Standard name | |
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Human Ortholog | |
Description | DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 6 | 9 | 5 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 1 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 2 | 0 | 0 | 5 | 13 | 18 | 10 | 23 | 4 | 18 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 0 | 4 | 7 | 16 | 17 | 26 | 43 | 19 | 21 | 12 | 10 | 5 | 32 | 34 | 33 | 13 | 25 | 1 | 0 | 1 | 2 | 2 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 2 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6 | 13 | 60 | 88 | 103 | 186 | 365 | 355 | 316 | 406 | 167 | 321 | 2 | 1 | 20 | 15 | 14 | 11 | 2 | 0 | 0 | 1 | 1 | 3 |
Nucleus | 291 | 37 | 108 | 132 | 270 | 229 | 446 | 440 | 274 | 394 | 166 | 297 | 105 | 198 | 186 | 175 | 68 | 117 | 277 | 66 | 104 | 69 | 157 | 163 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 7 | 2 | 9 | 5 | 9 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 1 | 2 | 0 | 0 | 1 | 0 | 2 | 6 | 3 | 12 | 3 | 10 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 3 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 3 | 2 |
Vac/Vac Membrane | 7 | 0 | 3 | 2 | 7 | 4 | 11 | 4 | 3 | 3 | 1 | 1 | 2 | 2 | 3 | 17 | 5 | 21 | 15 | 1 | 12 | 4 | 24 | 35 |
Unique Cell Count | 312 | 43 | 121 | 152 | 314 | 297 | 571 | 597 | 396 | 536 | 225 | 393 | 110 | 231 | 223 | 222 | 86 | 162 | 317 | 71 | 126 | 82 | 201 | 221 |
Labelled Cell Count | 319 | 52 | 178 | 229 | 398 | 442 | 876 | 880 | 636 | 875 | 361 | 674 | 114 | 238 | 245 | 248 | 103 | 186 | 317 | 71 | 126 | 82 | 201 | 221 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 4.5 | 3.7 | 3.7 | 4.0 | 3.7 | 3.4 | 3.5 | 3.1 | 3.1 | 3.4 | 2.9 | 5.8 | 6.0 | 4.8 | 6.9 | 6.8 | 7.9 | 5.8 | 5.3 | 5.8 |
Std Deviation (1e-4) | 0.9 | 1.0 | 0.4 | 1.0 | 1.0 | 1.1 | 1.0 | 1.1 | 1.0 | 1.2 | 1.5 | 0.8 | 1.0 | 1.8 | 1.2 | 1.8 | 1.5 | 2.1 | 2.8 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.12 | -0.01 | -0.11 | -0.06 | -0.25 | -0.26 | -0.1 | -0.36 | 0.66 | 0.69 | 0.39 | 0.9 | 0.88 | 1.09 | 0.66 | 0.53 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.0 | – | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2882 | 1.7992 | 1.5137 | 1.2955 | 1.2152 | 1.769 | 2.9937 | 2.7578 | 2.5902 | 2.0913 | 2.5721 | 2.6895 | 1.0248 | 1.0018 | 0.5561 | 0.67 | 0.1096 | 0.5534 |
Actin | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0212 | 0.0109 | 0.0036 | 0.0029 | 0.0045 | 0.0124 | 0.0196 | 0 | 0.0046 | 0.0024 | 0 | 0.001 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0015 | 0.0001 | 0.0068 | 0.0002 | 0.0008 | 0.0018 | 0.0006 | 0 | 0.0003 | 0.0003 | 0 | 0.0001 |
Bud Neck | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0011 | 0.0021 | 0.0001 | 0.0002 | 0.0008 | 0.0006 | 0.0026 | 0.0035 | 0 | 0.0007 | 0.0018 | 0.0002 | 0.0039 |
Bud Periphery | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0036 | 0.0001 | 0.0041 | 0.0004 | 0.0053 | 0.003 | 0.0013 | 0 | 0.001 | 0.0012 | 0 | 0.0002 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0.0066 | 0.0021 | 0.0012 | 0.0008 | 0.0017 | 0.0012 | 0.0059 | 0 | 0.0053 | 0.0011 | 0 | 0.005 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0 | 0.0015 | 0.0004 | 0 | 0.0001 |
Cytoplasm | 0.001 | 0.002 | 0.0016 | 0.0004 | 0.003 | 0.0002 | 0.0081 | 0.0009 | 0.0006 | 0.0003 | 0.0002 | 0.0015 | 0.0056 | 0.0006 | 0.0087 | 0.0036 | 0.0003 | 0.0002 |
Cytoplasmic Foci | 0 | 0 | 0 | 0 | 0 | 0 | 0.0201 | 0.0042 | 0.0037 | 0.002 | 0.0099 | 0.0011 | 0.0354 | 0 | 0.0075 | 0.0017 | 0 | 0.0009 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0006 | 0 | 0.0004 | 0.0005 | 0 | 0 |
Endoplasmic Reticulum | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.009 | 0.0001 | 0.0001 | 0.0004 | 0.0006 | 0.0028 | 0.0023 | 0.0001 | 0.0061 | 0.0014 | 0 | 0 |
Endosome | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0441 | 0.0017 | 0.0032 | 0.028 | 0.0089 | 0.006 | 0.0173 | 0 | 0.0038 | 0.0062 | 0 | 0.0004 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0.0204 | 0.0027 | 0.0044 | 0.0167 | 0.0108 | 0.0023 | 0.0052 | 0 | 0.0012 | 0.0009 | 0 | 0.0005 |
Lipid Particles | 0 | 0 | 0 | 0 | 0 | 0 | 0.0189 | 0.0038 | 0.001 | 0.0043 | 0.0123 | 0.0004 | 0.0195 | 0 | 0.0043 | 0.0025 | 0 | 0.0036 |
Mitochondria | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0208 | 0.0019 | 0.0031 | 0.0266 | 0.0376 | 0.0042 | 0.0164 | 0.0001 | 0.0036 | 0.012 | 0.0001 | 0.0005 |
None | 0.0147 | 0.0004 | 0.0027 | 0.0001 | 0.0043 | 0.0001 | 0.0046 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.017 | 0.0001 | 0.0081 | 0.0171 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.005 | 0.0135 | 0.0119 | 0.0032 | 0.009 | 0.0016 | 0.0521 | 0.0019 | 0.0016 | 0.0012 | 0.005 | 0.0014 | 0.0263 | 0.0135 | 0.0071 | 0.009 | 0.0011 | 0.0012 |
Nucleolus | 0.0059 | 0.0041 | 0.0044 | 0.0032 | 0.0126 | 0.0152 | 0.005 | 0.0033 | 0.0029 | 0.0024 | 0.0067 | 0.0123 | 0.0054 | 0.0031 | 0.0033 | 0.0059 | 0.0075 | 0.0149 |
Nucleus | 0.9662 | 0.9767 | 0.9759 | 0.9921 | 0.955 | 0.9794 | 0.7278 | 0.9593 | 0.957 | 0.9059 | 0.8798 | 0.9421 | 0.7593 | 0.9818 | 0.9143 | 0.9126 | 0.9895 | 0.9527 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0085 | 0.0026 | 0.0055 | 0.0007 | 0.0078 | 0.0004 | 0.0142 | 0 | 0.0042 | 0.0006 | 0 | 0.0053 |
Punctate Nuclear | 0.0062 | 0.0023 | 0.0028 | 0.0008 | 0.0146 | 0.0018 | 0.0077 | 0.0037 | 0.0003 | 0.0002 | 0.0007 | 0.0006 | 0.0402 | 0.0004 | 0.0122 | 0.0115 | 0.0009 | 0.0087 |
Vacuole | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0109 | 0.0001 | 0.0003 | 0.0037 | 0.0026 | 0.0028 | 0.0023 | 0.0001 | 0.0015 | 0.0049 | 0 | 0.0005 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.006 | 0.0002 | 0.0002 | 0.0022 | 0.0036 | 0.0008 | 0.0016 | 0.0001 | 0.0004 | 0.0022 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.0236 | 9.7547 | 9.5803 | 12.932 | 10.611 | 7.0569 | 11.4585 | 12.5306 | 11.0377 | 12.4389 |
Translational Efficiency | 1.0066 | 1.0312 | 0.9668 | 0.809 | 0.8974 | 1.0989 | 0.9505 | 0.7159 | 0.8184 | 0.7002 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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