Standard name
Human Ortholog
Description GTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0.06 0.09 0.11 0.08 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.11 0.31 0.11 0.33 0.22 0.19 0.24 0.24 0.27 0.21 0.26 0.24 0.17 0.06 0.08 0.1 0 0 0 0 0.07 0.05
Nucleus 0.79 0.83 0.4 0.16 0.16 0.28 0.4 0.46 0.31 0.45 0.32 0.52 0.12 0.05 0 0.43 0.42 0.51 0.51 0.67 0.26 0.22 0.15 0.15
Nuclear Periphery 0 0 0 0 0 0.07 0.06 0.06 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.32 0.17 0.6 0.84 0.74 0.62 0.58 0.47 0.61 0.52 0.62 0.45 0.84 0.86 0.83 0.45 0.46 0.44 0.39 0.26 0.64 0.57 0.49 0.39
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.05 0.05 0.06 0.13 0.22 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 2 2 1 1 0 0 0 0 1 1 0 1 3 2
Bud Neck 0 0 0 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 3 5 3 1 2 9 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 3 2 4 4 6 4 6 5 3 7 1 2 18 35 47 38 39 17 1 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 5 2 0 0 0 0 0 0
Endosome 0 2 0 0 3 1 2 1 1 0 2 0 3 1 6 3 1 0 2 0 0 2 2 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 2
Mitochondria 20 6 15 23 13 25 42 28 22 38 40 50 78 96 69 32 34 26 0 0 1 6 18 14
Nucleus 204 251 57 12 20 21 75 70 29 72 46 124 37 20 17 214 171 131 146 226 40 48 41 39
Nuclear Periphery 1 1 1 0 2 5 11 9 3 5 9 9 0 1 0 3 0 0 3 3 2 1 4 6
Nucleolus 82 53 84 63 91 47 110 71 57 83 91 106 254 336 345 221 191 114 111 88 97 125 134 104
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 1 8 11 1 0 1 0 0 0 0 0 0
SpindlePole 0 0 1 2 2 0 3 2 2 1 1 1 1 9 20 1 1 0 1 0 0 2 4 4
Vac/Vac Membrane 2 8 4 0 4 0 1 2 1 0 1 2 1 4 4 33 29 11 15 18 8 28 61 81
Unique Cell Count 257 304 141 75 123 76 189 151 93 160 146 237 304 392 418 494 412 257 287 340 153 220 277 271
Labelled Cell Count 312 323 166 104 142 103 253 194 122 207 196 305 394 513 522 554 472 303 287 340 153 220 277 271


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.2 29.7 22.5 18.1 16.0 12.0 9.9 9.7 9.5 8.1 8.6 8.7 26.6 23.3 22.4 29.6 36.9 31.7 22.1 19.8 20.7
Std Deviation (1e-4) 8.3 8.2 6.6 6.3 5.9 5.0 3.2 3.1 2.7 2.4 2.5 2.5 10.7 9.7 10.2 13.7 16.8 14.3 6.5 5.7 6.1
Intensity Change (Log2) – – – -0.31 -0.49 -0.9 -1.18 -1.22 -1.24 -1.47 -1.39 -1.38 0.24 0.05 -0.0 0.4 0.72 0.5 -0.02 -0.18 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.7 0 0 1.4 2.4 3.0 2.0 2.5 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.7 0 4.0 2.8 1.9 2.7 3.0 3.6 2.6 3.6 3.5 1.7 -1.7 -0.9 -0.2
Nucleus -3.7 -4.3 -1.9 -0.1 1.0 -1.4 0.8 -1.6 2.2 -6.8 -10.2 -11.0 0.6 0.2 2.0
Nuclear Periphery 0 0 0 2.5 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.7 2.5 0.3 -0.3 -2.1 0.3 -1.3 0.5 -2.8 5.5 6.5 5.6 -3.1 -2.7 -2.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.7 1.8 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.4496 28.2283 26.7992 25.8131 24.0027 27.6566 22.5189 27.3524 25.1957 24.2928 22.2788 24.6331 22.2696 25.376 28.0285 20.8166 22.3745 24.6529
Actin 0.0138 0.0001 0.0005 0.0002 0.042 0.0002 0.006 0.0004 0.0063 0 0.0323 0.0001 0 0.0163 0 0.0421 0 0.0001
Bud 0.0011 0.0009 0.0002 0.0003 0.0004 0.0001 0.0002 0.0001 0.0001 0 0.0007 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0001
Bud Neck 0.002 0.0002 0.0001 0.0001 0.0005 0.0002 0.0004 0.0002 0.0029 0.0001 0.0006 0.0002 0.0001 0.0001 0.0001 0.001 0.0001 0.0001
Bud Periphery 0.0017 0.0008 0.0002 0.0004 0.0008 0.0004 0.0003 0.0001 0.0002 0.0002 0.0014 0.0004 0.0003 0.0002 0.0001 0.0005 0.0001 0.0004
Bud Site 0.0074 0.0013 0.0003 0.0006 0.0009 0.0001 0.0006 0.0005 0.0013 0.0001 0.0018 0.0001 0.0002 0.0003 0.0001 0.0019 0.0001 0.0001
Cell Periphery 0.0028 0.0011 0.0005 0.0007 0.0004 0.0008 0.0005 0.0001 0.0004 0.0004 0.0007 0.0009 0.0003 0.0003 0.0002 0.0003 0.0002 0.0005
Cytoplasm 0.0012 0.0001 0.0176 0.0009 0.0001 0.0012 0.0042 0.0004 0.0006 0.0001 0.0005 0 0 0 0 0.0106 0 0
Cytoplasmic Foci 0.0233 0.0023 0.002 0.0014 0.0027 0.0034 0.0167 0.0046 0.0005 0.0002 0.0193 0.0001 0.0067 0.0039 0 0.0082 0 0
Eisosomes 0.0004 0 0 0.0001 0.0002 0 0.0002 0 0 0 0.0003 0 0.0001 0.0001 0 0.0005 0 0
Endoplasmic Reticulum 0.0005 0 0.0007 0.0001 0.0002 0.0003 0.0022 0 0.0017 0 0.0009 0 0 0 0 0.0071 0 0
Endosome 0.0021 0.0002 0.009 0.0002 0.0045 0.0009 0.0088 0.0003 0.0046 0.0001 0.0043 0.0001 0.0001 0.0008 0 0.0231 0 0
Golgi 0.0011 0 0.0031 0.0001 0.0196 0.0001 0.0012 0.0005 0.0022 0 0.0034 0 0 0.0043 0 0.0048 0 0
Lipid Particles 0.0115 0.0007 0.0006 0.0016 0.0052 0.0015 0.0094 0.0002 0.0006 0.0007 0.0073 0.0009 0.0009 0.0304 0.0002 0.007 0 0.0005
Mitochondria 0.0112 0.0079 0.0072 0.011 0.0072 0.0031 0.0065 0.0008 0.0024 0.0013 0.0134 0.0035 0.0044 0.0154 0.001 0.0026 0.0015 0.0189
None 0.0039 0.0002 0.0003 0.0008 0.0001 0.0057 0.0044 0 0.0002 0.0001 0.0028 0.0001 0.0005 0.0001 0 0.0151 0 0
Nuclear Periphery 0.0049 0.0144 0.0011 0.0047 0.0328 0.0099 0.009 0.0032 0.0036 0.0168 0.0359 0.0134 0.0013 0.0017 0.0018 0.0478 0.0005 0.0053
Nucleolus 0.5709 0.4151 0.6703 0.5792 0.4701 0.5724 0.5796 0.6273 0.7121 0.6094 0.4715 0.668 0.779 0.7015 0.8157 0.3962 0.4459 0.7193
Nucleus 0.2907 0.5399 0.2832 0.3853 0.3983 0.3766 0.3293 0.3558 0.2521 0.3523 0.3827 0.2942 0.2001 0.2149 0.1758 0.4115 0.5504 0.2466
Peroxisomes 0.0011 0.0006 0.0004 0.0004 0.0017 0.0001 0.0011 0.0035 0.0001 0 0.0021 0 0.0002 0.002 0 0.0027 0 0
Punctate Nuclear 0.0037 0.0006 0.0004 0.0012 0.0005 0.0008 0.0146 0.0012 0.0004 0.0005 0.0032 0.0006 0.001 0.0017 0.0002 0.0108 0.0001 0.0006
Vacuole 0.0107 0.0036 0.0012 0.0025 0.0017 0.0046 0.0018 0.0002 0.0024 0.0033 0.0039 0.0028 0.0019 0.0011 0.0009 0.0016 0.0004 0.0015
Vacuole Periphery 0.0338 0.0099 0.0013 0.0085 0.01 0.0177 0.0031 0.0009 0.0054 0.0144 0.0111 0.0145 0.0026 0.0048 0.0037 0.0041 0.0006 0.0059

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 122.1299 126.8468 108.1745 76.9012 90.1569 83.9619 89.0126 68.0448 56.7627 88.1251
Translational Efficiency 1.8817 1.9787 1.5611 1.8485 1.8723 1.818 1.7279 1.6514 1.5312 1.7127

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description GTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus
Localization
Cell Percentages nucleus (68%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Nug1

Nug1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nug1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available