Standard name
Human Ortholog
Description Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0.12 0.14 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.07 0.12 0.12 0 0 0.05 0 0 0.06 0 0.1 0.06 0.12 0.07 0.06 0 0 0 0 0 0 0
Cytoplasm 0.29 0.53 0.44 0.58 0.52 0.71 0.51 0.56 0.7 0.6 0.8 0.52 0.53 0.65 0.7 0.55 0.51 0.62 0.09 0.19 0.1 0.18 0.2 0.15
Endoplasmic Reticulum 0.72 0.51 0.52 0.26 0.43 0.16 0.39 0.23 0.12 0.16 0 0.08 0.51 0.4 0.31 0 0 0 0.84 0.69 0.78 0.7 0.68 0.71
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 0.05 0.07 0.09 0.09 0.19 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.08 0.11 0.08 0.11 0.1 0.16 0.1 0.13 0.17 0.16 0 0 0 0.1 0.13 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 4 7 4 6 4 6 16 4 14 3 6 3 7 5 18 14 6 3 2 4 3 5 8
Bud 3 1 2 3 1 6 0 4 1 13 1 6 0 2 0 2 6 5 0 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 1 3 3 6
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 11 10 11 25 27 1 8 16 5 11 7 5 32 23 59 10 6 3 1 1 3 8 8 10
Cytoplasm 71 104 75 123 118 88 122 174 128 151 93 79 166 237 347 84 53 47 23 44 20 48 62 55
Endoplasmic Reticulum 178 100 88 54 97 20 94 71 21 39 5 12 160 146 153 5 3 0 224 160 159 189 211 273
Endosome 0 0 1 4 0 2 1 5 2 4 2 14 1 4 3 9 5 1 1 2 1 1 1 5
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 13 6 5 2 0 1 2 0 1
Mitochondria 2 0 0 6 0 11 0 17 13 22 10 28 0 0 1 3 7 2 2 2 5 4 3 2
Nucleus 0 0 0 1 1 1 5 3 7 4 4 2 0 0 0 4 3 3 0 1 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 8 4 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 6 0 0 0 0 0 0 0
Vac/Vac Membrane 5 0 13 23 19 14 24 50 19 32 20 24 2 1 9 16 13 12 1 8 4 4 9 10
Unique Cell Count 248 197 169 211 226 124 238 311 182 251 116 151 315 367 496 153 103 76 266 233 204 271 310 385
Labelled Cell Count 277 219 197 243 270 148 260 356 200 290 145 178 364 420 579 182 126 85 266 233 204 271 310 385


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 10.2 9.8 9.7 11.6 8.5 9.7 9.1 9.0 8.8 10.4 8.6 11.0 10.7 11.6 14.4 13.5 13.8 9.4 9.2 9.7
Std Deviation (1e-4) 1.3 1.5 1.7 1.4 1.6 6.1 1.5 1.5 2.3 1.5 7.6 1.5 1.9 1.8 2.0 3.3 2.9 3.9 2.1 1.9 2.0
Intensity Change (Log2) -0.01 0.24 -0.21 -0.02 -0.11 -0.12 -0.15 0.09 -0.2 0.16 0.13 0.24 0.56 0.47 0.49 -0.06 -0.09 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -0.8 0 -0.9 0.5 -1.0 0.7 0 -0.1 0 0 0 2.6 2.8 0
Bud 0 0 0 0 0 0 2.2 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.8 1.8 -2.4 -1.5 -0.6 -1.7 -1.0 -0.2 -1.3 1.3 -0.1 2.0 0 -0.2 0
Cytoplasm 2.7 1.5 4.5 1.4 2.4 4.9 3.2 6.0 1.4 1.7 4.4 6.0 1.9 1.1 2.5
Endoplasmic Reticulum -5.3 -1.8 -6.3 -2.5 -6.5 -8.2 -8.0 -8.4 -8.5 -0.3 -2.7 -5.0 -9.6 -8.3 -7.9
Endosome 0 0 0 0 0 0 0 0 3.7 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.9 0 0
Mitochondria 0 0 0 0 3.1 3.5 4.0 0 5.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.1 0.3 1.1 0.8 2.6 0.9 1.6 2.5 2.3 -4.3 0 -3.7 0.9 1.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5137 7.9039 7.6953 7.1266 6.9707 7.6304 10.4407 11.5339 10.8255 10.6208 9.7925 10.6195 10.6632 12.3295 10.8503 9.8948 10.6253 11.0541
Actin 0.0058 0.0012 0.0041 0.0022 0.0075 0.002 0.0005 0.0027 0.0011 0.0007 0.0011 0.0006 0.011 0.0019 0.0155 0.0101 0.0305 0.0013
Bud 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0007 0.0002 0.0003 0.0001 0.0004 0.0001 0.0001 0.0002 0.0004 0.0002 0
Bud Neck 0.0011 0.0004 0.0015 0.0006 0.0031 0.0045 0.0004 0.0027 0.0013 0.0052 0.0024 0.0056 0.0008 0.0005 0.002 0.0013 0.002 0.0068
Bud Periphery 0.0002 0.0001 0.0004 0.0001 0.0004 0.0002 0.0002 0.0005 0.0003 0.0019 0.0003 0.0007 0.0002 0.0002 0.0003 0.0008 0.0003 0.0001
Bud Site 0.0004 0.0017 0.0007 0.0001 0.0003 0.0001 0.0026 0.0172 0.002 0.0004 0.0003 0.0003 0.0007 0.0032 0.0097 0.0022 0.0007 0.0002
Cell Periphery 0.0027 0.0071 0.0057 0.0024 0.007 0.0023 0.0047 0.0077 0.0054 0.0088 0.0058 0.0028 0.0043 0.0065 0.0068 0.0032 0.0036 0.002
Cytoplasm 0.1199 0.2155 0.1716 0.112 0.3023 0.2249 0.1548 0.192 0.2436 0.1524 0.2941 0.1744 0.1475 0.3001 0.1868 0.1549 0.2717 0.1843
Cytoplasmic Foci 0.0053 0.0069 0.0071 0.0052 0.0136 0.01 0.0054 0.0103 0.0073 0.01 0.0123 0.0082 0.0134 0.0062 0.0102 0.0113 0.02 0.0085
Eisosomes 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0 0.0002 0 0 0.0023 0.0001 0.0001 0.0002 0.0003 0
Endoplasmic Reticulum 0.7864 0.6883 0.7425 0.8223 0.5661 0.6795 0.7631 0.6905 0.6649 0.7408 0.5921 0.754 0.7344 0.6282 0.6799 0.725 0.5109 0.7571
Endosome 0.0193 0.0218 0.0158 0.0075 0.0198 0.022 0.0175 0.0176 0.0194 0.0177 0.0204 0.0163 0.0185 0.0106 0.0146 0.0274 0.0491 0.01
Golgi 0.0024 0.0039 0.004 0.0037 0.0082 0.0051 0.0031 0.0035 0.0032 0.0016 0.0054 0.0024 0.0099 0.0031 0.0035 0.0121 0.0111 0.0022
Lipid Particles 0.0313 0.031 0.0319 0.0352 0.0553 0.0385 0.0207 0.0246 0.0246 0.0396 0.0535 0.0155 0.0207 0.0261 0.0527 0.0269 0.049 0.014
Mitochondria 0.0011 0.0002 0.0004 0.0002 0.0023 0.0007 0.0005 0.0003 0.0004 0.0003 0.0006 0.0016 0.0077 0.0011 0.0005 0.0032 0.0044 0.0002
None 0.0049 0.0022 0.0007 0.0002 0.0016 0.0007 0.0032 0.0022 0.0018 0.0044 0.0011 0.0017 0.0044 0.002 0.0011 0.0028 0.007 0.0017
Nuclear Periphery 0.0088 0.007 0.0057 0.0037 0.0043 0.0036 0.0105 0.0057 0.0055 0.005 0.0026 0.0058 0.0105 0.0035 0.005 0.0067 0.0043 0.0036
Nucleolus 0.0001 0.0001 0 0 0 0 0.0003 0.0019 0.0004 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002
Nucleus 0.0009 0.0015 0.0008 0.0004 0.0014 0.0008 0.0015 0.0028 0.0027 0.0013 0.0009 0.0017 0.001 0.0007 0.0007 0.0013 0.0009 0.0012
Peroxisomes 0.0014 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0011 0.0001 0.0038 0.0014 0.0248 0.0002
Punctate Nuclear 0.0005 0.0004 0.0003 0.0001 0.0006 0.0004 0.0005 0.0008 0.002 0.001 0.0005 0.0007 0.002 0.0003 0.0011 0.0014 0.0027 0.002
Vacuole 0.0066 0.0102 0.0061 0.0036 0.0051 0.0042 0.0099 0.0155 0.0134 0.0077 0.0058 0.0066 0.009 0.0053 0.005 0.0059 0.0054 0.0043
Vacuole Periphery 0.0005 0.0004 0.0004 0.0002 0.0004 0.0004 0.0006 0.0004 0.0005 0.0004 0.0003 0.0005 0.0004 0.0003 0.0003 0.0012 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.3166 66.6087 106.2145 115.5106 71.9417 98.2374 111.7691 120.892 160.0333 92.9666
Translational Efficiency 1.1027 1.2971 1.3734 1.5263 1.2435 1.0552 1.2921 1.5832 1.4557 1.3417

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation
Localization
Cell Percentages ER (92%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Fmp52

Fmp52


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp52-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available