Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 4 | 7 | 4 | 6 | 4 | 6 | 16 | 4 | 14 | 3 | 6 | 3 | 7 | 5 | 18 | 14 | 6 | 3 | 2 | 4 | 3 | 5 | 8 |
Bud | 3 | 1 | 2 | 3 | 1 | 6 | 0 | 4 | 1 | 13 | 1 | 6 | 0 | 2 | 0 | 2 | 6 | 5 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 3 | 3 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 11 | 10 | 11 | 25 | 27 | 1 | 8 | 16 | 5 | 11 | 7 | 5 | 32 | 23 | 59 | 10 | 6 | 3 | 1 | 1 | 3 | 8 | 8 | 10 |
Cytoplasm | 71 | 104 | 75 | 123 | 118 | 88 | 122 | 174 | 128 | 151 | 93 | 79 | 166 | 237 | 347 | 84 | 53 | 47 | 23 | 44 | 20 | 48 | 62 | 55 |
Endoplasmic Reticulum | 178 | 100 | 88 | 54 | 97 | 20 | 94 | 71 | 21 | 39 | 5 | 12 | 160 | 146 | 153 | 5 | 3 | 0 | 224 | 160 | 159 | 189 | 211 | 273 |
Endosome | 0 | 0 | 1 | 4 | 0 | 2 | 1 | 5 | 2 | 4 | 2 | 14 | 1 | 4 | 3 | 9 | 5 | 1 | 1 | 2 | 1 | 1 | 1 | 5 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 13 | 6 | 5 | 2 | 0 | 1 | 2 | 0 | 1 |
Mitochondria | 2 | 0 | 0 | 6 | 0 | 11 | 0 | 17 | 13 | 22 | 10 | 28 | 0 | 0 | 1 | 3 | 7 | 2 | 2 | 2 | 5 | 4 | 3 | 2 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 3 | 7 | 4 | 4 | 2 | 0 | 0 | 0 | 4 | 3 | 3 | 0 | 1 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 8 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 5 | 0 | 13 | 23 | 19 | 14 | 24 | 50 | 19 | 32 | 20 | 24 | 2 | 1 | 9 | 16 | 13 | 12 | 1 | 8 | 4 | 4 | 9 | 10 |
Unique Cell Count | 248 | 197 | 169 | 211 | 226 | 124 | 238 | 311 | 182 | 251 | 116 | 151 | 315 | 367 | 496 | 153 | 103 | 76 | 266 | 233 | 204 | 271 | 310 | 385 |
Labelled Cell Count | 277 | 219 | 197 | 243 | 270 | 148 | 260 | 356 | 200 | 290 | 145 | 178 | 364 | 420 | 579 | 182 | 126 | 85 | 266 | 233 | 204 | 271 | 310 | 385 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 10.2 | 9.8 | 9.7 | 11.6 | 8.5 | 9.7 | 9.1 | 9.0 | 8.8 | 10.4 | 8.6 | 11.0 | 10.7 | 11.6 | 14.4 | 13.5 | 13.8 | 9.4 | 9.2 | 9.7 |
Std Deviation (1e-4) | 1.3 | 1.5 | 1.7 | 1.4 | 1.6 | 6.1 | 1.5 | 1.5 | 2.3 | 1.5 | 7.6 | 1.5 | 1.9 | 1.8 | 2.0 | 3.3 | 2.9 | 3.9 | 2.1 | 1.9 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.24 | -0.21 | -0.02 | -0.11 | -0.12 | -0.15 | 0.09 | -0.2 | 0.16 | 0.13 | 0.24 | 0.56 | 0.47 | 0.49 | -0.06 | -0.09 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -0.8 | 0 | -0.9 | 0.5 | -1.0 | 0.7 | 0 | -0.1 | 0 | 0 | 0 | 2.6 | 2.8 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.8 | 1.8 | -2.4 | -1.5 | -0.6 | -1.7 | -1.0 | -0.2 | -1.3 | 1.3 | -0.1 | 2.0 | 0 | -0.2 | 0 |
Cytoplasm | 2.7 | 1.5 | 4.5 | 1.4 | 2.4 | 4.9 | 3.2 | 6.0 | 1.4 | 1.7 | 4.4 | 6.0 | 1.9 | 1.1 | 2.5 |
Endoplasmic Reticulum | -5.3 | -1.8 | -6.3 | -2.5 | -6.5 | -8.2 | -8.0 | -8.4 | -8.5 | -0.3 | -2.7 | -5.0 | -9.6 | -8.3 | -7.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3.1 | 3.5 | 4.0 | 0 | 5.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.1 | 0.3 | 1.1 | 0.8 | 2.6 | 0.9 | 1.6 | 2.5 | 2.3 | -4.3 | 0 | -3.7 | 0.9 | 1.3 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.5137 | 7.9039 | 7.6953 | 7.1266 | 6.9707 | 7.6304 | 10.4407 | 11.5339 | 10.8255 | 10.6208 | 9.7925 | 10.6195 | 10.6632 | 12.3295 | 10.8503 | 9.8948 | 10.6253 | 11.0541 |
Actin | 0.0058 | 0.0012 | 0.0041 | 0.0022 | 0.0075 | 0.002 | 0.0005 | 0.0027 | 0.0011 | 0.0007 | 0.0011 | 0.0006 | 0.011 | 0.0019 | 0.0155 | 0.0101 | 0.0305 | 0.0013 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0 |
Bud Neck | 0.0011 | 0.0004 | 0.0015 | 0.0006 | 0.0031 | 0.0045 | 0.0004 | 0.0027 | 0.0013 | 0.0052 | 0.0024 | 0.0056 | 0.0008 | 0.0005 | 0.002 | 0.0013 | 0.002 | 0.0068 |
Bud Periphery | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0019 | 0.0003 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0008 | 0.0003 | 0.0001 |
Bud Site | 0.0004 | 0.0017 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0026 | 0.0172 | 0.002 | 0.0004 | 0.0003 | 0.0003 | 0.0007 | 0.0032 | 0.0097 | 0.0022 | 0.0007 | 0.0002 |
Cell Periphery | 0.0027 | 0.0071 | 0.0057 | 0.0024 | 0.007 | 0.0023 | 0.0047 | 0.0077 | 0.0054 | 0.0088 | 0.0058 | 0.0028 | 0.0043 | 0.0065 | 0.0068 | 0.0032 | 0.0036 | 0.002 |
Cytoplasm | 0.1199 | 0.2155 | 0.1716 | 0.112 | 0.3023 | 0.2249 | 0.1548 | 0.192 | 0.2436 | 0.1524 | 0.2941 | 0.1744 | 0.1475 | 0.3001 | 0.1868 | 0.1549 | 0.2717 | 0.1843 |
Cytoplasmic Foci | 0.0053 | 0.0069 | 0.0071 | 0.0052 | 0.0136 | 0.01 | 0.0054 | 0.0103 | 0.0073 | 0.01 | 0.0123 | 0.0082 | 0.0134 | 0.0062 | 0.0102 | 0.0113 | 0.02 | 0.0085 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0023 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.7864 | 0.6883 | 0.7425 | 0.8223 | 0.5661 | 0.6795 | 0.7631 | 0.6905 | 0.6649 | 0.7408 | 0.5921 | 0.754 | 0.7344 | 0.6282 | 0.6799 | 0.725 | 0.5109 | 0.7571 |
Endosome | 0.0193 | 0.0218 | 0.0158 | 0.0075 | 0.0198 | 0.022 | 0.0175 | 0.0176 | 0.0194 | 0.0177 | 0.0204 | 0.0163 | 0.0185 | 0.0106 | 0.0146 | 0.0274 | 0.0491 | 0.01 |
Golgi | 0.0024 | 0.0039 | 0.004 | 0.0037 | 0.0082 | 0.0051 | 0.0031 | 0.0035 | 0.0032 | 0.0016 | 0.0054 | 0.0024 | 0.0099 | 0.0031 | 0.0035 | 0.0121 | 0.0111 | 0.0022 |
Lipid Particles | 0.0313 | 0.031 | 0.0319 | 0.0352 | 0.0553 | 0.0385 | 0.0207 | 0.0246 | 0.0246 | 0.0396 | 0.0535 | 0.0155 | 0.0207 | 0.0261 | 0.0527 | 0.0269 | 0.049 | 0.014 |
Mitochondria | 0.0011 | 0.0002 | 0.0004 | 0.0002 | 0.0023 | 0.0007 | 0.0005 | 0.0003 | 0.0004 | 0.0003 | 0.0006 | 0.0016 | 0.0077 | 0.0011 | 0.0005 | 0.0032 | 0.0044 | 0.0002 |
None | 0.0049 | 0.0022 | 0.0007 | 0.0002 | 0.0016 | 0.0007 | 0.0032 | 0.0022 | 0.0018 | 0.0044 | 0.0011 | 0.0017 | 0.0044 | 0.002 | 0.0011 | 0.0028 | 0.007 | 0.0017 |
Nuclear Periphery | 0.0088 | 0.007 | 0.0057 | 0.0037 | 0.0043 | 0.0036 | 0.0105 | 0.0057 | 0.0055 | 0.005 | 0.0026 | 0.0058 | 0.0105 | 0.0035 | 0.005 | 0.0067 | 0.0043 | 0.0036 |
Nucleolus | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 | 0.0019 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Nucleus | 0.0009 | 0.0015 | 0.0008 | 0.0004 | 0.0014 | 0.0008 | 0.0015 | 0.0028 | 0.0027 | 0.0013 | 0.0009 | 0.0017 | 0.001 | 0.0007 | 0.0007 | 0.0013 | 0.0009 | 0.0012 |
Peroxisomes | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0038 | 0.0014 | 0.0248 | 0.0002 |
Punctate Nuclear | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0004 | 0.0005 | 0.0008 | 0.002 | 0.001 | 0.0005 | 0.0007 | 0.002 | 0.0003 | 0.0011 | 0.0014 | 0.0027 | 0.002 |
Vacuole | 0.0066 | 0.0102 | 0.0061 | 0.0036 | 0.0051 | 0.0042 | 0.0099 | 0.0155 | 0.0134 | 0.0077 | 0.0058 | 0.0066 | 0.009 | 0.0053 | 0.005 | 0.0059 | 0.0054 | 0.0043 |
Vacuole Periphery | 0.0005 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0004 | 0.0006 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0012 | 0.0009 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 52.3166 | 66.6087 | 106.2145 | 115.5106 | 71.9417 | 98.2374 | 111.7691 | 120.892 | 160.0333 | 92.9666 |
Translational Efficiency | 1.1027 | 1.2971 | 1.3734 | 1.5263 | 1.2435 | 1.0552 | 1.2921 | 1.5832 | 1.4557 | 1.3417 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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