Standard name
Human Ortholog
Description Mannose-6-phosphate isomerase; catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.98 0.94 0.96 0.88 0.78 0.87 0.83 0.86 0.85 1.0 0.99 0.99 0.97 0.99 0.98 0.95 0.95 0.88 0.95 0.96 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.06 0.05 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0.06 0.14 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 5 3 6 0 0 0 0 0 0 0 1 0 2 0 0 0
Bud 1 0 0 1 0 0 0 3 1 2 0 1 1 0 0 2 0 0 3 5 1 2 4 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 1 1 2 2 3 8 4 9 13 6 10 0 3 1 3 3 3 2 0 0 0 0 0
Cytoplasm 311 202 158 258 222 347 373 320 287 326 237 310 367 595 591 455 308 230 304 197 142 208 444 432
Endoplasmic Reticulum 0 3 1 1 0 9 22 23 17 23 11 22 0 4 0 13 11 7 0 1 4 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 2 1 0 0
Golgi 0 0 0 0 0 0 0 2 2 0 1 0 0 0 0 0 0 0 0 0 2 0 2 0
Mitochondria 2 1 1 0 1 5 10 8 21 21 17 24 0 0 0 6 2 2 0 0 0 0 0 0
Nucleus 2 0 0 0 0 2 2 2 1 3 1 2 0 0 1 2 1 0 0 0 1 0 1 3
Nuclear Periphery 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 2 0 1 5 16 2 27 58 4 22 9 9 1 2 4 16 4 4 1 1 2 0 1 4
Unique Cell Count 316 206 159 264 235 361 425 411 329 393 277 365 368 601 594 470 310 234 320 209 163 220 461 461
Labelled Cell Count 325 207 162 267 241 369 443 424 347 413 289 378 369 604 597 497 330 246 320 209 163 220 461 461


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 18.0 14.7 14.6 16.3 16.8 16.2 17.0 18.4 18.1 18.6 19.6 19.1 19.7 19.2 18.1 24.3 24.4 14.0 13.3 13.2
Std Deviation (1e-4) 3.3 4.4 4.5 3.8 4.1 3.9 4.7 4.3 5.6 4.7 5.5 5.5 5.8 6.1 5.8 6.2 8.1 7.5 3.4 2.9 2.8
Intensity Change (Log2) – – – -0.01 0.15 0.19 0.14 0.21 0.32 0.3 0.34 0.41 0.38 0.42 0.38 0.3 0.72 0.73 -0.07 -0.15 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -2.6 -2.0 -4.3 -6.3 -4.4 -5.3 -4.8 -4.9 0.6 -0.4 0.2 -1.8 0 -0.9
Endoplasmic Reticulum 0 0 0 2.5 2.6 2.5 2.7 0 2.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.9 2.6 2.8 2.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.0 0 2.9 4.7 0 2.6 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.0913 13.0081 12.2236 11.8633 11.7403 12.9545 10.1777 14.5029 14.4639 14.846 13.1128 13.1788 14.6923 21.1129 18.9081 18.4737 17.5698 17.2217
Actin 0.0393 0.0005 0.0044 0.0003 0.0003 0.0001 0.0071 0.0007 0.0041 0.0336 0.0287 0.0007 0.004 0.0008 0.0022 0.0001 0.0009 0.0007
Bud 0.0009 0.0002 0.0006 0.0001 0.0002 0.0001 0.0007 0.0033 0.0002 0.0003 0.0002 0.0001 0.0008 0.0001 0.0007 0 0.0003 0.0002
Bud Neck 0.0035 0.0002 0.0008 0.0006 0.0007 0.0014 0.0005 0.0002 0.0018 0.0004 0.0007 0.0008 0.0005 0.0001 0.0003 0.0003 0.0004 0.0008
Bud Periphery 0.0006 0 0.0002 0 0 0 0.0009 0.0007 0.0001 0.0003 0.0002 0.0001 0.0005 0 0.0002 0 0.0001 0
Bud Site 0.004 0.0005 0.0009 0 0.0001 0 0.0006 0.0005 0.001 0.0007 0.0003 0.0001 0.0033 0.0003 0.0004 0 0.0001 0
Cell Periphery 0.0004 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.8164 0.9829 0.97 0.9849 0.9761 0.9747 0.9043 0.9769 0.9558 0.9159 0.907 0.9729 0.9385 0.9924 0.9751 0.992 0.9777 0.9775
Cytoplasmic Foci 0.0109 0.001 0.0031 0.0008 0.0013 0.0007 0.0199 0.0013 0.0087 0.0077 0.0099 0.0037 0.0078 0.0011 0.0029 0.0005 0.0012 0.0028
Eisosomes 0.0005 0 0 0 0 0 0.0001 0 0.0002 0.0001 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0074 0.0015 0.0012 0.0013 0.0021 0.001 0.0031 0.0019 0.0013 0.0005 0.0011 0.0016 0.0026 0.0004 0.0006 0.0008 0.0041 0.0011
Endosome 0.0171 0.0006 0.0032 0.0003 0.0008 0.0007 0.0082 0.0026 0.0036 0.0018 0.0033 0.0024 0.006 0.0002 0.0015 0.0002 0.0017 0.0006
Golgi 0.0037 0.0001 0.001 0 0.0001 0 0.0026 0.0002 0.0015 0.0038 0.004 0.0007 0.001 0.0001 0.0013 0 0.0001 0
Lipid Particles 0.0063 0 0.0008 0 0 0 0.0056 0.0002 0.0033 0.0007 0.0104 0.0009 0.0033 0 0.001 0 0.0001 0
Mitochondria 0.0038 0.0001 0.0014 0.0001 0.0001 0.0001 0.0095 0.0004 0.0005 0.0024 0.002 0.0035 0.0024 0.0001 0.0017 0 0.0002 0.0001
None 0.0104 0.0028 0.0012 0.0006 0.001 0.0005 0.0177 0.0017 0.0032 0.0012 0.0015 0.0012 0.0037 0.0008 0.0009 0.0004 0.0012 0.0012
Nuclear Periphery 0.0221 0.0016 0.0016 0.0015 0.0038 0.0024 0.0023 0.0036 0.0008 0.0005 0.0013 0.0011 0.0031 0.0004 0.0009 0.0008 0.0019 0.0015
Nucleolus 0.0019 0 0.0001 0 0 0 0.0002 0.0001 0.0004 0 0.0001 0 0.0002 0 0.0013 0 0 0
Nucleus 0.0287 0.0065 0.0072 0.0086 0.012 0.0167 0.0067 0.0044 0.0052 0.0035 0.0093 0.0075 0.0095 0.0027 0.0075 0.0044 0.009 0.0118
Peroxisomes 0.0026 0 0.0005 0 0 0 0.0051 0 0.0057 0.0258 0.0176 0.0015 0.0064 0 0.0005 0 0 0
Punctate Nuclear 0.0115 0.0002 0.0008 0.0003 0.0003 0.0001 0.0022 0.0004 0.0019 0.0005 0.0018 0.0006 0.0044 0.0001 0.0007 0.0001 0.0003 0.0009
Vacuole 0.006 0.001 0.0008 0.0005 0.0009 0.0012 0.002 0.0005 0.0007 0.0002 0.0003 0.0003 0.0016 0.0001 0.0003 0.0002 0.0006 0.0003
Vacuole Periphery 0.002 0.0001 0.0002 0.0001 0.0002 0.0003 0.0006 0.0003 0.0002 0 0.0001 0.0001 0.0004 0 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 201.8631 192.6874 137.3085 113.0787 159.6962 176.9599 173.5653 133.8426 102.55 167.6201
Translational Efficiency 1.5927 1.5987 1.3356 1.4594 1.9585 1.8274 1.7234 1.4084 1.718 1.8021

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mannose-6-phosphate isomerase; catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Pmi40

Pmi40


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmi40-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available