Standard name
Human Ortholog
Description Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.6 0.64 0.76 0.76 0.8 0.78 0.78 0.79 0.8 0.78 0.77 0.82 0.89 0.9 0.8 0.72 0.07 0 0 0 0.08 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.06 0.07 0 0 0 0 0 0
Endosome 0 0.07 0.17 0.14 0.1 0 0 0 0 0 0 0 0 0 0.05 0.06 0.12 0.16 0.23 0.26 0.24 0.11 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.1 0 0.06 0.08 0.32 0.34 0.3 0.41 0.4 0.43 0.33 0 0 0 0.05 0 0.12 0.1 0.11 0.26 0.18 0.16
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.31 0.27 0.13 0.14 0 0 0 0 0 0 0 0 0 0 0.18 0.29 0.55 0.56 0.5 0.38 0.52 0.46
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 3 0 0 0 0 1 0 0 0 0 14 3 5 2 0 0 3 3 0 1 2
Bud 0 4 0 3 4 4 13 13 21 20 17 18 3 1 2 1 0 1 2 3 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 4 1 2 1 0 0 1 1 0 1 0
Bud Site 0 0 0 0 0 0 0 2 1 0 3 0 0 0 0 0 0
Cell Periphery 0 0 0 1 1 0 1 0 0 0 0 1 5 0 3 0 0 0 0 0 0 0 0
Cytoplasm 27 228 232 179 243 283 345 332 284 372 283 364 204 360 335 348 157 6 16 9 3 15 17
Endoplasmic Reticulum 1 17 7 0 0 1 0 0 0 0 0 0 17 3 5 25 16 0 3 9 0 5 6
Endosome 0 26 62 34 31 3 8 7 3 5 1 3 9 18 20 28 26 14 102 100 20 21 22
Golgi 0 0 3 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 15 12 2 4 6
Mitochondria 9 38 11 13 27 115 150 127 149 186 155 155 0 0 1 20 5 10 46 41 23 34 27
Nucleus 1 2 0 0 0 1 1 1 3 1 1 0 1 1 1 1 0 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 1 1 1 0 2 0 2 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 1 0 0 0 1 1 0 1 2 1 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 1 1 2 0 1 2 0 2 0 2 0 1 0 1 0 0 1 1 0 0 1
SpindlePole 0 2 7 5 2 3 9 5 9 9 9 19 0 7 4 5 1 2 1 2 0 2 1
Vac/Vac Membrane 15 119 98 31 44 13 12 7 4 12 4 8 7 14 8 78 63 49 250 195 33 100 79
Unique Cell Count 46 378 361 235 318 354 443 423 360 465 364 470 250 404 372 433 217 89 450 391 88 193 172
Labelled Cell Count 54 442 425 267 355 424 542 498 476 609 477 571 264 409 387 511 268 89 450 391 88 193 172


Ambiguous, Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.5 5.2 4.8 4.7 3.5 3.3 3.7 3.1 3.1 3.1 3.0 5.8 5.6 5.7 4.7 5.4 5.9 5.7 6.6 6.9
Std Deviation (1e-4) 0.7 0.8 1.4 1.5 2.0 1.2 1.1 1.3 0.8 1.4 1.1 0.9 0.7 0.8 0.8 0.8 0.9 1.6 0.8 1.5 2.4
Intensity Change (Log2) -0.12 -0.17 -0.56 -0.65 -0.49 -0.76 -0.76 -0.76 -0.81 0.16 0.09 0.13 -0.14 0.06 0.18 0.13 0.34 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.5 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.1 3.4 4.7 4.3 4.4 4.4 5.1 4.0 4.2 4.7 8.2 8.3 5.1 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 3.0 0 -0.6 2.7 3.2
Endosome 0 0 0 0 0 0 0 0 0 -5.1 -5.7 -5.1 -4.7 -1.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 -3.5 -3.0 1.1 -0.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 -0.2 -1.0 -0.9 0
Vacuole -4.0 -4.3 -8.7 -10.0 -10.4 -10.0 -10.3 -10.1 -10.9 -7.8 -9.2 -9.6 -3.1 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0685 1.3522 1.393 1.5778 1.492 1.2089 2.8244 3.7528 3.7512 3.4004 3.1815 4.0331 3.045 3.5138 3.6417 3.5747 3.447 3.8494
Actin 0.0316 0.0009 0.0208 0.023 0.032 0.004 0.0018 0.0031 0.0012 0.0015 0.0003 0.0012 0.0002 0.0002 0.0011 0.0001 0.0001 0.0004
Bud 0.0036 0.0032 0.0007 0.0004 0.0017 0.0002 0.0007 0.0012 0.0012 0.0013 0.0004 0.0004 0.0006 0.0005 0.0003 0.0003 0.0002 0.0002
Bud Neck 0.0029 0.0006 0.0014 0.0018 0.0006 0.0012 0.0053 0.0004 0.0003 0.0005 0.0005 0.0008 0.0003 0.0002 0.0003 0.0074 0.0003 0.0007
Bud Periphery 0.0062 0.0016 0.0008 0.0005 0.0031 0.0004 0.0008 0.0015 0.002 0.0038 0.0005 0.0006 0.0004 0.0007 0.0003 0.0004 0.0002 0.0003
Bud Site 0.0046 0.0081 0.0015 0.0009 0.0036 0.0002 0.0069 0.0061 0.0014 0.0011 0.0005 0.0002 0.0018 0.0007 0.0012 0.0004 0.0003 0.0002
Cell Periphery 0.0004 0.0005 0.0002 0.0002 0.0003 0.0001 0.0004 0.0004 0.0003 0.0003 0.0003 0.0001 0.0003 0.0002 0.0002 0.0002 0.0002 0.0001
Cytoplasm 0.1396 0.247 0.1899 0.15 0.1229 0.2613 0.1924 0.1897 0.1879 0.0678 0.1233 0.18 0.2278 0.2521 0.2267 0.2022 0.2743 0.2338
Cytoplasmic Foci 0.1339 0.1152 0.1442 0.1625 0.1479 0.124 0.1489 0.1025 0.1289 0.1059 0.1694 0.1387 0.1253 0.1201 0.107 0.1635 0.1095 0.1547
Eisosomes 0.0003 0.0003 0.0002 0.0004 0.0004 0 0.0001 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.007 0.0043 0.0071 0.0061 0.0056 0.0045 0.0069 0.0043 0.004 0.0027 0.0038 0.0039 0.0049 0.0057 0.0104 0.0028 0.0064 0.0035
Endosome 0.2744 0.2954 0.352 0.4156 0.3644 0.3337 0.3214 0.2442 0.3032 0.5206 0.4077 0.3219 0.3476 0.357 0.3427 0.4634 0.3644 0.3628
Golgi 0.0191 0.0123 0.0183 0.015 0.0208 0.0082 0.0119 0.0096 0.0129 0.0212 0.014 0.0139 0.0202 0.0189 0.0123 0.0203 0.0222 0.0137
Lipid Particles 0.0236 0.0064 0.0091 0.0116 0.0139 0.007 0.0078 0.0095 0.0076 0.0054 0.0054 0.0113 0.0044 0.0057 0.0037 0.0041 0.0029 0.0065
Mitochondria 0.013 0.0077 0.0131 0.0105 0.019 0.0045 0.0032 0.0152 0.008 0.0392 0.0082 0.016 0.0114 0.0063 0.0066 0.0066 0.0064 0.0045
None 0.2583 0.2424 0.1922 0.0877 0.2296 0.1925 0.2392 0.3382 0.2845 0.1587 0.2012 0.2588 0.2189 0.1873 0.2375 0.0517 0.1658 0.184
Nuclear Periphery 0.0091 0.0018 0.0054 0.0151 0.0041 0.0047 0.0053 0.0027 0.0032 0.0035 0.0019 0.0067 0.0013 0.0031 0.0038 0.0012 0.0022 0.0011
Nucleolus 0.0008 0.0012 0.0007 0.0013 0.0005 0.0004 0.0012 0.0005 0.0009 0.0004 0.0005 0.0005 0.0004 0.0003 0.0003 0.0003 0.0002 0.0002
Nucleus 0.0122 0.0023 0.0024 0.0126 0.0022 0.0037 0.0036 0.0016 0.0019 0.001 0.0012 0.0025 0.0013 0.0017 0.0018 0.001 0.0013 0.0008
Peroxisomes 0.013 0.0051 0.004 0.005 0.0053 0.0024 0.0025 0.0041 0.0093 0.003 0.0242 0.0026 0.002 0.0018 0.0026 0.0033 0.0022 0.0035
Punctate Nuclear 0.0077 0.0016 0.0021 0.0083 0.003 0.0042 0.002 0.0011 0.0043 0.0005 0.0012 0.0014 0.0006 0.0007 0.0008 0.0017 0.0004 0.0011
Vacuole 0.0326 0.0359 0.0273 0.0602 0.0151 0.0336 0.0325 0.0543 0.0265 0.0418 0.0281 0.0288 0.0252 0.0301 0.0305 0.0538 0.0345 0.0212
Vacuole Periphery 0.006 0.0063 0.0067 0.0113 0.0042 0.0092 0.0052 0.0098 0.0103 0.0197 0.0073 0.0096 0.005 0.0065 0.0097 0.0154 0.0062 0.0068

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4465 16.153 6.9597 14.4795 10.5803 12.2338 12.6444 14.0457 13.1786 16.5792
Translational Efficiency 0.3904 0.375 0.7632 0.449 0.5157 0.4622 0.5614 0.4114 0.3864 0.3166

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity
Localization
Cell Percentages vacuole (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ynd1

Ynd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available