1 2 0 3 2 0 1 1 0 1 1
Nucleus 3 9 20 98 75 97 88 74 99 133 97 102 35 72 44 42 27 23 24 32 43 21 68 75
Nuclear Periphery 2 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 1 2 1 3 7
Vac/Vac Membrane 40 29 76 63 154 83 251 221 182 222 174 195 89 81 152 64 91 83 10 19 18 8 19 24
Unique Cell Count 85 144 226 195 259 264 421 375 398 491 364 390 160 196 252 143 165 140 134 162 259 83 234 282
Labelled Cell Count 96 166 282 223 295 309 479 420 447 559 445 458 202 242 290 217 232 205 134 162 259 83 234 282


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 5.4 7.2 6.8 8.6 8.2 8.3 8.6 7.8 8.5 7.8 8.5 7.8 8.2 8.4 9.9 11.4 12.4 9.0 8.6 8.8
Std Deviation (1e-4) 1.2 0.7 1.3 1.2 1.2 1.3 1.5 1.6 1.8 2.0 1.8 2.2 1.2 1.2 1.2 1.9 2.0 2.7 3.1 2.5 2.1
Intensity Change (Log2) – – – -0.08 0.26 0.19 0.21 0.27 0.12 0.25 0.13 0.24 0.12 0.19 0.23 0.47 0.66 0.79 0.33 0.26 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -11.2 -13.3 -8.3 -13.1 -13.3 -13.3 -12.8 -13.2 -12.9 -7.4 -8.2 -10.6 -2.1 -4.7 -4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.7 4.4 6.8 5.2 0 0 0 0 0 0
Nucleus 9.4 5.6 7.2 3.9 3.6 4.9 5.5 5.3 5.2 3.6 6.9 2.8 5.1 2.3 2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 5.7 -0.5 6.3 6.0 3.0 2.9 3.4 3.9 4.3 1.6 5.8 2.1 4.2 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7096 2.0284 1.7589 2.1922 1.0638 1.9657 4.0392 4.99 4.5895 4.1148 3.6582 4.5837 1.0288 1.6217 1.2358 0.9174 0.8832 1.2119
Actin 0.0843 0.0005 0.0129 0.0032 0.0333 0.001 0.012 0.0003 0.0044 0.0015 0.0012 0.0004 0.0194 0.0003 0.0109 0.0007 0.006 0.0061
Bud 0.0006 0.0002 0.0005 0.0003 0.0006 0.0003 0.0002 0.0008 0.0002 0.0004 0.0002 0.0004 0.0005 0.0003 0.0003 0.0012 0.0002 0.0002
Bud Neck 0.002 0.0005 0.0009 0.0008 0.001 0.0025 0.0061 0.0005 0.0014 0.001 0.0021 0.0028 0.0029 0.0003 0.0006 0.001 0.0008 0.0105
Bud Periphery 0.0012 0.0001 0.0005 0.0001 0.0011 0.0002 0.0002 0.0004 0.0002 0.0002 0.0001 0.0004 0.0006 0.0002 0.0002 0.0007 0.0005 0.0003
Bud Site 0.0028 0.0008 0.0009 0.0003 0.0007 0.0003 0.0025 0.0026 0.0013 0.0005 0.0002 0.0004 0.0068 0.0006 0.001 0.0019 0.0003 0.0003
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0001 0.0001 0 0 0.0002 0.0001 0 0.0002 0.0001 0
Cytoplasm 0.395 0.5685 0.5061 0.3563 0.36 0.4568 0.558 0.7216 0.5848 0.6428 0.5078 0.5442 0.489 0.77 0.6394 0.6538 0.5559 0.601
Cytoplasmic Foci 0.0158 0.0046 0.0099 0.0148 0.0185 0.0098 0.0152 0.0043 0.0108 0.0057 0.0111 0.0037 0.016 0.0072 0.0111 0.0036 0.0015 0.0051
Eisosomes 0.0008 0 0.0001 0.0006 0.0001 0 0.0001 0 0.0001 0 0 0 0.0002 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0078 0.0081 0.0056 0.0068 0.0036 0.0061 0.0044 0.0037 0.0048 0.0047 0.0046 0.0026 0.0063 0.0055 0.006 0.0079 0.0035 0.0031
Endosome 0.0163 0.0058 0.0088 0.0105 0.0422 0.0112 0.0092 0.0069 0.0073 0.0038 0.0064 0.0048 0.0305 0.0052 0.0064 0.0035 0.0042 0.0055
Golgi 0.0093 0.0002 0.0035 0.0047 0.0455 0.0007 0.0028 0.0001 0.0012 0.0005 0.0002 0.0003 0.0079 0.0001 0.0038 0.0001 0.002 0.0018
Lipid Particles 0.0185 0.0004 0.0074 0.0216 0.0189 0.0002 0.0081 0.0001 0.002 0.0001 0.0002 0.0001 0.0134 0.0002 0.0033 0.0001 0.0037 0.0013
Mitochondria 0.0181 0.0004 0.0074 0.0017 0.0093 0.0025 0.0007 0.0003 0.0007 0.0007 0.0005 0.0012 0.0027 0.0002 0.0013 0.0006 0.0577 0.0105
None 0.0336 0.1051 0.0496 0.0732 0.1778 0.0431 0.1178 0.0919 0.165 0.0783 0.2103 0.0902 0.102 0.081 0.0453 0.0446 0.0054 0.0207
Nuclear Periphery 0.0211 0.0189 0.0244 0.0277 0.0091 0.0189 0.0142 0.0056 0.0093 0.007 0.0092 0.0079 0.0162 0.0089 0.0116 0.0128 0.0118 0.0094
Nucleolus 0.0006 0.0004 0.0008 0.0018 0.0003 0.0006 0.0006 0.0003 0.0005 0.0004 0.0006 0.0012 0.0006 0.0001 0.0003 0.001 0.0003 0.0004
Nucleus 0.3357 0.2773 0.3383 0.4252 0.2528 0.4287 0.2208 0.1398 0.1937 0.2465 0.2241 0.3253 0.2614 0.1152 0.2463 0.2544 0.3378 0.316
Peroxisomes 0.011 0.0001 0.0084 0.0342 0.0191 0.0004 0.0064 0.0001 0.0026 0.0003 0.0003 0.0001 0.0078 0.0002 0.0054 0.0001 0.0006 0.0005
Punctate Nuclear 0.022 0.0044 0.0112 0.0113 0.0037 0.0133 0.0183 0.0167 0.0076 0.004 0.0194 0.011 0.013 0.0022 0.0053 0.0086 0.0039 0.0057
Vacuole 0.0022 0.0031 0.0022 0.0038 0.0014 0.0025 0.0016 0.0033 0.0017 0.0013 0.0012 0.0022 0.0018 0.0017 0.0013 0.0028 0.0012 0.0013
Vacuole Periphery 0.001 0.0004 0.0007 0.001 0.001 0.0007 0.0003 0.0005 0.0003 0.0002 0.0003 0.0006 0.0008 0.0003 0.0003 0.0004 0.0021 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.1986 10.5093 14.4827 17.3517 14.1537 13.4282 11.385 15.8805 17.3811 13.6611
Translational Efficiency 4.2674 2.0523 1.6228 1.8788 1.2351 2.022 2.6105 2.0424 2.2352 1.9477

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
Localization
Cell Percentages cytoplasm (59%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ubc8

Ubc8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubc8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available