Standard name
Human Ortholog
Description Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.31 0.33 0.31 0.29 0.42 0.34 0.26 0.21 0.21 0.2 0.22 0.17 0.23 0.28 0.48 0.45 0.49 0.43 0.24 0.18 0.16 0.37 0.57 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 0.15 0.32 0.43 0.41 0.7 0.78 0.71 0.78 0.79 0.8 0.85 0 0 0 0.06 0.07 0 0.09 0 0.09 0 0 0
Nucleus 0.15 0.36 0.31 0.28 0.2 0.16 0.19 0.27 0.23 0.24 0.2 0.2 0.72 0.66 0.36 0.27 0.2 0.17 0.27 0.44 0.31 0.2 0.08 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.09 0.1 0.09 0.07
Vac/Vac Membrane 0.08 0.26 0.1 0.09 0.09 0.06 0.06 0.06 0 0 0.06 0.07 0 0.08 0.15 0.21 0.21 0.35 0.21 0.18 0.18 0.18 0.11 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 1 3
Bud 1 1 3 4 10 14 11 17 11 17 17 9 0 0 0 1 0 0 3 7 7 6 5 5
Bud Neck 0 0 1 0 0 1 0 0 0 0 1 0 3 1 1 1 0 1 1 2 2 3 4 6
Bud Site 0 0 2 1 2 13 8 11 17 29 26 15 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 0 0 0 0 0 0 0 0
Cytoplasm 50 52 106 75 146 186 165 115 106 115 113 96 78 102 212 154 142 121 49 37 76 106 168 280
Endoplasmic Reticulum 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 24 18 16 0 0 4 1 1 1
Endosome 0 2 25 8 15 0 3 6 1 0 0 0 5 6 23 20 18 23 5 3 15 6 7 12
Golgi 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 1 0 0 0
Mitochondria 96 24 108 109 143 377 500 389 401 457 410 472 1 0 0 20 20 11 17 5 45 9 11 11
Nucleus 24 57 104 72 71 85 120 148 118 140 100 113 250 245 156 91 59 48 54 91 150 57 23 10
Nuclear Periphery 0 0 0 0 0 1 1 3 0 1 1 6 0 0 0 0 1 0 0 0 3 0 0 0
Nucleolus 0 0 0 0 1 1 3 3 4 10 2 3 0 1 0 1 1 0 1 4 19 4 2 1
Peroxisomes 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 11 16 2 2 4 8 9 4 4 2 5 9 8 5 10 4 16 11 45 30 27 26
Vac/Vac Membrane 13 41 33 24 32 32 38 35 21 22 31 39 14 30 66 72 62 99 42 36 88 51 32 20
Unique Cell Count 160 159 338 255 348 541 643 550 511 579 511 555 345 369 439 339 291 284 204 208 492 290 298 397
Labelled Cell Count 185 178 393 310 423 713 854 735 688 797 705 756 357 395 467 393 332 323 204 208 492 290 298 397


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.4 3.9 3.8 3.8 3.2 3.1 3.3 3.0 3.1 2.6 2.8 5.6 5.8 5.4 6.0 5.4 5.6 5.4 5.6 5.4
Std Deviation (1e-4) 0.5 0.7 1.0 1.2 1.5 1.3 1.1 0.9 0.9 0.8 0.8 0.5 0.9 1.0 0.9 1.5 1.4 1.3 1.2 1.7 1.4
Intensity Change (Log2) – – – -0.04 -0.03 -0.26 -0.31 -0.25 -0.36 -0.35 -0.56 -0.46 0.54 0.57 0.46 0.63 0.48 0.53 0.47 0.53 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.1 4.0 7.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7293 2.413 1.3183 0.8554 1.5197 1.3333 1.0049 0.1419 0.251 0.7255 0.2358 0.3551 -0.211 -0.1852 0.0558 0.7203 -0.2576 -0.0745
Actin 0.0481 0.0005 0.0025 0.0005 0.0004 0.0056 0.0486 0.0023 0.0054 0.0477 0.0212 0.0048 0.0097 0.0003 0.0093 0.0091 0.0001 0.0013
Bud 0.0097 0.0074 0.0055 0.0003 0.0001 0.0023 0.0011 0.0007 0.0004 0.0083 0.0087 0.0005 0.0003 0.0002 0.0002 0.0009 0 0.0001
Bud Neck 0.0064 0.001 0.0016 0.0019 0.0114 0.0182 0.011 0.0008 0.0011 0.0016 0.0012 0.0148 0.0017 0.0007 0.0007 0.004 0.0005 0.0025
Bud Periphery 0.0027 0.0011 0.0008 0.0004 0.0001 0.0022 0.0019 0.0007 0.0005 0.0186 0.0437 0.0008 0.0005 0.0002 0.0002 0.0023 0 0.0002
Bud Site 0.0444 0.009 0.0042 0.0016 0.0002 0.0018 0.017 0.0075 0.0024 0.0099 0.0022 0.0014 0.0036 0.0008 0.0011 0.0023 0.0001 0.0001
Cell Periphery 0.0007 0.0003 0.0001 0 0 0.0001 0.0006 0.0001 0.0001 0.0008 0.0006 0.0001 0.0003 0 0.0001 0.0003 0 0
Cytoplasm 0.0777 0.1448 0.0564 0.067 0.0621 0.0651 0.0896 0.1523 0.0826 0.1386 0.0745 0.0974 0.0871 0.091 0.0681 0.0614 0.0264 0.0554
Cytoplasmic Foci 0.0412 0.0153 0.0253 0.0101 0.0189 0.0039 0.0202 0.0049 0.005 0.006 0.004 0.0081 0.0203 0.0018 0.0103 0.0128 0.0075 0.0025
Eisosomes 0.0004 0.0001 0.0001 0 0.0001 0.0001 0.0006 0 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0127 0.005 0.0025 0.0012 0.0016 0.0011 0.006 0.003 0.0015 0.0039 0.002 0.0033 0.0048 0.0028 0.0027 0.0051 0.0007 0.0008
Endosome 0.057 0.0059 0.0065 0.0018 0.0543 0.0032 0.0248 0.0034 0.0042 0.0382 0.0034 0.0087 0.0118 0.0019 0.0072 0.0144 0.0014 0.0023
Golgi 0.0203 0.0006 0.0032 0.0007 0.001 0.0009 0.0086 0.0002 0.0007 0.0074 0.0005 0.0016 0.0021 0.0001 0.0022 0.0027 0.0005 0.0008
Lipid Particles 0.0254 0.0023 0.0161 0.001 0.0044 0.0013 0.0077 0.0003 0.0006 0.0012 0.0002 0.0052 0.0076 0.0001 0.0044 0.0019 0.0043 0.001
Mitochondria 0.0114 0.001 0.0017 0.0007 0.0013 0.0032 0.0168 0.0008 0.0139 0.0109 0.0015 0.0026 0.0031 0.0005 0.0053 0.0673 0.0001 0.0035
None 0.1209 0.2891 0.1919 0.172 0.2092 0.1057 0.1776 0.1938 0.1374 0.1612 0.1928 0.0502 0.1178 0.0934 0.0877 0.152 0.0468 0.0755
Nuclear Periphery 0.0356 0.0222 0.0226 0.0107 0.0152 0.0082 0.0409 0.0149 0.0104 0.0099 0.0062 0.0184 0.0218 0.0117 0.0143 0.0174 0.0087 0.0062
Nucleolus 0.009 0.0085 0.007 0.0083 0.0117 0.0137 0.0082 0.0052 0.0066 0.003 0.0101 0.0106 0.0076 0.0062 0.0067 0.0151 0.009 0.0106
Nucleus 0.3638 0.4153 0.566 0.6154 0.5421 0.6989 0.4544 0.5604 0.6854 0.449 0.5895 0.7016 0.631 0.7606 0.6885 0.576 0.7757 0.788
Peroxisomes 0.0106 0.0021 0.0115 0.0051 0.0007 0.0009 0.0055 0.0012 0.0009 0.0014 0.0001 0.0053 0.0069 0 0.0037 0.0051 0.0302 0.0003
Punctate Nuclear 0.0946 0.0645 0.0734 0.1003 0.0593 0.0627 0.0522 0.0437 0.0378 0.0694 0.0357 0.0618 0.0573 0.026 0.0855 0.0454 0.0876 0.0479
Vacuole 0.0049 0.0034 0.0008 0.0009 0.0049 0.0008 0.0044 0.0032 0.0027 0.0104 0.0015 0.0021 0.0035 0.0013 0.0014 0.0032 0.0004 0.0007
Vacuole Periphery 0.0024 0.0007 0.0003 0.0002 0.0011 0.0004 0.0023 0.0005 0.0005 0.0025 0.0004 0.0009 0.001 0.0003 0.0004 0.001 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.777 8.3238 8.6473 6.5669 9.0545 7.445 9.6787 10.2247 8.0058 9.8018
Translational Efficiency 0.7859 0.9676 0.9185 0.6499 0.849 1.0391 0.7663 0.7878 0.781 0.9452

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes
Localization
Cell Percentages nucleus (71%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mig3

Mig3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mig3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available