Standard name
Human Ortholog
Description Subunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0.08 0 0.07 0.07 0.07 0.08 – 0.08 0.15 0.13 0.05 0 0 0.11 0.08 0.05 0.07 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0.07 – 0.1 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Golgi – 0.07 0 0 0.06 0.08 0.06 0.1 – 0.1 0 0.06 0.07 0.05 0.1 0.31 0.27 0.31 0 0 0 0 0 0
Mitochondria – 0 0.11 0.05 0.08 0.19 0.15 0.11 – 0.13 0.19 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0.05 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0 0 0 0.05 0 0.06 0.08 0.09
Nuclear Periphery – 0 0.11 0.24 0.11 0.13 0.19 0.16 – 0.16 0.15 0.15 0 0.11 0.14 0 0 0 0.05 0 0.09 0 0.05 0
Nucleolus – 0 0.06 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.9 0.82 0.87 0.83 0.66 0.68 0.61 – 0.61 0.58 0.44 0.9 0.92 0.93 0.85 0.87 0.88 0.63 0.86 0.8 0.84 0.79 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 1 1 2 19 – 17 4 9 0 0 0 2 0 0 3 0 0 0 1 0
Bud – 0 0 0 0 0 1 3 – 1 2 3 0 0 0 0 0 0 0 0 3 1 3 3
Bud Neck – 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site – 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 1 0 1 12 31 – 16 17 17 0 0 1 0 3 1 0 0 0 0 0 0
Cytoplasm – 2 12 11 29 42 57 61 – 47 51 67 9 15 18 33 18 11 3 0 3 1 6 4
Endoplasmic Reticulum – 0 0 2 1 18 24 54 – 60 18 48 2 0 0 11 8 6 1 0 0 1 1 1
Endosome – 0 0 0 1 1 1 0 – 0 0 1 0 0 0 0 0 0 4 1 0 1 1 2
Golgi – 6 3 11 25 46 46 78 – 60 13 30 14 21 53 89 62 70 1 0 0 0 1 0
Mitochondria – 0 17 14 32 107 114 85 – 80 65 131 1 1 3 4 9 9 0 0 3 1 2 3
Nucleus – 4 4 5 12 25 16 8 – 8 3 4 13 9 14 2 5 7 0 4 2 9 23 31
Nuclear Periphery – 1 17 73 46 76 144 119 – 103 52 78 1 47 71 12 10 5 2 3 15 5 13 8
Nucleolus – 2 9 14 10 17 15 16 – 2 4 2 2 6 6 7 5 11 0 2 1 1 5 14
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole – 0 0 0 1 1 2 1 – 3 1 0 0 0 0 0 0 0 0 0 1 0 1 0
Vac/Vac Membrane – 74 128 261 336 384 522 462 – 380 200 225 173 397 475 246 200 196 34 83 139 131 245 294
Unique Cell Count 82 156 301 405 578 773 761 625 347 509 193 431 512 289 231 223 55 97 175 156 309 368
Labelled Cell Count 89 190 392 494 719 957 937 778 430 615 215 496 641 406 320 317 55 97 175 156 309 368


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 33.8 18.7 20.5 19.3 16.9 16.7 17.0 – 17.1 15.8 15.8 24.5 26.8 28.1 35.3 34.2 36.6 26.6 27.8 29.0
Std Deviation (1e-4) – 7.3 6.5 5.6 5.8 5.4 5.1 4.6 – 4.5 4.3 4.6 6.4 7.3 7.1 10.6 10.3 10.9 8.0 9.0 9.4
Intensity Change (Log2) – – – 0.13 0.05 -0.14 -0.17 -0.14 – -0.13 -0.24 -0.24 0.39 0.52 0.59 0.92 0.87 0.97 0.51 0.57 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.6 – 0 2.8 0 0 0 0 0 0 0
Cytoplasm -1.9 -0.2 -0.2 -0.1 0.1 – -0.1 2.2 1.8 -1.2 -2.2 -2.2 1.2 0 -1.1
Endoplasmic Reticulum 0 0 0 0 3.4 – 4.0 2.9 4.0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 2.1 2.7 2.1 3.3 – 3.1 0 2.0 2.3 1.6 3.3 7.2 6.4 7.2
Mitochondria -2.5 -1.1 2.3 1.3 0.1 – 0.6 2.2 3.9 -4.4 0 0 -4.5 -2.7 -2.6
Nucleus 0 0 1.0 0 0 – 0 0 0 1.8 0 0 0 0 0
Nuclear Periphery 3.4 0.2 0.8 2.3 1.5 – 1.7 1.2 1.4 -4.4 0 1.0 -2.8 -2.5 -3.5
Nucleolus -0.5 -1.9 -1.7 0 0 – 0 0 0 0 0 0 -1.8 -1.9 -0.4
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 1.3 0.3 -3.8 -3.6 -5.1 – -5.0 -5.3 -8.3 2.0 3.5 4.0 0.8 1.2 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.861 24.1622 22.5553 22.4021 19.519 22.693 20.7999 25.2143 23.2511 22.9605 21.2429 22.2702 21.9817 26.5133 24.1243 22.9785 21.8239 24.2889
Actin 0.001 0 0.0011 0 0.0035 0 0.0007 0 0.0008 0 0 0.0007 0.0074 0 0 0 0.0001 0
Bud 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0.0007 0 0.0002 0.0001 0.0002 0 0.0001 0 0.0001 0
Bud Neck 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0005 0.0002 0 0.0001 0.0001 0 0
Bud Periphery 0.0004 0.0001 0.0001 0 0.0004 0.0001 0.0006 0 0.0015 0 0.0007 0.0005 0.0005 0.0001 0.0004 0.0001 0.0001 0.0001
Bud Site 0.0004 0 0.0001 0 0.0004 0.0001 0.0004 0 0.0004 0 0 0.0001 0.0002 0 0.0001 0 0.0001 0
Cell Periphery 0.0009 0.0004 0.0008 0.0004 0.0005 0.0002 0.0011 0.0002 0.0012 0.0003 0.0007 0.0005 0.0012 0.0005 0.0014 0.0004 0.0003 0.0002
Cytoplasm 0.002 0.0004 0.0003 0.0001 0.0001 0.0001 0.0009 0.0002 0.0004 0.0001 0.0001 0.0006 0.0001 0.0002 0.0005 0.0001 0.0012 0
Cytoplasmic Foci 0.0093 0 0.0004 0 0.0014 0.0014 0.0063 0 0.0031 0 0 0.0003 0.0003 0 0.0025 0 0.0111 0
Eisosomes 0 0 0 0 0.0001 0 0.0002 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0009 0.0003 0.0003 0.0001 0.0003 0.0001 0.0024 0.0002 0.0003 0 0.0004 0.0026 0.0002 0.0003 0.0003 0.0001 0.0013 0.0001
Endosome 0.0111 0.0021 0.0053 0.0019 0.0053 0.002 0.0089 0.0026 0.0077 0.0017 0.0036 0.0042 0.0057 0.0034 0.006 0.0028 0.0098 0.001
Golgi 0.0011 0 0.0006 0 0.002 0.0001 0.0005 0 0.0017 0 0 0.0002 0.0013 0.0001 0.0004 0.0002 0.0011 0.0001
Lipid Particles 0.0066 0.0001 0.0016 0 0.008 0.002 0.0029 0 0.0016 0 0.0002 0.0003 0.0011 0.0001 0.0008 0.0002 0.0374 0.0014
Mitochondria 0.0055 0.002 0.0031 0.0003 0.0062 0.0003 0.0087 0.0001 0.0048 0 0.0003 0.0018 0.009 0.006 0.0076 0.0005 0.0015 0.001
None 0.0025 0 0.0001 0 0.0002 0 0.0009 0 0.0002 0 0 0.0003 0 0 0.0002 0 0.0009 0
Nuclear Periphery 0.0129 0.0172 0.0121 0.0018 0.003 0.0181 0.0162 0.0073 0.0061 0.0014 0.0085 0.0127 0.0079 0.0096 0.01 0.0091 0.006 0.0153
Nucleolus 0.0024 0.0002 0.0006 0.0008 0.0008 0.0027 0.0012 0.0001 0.0026 0.0001 0.0003 0.0003 0.0013 0.0003 0.0031 0.0003 0.0007 0.0003
Nucleus 0.003 0.0007 0.0009 0.0006 0.0013 0.0008 0.0032 0.0003 0.0019 0.0002 0.0007 0.0008 0.0019 0.0004 0.0073 0.0008 0.0009 0.0004
Peroxisomes 0.0039 0 0.0007 0 0.0054 0.0001 0.001 0 0.0022 0 0 0 0.0002 0 0.0001 0 0.0006 0
Punctate Nuclear 0.0051 0 0.0001 0 0.0002 0.0001 0.0009 0 0.0013 0 0 0.0001 0 0 0.0013 0 0.0014 0
Vacuole 0.2667 0.2676 0.2897 0.2599 0.271 0.1893 0.2705 0.236 0.2907 0.2584 0.2115 0.1568 0.2891 0.2691 0.2879 0.2646 0.3002 0.1094
Vacuole Periphery 0.664 0.7088 0.6819 0.7339 0.6897 0.7822 0.6721 0.7528 0.6708 0.7377 0.7725 0.8166 0.6719 0.7099 0.67 0.7205 0.6254 0.8707

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 196.7809 212.3715 204.106 224.1058 228.2299 198.8175 221.5197 242.7097 229.4592 217.5305
Translational Efficiency 1.7458 1.705 1.6745 1.5001 1.5268 1.5731 1.5729 1.2867 1.4981 1.5361

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
Localization
Cell Percentages vacuolar membrane (95%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vma8

Vma8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available