Standard name
Human Ortholog
Description Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.05 0.12 0.09 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0.14 0.05 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05
Golgi 0.07 0 0 0 0 0 0 0 – 0 0 0 0.14 0.37 0.52 0.09 0.1 0.11 0 0.09 0 0.06 0.06 0.09
Mitochondria 0.87 0.94 0.93 0.92 0.82 0.9 0.89 0.88 – 0.93 0.93 0.94 0.86 0.7 0.61 0.11 0.1 0.05 0.84 0.72 0.76 0.75 0.69 0.65
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0.07 0.08 0.06 0.09 0.09 0.15 0.13 – 0.14 0.09 0.11 0.06 0 0 0.1 0.21 0.15 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.07 0.09 0.11 0.13 – 0.1 0.07 0.07 0 0 0 0 0.05 0.12 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0.21 0.12 0.16 0 0 0.12 0.07 0.09 0.09
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.09 0 0 0.07 0 0 0 – 0 0 0 0.12 0.2 0.18 0.16 0.14 0.15 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 1 3 1 0 0 0 – 0 1 1 1 9 5 1 1 2 4 3 3 3 15 12
Bud 1 2 8 5 1 6 5 19 – 11 13 9 1 1 0 8 2 3 0 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 1 0 0 4 5 6 – 7 4 1 0 0 0 1 3 1 – – – – – –
Cell Periphery 11 4 8 3 3 0 0 4 – 2 2 2 1 7 9 16 5 8 0 1 0 1 0 1
Cytoplasm 4 0 9 13 13 16 9 9 – 4 6 4 4 3 6 19 3 10 0 0 0 0 0 0
Endoplasmic Reticulum 2 3 3 4 4 7 5 7 – 6 1 7 1 5 7 5 2 2 0 0 1 0 0 1
Endosome 9 1 5 2 17 3 3 7 – 2 0 1 3 3 3 6 1 4 8 7 8 9 9 15
Golgi 27 9 4 2 2 0 0 0 – 0 0 0 15 54 100 12 6 8 18 22 13 13 17 27
Mitochondria 347 217 341 267 222 375 399 539 – 472 291 389 93 102 118 15 6 4 355 170 284 165 204 210
Nucleus 0 2 0 2 0 2 2 4 – 1 0 0 0 0 1 8 5 2 0 0 0 0 0 0
Nuclear Periphery 30 16 28 18 25 36 67 77 – 70 27 47 7 5 6 13 12 11 6 3 3 1 5 5
Nucleolus 16 8 13 7 20 38 48 78 – 52 22 31 1 0 0 5 3 9 1 1 1 1 1 2
Peroxisomes 13 3 28 5 7 2 9 9 – 6 4 6 4 3 5 28 7 12 18 6 46 16 26 29
SpindlePole 1 0 4 0 3 3 9 8 – 3 4 3 0 1 1 6 6 1 2 0 3 1 0 0
Vac/Vac Membrane 55 20 11 5 20 16 13 13 – 17 8 10 13 29 34 21 8 11 4 15 0 2 9 7
Unique Cell Count 398 232 368 290 272 415 449 610 509 312 415 108 145 193 134 58 74 423 238 373 222 298 322
Labelled Cell Count 516 288 464 336 338 508 574 780 653 383 511 144 222 295 164 70 88 423 238 373 222 298 322


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 8.9 7.9 8.5 8.7 7.6 7.4 7.7 – 7.5 7.3 7.7 9.6 9.9 10.2 7.2 7.6 9.8 9.7 10.4 10.9
Std Deviation (1e-4) 1.8 1.9 2.0 2.0 2.3 2.4 2.2 2.4 – 2.5 2.5 2.5 2.0 1.8 1.8 1.6 2.0 2.6 2.2 2.5 2.3
Intensity Change (Log2) – – – 0.1 0.14 -0.06 -0.09 -0.03 – -0.07 -0.11 -0.04 0.28 0.34 0.38 -0.14 -0.04 0.32 0.3 0.4 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud -0.4 0 -0.8 -1.2 0.9 – 0 1.5 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 1.6 4.5 0 0
Cytoplasm 1.4 1.6 1.1 -0.4 -1.1 – -2.0 -0.5 -1.6 0 0 0.5 5.1 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 3.4 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 11.6 14.7 0 0 0
Mitochondria -0.3 -4.2 -1.1 -1.8 -2.2 – 0 0.3 0.6 -2.1 -6.6 -9.2 -17.8 -15.0 -16.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.7 0.7 0.5 3.2 2.5 – 2.8 0.5 1.8 -0.4 -1.7 -2.1 0.8 3.2 2.0
Nucleolus -0.8 2.2 3.2 3.9 4.8 – 3.7 2.1 2.4 0 0 0 0 0 0
Peroxisomes -3.4 -2.8 -5.2 -3.8 -4.9 – -4.9 -3.9 -4.2 -1.4 -2.4 -2.4 4.2 0 2.4
SpindlePole 0 0 0 1.0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.0 2.5 0.7 -0.1 -0.8 – 0.3 -0.3 -0.5 3.8 6.5 6.1 5.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0371 0.0211 0.0363 0.0548 0.0014 0.0009 0.0115 0.0043 0.004 0.0001 0.0241 0.0017 0.0042 0.0071 0.0239 0.0007 0.0001 0.0015
Bud 0.0008 0.0024 0.0005 0.0019 0.0008 0 0.0003 0.0013 0.0001 0 0.0003 0 0.0008 0.0002 0.0003 0.0001 0 0
Bud Neck 0.0007 0.0005 0.0003 0.0003 0.0001 0 0.0003 0.0003 0 0 0.0002 0 0.0003 0.0001 0.0001 0 0 0
Bud Periphery 0.0019 0.0029 0.0009 0.004 0.0044 0.0001 0.0007 0.0013 0.0003 0.0001 0.0008 0.0001 0.0011 0.0003 0.0006 0.0002 0 0.0001
Bud Site 0.0046 0.0159 0.001 0.0032 0.0001 0 0.0011 0.0089 0.0001 0 0.0006 0 0.0021 0.0014 0.002 0.0001 0 0
Cell Periphery 0.001 0.0011 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0.0003 0 0.0001 0 0.0002 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.001 0.0004 0.0006 0.0001 0 0 0.002 0.0001 0.0006 0 0.0003 0 0.0039 0.0001 0 0 0 0
Cytoplasmic Foci 0.0209 0.0043 0.0035 0.0066 0.0021 0.0025 0.0062 0.0047 0.0008 0.0001 0.0037 0.0008 0.0258 0.0186 0.002 0.0019 0.0002 0.0014
Eisosomes 0.0012 0.0002 0.0003 0.0002 0.0001 0.0001 0.0004 0.0001 0.0007 0 0.0004 0.0001 0.0001 0.0005 0.0004 0 0 0.0003
Endoplasmic Reticulum 0.003 0.0003 0.0003 0.0001 0.0001 0.0001 0.0033 0.0001 0.0011 0 0.0016 0 0.0068 0 0 0 0 0.0001
Endosome 0.0268 0.0098 0.0047 0.0063 0.0083 0.0039 0.0135 0.0132 0.0022 0.0004 0.0148 0.0026 0.1982 0.0195 0.0006 0.0019 0.0001 0.0078
Golgi 0.0309 0.0119 0.0121 0.0162 0.0436 0.0102 0.0355 0.035 0.0047 0.0008 0.0151 0.0172 0.0316 0.0183 0.0027 0.0038 0.0003 0.0514
Lipid Particles 0.0205 0.0038 0.0053 0.0294 0.0071 0.0304 0.0166 0.0064 0.0012 0.0002 0.0361 0.0079 0.0743 0.0087 0.0019 0.0005 0.0007 0.0385
Mitochondria 0.8156 0.8721 0.9224 0.8664 0.919 0.931 0.8719 0.9102 0.9688 0.9973 0.8857 0.9614 0.507 0.874 0.9356 0.9753 0.9881 0.8881
None 0.0019 0.0001 0.0002 0.0001 0 0.0001 0.0031 0 0.0009 0 0.0005 0 0.003 0.0001 0.0001 0 0 0
Nuclear Periphery 0.0051 0.0006 0.0005 0.0001 0.0001 0.0006 0.0047 0 0.0023 0 0.0048 0 0.078 0 0 0 0 0.0001
Nucleolus 0.0005 0.0004 0.0001 0.0001 0.0001 0.0007 0.0004 0.0001 0.0001 0 0.0005 0 0.0026 0.0002 0.0001 0 0 0.0002
Nucleus 0.0009 0.0003 0.0001 0.0001 0.0001 0.0001 0.0016 0.0001 0.0006 0 0.0007 0 0.0068 0.0001 0 0 0 0.0001
Peroxisomes 0.0107 0.0172 0.0057 0.0083 0.0017 0.0031 0.0207 0.0096 0.0082 0.0006 0.0042 0.0054 0.0045 0.0484 0.0291 0.015 0.0101 0.005
Punctate Nuclear 0.0048 0.0001 0.0024 0.0001 0 0.0063 0.0014 0 0.0016 0 0.0023 0 0.0152 0.0001 0 0 0 0
Vacuole 0.004 0.0151 0.0008 0.0008 0.0027 0.0018 0.0018 0.0011 0.0004 0.0001 0.001 0.0003 0.016 0.001 0.0001 0.0001 0 0.0004
Vacuole Periphery 0.0062 0.0193 0.0019 0.0009 0.0077 0.008 0.0027 0.0029 0.0012 0.0003 0.0023 0.0023 0.0175 0.0009 0.0002 0.0003 0.0001 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.9596 40.5597 26.2548 35.2336 38.1747 30.3919 44.1728 49.4686 40.8083 43.3682
Translational Efficiency 0.6315 0.5262 0.7793 0.6025 0.4586 0.6914 0.5127 0.4692 0.581 0.4798

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Afg3

Afg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Afg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available