Standard name
Human Ortholog
Description DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.14 0.11 0.12 0.13 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.15 0.13 0.38 0.3 0.35 0.49 0.52 0.55 0.48 0 0 0.05 0.05 0 0 0 0 0 0 0 0
Nucleus 0.53 0.53 0.47 0.22 0.37 0.34 0.26 0.32 0.28 0.25 0.29 0.22 0.18 0.29 0.3 0.3 0.25 0.28 0.27 0.39 0.28 0.25 0.12 0.11
Nuclear Periphery 0.05 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0 0.05
Nucleolus 0.38 0.47 0.48 0.78 0.57 0.55 0.62 0.59 0.57 0.57 0.56 0.66 0.67 0.57 0.62 0.47 0.47 0.49 0.6 0.5 0.54 0.55 0.58 0.48
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0 0.06 0.08 0.07 0 0.09 0.07 0.11 0.06 0.05 0 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.13 0.13 0.05 0.06 0 0.1 0.12 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 1 1 11 9 9 3 5 0 0 1 0 0 0 2 4 2 0 3 1
Bud Neck 0 0 1 0 0 0 1 1 1 1 1 0 0 0 1 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 2 3 9 10 17 16 28 14 25 1 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 4 8 7 9 19 18 11 8 11 3 5 34 34 28 35 28 21 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 10 6 7 0 0 1 0 1 0
Endosome 2 2 6 1 12 6 15 13 0 6 4 13 8 10 2 6 5 3 2 3 7 5 10 15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 6 4 3 1 2 0 0 1
Mitochondria 0 12 35 37 53 175 181 213 226 280 184 219 1 3 12 14 7 11 0 3 8 1 10 10
Nucleus 73 214 199 57 148 155 155 199 131 136 96 101 41 68 77 91 54 69 46 181 141 41 30 31
Nuclear Periphery 7 8 20 3 10 9 17 11 9 16 14 21 1 0 2 5 2 4 4 2 31 0 8 15
Nucleolus 53 190 200 197 226 256 368 364 264 305 187 300 155 136 160 144 104 121 103 234 269 91 146 142
Peroxisomes 1 0 0 0 1 2 1 0 1 0 0 2 4 6 2 0 1 1 0 0 1 0 0 0
SpindlePole 1 2 3 17 16 27 48 44 17 46 22 52 13 12 6 7 6 3 1 1 5 4 7 18
Vac/Vac Membrane 8 13 9 2 4 8 4 4 2 4 3 3 2 2 2 37 28 31 7 28 22 16 30 48
Unique Cell Count 139 401 420 254 396 462 596 616 460 534 333 457 232 238 260 304 219 245 173 464 499 166 255 294
Labelled Cell Count 151 445 482 324 483 667 819 888 684 842 531 746 260 271 295 351 247 275 173 464 499 166 255 294


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.0 10.3 7.4 7.0 6.8 5.5 5.4 5.4 4.8 4.5 4.5 4.5 11.1 10.3 8.7 16.5 18.5 17.5 12.1 12.1 12.2
Std Deviation (1e-4) 2.8 2.3 1.6 1.5 1.6 1.4 1.4 1.3 1.2 1.2 1.1 1.2 4.2 3.7 2.7 8.5 9.8 8.0 2.9 2.6 2.6
Intensity Change (Log2) – – – -0.09 -0.14 -0.43 -0.47 -0.47 -0.64 -0.72 -0.74 -0.72 0.58 0.47 0.22 1.15 1.31 1.23 0.7 0.7 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 3.4 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.4 1.9 1.1 -0.1 -0.2 0.2 0 -1.0 6.3 6.2 5.0 5.4 5.7 4.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.6 -0.2 1.2 0.8 0 0 0 0 0 2.2 0 0.6 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.5 2.3 10.3 8.5 9.7 0 0 0 0 -4.2 -3.7 -1.9 -2.0 -2.5 -1.9
Nucleus -6.5 -2.9 -4.2 -7.0 -4.9 -5.8 -7.0 -5.2 -7.9 -7.5 -4.7 -4.6 -4.7 -5.6 -4.9
Nuclear Periphery -2.5 -1.7 -2.3 -1.6 -2.8 0 0 0 0 -3.0 -3.4 -2.9 -2.3 -2.5 -2.1
Nucleolus 7.7 2.7 2.3 4.5 3.6 0 0 0 0 4.7 2.3 3.5 -0.1 0 0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 4.4 3.1 4.2 5.3 4.9 0 0 0 0 3.9 3.6 0 0 0 0
Vacuole 0 -1.3 -0.4 -2.1 -2.1 -2.3 -1.8 -1.4 -1.9 0 0 0 5.5 5.5 5.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4592 10.1371 9.0517 8.7129 6.5136 8.9473 11.1112 12.1978 11.6093 10.0089 10.9106 11.4013 13.0586 12.6436 11.1661 11.6715 10.5842 10.9921
Actin 0.0226 0 0.012 0.0008 0.0575 0.0009 0.0391 0 0.0062 0.0063 0.0078 0.0017 0 0 0 0.0002 0 0
Bud 0.0003 0 0.0001 0.0002 0.0005 0.0001 0.0005 0 0.0003 0.0006 0.0009 0.0003 0 0.0001 0 0.0002 0 0
Bud Neck 0.0005 0.0002 0.0002 0.0003 0.0035 0.0006 0.0009 0.0001 0.0005 0.0006 0.0007 0.0007 0.0002 0.0003 0.0001 0.0002 0.0002 0.0002
Bud Periphery 0.0005 0 0.0002 0.0009 0.0008 0.0002 0.0009 0.0001 0.0003 0.0008 0.0023 0.0008 0.0001 0.0001 0 0.0008 0.0001 0.0002
Bud Site 0.0005 0.0001 0.0012 0.0002 0.0022 0.0002 0.0023 0.0001 0.0044 0.0014 0.002 0.0013 0.0001 0.0004 0.0001 0.0004 0.0001 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0.0003 0.0004 0.0003 0.0005 0.0001 0.0003 0.0001 0.0008 0.0003 0.0001 0.0003 0 0.0003 0.0001 0.0001
Cytoplasm 0.0033 0 0 0.0001 0.001 0.0018 0.0056 0 0.0055 0.0058 0.0008 0.0005 0 0 0 0 0 0
Cytoplasmic Foci 0.0049 0.0001 0.0007 0.0001 0.0042 0.0013 0.0155 0 0.0051 0.0047 0.0021 0.0061 0.0013 0.0001 0.0002 0.0002 0 0.0001
Eisosomes 0.0001 0 0.0001 0 0.0006 0 0.0002 0 0 0 0.0002 0.0001 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0026 0 0 0.0002 0.0012 0.0003 0.01 0 0.0005 0.002 0.0011 0.0005 0 0 0 0 0 0
Endosome 0.0082 0 0.0005 0.0002 0.0107 0.0006 0.0264 0 0.0023 0.0397 0.0116 0.004 0.0002 0.0002 0 0.0002 0 0.0001
Golgi 0.0028 0 0.0013 0 0.0072 0.0004 0.0063 0 0.0014 0.0026 0.0056 0.0018 0.0001 0 0 0.0002 0 0
Lipid Particles 0.009 0.0003 0.0019 0.0005 0.0104 0.0042 0.0126 0 0.0007 0.0019 0.0093 0.0133 0.0043 0.0003 0.0012 0.0033 0.0001 0.0007
Mitochondria 0.0032 0.0001 0.0033 0.0009 0.0336 0.0077 0.0092 0.0001 0.0007 0.0032 0.0642 0.0173 0.0003 0.0002 0 0.0106 0.0007 0.0005
None 0.005 0 0 0.0001 0.0008 0.001 0.0027 0 0.0001 0.0029 0.0006 0.0003 0 0 0 0.0003 0 0
Nuclear Periphery 0.0217 0.002 0.0006 0.0236 0.0234 0.004 0.0223 0.0003 0.002 0.0146 0.0396 0.016 0.0003 0.0006 0.0003 0.0028 0.0028 0.0115
Nucleolus 0.6448 0.6749 0.7368 0.763 0.521 0.7239 0.525 0.6634 0.7084 0.6235 0.5145 0.677 0.7926 0.7012 0.841 0.7404 0.5332 0.7651
Nucleus 0.226 0.3112 0.2351 0.2031 0.3028 0.2425 0.2643 0.3321 0.2586 0.2539 0.303 0.2372 0.196 0.2744 0.1532 0.2329 0.4476 0.2173
Peroxisomes 0.0005 0 0.0047 0 0.0105 0.0005 0.0076 0 0.0009 0.0015 0.0021 0.0015 0.0001 0 0 0.0005 0 0
Punctate Nuclear 0.0391 0.0104 0.0005 0.001 0.0042 0.0074 0.0389 0.002 0.0012 0.0157 0.0113 0.0105 0.0032 0.0122 0.0035 0.0032 0.0145 0.0028
Vacuole 0.0014 0.0002 0.0003 0.0013 0.0017 0.0009 0.0058 0.001 0.0004 0.0134 0.0119 0.0052 0.0005 0.0055 0.0001 0.0012 0.0001 0.0004
Vacuole Periphery 0.0028 0.0003 0.0003 0.0033 0.0017 0.0011 0.0036 0.0005 0.0003 0.0047 0.0078 0.0037 0.0004 0.0038 0.0001 0.0018 0.0003 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.8956 63.9586 61.7696 57.3016 70.3885 52.822 48.4201 43.5372 45.4521 61.415
Translational Efficiency 0.9707 0.9253 0.7962 0.8466 0.7362 0.8812 0.8793 0.7032 0.7045 0.7502

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
Localization
Cell Percentages nucleolus (27%), nucleus (26%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pol5

Pol5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available