Standard name
Human Ortholog
Description Delta 1-pyrroline-5-carboxylate reductase; catalyzes the last step in proline biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0.05 0 0.05 0 0.08 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.96 0.96 0.94 0.86 0.8 0.86 0.79 0.85 0.79 0.98 1.0 1.0 0.98 0.97 0.96 0.98 0.95 0.95 0.74 0.83 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0.11 0.15 0.08 0.09 0.07 0.09 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 7 8 7 6 12 21 0 0 0 0 0 0 0 0 1 5 7 4
Bud 0 0 0 0 3 0 1 2 3 1 3 7 0 1 0 0 0 0 0 0 1 9 10 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 14 16 6 17 18 28 15 33 68 49 0 1 1 6 6 12 0 0 0 1 0 0
Cytoplasm 185 214 428 327 258 346 375 442 304 313 510 458 107 219 221 363 344 285 182 212 427 155 277 208
Endoplasmic Reticulum 1 1 7 6 3 14 47 81 27 35 43 52 2 0 0 13 16 9 0 0 4 7 5 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0
Golgi 0 0 2 0 1 0 2 3 1 2 1 6 0 0 0 1 2 2 0 0 1 0 0 0
Mitochondria 0 0 0 1 1 0 0 1 9 5 5 10 0 0 0 5 0 1 0 1 3 8 6 3
Nucleus 0 3 2 0 4 0 1 2 0 1 0 4 0 0 0 2 2 0 1 3 2 1 1 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 6 1 2 3 5 10 6 23 11 16 0 0 0 2 6 12 0 4 5 13 12 5
Unique Cell Count 186 218 438 340 270 368 435 550 353 394 602 580 109 219 221 369 355 297 187 225 450 210 334 250
Labelled Cell Count 188 219 459 351 278 381 456 577 372 419 653 623 109 221 222 392 376 321 187 225 450 210 334 250


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.2 19.2 26.4 26.4 22.7 27.7 26.3 28.4 24.5 25.2 26.6 24.2 27.7 27.1 26.8 24.1 30.0 32.3 22.0 24.6 24.3
Std Deviation (1e-4) 5.3 5.0 4.7 4.9 6.6 5.7 6.2 6.1 5.5 5.9 6.1 5.9 6.2 6.2 5.7 7.3 8.8 11.3 9.8 9.0 9.1
Intensity Change (Log2) – – – -0.0 -0.22 0.07 -0.01 0.1 -0.11 -0.07 0.01 -0.13 0.07 0.03 0.02 -0.13 0.18 0.29 -0.26 -0.1 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 3.0 4.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 -0.8 1.0 0.7 1.5 0.8 3.2 4.8 3.4 0 -2.2 -2.2 -1.4 -1.3 0.6
Cytoplasm -1.3 -1.6 -2.7 -6.3 -8.4 -6.2 -8.4 -7.0 -8.8 0.3 2.3 2.3 0.7 -0.7 -1.4
Endoplasmic Reticulum 0.2 0 2.0 5.6 7.2 4.2 4.8 4.1 5.0 0 0 0 1.8 2.4 1.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 -0.3 0.6 0.4 3.5 0.6 1.5 0 0 0 0 0.4 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.0633 28.2075 27.5857 26.4828 20.1745 26.6803 27.5227 33.6295 30.1226 29.9306 26.7681 29.9137 21.6178 27.5484 25.7175 24.6741 25.6306 24.5964
Actin 0.0083 0.0001 0.0023 0.0454 0.0015 0.0006 0.0099 0.0001 0.0063 0.0002 0.0137 0.0004 0.0099 0.0002 0.0009 0.0002 0.0001 0.0009
Bud 0.0004 0.0001 0.0002 0.0004 0.0035 0.0002 0.0003 0 0.0006 0.0001 0.0007 0.0001 0.0013 0.0001 0.0002 0.0003 0.0001 0.0002
Bud Neck 0.0008 0.0001 0.0003 0.0004 0.0007 0.0007 0.0002 0.0001 0.0003 0.0016 0.0007 0.0005 0.0004 0.0001 0.0003 0.0002 0.0004 0.0005
Bud Periphery 0.0008 0 0.0001 0.0004 0.0019 0.0001 0.0002 0 0.0011 0 0.0011 0 0.0004 0 0 0.0001 0 0.0001
Bud Site 0.0024 0.0001 0.002 0.0006 0.0022 0.0001 0.0004 0 0.0006 0 0.0024 0.0001 0.0054 0.0001 0.0013 0.0001 0.0001 0.0001
Cell Periphery 0.0014 0 0.0001 0.0001 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.9011 0.9977 0.9836 0.9107 0.7879 0.9622 0.9436 0.9976 0.9735 0.9857 0.8832 0.9749 0.9347 0.995 0.9835 0.9708 0.9896 0.95
Cytoplasmic Foci 0.0057 0.0004 0.0028 0.0043 0.0532 0.0026 0.0045 0.0004 0.0018 0.0027 0.0172 0.0031 0.0083 0.0008 0.0037 0.0008 0.0021 0.005
Eisosomes 0.0018 0 0.0001 0.0002 0.0001 0 0.0001 0 0 0 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0043 0.0003 0.0018 0.0012 0.0007 0.0028 0.0072 0.0005 0.0022 0.0033 0.0015 0.0021 0.0046 0.0005 0.0017 0.0034 0.0015 0.007
Endosome 0.0108 0.0001 0.0008 0.0086 0.0199 0.0027 0.0066 0.0001 0.0035 0.0035 0.0104 0.0027 0.0074 0.0004 0.0018 0.0026 0.0015 0.0145
Golgi 0.0031 0 0.0003 0.0088 0.0031 0.0005 0.0029 0 0.0011 0.0004 0.0091 0.0004 0.0015 0.0001 0.0002 0 0.0001 0.0014
Lipid Particles 0.0068 0 0.0017 0.0068 0.0064 0.0003 0.0034 0 0.0002 0 0.0143 0.0043 0.0019 0 0.0002 0 0 0.0012
Mitochondria 0.0199 0 0.0003 0.0026 0.0873 0.0217 0.0051 0 0.0015 0.0001 0.007 0.0005 0.0013 0 0.0001 0.0001 0 0.0011
None 0.0091 0.0001 0.0007 0.0008 0.0017 0.0002 0.0023 0.0001 0.0005 0.0001 0.0118 0.0003 0.0019 0.0002 0.0013 0.0002 0.0002 0.0005
Nuclear Periphery 0.0046 0.0003 0.0006 0.0011 0.0007 0.0009 0.0038 0.0003 0.0015 0.0004 0.0019 0.0011 0.0034 0.0005 0.0009 0.0066 0.0011 0.0103
Nucleolus 0.0009 0 0 0.0001 0.0013 0 0.0009 0 0 0 0.0003 0.0001 0.0002 0 0.0001 0 0 0
Nucleus 0.0059 0.0006 0.0011 0.0013 0.002 0.0021 0.0036 0.0006 0.0016 0.001 0.0015 0.0039 0.0053 0.0015 0.0017 0.0027 0.002 0.0035
Peroxisomes 0.0033 0 0.0004 0.0041 0.0052 0.0009 0.0011 0 0.0003 0 0.0148 0.0043 0.0017 0 0.0002 0 0 0.0001
Punctate Nuclear 0.0045 0 0.0004 0.0008 0.0006 0.0001 0.0009 0 0.0007 0 0.0067 0.0005 0.0051 0.0001 0.0008 0.0001 0.0001 0.0005
Vacuole 0.0033 0.0001 0.0004 0.0008 0.0153 0.0008 0.0019 0.0001 0.0019 0.0007 0.0009 0.0005 0.0043 0.0003 0.0008 0.0094 0.0011 0.0017
Vacuole Periphery 0.0011 0 0.0001 0.0005 0.0047 0.0003 0.0011 0 0.0006 0.0002 0.0005 0.0002 0.0006 0 0.0001 0.0023 0.0002 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 231.3177 238.2883 255.8793 239.7684 259.205 242.0766 241.5122 281.4466 295.458 249.2304
Translational Efficiency 1.4283 1.4938 1.437 1.8223 1.46 1.3133 1.6399 1.4571 1.4271 1.4361

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Delta 1-pyrroline-5-carboxylate reductase; catalyzes the last step in proline biosynthesis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Pro3

Pro3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pro3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available