Standard name | |
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Human Ortholog | |
Description | Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase; dicyclohexylcarbodiimide binding subunit; required for vacuolar acidification and important for copper and iron metal ion homeostasis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 14 | 20 | 27 | – | 0 | 2 | 0 | – | – |
Bud | – | 0 | 0 | 0 | 1 | 4 | 12 | 11 | 7 | 2 | 13 | 14 | – | – | – | 4 | 7 | 2 | – | 1 | 0 | 0 | – | – |
Bud Neck | – | 0 | 0 | 0 | 1 | 8 | 8 | 7 | 7 | 3 | 6 | 2 | – | – | – | 0 | 0 | 0 | – | 0 | 1 | 0 | – | – |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 4 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 3 | 2 | 1 | 2 | 0 | 3 | 1 | 0 | 1 | 2 | 1 | – | – | – | 29 | 30 | 30 | – | 0 | 1 | 0 | – | – |
Cytoplasm | – | 51 | 166 | 219 | 338 | 287 | 442 | 412 | 203 | 321 | 291 | 415 | – | – | – | 139 | 120 | 107 | – | 3 | 143 | 0 | – | – |
Endoplasmic Reticulum | – | 139 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | 7 | 4 | 1 | – | 164 | 0 | 0 | – | – |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 1 | 0 | – | 1 | 1 | 0 | – | – |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 9 | 12 | 13 | – | 0 | 0 | 0 | – | – |
Mitochondria | – | 0 | 8 | 11 | 44 | 75 | 225 | 159 | 167 | 177 | 204 | 309 | – | – | – | 2 | 2 | 0 | – | 0 | 15 | 0 | – | – |
Nucleus | – | 1 | 0 | 1 | 1 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | 1 | 0 | 0 | – | – |
Nuclear Periphery | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | – | 0 | 0 | 0 | – | – |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – |
SpindlePole | – | 0 | 0 | 1 | 2 | 5 | 12 | 7 | 17 | 8 | 10 | 21 | – | – | – | 1 | 0 | 0 | – | 0 | 1 | 0 | – | – |
Vac/Vac Membrane | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | – | – | – | 17 | 19 | 5 | – | 3 | 1 | 0 | – | – |
Unique Cell Count | 175 | 171 | 223 | 356 | 326 | 566 | 511 | 319 | 429 | 410 | 603 | 204 | 190 | 167 | 179 | 170 | 1 | |||||||
Labelled Cell Count | 196 | 176 | 233 | 390 | 381 | 705 | 604 | 405 | 516 | 532 | 766 | 226 | 215 | 185 | 179 | 170 | 1 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 26.0 | 3.0 | 3.1 | 3.0 | 2.7 | 2.3 | 2.6 | 2.4 | 2.3 | 2.3 | 2.4 | – | – | – | 20.7 | 23.4 | 26.2 | – | – | – |
Std Deviation (1e-4) | – | 6.3 | 0.4 | 1.5 | 1.0 | 1.0 | 0.4 | 0.8 | 0.4 | 0.4 | 0.3 | 0.9 | – | – | – | 5.4 | 6.3 | 6.4 | – | – | – |
Intensity Change (Log2) | – | – | – | 0.05 | -0.01 | -0.13 | -0.4 | -0.19 | -0.34 | -0.37 | -0.39 | -0.32 | – | – | – | 2.79 | 2.97 | 3.13 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3.5 | 4.4 | 5.5 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 4.6 | 4.9 | 5.3 |
Cytoplasm | 0.7 | -1.1 | -3.4 | -5.7 | -5.2 | -8.2 | -6.3 | -7.0 | -7.5 | – | – | – | -7.2 | -7.9 | -7.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 3.3 | 3.7 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3.9 | 4.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 871.5296 | 927.1528 | 1148.6661 | 899.2611 | 922.6411 | 723.7796 | 866.722 | 859.1769 | 1011.9065 | 803.421 |
Translational Efficiency | 1.5457 | 1.4656 | 1.3282 | 1.7285 | 1.5496 | 2.0603 | 1.9364 | 1.7745 | 1.5933 | 1.7428 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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