Standard name
Human Ortholog
Description Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity; serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0.06 0.05 0.1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0.12 0.13 0.05 0 – 0 0 0 0 0.05 0.13 0.12 – – 0 0 0 0 0 0 0
Cytoplasm 0.86 0.87 0.74 0.86 0.53 0.6 0.71 – 0.61 0.53 0.74 0.71 1.0 0.83 0.77 – – 0.76 0.25 0.42 0.27 0.43 0.64 0.47
Endoplasmic Reticulum 0.14 0.14 0.05 0.09 0.52 0.33 0.07 – 0 0 0 0 0 0.2 0.25 – – 0.05 0.53 0.39 0.37 0.12 0.09 0.06
Endosome 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0.06 0 0 0.14
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0.08
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.1 0 0 0.05 0.16 – 0.41 0.44 0.23 0.31 0 0 0 – – 0.19 0.08 0.08 0.07 0.24 0.1 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 3 4 – 1 2 1 4 0 1 2 – – 0 3 3 2 1 4 4
Bud 0 1 0 1 1 2 6 – 2 2 4 4 0 0 1 – – 0 3 4 1 6 9 21
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 1 5 2 2 2 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 5 1 10 10 6 5 – 0 1 2 2 1 26 20 – – 0 0 0 1 0 1 0
Cytoplasm 74 211 31 73 42 78 100 – 39 72 94 143 20 162 128 – – 16 25 102 24 44 134 100
Endoplasmic Reticulum 12 35 2 8 41 43 10 – 0 2 3 7 0 39 42 – – 1 54 96 32 12 18 12
Endosome 0 2 2 0 0 0 3 – 2 3 2 3 0 0 1 – – 0 3 5 5 4 7 29
Golgi 0 0 1 1 0 3 5 – 2 1 1 3 0 1 0 – – 1 0 2 1 1 3 16
Mitochondria 1 0 0 0 0 1 1 – 0 1 2 0 0 0 0 – – 1 1 1 3 1 2 3
Nucleus 0 0 1 0 0 0 0 – 1 4 1 4 0 0 0 – – 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 – – 0 1 0 0 0 0 0
Nucleolus 0 0 2 1 0 1 2 – 1 3 1 0 0 0 0 – – 1 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 1 0 0 0 0 0 1
SpindlePole 1 1 1 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 1 1 3 0 3
Vac/Vac Membrane 2 4 4 3 1 6 23 – 26 60 29 62 0 2 1 – – 4 8 19 6 24 20 11
Unique Cell Count 86 242 42 85 79 129 141 64 137 127 202 20 196 167 21 104 245 89 104 209 213
Labelled Cell Count 90 259 45 98 96 143 159 74 152 140 232 21 231 195 25 104 245 89 104 209 213


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 10.9 9.5 13.5 29.0 25.0 17.6 – 13.6 12.4 11.8 11.3 11.5 14.5 15.1 – – 25.2 7.4 10.1 13.2
Std Deviation (1e-4) 3.0 2.6 2.7 4.6 7.9 7.1 5.8 – 5.1 6.2 6.1 3.9 3.9 4.5 6.3 – – 13.7 1.9 2.9 4.6
Intensity Change (Log2) – – – 0.51 1.61 1.4 0.89 – 0.53 0.39 0.31 0.25 0.28 0.61 0.67 – – 1.41 -0.36 0.1 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Cytoplasm 1.7 -2.2 -1.6 -0.4 – -1.4 -2.4 0 -0.4 2.5 1.3 0.4 – – 0.2
Endoplasmic Reticulum 0 5.2 3.7 0 – 0 0 0 0 0 2.4 2.9 – – 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – – 0
Vacuole 0 0 0 1.1 – 3.5 4.1 1.9 2.8 0 0 0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8756 3.3021 2.5612 2.4373 1.1226 2.7771 2.5203 5.5818 3.6599 1.8622 2.7364 3.334 2.3687 3.2375 2.4385 2.6546 3.6849 4.0757
Actin 0.0254 0.002 0.0502 0.0008 0.0023 0.0013 0.0258 0.0005 0.0011 0.0002 0.0094 0.0013 0.028 0.0042 0.0207 0.1042 0.0001 0.001
Bud 0.0004 0.0111 0.0004 0.0001 0.0005 0.0003 0.0012 0.0002 0.0003 0.0002 0.0005 0.0004 0.001 0.0005 0.0005 0.001 0.0003 0.0001
Bud Neck 0.0009 0.0006 0.001 0.0003 0.0007 0.0008 0.0059 0.001 0.001 0.0011 0.0005 0.003 0.0017 0.0005 0.001 0.0062 0.0004 0.0008
Bud Periphery 0.0005 0.0021 0.0005 0.0001 0.0013 0.0006 0.002 0.0003 0.0004 0.0002 0.0007 0.0004 0.0013 0.0007 0.0006 0.0012 0.0001 0.0002
Bud Site 0.0025 0.0099 0.0037 0.0001 0.0007 0.0002 0.0075 0.0051 0.0044 0.0003 0.0007 0.0007 0.0099 0.002 0.0025 0.0169 0 0.0001
Cell Periphery 0.0015 0.0019 0.0011 0.0004 0.0005 0.0003 0.0019 0.0018 0.0013 0.0003 0.0002 0.0005 0.0012 0.0013 0.0009 0.0012 0.0002 0.0003
Cytoplasm 0.4274 0.5175 0.4351 0.5036 0.3748 0.6095 0.3988 0.542 0.5401 0.3642 0.4537 0.512 0.4191 0.6189 0.5357 0.5978 0.8011 0.4256
Cytoplasmic Foci 0.0355 0.0561 0.0478 0.0564 0.1225 0.029 0.0368 0.0252 0.0241 0.0716 0.0735 0.0494 0.0495 0.0379 0.0282 0.0174 0.0072 0.0267
Eisosomes 0.0004 0.0001 0.0003 0 0.0001 0 0.0004 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0018 0 0
Endoplasmic Reticulum 0.2343 0.1816 0.1699 0.1652 0.1534 0.1817 0.193 0.1369 0.1717 0.191 0.0564 0.1385 0.1887 0.0724 0.1394 0.1617 0.1453 0.2003
Endosome 0.0738 0.0902 0.1255 0.1787 0.1273 0.0897 0.083 0.0579 0.1082 0.1664 0.1344 0.0941 0.0853 0.0969 0.0762 0.0269 0.0223 0.1228
Golgi 0.018 0.0027 0.0211 0.003 0.0209 0.0057 0.0224 0.0025 0.0045 0.0029 0.0713 0.0153 0.0118 0.0054 0.0262 0.0087 0.0004 0.0111
Lipid Particles 0.0269 0.0168 0.0249 0.0052 0.0335 0.0168 0.0201 0.0061 0.0078 0.008 0.0576 0.0042 0.0652 0.0132 0.0067 0.0094 0.0015 0.0033
Mitochondria 0.0174 0.0007 0.0078 0.0013 0.0342 0.0058 0.0093 0.0004 0.0036 0.0005 0.0377 0.0024 0.0052 0.0018 0.0046 0.0018 0.0002 0.0186
None 0.0487 0.0302 0.0586 0.0391 0.0827 0.0126 0.1096 0.0907 0.017 0.0464 0.0382 0.0167 0.0672 0.0699 0.0704 0.0149 0.004 0.0423
Nuclear Periphery 0.015 0.011 0.0083 0.0137 0.0108 0.0232 0.0192 0.0056 0.012 0.0155 0.0103 0.0067 0.0107 0.0071 0.0084 0.0113 0.0057 0.0315
Nucleolus 0.0025 0.0004 0.0002 0.0001 0.0003 0.0001 0.0017 0.0003 0.0001 0.0002 0.0002 0.0002 0.0005 0.0002 0.0002 0.0011 0 0.0001
Nucleus 0.0049 0.0028 0.0026 0.0019 0.0021 0.0023 0.0039 0.0035 0.0038 0.0027 0.002 0.0038 0.004 0.0023 0.0023 0.0033 0.0024 0.0058
Peroxisomes 0.0092 0.0003 0.0063 0.0019 0.0051 0.0002 0.0064 0.0004 0.0002 0.0009 0.0081 0.001 0.0076 0.0004 0.0142 0.004 0 0.0009
Punctate Nuclear 0.0111 0.0011 0.0034 0.0016 0.0022 0.0006 0.0057 0.0007 0.0008 0.0023 0.0012 0.001 0.003 0.0008 0.0036 0.0055 0.0003 0.0005
Vacuole 0.0409 0.0585 0.0266 0.0212 0.019 0.0123 0.0411 0.1167 0.0926 0.1149 0.0373 0.1413 0.033 0.0608 0.0493 0.0028 0.0076 0.0914
Vacuole Periphery 0.0028 0.0022 0.0046 0.0053 0.0053 0.007 0.0044 0.0022 0.005 0.0103 0.0059 0.0073 0.0057 0.0029 0.0082 0.0008 0.0009 0.0164

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 151.2266 100.2919 86.3129 90.4546 63.7327 226.5698 122.7053 122.5951 164.5419 105.9184
Translational Efficiency 1.5308 1.2138 1.2335 1.8745 1.6443 1.4478 1.5801 2.0494 1.5395 1.5577

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar proteinase B (yscB) with H3 N-terminal endopeptidase activity; serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; PRB1 has a paralog, YSP3, that arose from the whole genome duplication
Localization
Cell Percentages ER (59%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Prb1

Prb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available