Standard name
Human Ortholog
Description Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.31 0.38 0.34 0.48 0.32 0.31 0.31 0.38 – 0.49 0.63 0.58 0.62 0.86 0.96 – – 0.36 0 0.05 0 0.08 0.07 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Endosome 0.12 0 0.11 0 0.05 0 0 0 – 0 0 0 0 0 0 – – 0 0.06 0.05 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0.26 0.12 0 0.07 0.2 0.13 0.14 – 0.22 0.15 0.11 0 0 0 – – 0.06 0 0 0 0 0 0
Nucleus 0.06 0.06 0.07 0.05 0.1 0.13 0.16 0.1 – 0.06 0 0 0.05 0 0 – – 0.36 0 0.07 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.67 0.5 0.6 0.65 0.74 0.7 0.7 0.67 – 0.55 0.5 0.54 0.44 0.14 0.07 – – 0.39 0.82 0.7 0.85 0.78 0.77 0.72
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 2 0 0 0 1 0 – – 0 0 0 0 0 0 1
Bud 1 1 0 0 0 0 2 1 – 3 1 2 0 2 0 – – 0 3 7 8 9 7 7
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 1 0 0 1 0 0 3
Bud Site 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 3 – – 0 0 0 0 0 0 0
Cytoplasm 90 68 134 122 97 114 159 194 – 259 291 297 93 196 212 – – 54 11 10 12 19 20 36
Endoplasmic Reticulum 1 0 0 0 3 1 2 1 – 9 1 1 0 2 1 – – 9 0 0 1 2 3 3
Endosome 35 0 45 6 16 0 3 0 – 0 0 1 3 2 0 – – 0 17 10 17 4 8 15
Golgi 3 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 3 1 0 1 1 0
Mitochondria 6 46 48 10 22 73 67 72 – 116 68 58 0 0 0 – – 9 6 5 8 4 8 11
Nucleus 16 11 29 12 29 47 79 53 – 33 17 19 7 6 1 – – 55 3 14 3 2 3 0
Nuclear Periphery 0 0 1 1 0 1 2 0 – 7 2 8 0 0 0 – – 0 0 0 1 0 1 1
Nucleolus 2 1 4 0 0 0 1 2 – 0 1 1 0 0 0 – – 0 0 0 0 0 0 1
Peroxisomes 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 1 0 3 1 0 0 0 0 – 1 0 0 0 0 0 – – 1 2 6 3 2 7 4
Vac/Vac Membrane 191 89 236 166 222 258 356 341 – 288 233 272 65 32 16 – – 60 248 151 351 175 220 238
Unique Cell Count 286 177 394 255 300 370 509 509 526 463 508 149 228 220 152 301 216 415 225 285 331
Labelled Cell Count 347 217 500 318 389 494 671 664 718 614 659 168 241 233 189 301 216 415 225 285 331


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.0 4.9 5.5 6.3 5.5 5.6 5.5 – 4.9 5.5 5.3 5.9 5.5 5.6 4.8 4.8 6.3 6.4 6.5 6.6
Std Deviation (1e-4) 1.5 1.5 1.8 1.6 1.8 1.9 2.2 1.9 – 1.7 1.9 1.7 1.6 1.4 1.4 1.4 1.3 2.3 2.2 1.8 2.7
Intensity Change (Log2) – – – 0.17 0.38 0.18 0.21 0.19 – 0.02 0.17 0.13 0.29 0.19 0.21 -0.01 -0.0 0.38 0.4 0.43 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Cytoplasm 3.5 -0.5 -0.9 -0.9 1.3 – 4.6 8.4 7.3 6.0 12.5 14.9 – – 0.3
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Endosome -4.2 -2.8 -6.7 -7.2 -7.8 – -7.9 -7.5 -7.6 -3.4 -4.8 -5.2 – – -4.3
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Mitochondria -3.6 -2.1 2.9 0.4 0.9 – 3.9 1.1 -0.4 -4.5 -5.5 -5.4 – – -2.1
Nucleus -1.4 1.1 2.5 3.7 1.6 – -0.7 -2.4 -2.4 -1.1 -2.5 -3.8 – – 8.4
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – 0
Vacuole 1.3 3.9 2.8 3.1 2.2 – -1.6 -2.8 -1.9 -3.4 -11.1 -12.7 – – -4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7259 2.0561 2.4966 1.8637 1.6732 1.8539 3.6196 4.9711 4.3786 3.7667 3.2779 3.773 2.6595 2.3946 3.1592 1.9737 2.0945 2.7793
Actin 0.0222 0.0021 0.0026 0 0 0.0098 0.0521 0.0005 0.0143 0.0056 0.0032 0.0005 0.0001 0.0002 0.0357 0.0033 0 0
Bud 0.0011 0.0046 0.0005 0.0002 0.0003 0.0077 0.0037 0.0018 0.0037 0.0005 0.0005 0.0021 0.0002 0.0008 0.0003 0.0004 0.0004 0.0004
Bud Neck 0.0026 0.0015 0.0014 0.0013 0.0008 0.0044 0.0284 0.0041 0.0054 0.0014 0.0016 0.0057 0.0014 0.0011 0.001 0.0013 0.0004 0.0084
Bud Periphery 0.0013 0.0104 0.0008 0.0005 0.0005 0.0035 0.0038 0.0016 0.0076 0.0011 0.0015 0.0025 0.0003 0.0013 0.0005 0.0005 0.0003 0.0011
Bud Site 0.0214 0.0061 0.0026 0.0008 0.0007 0.0021 0.0085 0.0065 0.0106 0.0015 0.0022 0.0042 0.0047 0.0066 0.0011 0.001 0.0004 0.0014
Cell Periphery 0.0011 0.0035 0.0025 0.0029 0.0006 0.0029 0.0017 0.0023 0.0028 0.0017 0.0015 0.0029 0.0016 0.0036 0.0013 0.0005 0.0002 0.0022
Cytoplasm 0.0533 0.0709 0.0881 0.0038 0.005 0.0336 0.0376 0.0771 0.0155 0.0047 0.0055 0.0303 0.0117 0.1097 0.0541 0.1879 0.0834 0.0924
Cytoplasmic Foci 0.0333 0.0295 0.0225 0.0071 0.0054 0.0304 0.0417 0.0367 0.0334 0.0213 0.0297 0.0166 0.048 0.0726 0.0475 0.0197 0.0455 0.0217
Eisosomes 0.0002 0.0002 0.0001 0 0 0.0001 0.0011 0 0.0002 0.0002 0.0001 0 0 0.0002 0.0004 0.0001 0 0
Endoplasmic Reticulum 0.0114 0.0069 0.0117 0.0005 0.0006 0.003 0.014 0.0054 0.0062 0.0033 0.0041 0.0029 0.0022 0.0083 0.0048 0.003 0.0062 0.004
Endosome 0.3025 0.166 0.2759 0.2616 0.2304 0.2197 0.2178 0.201 0.2946 0.2434 0.3818 0.2198 0.3072 0.2164 0.2674 0.1956 0.2644 0.2035
Golgi 0.0163 0.0055 0.0115 0.0019 0.0032 0.005 0.0191 0.0082 0.0181 0.032 0.0078 0.0082 0.0091 0.0056 0.0281 0.0179 0.0015 0.0029
Lipid Particles 0.0452 0.0344 0.0312 0.0126 0.0063 0.0197 0.0322 0.0208 0.0393 0.0301 0.0167 0.0168 0.065 0.0343 0.0324 0.018 0.0046 0.0147
Mitochondria 0.006 0.0029 0.0249 0.0002 0.0003 0.0039 0.0246 0.0042 0.0232 0.0436 0.0285 0.0053 0.0096 0.0018 0.0966 0.0409 0.001 0.0375
None 0.1477 0.0977 0.0784 0.0001 0.0002 0.0279 0.0739 0.0475 0.0219 0.0056 0.0023 0.0103 0.0049 0.3728 0.0852 0.1529 0.2384 0.1683
Nuclear Periphery 0.0108 0.0083 0.0062 0.001 0.0013 0.0053 0.0212 0.0053 0.0185 0.0092 0.0314 0.0035 0.0043 0.0038 0.0062 0.0036 0.0034 0.0052
Nucleolus 0.0014 0.005 0.0033 0.0107 0.0015 0.0053 0.0085 0.0081 0.0057 0.0043 0.0026 0.006 0.0086 0.0012 0.001 0.001 0.0006 0.0055
Nucleus 0.0054 0.0069 0.0055 0.0024 0.0019 0.0089 0.0273 0.0113 0.005 0.0031 0.0058 0.0052 0.006 0.004 0.0021 0.0024 0.0038 0.0186
Peroxisomes 0.0225 0.0024 0.0016 0.0007 0.0004 0.003 0.0136 0.0018 0.0036 0.0036 0.0021 0.001 0.0032 0.0061 0.0064 0.0182 0.0002 0.0005
Punctate Nuclear 0.0074 0.0016 0.0008 0.0001 0.0001 0.0025 0.0117 0.0012 0.0049 0.0013 0.001 0.0007 0.011 0.0039 0.0006 0.0003 0.0003 0.001
Vacuole 0.2584 0.4731 0.3664 0.6328 0.6692 0.5295 0.2924 0.47 0.3736 0.5035 0.4142 0.5615 0.4231 0.1366 0.2816 0.2845 0.3044 0.3633
Vacuole Periphery 0.0284 0.0605 0.0617 0.0586 0.0713 0.0716 0.0652 0.0844 0.092 0.079 0.0558 0.0941 0.0777 0.0094 0.0456 0.0471 0.0408 0.0472

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.8608 18.9605 5.7557 13.1439 72.8118 35.6517 9.5855 13.3802 11.1289 63.4293
Translational Efficiency 1.1089 1.0277 0.8944 0.6985 0.9448 1.3325 1.0827 0.3386 0.4309 0.7628

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
Localization
Cell Percentages vacuole (68%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mnn1

Mnn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mnn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available