Standard name
Human Ortholog
Description Protein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.94 0.88 0.76 0.8 0.77 0.72 0.68 0.65 0.61 0.99 0.99 0.99 0.64 0.67 0.69 0.92 0.93 0.94 0.93 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0 0 0.15 0.13 0.17 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0.11 0.14 0.21 0.19 0.19 0.2 0.24 0.26 0 0 0 0.26 0.29 0.22 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 1 0 3 0 0 0 0 6 4 8 0 4 4 2 3 5
Bud 0 0 0 0 0 7 1 2 4 3 8 5 0 0 0 0 0 0 0 0 4 2 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 10 4 11 3 9 18 13 12 21 20 0 2 3 9 8 6 0 0 0 0 0 0
Cytoplasm 293 665 700 420 553 421 598 731 604 511 562 548 493 587 619 275 215 159 284 648 682 372 539 567
Endoplasmic Reticulum 2 3 2 4 4 3 2 7 3 10 8 5 2 3 0 15 11 10 1 2 4 2 8 7
Endosome 1 0 0 1 0 0 1 0 2 2 3 2 1 1 0 37 24 24 5 10 7 2 5 14
Golgi 1 3 3 1 1 0 1 1 0 0 0 0 0 0 1 16 24 8 4 4 2 3 2 3
Mitochondria 2 5 0 3 6 92 9 23 128 97 142 140 0 0 0 17 9 2 0 6 3 2 5 4
Nucleus 4 2 4 3 12 8 26 44 12 24 21 35 1 3 1 8 6 6 0 2 0 1 1 3
Nuclear Periphery 1 0 0 0 0 1 0 0 3 2 0 2 0 0 0 0 0 0 2 0 1 0 0 1
Nucleolus 1 1 0 0 0 1 0 2 0 2 2 1 0 0 1 6 8 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 0 1 0 0 1 1 0 0 1 0 0 0 16 14 9 2 2 5 0 1 6
Vac/Vac Membrane 6 4 23 26 70 77 160 182 156 151 204 231 2 5 2 112 94 52 2 4 1 2 6 8
Unique Cell Count 307 678 721 449 627 557 749 949 839 748 860 903 497 595 624 428 322 232 308 694 728 398 586 638
Labelled Cell Count 313 686 744 462 658 613 807 1011 927 814 974 991 499 601 627 517 417 287 308 694 728 398 586 638


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 7.1 8.9 8.4 8.9 6.3 7.9 8.3 7.3 7.4 7.4 7.3 9.0 9.5 9.8 15.1 16.4 14.0 7.6 8.4 8.3
Std Deviation (1e-4) 1.3 1.0 1.2 1.5 1.3 1.8 1.5 1.5 1.4 1.4 1.4 1.4 1.5 1.7 1.6 4.4 4.4 4.1 1.4 1.6 1.5
Intensity Change (Log2) – – – -0.08 0.01 -0.5 -0.16 -0.1 -0.28 -0.25 -0.25 -0.28 0.02 0.1 0.14 0.77 0.89 0.66 -0.22 -0.09 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.8 0.5 -1.5 -0.3 0.8 0.3 0.3 1.5 1.2 0 -2.0 -1.7 0.9 1.3 0
Cytoplasm -2.9 -6.4 -11.6 -10.3 -11.6 -13.3 -14.5 -15.7 -17.3 2.5 1.9 2.8 -15.0 -13.8 -12.7
Endoplasmic Reticulum 0 0 0 0 0 0 2.3 0 0 0 0 0 4.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 8.0 7.4 8.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 4.3 6.6 0
Mitochondria 0 0 11.3 0 4.2 10.9 10.0 11.4 11.1 0 0 0 5.4 0 0
Nucleus 0 2.3 1.6 4.0 4.9 1.7 3.7 3.0 4.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4.9 0 0
Vacuole 2.2 5.8 7.0 10.5 9.9 9.5 10.1 11.6 12.3 -3.4 -2.9 -3.9 11.7 12.3 9.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6601 3.9163 3.0161 2.9141 2.616 3.1746 6.5856 7.7912 7.1366 6.5242 6.5589 7.0737 6.9399 8.4541 7.5787 7.0941 7.4365 7.8864
Actin 0.0034 0.0012 0.0014 0.0002 0.0001 0.0011 0.0174 0.0034 0.0052 0.025 0.0098 0.0094 0.0008 0.0003 0.0004 0.0002 0.0003 0.0005
Bud 0.0018 0.0036 0.0002 0.0001 0.0001 0.0002 0.0164 0.0271 0.0156 0.0038 0.0189 0.0105 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0003 0.001 0.0003 0.0006 0.0005 0.0011 0.0037 0.0008 0.0066 0.0022 0.0016 0.0093 0.0002 0.0001 0.0002 0.0003 0.0004 0.0007
Bud Periphery 0.0006 0.0009 0.0001 0 0 0.0001 0.0084 0.006 0.0062 0.0022 0.0055 0.007 0 0 0 0 0 0
Bud Site 0.0027 0.0106 0.0004 0.0001 0.0001 0.0005 0.0165 0.0321 0.0209 0.0101 0.0068 0.0037 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0005 0.0003 0.0004 0.0001 0.0004 0.0001 0 0 0 0 0 0
Cytoplasm 0.8892 0.9205 0.8887 0.889 0.9277 0.9139 0.7908 0.8645 0.8131 0.7774 0.7829 0.8159 0.8863 0.9431 0.9351 0.9018 0.9649 0.9333
Cytoplasmic Foci 0.009 0.0121 0.0067 0.0078 0.0076 0.0104 0.0208 0.0087 0.0135 0.0243 0.024 0.0247 0.0038 0.002 0.0036 0.0018 0.0032 0.0045
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0.0002 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0038 0.0021 0.0027 0.0026 0.0017 0.0032 0.0048 0.0018 0.0057 0.0034 0.0031 0.002 0.0034 0.0019 0.0023 0.0022 0.0017 0.0013
Endosome 0.0067 0.0047 0.0042 0.0069 0.0106 0.0067 0.0172 0.0026 0.0154 0.0235 0.017 0.0087 0.0018 0.0007 0.0017 0.0008 0.0015 0.0016
Golgi 0.0005 0.0005 0.0002 0.0001 0.0002 0.0001 0.0041 0.0003 0.0034 0.0096 0.0049 0.0023 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Lipid Particles 0.0002 0.0002 0.0001 0 0 0.0001 0.0034 0.0001 0.0034 0.0037 0.0188 0.0057 0.0001 0 0 0 0 0
Mitochondria 0.0003 0.0003 0.0002 0.0002 0.0003 0.0002 0.0021 0.0008 0.0062 0.0058 0.0155 0.0008 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
None 0.0701 0.021 0.0721 0.0565 0.0396 0.0343 0.0519 0.0193 0.038 0.0594 0.0145 0.0377 0.0933 0.0472 0.0486 0.0625 0.0214 0.0511
Nuclear Periphery 0.002 0.0033 0.002 0.0025 0.001 0.003 0.0067 0.003 0.0061 0.0061 0.0182 0.0042 0.0013 0.0007 0.0008 0.0015 0.0007 0.0006
Nucleolus 0.0001 0.0006 0.0001 0.0001 0 0.0002 0.0004 0.0004 0.0006 0.0006 0.0017 0.0006 0 0 0 0 0 0
Nucleus 0.0061 0.0102 0.0174 0.0297 0.0074 0.0178 0.0184 0.017 0.0209 0.0129 0.0277 0.024 0.0068 0.0026 0.0054 0.0272 0.0045 0.0048
Peroxisomes 0.0004 0.0004 0.0002 0.0001 0 0.0002 0.0035 0.0008 0.0045 0.0072 0.0133 0.0073 0.0001 0 0.0001 0 0 0.0001
Punctate Nuclear 0.0014 0.0052 0.0015 0.0012 0.0006 0.0053 0.0101 0.0098 0.0112 0.02 0.0125 0.0242 0.0008 0.0005 0.0005 0.0006 0.0004 0.0005
Vacuole 0.0013 0.0014 0.0011 0.0018 0.0021 0.0014 0.0022 0.001 0.0024 0.002 0.0017 0.0014 0.0005 0.0003 0.0005 0.0004 0.0003 0.0004
Vacuole Periphery 0.0002 0.0003 0.0002 0.0003 0.0003 0.0003 0.0006 0.0002 0.0006 0.0006 0.0011 0.0003 0.0001 0 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.2344 40.378 59.9244 66.015 56.4715 49.9804 43.3778 59.0462 69.0918 57.1187
Translational Efficiency 0.7034 1.1841 0.9492 0.9853 0.9848 0.7452 1.0436 0.8811 0.9536 0.9209

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Utr4

Utr4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utr4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available