Standard name
Human Ortholog
Description Ferrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0 0.05 0.05 0.07 0.06 0.08 0.1 0.1 0.09 0.11 0.11 0.05 0.05 0.05 0.09 0.05 0.1 0 0 0 0.17 0.19 0.21
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.11 0 0.05 0 0 0 0 0 0 0 0 0 0.19 0.18 0.15 0 0 0 0.12 0 0 0.06 0.05 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.09 0.1 0.07 0.07 0.1 0.06 0 0.08 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Nucleus 0.18 0.17 0.29 0.42 0.22 0.33 0.33 0.27 0.33 0.28 0.29 0.26 0.1 0.11 0.2 0.5 0.53 0.39 0.05 0.07 0.06 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.75 0.63 0.61 0.72 0.64 0.65 0.73 0.67 0.76 0.73 0.74 0.72 0.7 0.71 0.46 0.51 0.55 0.69 0.77 0.8 0.61 0.64 0.48
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 0 0 0 0 1 3 1 0 0 0 0 0 2 0 0 1
Bud 0 0 1 0 1 1 3 2 1 1 3 0 0 0 0 0 0 0 15 15 10 4 4 10
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 1 1 0 1 1 0 0 2 2 1 0 0 0 0 0 0 0 0
Cytoplasm 58 13 29 23 43 42 81 121 82 85 91 90 13 22 26 30 21 24 12 0 1 27 31 37
Endoplasmic Reticulum 0 1 0 0 2 2 0 7 2 1 1 2 0 1 1 3 8 7 0 0 0 0 0 3
Endosome 37 11 29 1 17 4 4 3 0 1 1 0 52 87 72 6 4 3 44 27 22 9 7 13
Golgi 2 4 3 0 1 0 0 1 0 0 0 0 11 17 6 5 3 6 1 4 0 1 0 1
Mitochondria 19 55 52 30 40 64 61 43 62 50 52 45 2 3 2 6 8 4 2 12 8 4 2 8
Nucleus 61 108 152 184 129 219 329 337 266 261 248 213 27 52 95 175 208 96 18 47 32 0 1 1
Nuclear Periphery 5 12 17 6 11 9 11 4 4 8 5 2 1 2 2 2 3 2 2 9 15 3 2 3
Nucleolus 20 46 20 4 5 14 13 20 5 6 6 4 29 45 15 17 8 5 7 24 14 0 0 0
Peroxisomes 0 0 0 0 1 0 1 0 0 1 1 0 1 0 1 0 0 0 0 1 0 0 0 1
SpindlePole 7 2 6 1 7 1 0 1 1 2 0 0 6 29 10 4 0 1 7 12 5 6 2 5
Vac/Vac Membrane 207 465 337 267 422 428 643 895 531 695 624 607 192 333 342 161 200 135 261 527 476 98 103 84
Unique Cell Count 348 619 533 438 588 671 996 1228 798 916 860 820 268 473 484 350 396 244 378 684 594 161 161 176
Labelled Cell Count 417 717 647 516 680 784 1147 1436 954 1112 1033 963 335 596 576 410 463 283 378 684 594 161 161 176


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 18.4 12.0 12.7 12.4 12.8 14.0 15.9 15.5 16.5 17.0 17.6 15.2 13.4 11.7 17.5 22.2 26.3 7.3 6.8 6.9
Std Deviation (1e-4) 5.0 8.5 6.1 6.2 6.4 7.6 8.3 8.4 9.2 8.9 9.5 9.5 5.4 4.4 3.7 8.2 10.1 12.3 8.0 2.4 2.9
Intensity Change (Log2) – – – 0.08 0.05 0.1 0.22 0.41 0.38 0.47 0.51 0.56 0.34 0.16 -0.03 0.55 0.89 1.14 -0.72 -0.83 -0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 1.3 0.6 1.9 3.0 3.1 2.6 3.3 3.5 -0.4 -0.6 0 1.8 -0.1 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -4.7 -2.1 -5.1 -6.5 -7.5 -6.7 -6.9 -6.7 -6.8 6.2 6.4 5.0 -2.8 -3.6 -2.7
Golgi 0 0 0 0 0 0 0 0 0 0 3.4 0 0 0 0
Mitochondria -1.6 -1.8 -0.1 -2.6 -5.3 -1.3 -3.1 -2.6 -3.0 -4.8 -6.4 -6.6 -4.7 -4.7 -4.1
Nucleus 4.4 -2.5 1.5 1.8 -0.5 1.9 0 0.1 -1.0 -5.9 -6.9 -3.3 6.5 7.4 3.0
Nuclear Periphery -1.9 -1.4 -2.2 -2.9 -5.1 -3.9 -3.3 -3.8 -4.5 -2.5 -3.2 -3.3 -2.6 -2.5 -2.0
Nucleolus -2.8 -3.3 -1.7 -3.1 -2.7 -4.1 -4.3 -4.1 -4.4 3.9 3.7 -0.6 0.8 -1.5 -1.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.1 0 0 0 0 0 0 0 0 4.3 1.2 0 0 0
Vacuole -0.7 3.1 0.2 0.5 4.1 1.2 5.1 3.7 4.2 2.4 2.4 2.5 -5.0 -3.9 -2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4868 14.9982 11.698 10.7457 11.402 14.2096 6.4239 7.9099 6.7626 6.6227 7.8851 8.7305 12.018 19.0053 14.9417 13.4214 13.1795 15.2846
Actin 0.0057 0 0.0002 0 0.0004 0 0.002 0.0007 0.0003 0.0001 0 0 0.0104 0.0001 0.0001 0 0.0006 0.0001
Bud 0.0002 0.0003 0.0012 0.0002 0.0037 0.0002 0.0009 0.0156 0.0117 0.008 0.0003 0.0008 0.0006 0.0004 0.0008 0.0002 0.0002 0.0002
Bud Neck 0.0004 0.0005 0.0015 0.0004 0.0006 0.0005 0.0012 0.0034 0.0049 0.002 0.0017 0.0013 0.0019 0.0007 0.0006 0.0007 0.0007 0.0005
Bud Periphery 0.0006 0.0008 0.0018 0.0004 0.0045 0.0006 0.002 0.0169 0.0132 0.0312 0.0008 0.0035 0.0009 0.0012 0.0015 0.0005 0.0005 0.0006
Bud Site 0.0004 0.0005 0.0026 0.0002 0.0014 0.0002 0.0014 0.039 0.0218 0.0018 0.0007 0.0006 0.0052 0.0014 0.0009 0.0004 0.0006 0.0003
Cell Periphery 0.0087 0.0113 0.0108 0.0061 0.0093 0.01 0.005 0.0085 0.0051 0.0034 0.0033 0.0056 0.0098 0.0154 0.0142 0.0049 0.0092 0.01
Cytoplasm 0.0006 0.0013 0.0024 0.0017 0.0032 0.0004 0.0029 0.0086 0.0053 0.0206 0.0027 0.0017 0.0017 0.0004 0.0006 0.0009 0.0004 0.0003
Cytoplasmic Foci 0.0047 0.0027 0.0153 0.0041 0.0074 0.0031 0.0287 0.0289 0.0244 0.0217 0.091 0.0199 0.0118 0.0043 0.009 0.0189 0.0049 0.0046
Eisosomes 0 0 0 0 0.0001 0 0.0001 0.0002 0 0 0 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0005 0.0004 0.0006 0.0003 0.0007 0.0005 0.0031 0.003 0.0021 0.002 0.0013 0.0014 0.0025 0.0005 0.0006 0.0002 0.0005 0.0006
Endosome 0.0544 0.0197 0.0491 0.1027 0.0492 0.05 0.2394 0.1722 0.2217 0.2498 0.2974 0.1767 0.0733 0.0155 0.0737 0.0589 0.1134 0.0396
Golgi 0.003 0.0009 0.0048 0.0032 0.0056 0.0042 0.0309 0.015 0.0132 0.0118 0.0241 0.009 0.0112 0.002 0.011 0.0057 0.0125 0.0045
Lipid Particles 0.0289 0.014 0.0286 0.0064 0.036 0.0275 0.1112 0.0443 0.04 0.0758 0.0929 0.0675 0.0948 0.0338 0.0425 0.0579 0.122 0.0887
Mitochondria 0.0372 0.0029 0.0355 0.0066 0.0131 0.009 0.029 0.0391 0.1126 0.0187 0.0034 0.0232 0.0393 0.0229 0.1157 0.1041 0.04 0.0397
None 0.0004 0 0.0068 0 0.0081 0.0001 0.0027 0.0073 0.0183 0.0165 0.0003 0.0004 0.003 0.0001 0.0001 0.0001 0.0003 0.0001
Nuclear Periphery 0.0025 0.0032 0.0028 0.0016 0.001 0.0025 0.0029 0.0101 0.0079 0.0083 0.0014 0.0062 0.0091 0.0023 0.0036 0.0006 0.0032 0.0028
Nucleolus 0.0249 0.0233 0.022 0.0069 0.006 0.0127 0.0374 0.0473 0.0138 0.014 0.0049 0.0221 0.0164 0.0171 0.0091 0.0116 0.0379 0.0128
Nucleus 0.0081 0.0061 0.0042 0.0313 0.002 0.0057 0.0072 0.0136 0.0222 0.0079 0.0018 0.0055 0.0038 0.0031 0.0024 0.0027 0.004 0.0041
Peroxisomes 0.0049 0.0004 0.0018 0.0004 0.0014 0.0009 0.0079 0.0083 0.0051 0.0064 0.0139 0.0022 0.0021 0.0008 0.0026 0.0021 0.0037 0.0012
Punctate Nuclear 0.0005 0.0001 0.0013 0 0.0002 0 0.0005 0.0019 0.0016 0.0003 0.0005 0.0002 0.0031 0 0.0001 0.0001 0.0004 0.0001
Vacuole 0.4416 0.5495 0.4402 0.5216 0.4862 0.4572 0.2981 0.338 0.2625 0.2852 0.347 0.403 0.2782 0.4116 0.2974 0.3757 0.3577 0.3634
Vacuole Periphery 0.3717 0.3619 0.3664 0.3058 0.36 0.4148 0.1855 0.1781 0.1925 0.2146 0.1105 0.2491 0.4203 0.4664 0.4135 0.3537 0.2873 0.4259

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 159.634 188.7026 202.4745 198.1835 45.4885 178.247 336.2731 336.3849 294.2951 268.0381
Translational Efficiency 0.8974 1.0801 1.0845 0.7674 0.7909 1.2451 0.9431 0.7935 0.9565 0.7966

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ferrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
Localization
Cell Percentages cytoplasm (9%), nucleus (9%), mixed (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sit1

Sit1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sit1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available