ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 |
Bud | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 |
Bud Neck | 1 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 0 | 0 | 5 | 0 | 1 | – | 0 | 0 |
Bud Site | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 214 | 87 | – | – | – | – | – | – | – | – | – | – | – | – | – | 95 | 118 | 75 | 158 | 87 | 113 | – | 0 | 0 |
Endoplasmic Reticulum | 1 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 8 | 12 | 6 | 9 | 0 | 4 | – | 0 | 0 |
Endosome | 60 | 2 | – | – | – | – | – | – | – | – | – | – | – | – | – | 10 | 24 | 18 | 59 | 3 | 10 | – | 0 | 0 |
Golgi | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 0 | 2 | 1 | 0 | – | 0 | 0 |
Mitochondria | 12 | 31 | – | – | – | – | – | – | – | – | – | – | – | – | – | 5 | 3 | 2 | 49 | 12 | 45 | – | 0 | 0 |
Nucleus | 20 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 5 | 3 | 1 | 31 | 3 | 6 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 5 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 13 | 1 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 |
SpindlePole | 3 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 1 | 2 | 66 | 6 | 21 | – | 0 | 0 |
Vac/Vac Membrane | 104 | 8 | – | – | – | – | – | – | – | – | – | – | – | – | – | 31 | 39 | 31 | 97 | 12 | 47 | – | 0 | 0 |
Unique Cell Count | 364 | 119 | 139 | 172 | 114 | 527 | 136 | 269 | 2 | 2 | ||||||||||||||
Labelled Cell Count | 415 | 130 | 156 | 202 | 135 | 527 | 136 | 269 | 2 | 2 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 3.5 | – | – | – | – | – | – | – | – | – | – | – | – | – | 5.3 | 5.9 | 6.1 | – | – | – |
Std Deviation (1e-4) | 0.8 | 1.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1.4 | 2.0 | 1.7 | – | – | – |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5566 | 0.3072 | 0.0572 | 0.096 | -0.3867 | 0.3309 | 0.9887 | 1.093 | 1.0049 | 0.7948 | 0.8914 | 0.8986 | 0.8728 | 0.9269 | 0.6492 | 0.419 | 0.6388 | 0.6343 |
Actin | 0.0011 | 0.0002 | 0.0005 | 0.0009 | 0.0006 | 0.0004 | 0.0389 | 0.0001 | 0.0053 | 0.0032 | 0.0116 | 0.0023 | 0.0375 | 0.0012 | 0.0004 | 0.0005 | 0.0013 | 0.0044 |
Bud | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.001 | 0.0006 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0072 | 0.0004 |
Bud Neck | 0.0102 | 0.0028 | 0.0055 | 0.0041 | 0.0203 | 0.0266 | 0.0039 | 0.0022 | 0.0044 | 0.0133 | 0.004 | 0.0147 | 0.002 | 0.0013 | 0.0017 | 0.0051 | 0.0063 | 0.0106 |
Bud Periphery | 0.0005 | 0.0003 | 0.0004 | 0.0003 | 0.0008 | 0.0004 | 0.001 | 0.0002 | 0.0006 | 0.0013 | 0.0009 | 0.0007 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.0036 | 0.0007 |
Bud Site | 0.0157 | 0.0152 | 0.0099 | 0.0035 | 0.0018 | 0.0027 | 0.0079 | 0.0041 | 0.0071 | 0.0286 | 0.0017 | 0.0031 | 0.0029 | 0.0282 | 0.0023 | 0.0049 | 0.0027 | 0.0017 |
Cell Periphery | 0.0006 | 0.0009 | 0.001 | 0.0005 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0003 | 0.0016 | 0.0007 | 0.0003 |
Cytoplasm | 0.2289 | 0.2762 | 0.2016 | 0.2002 | 0.2074 | 0.3039 | 0.1338 | 0.1717 | 0.1377 | 0.1471 | 0.1795 | 0.1941 | 0.0998 | 0.1165 | 0.1137 | 0.1154 | 0.1804 | 0.1125 |
Cytoplasmic Foci | 0.0341 | 0.0266 | 0.028 | 0.0285 | 0.0111 | 0.029 | 0.0391 | 0.0304 | 0.0417 | 0.0608 | 0.0257 | 0.0418 | 0.0952 | 0.0162 | 0.0463 | 0.0145 | 0.0357 | 0.0394 |
Eisosomes | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0206 | 0.016 | 0.0168 | 0.0112 | 0.0142 | 0.0128 | 0.0043 | 0.0046 | 0.0061 | 0.0042 | 0.0047 | 0.0056 | 0.0084 | 0.0062 | 0.0065 | 0.0068 | 0.0114 | 0.0052 |
Endosome | 0.0321 | 0.0303 | 0.0391 | 0.0442 | 0.0155 | 0.0185 | 0.0264 | 0.0166 | 0.0485 | 0.0406 | 0.0311 | 0.0349 | 0.0478 | 0.0339 | 0.0446 | 0.0253 | 0.0602 | 0.0255 |
Golgi | 0.0008 | 0.0008 | 0.001 | 0.0009 | 0.0005 | 0.0004 | 0.0074 | 0.0004 | 0.0058 | 0.019 | 0.0156 | 0.0024 | 0.0138 | 0.0061 | 0.0018 | 0.0008 | 0.0169 | 0.0025 |
Lipid Particles | 0.0077 | 0.0092 | 0.0111 | 0.009 | 0.0024 | 0.0045 | 0.0346 | 0.0063 | 0.0203 | 0.0223 | 0.0048 | 0.0122 | 0.0308 | 0.0056 | 0.0275 | 0.0112 | 0.0286 | 0.0112 |
Mitochondria | 0.001 | 0.0008 | 0.0013 | 0.0011 | 0.0424 | 0.0016 | 0.0044 | 0.0007 | 0.0136 | 0.0057 | 0.0058 | 0.0077 | 0.0064 | 0.0079 | 0.0113 | 0.0012 | 0.0132 | 0.004 |
None | 0.4235 | 0.2445 | 0.352 | 0.3579 | 0.2692 | 0.2117 | 0.4937 | 0.5466 | 0.4231 | 0.212 | 0.4305 | 0.3238 | 0.4583 | 0.5557 | 0.5096 | 0.5556 | 0.421 | 0.4472 |
Nuclear Periphery | 0.0158 | 0.0247 | 0.0194 | 0.0242 | 0.0171 | 0.0109 | 0.0138 | 0.0067 | 0.0199 | 0.0186 | 0.0203 | 0.0165 | 0.0103 | 0.0156 | 0.0162 | 0.0119 | 0.0271 | 0.0152 |
Nucleolus | 0.0233 | 0.0138 | 0.0528 | 0.0358 | 0.0266 | 0.0188 | 0.013 | 0.0176 | 0.0437 | 0.078 | 0.0127 | 0.0297 | 0.0074 | 0.0258 | 0.022 | 0.0624 | 0.0311 | 0.0243 |
Nucleus | 0.1176 | 0.2758 | 0.1841 | 0.1969 | 0.3218 | 0.309 | 0.0591 | 0.1294 | 0.1291 | 0.1774 | 0.1575 | 0.1966 | 0.0823 | 0.1377 | 0.1156 | 0.0854 | 0.1014 | 0.1794 |
Peroxisomes | 0.0009 | 0.0004 | 0.0006 | 0.0008 | 0.0008 | 0.0005 | 0.0267 | 0.0004 | 0.0068 | 0.0137 | 0.0016 | 0.0038 | 0.0358 | 0.0009 | 0.0184 | 0.0014 | 0.005 | 0.002 |
Punctate Nuclear | 0.0482 | 0.0376 | 0.0422 | 0.0499 | 0.0355 | 0.034 | 0.0823 | 0.0471 | 0.0697 | 0.1417 | 0.0859 | 0.0957 | 0.0464 | 0.0209 | 0.0432 | 0.0598 | 0.0325 | 0.1032 |
Vacuole | 0.0158 | 0.0224 | 0.0305 | 0.028 | 0.01 | 0.0123 | 0.0072 | 0.0134 | 0.0139 | 0.0091 | 0.0036 | 0.0124 | 0.0122 | 0.0175 | 0.0154 | 0.0343 | 0.0107 | 0.0089 |
Vacuole Periphery | 0.0008 | 0.0011 | 0.0015 | 0.0016 | 0.0013 | 0.0008 | 0.0012 | 0.0007 | 0.0018 | 0.0016 | 0.0016 | 0.0013 | 0.0011 | 0.0013 | 0.0022 | 0.001 | 0.0027 | 0.0009 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.8881 | 9.402 | 9.2338 | 15.2435 | 10.0267 | 10.2025 | 13.4906 | 12.9266 | 12.8549 | 15.2947 |
Translational Efficiency | 0.2939 | 0.2253 | 0.3141 | 0.2349 | 0.2262 | 0.2476 | 0.2614 | 0.2645 | 0.2436 | 0.1701 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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