Standard name | |
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Human Ortholog | |
Description | Peroxisomal matrix protein; well-conserved in fungi; contains tripartite homology domain of thiamine pyrophosphate (TPP) enzymes; targeted to peroxisomes by Pex5p; contains low sequence identity with Pdc1p; mRNA identified as translated by ribosome profiling data |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 1 | 1 | 1 | 0 | 2 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 4 | 10 | 12 | 8 | 10 | – | 7 | 3 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 10 | 3 | 3 | 5 | 1 | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 7 | 5 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 235 | 204 | 192 | 248 | 356 | 397 | 452 | – | 223 | 221 | 207 | 295 | 45 | 50 | 101 | 150 | 201 | 229 | 228 | 186 | 147 | 12 | 39 | 95 |
Endoplasmic Reticulum | 0 | 1 | 2 | 1 | 0 | 2 | 2 | – | 4 | 1 | 0 | 3 | 0 | 0 | 2 | 7 | 11 | 11 | 1 | 0 | 1 | 0 | 0 | 0 |
Endosome | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 14 | 1 | 0 | 1 | 0 | 5 | 10 | – | 25 | 19 | 97 | 45 | 1 | 0 | 1 | 0 | 1 | 0 | 21 | 9 | 22 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 2 | 1 | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 4 | – | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 1 | 1 | 1 | 5 | – | 4 | 6 | 2 | 3 | 0 | 0 | 0 | 15 | 11 | 13 | 6 | 6 | 4 | 0 | 0 | 0 |
Unique Cell Count | 246 | 208 | 194 | 253 | 359 | 407 | 465 | 249 | 242 | 274 | 341 | 45 | 50 | 103 | 168 | 215 | 245 | 275 | 214 | 186 | 14 | 43 | 105 | |
Labelled Cell Count | 255 | 210 | 209 | 266 | 374 | 422 | 488 | 272 | 253 | 319 | 363 | 46 | 50 | 104 | 182 | 233 | 270 | 275 | 214 | 186 | 14 | 43 | 105 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.7 | 4.9 | 4.5 | 5.0 | 4.2 | 4.3 | – | 3.7 | 4.0 | 3.4 | 3.9 | 4.9 | 4.9 | 5.0 | 11.6 | 11.0 | 10.5 | – | 4.5 | 4.9 |
Std Deviation (1e-4) | 0.4 | 1.0 | 0.7 | 1.2 | 1.6 | 0.7 | 1.0 | – | 0.9 | 1.0 | 1.0 | 1.2 | 1.0 | 0.9 | 1.0 | 2.1 | 1.6 | 1.8 | – | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.03 | -0.23 | -0.19 | – | -0.4 | -0.29 | -0.55 | -0.34 | -0.0 | -0.01 | 0.03 | 1.24 | 1.17 | 1.1 | – | -0.13 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.3 | 3.2 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1682 | 0.0215 | 0.0921 | -0.1689 | -0.1104 | -0.1764 | -0.9697 | -0.6696 | -0.707 | -0.9869 | -0.7052 | -0.5476 | -1.0483 | -1.1673 | -1.1262 | -1.3508 | -0.9021 | -1.553 |
Actin | 0.0151 | 0.0003 | 0.0188 | 0.0078 | 0.0026 | 0.0008 | 0.0172 | 0.0001 | 0.0161 | 0.0045 | 0.0254 | 0 | 0.0354 | 0.0008 | 0.0197 | 0 | 0.0894 | 0.0001 |
Bud | 0.0011 | 0.0021 | 0.001 | 0.0018 | 0.0008 | 0.0007 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0008 | 0 | 0.0004 | 0.0013 | 0.0007 | 0 | 0.0555 | 0 |
Bud Neck | 0.0071 | 0.0001 | 0.0031 | 0.0178 | 0.002 | 0.0007 | 0.0006 | 0 | 0.0003 | 0.0014 | 0.0019 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 |
Bud Periphery | 0.0028 | 0.0026 | 0.0019 | 0.0135 | 0.0035 | 0.0025 | 0.0009 | 0 | 0.0002 | 0.0007 | 0.0012 | 0 | 0.0006 | 0.0015 | 0.0007 | 0 | 0.0161 | 0 |
Bud Site | 0.0086 | 0.0055 | 0.0092 | 0.0083 | 0.002 | 0.0005 | 0.0027 | 0.0002 | 0.0003 | 0.0036 | 0.017 | 0 | 0.0012 | 0.0035 | 0.0065 | 0 | 0.0012 | 0 |
Cell Periphery | 0.0003 | 0.0003 | 0.0003 | 0.0011 | 0.0005 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 |
Cytoplasm | 0.0555 | 0.0921 | 0.07 | 0.0833 | 0.0913 | 0.0965 | 0.0429 | 0.0909 | 0.0848 | 0.0232 | 0.0693 | 0.1371 | 0.0835 | 0.1631 | 0.1487 | 0.3338 | 0.1445 | 0.2136 |
Cytoplasmic Foci | 0.0124 | 0.0041 | 0.0138 | 0.0105 | 0.0116 | 0.0084 | 0.0059 | 0.0033 | 0.005 | 0.0299 | 0.0389 | 0.0048 | 0.0298 | 0.0028 | 0.0183 | 0.0057 | 0.0189 | 0.0108 |
Eisosomes | 0.0003 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0034 | 0.0006 | 0.0016 | 0.0011 | 0.0033 | 0.0004 | 0.0016 | 0.0012 | 0.0011 | 0.001 | 0.0013 | 0.0004 | 0.0039 | 0.0007 | 0.0011 | 0.0002 | 0.0019 | 0.0009 |
Endosome | 0.0117 | 0.0009 | 0.0116 | 0.006 | 0.0216 | 0.0016 | 0.0097 | 0.0008 | 0.0029 | 0.0225 | 0.0187 | 0.0008 | 0.0336 | 0.001 | 0.0111 | 0.0005 | 0.0824 | 0.0011 |
Golgi | 0.0047 | 0.0001 | 0.0021 | 0.0023 | 0.0026 | 0.0003 | 0.0026 | 0.0001 | 0.0011 | 0.0051 | 0.0091 | 0 | 0.0199 | 0.0004 | 0.006 | 0 | 0.0427 | 0 |
Lipid Particles | 0.0111 | 0.0001 | 0.0029 | 0.0125 | 0.0148 | 0.0003 | 0.0091 | 0.0003 | 0.0014 | 0.049 | 0.029 | 0.0001 | 0.0149 | 0.0026 | 0.0052 | 0 | 0.0298 | 0.0001 |
Mitochondria | 0.0048 | 0.0003 | 0.0046 | 0.0032 | 0.0099 | 0.0003 | 0.0073 | 0.0002 | 0.0021 | 0.0061 | 0.0049 | 0.0001 | 0.0209 | 0.0025 | 0.0035 | 0.0001 | 0.0181 | 0.0001 |
None | 0.8311 | 0.8858 | 0.8309 | 0.7833 | 0.76 | 0.8795 | 0.8784 | 0.901 | 0.8705 | 0.8318 | 0.7247 | 0.855 | 0.6874 | 0.8147 | 0.7471 | 0.6587 | 0.4624 | 0.7717 |
Nuclear Periphery | 0.0025 | 0.0006 | 0.0044 | 0.0011 | 0.0093 | 0.0008 | 0.0034 | 0.0003 | 0.001 | 0.001 | 0.0028 | 0.0001 | 0.0075 | 0.0003 | 0.0005 | 0.0001 | 0.0035 | 0.0002 |
Nucleolus | 0.0026 | 0.0004 | 0.0011 | 0.005 | 0.011 | 0.0003 | 0.0009 | 0.0001 | 0.0085 | 0.0015 | 0.0017 | 0 | 0.0005 | 0.0009 | 0.0001 | 0 | 0.0006 | 0 |
Nucleus | 0.0038 | 0.0018 | 0.0024 | 0.003 | 0.0115 | 0.0017 | 0.0016 | 0.0005 | 0.0013 | 0.0008 | 0.0017 | 0.0003 | 0.0097 | 0.0007 | 0.0005 | 0.0004 | 0.0099 | 0.0002 |
Peroxisomes | 0.014 | 0.0001 | 0.0125 | 0.0265 | 0.0239 | 0.0006 | 0.0047 | 0.0001 | 0.0007 | 0.0127 | 0.0418 | 0 | 0.0199 | 0.0014 | 0.0277 | 0 | 0.0136 | 0 |
Punctate Nuclear | 0.0039 | 0.0013 | 0.0054 | 0.0089 | 0.0093 | 0.0035 | 0.0056 | 0.0004 | 0.0014 | 0.0021 | 0.0073 | 0.0007 | 0.0234 | 0.0005 | 0.001 | 0.0001 | 0.0029 | 0.0009 |
Vacuole | 0.0023 | 0.0006 | 0.0013 | 0.0021 | 0.0066 | 0.0003 | 0.0026 | 0.0004 | 0.0004 | 0.0018 | 0.0015 | 0.0003 | 0.0031 | 0.0007 | 0.0008 | 0.0002 | 0.0037 | 0.0002 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0006 | 0.0004 | 0.0015 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0005 | 0.0007 | 0 | 0.0033 | 0.0001 | 0.0002 | 0 | 0.002 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.8508 | 13.4799 | 7.3933 | 8.9236 | 8.9254 | 6.5525 | 10.5184 | 8.999 | 9.8124 | 9.6899 |
Translational Efficiency | 0.3225 | 0.3402 | 0.4906 | 0.6338 | 0.3901 | 0.8336 | 0.5791 | 0.8846 | 0.6943 | 0.4885 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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