Standard name
Human Ortholog
Description Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0 0.05 0 0 0 0 0 – 0 0 0.07 0 0 0.13 0.11 0.09 0.05 0.07 0.06 0.07 0.06 0.05
Bud 0 0 0 0 0 0 0 0 0 – 0.05 0 0.08 0.05 0.08 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0 0 0.05 – 0 0.05 0 0 0 0 0 0 0.07 0.06 0.07 0.09 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 – 0 0 0 0.1 0.07 0.09 0.11 0.06 0 0 0 0 0 0
Cytoplasm 0.95 0.87 0.93 0.91 0.93 0.92 0.84 0.91 0.82 – 0.74 0.8 0.77 0.78 0.75 0.75 0.77 0.77 0.72 0.69 0.64 0.57 0.58 0.61
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0.06 0.06
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0.1 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.15 0.06 0.19 – 0.39 0.24 0 0 0 0 0 0 0 0.06 0.13 0.13 0.1 0.1
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0.1 0.13 0.14 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0.07 0.08 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 19 18 14 8 0 11 0 6 – 0 1 23 17 14 38 48 29 13 21 23 9 30 18
Bud 4 10 5 10 3 3 13 3 11 – 19 6 26 22 43 8 7 7 6 7 5 3 20 6
Bud Neck 2 0 28 10 10 2 19 12 17 – 5 17 0 0 1 0 4 1 20 17 28 11 19 14
Bud Site 0 1 3 0 3 1 4 0 1 – 1 4 5 0 2 1 0 3 – – – – – –
Cell Periphery 7 9 15 15 10 4 6 4 5 – 0 9 10 47 36 26 46 19 2 5 9 1 10 8
Cytoplasm 259 252 385 253 331 328 461 353 287 – 284 253 243 357 389 219 336 257 208 222 272 71 282 234
Endoplasmic Reticulum 0 15 3 1 3 1 5 0 3 – 0 1 1 1 3 20 20 16 2 6 4 2 31 21
Endosome 1 0 1 0 0 2 0 1 0 – 0 0 17 26 52 6 9 12 9 10 5 1 3 4
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 4 4 2 2 1 1 0 2 1
Mitochondria 6 2 0 6 6 37 82 24 66 – 148 76 5 3 1 3 4 7 11 18 56 15 46 38
Nucleus 1 0 1 1 4 3 3 4 1 – 1 0 1 1 1 1 5 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 0 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 2
Peroxisomes 0 1 0 0 0 0 1 0 0 – 0 0 32 61 73 1 3 3 0 0 0 0 2 2
SpindlePole 3 1 11 8 11 5 9 9 5 – 8 7 14 28 27 20 33 24 1 0 3 0 3 2
Vac/Vac Membrane 1 1 2 0 1 0 4 1 0 – 2 0 1 0 1 8 20 9 3 8 6 5 25 19
Unique Cell Count 274 289 413 279 357 357 551 389 350 383 318 316 460 518 291 437 332 289 324 426 126 485 385
Labelled Cell Count 292 312 472 318 390 387 618 411 402 468 374 378 563 644 355 540 389 289 324 426 126 485 385


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.8 5.5 5.2 5.4 4.2 4.2 4.6 3.5 – 3.3 3.8 5.5 5.8 5.8 8.5 8.8 7.8 5.4 5.4 5.8
Std Deviation (1e-4) 0.8 0.6 0.9 1.4 1.2 0.9 0.8 1.4 0.6 – 0.5 0.8 1.3 1.8 1.7 1.6 1.8 1.5 2.2 2.2 2.1
Intensity Change (Log2) – – – -0.1 -0.03 -0.4 -0.41 -0.27 -0.66 – -0.76 -0.54 -0.01 0.07 0.08 0.61 0.67 0.5 -0.05 -0.05 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 -1.6 0 0 0 0 – 0 0 1.7 -0.5 -1.4 4.2 3.6 2.4
Bud 2.1 0 0 0 0 0 – 0 0 4.7 3.0 4.9 1.5 0.5 1.0
Bud Neck -1.8 -2.5 0 0 0 0 – 0 0 -4.7 -5.7 -5.7 -4.5 -4.5 -4.5
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 1.1 -0.6 0 0 0 0 – 0 0 -0.3 3.8 2.2 3.0 3.9 1.4
Cytoplasm -1.2 -0.3 -0.7 -4.5 -1.3 -4.8 – -7.3 -5.5 -6.3 -6.4 -7.3 -6.7 -6.6 -6.2
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 4.5 3.5 3.5
Endosome 0 0 0 0 0 0 – 0 0 4.4 4.6 6.4 0 0 3.5
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 6.6 7.7 7.9 0 0 0
SpindlePole 0.2 0.3 0 0 0 0 – 0 0 1.3 2.4 2.0 2.7 3.2 2.9
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 3.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2366 2.9952 2.6172 2.2309 2.1602 2.7621 1.6018 1.4489 1.6444 1.5547 1.3994 1.5176 1.6025 1.5297 1.3218 1.6584 1.8312 1.5403
Actin 0.1114 0.0834 0.152 0.1312 0.1 0.0602 0.1109 0.059 0.1166 0.1205 0.2308 0.0751 0.0643 0.0069 0.0967 0.1014 0.1339 0.0366
Bud 0.0035 0.0073 0.0194 0.028 0.0243 0.0009 0.0138 0.0258 0.0472 0.0452 0.0407 0.0051 0.0226 0.0506 0.0689 0.0696 0.0214 0.0448
Bud Neck 0.0125 0.0062 0.0097 0.1306 0.0125 0.2068 0.0144 0.0062 0.0108 0.0454 0.0401 0.2334 0.011 0.0038 0.0047 0.0051 0.0623 0.1813
Bud Periphery 0.0146 0.0089 0.0897 0.1731 0.144 0.003 0.0265 0.0242 0.1288 0.1228 0.1118 0.0138 0.0348 0.0222 0.1384 0.1659 0.0408 0.0431
Bud Site 0.0494 0.1923 0.0345 0.0046 0.0084 0.0021 0.071 0.1406 0.0796 0.0154 0.0217 0.0104 0.0714 0.1886 0.0586 0.0206 0.0397 0.0098
Cell Periphery 0.0164 0.0113 0.0116 0.0125 0.0096 0.0041 0.0088 0.0032 0.0049 0.0042 0.0071 0.0033 0.0089 0.0047 0.0048 0.0103 0.0047 0.0036
Cytoplasm 0.4012 0.4174 0.4219 0.3495 0.3374 0.4415 0.4921 0.505 0.3965 0.3987 0.3461 0.5084 0.4228 0.4113 0.279 0.2668 0.3102 0.3972
Cytoplasmic Foci 0.0679 0.0354 0.0229 0.0343 0.0713 0.047 0.0602 0.0358 0.0332 0.0358 0.0262 0.0422 0.0721 0.0243 0.0323 0.0586 0.0372 0.0247
Eisosomes 0.0029 0.0016 0.0018 0.0011 0.0014 0.0009 0.0009 0.0004 0.001 0.0009 0.0013 0.0004 0.0012 0.0006 0.0005 0.0005 0.0014 0.0003
Endoplasmic Reticulum 0.0061 0.0037 0.0078 0.0044 0.0034 0.0029 0.0038 0.0028 0.004 0.0124 0.0026 0.0019 0.0102 0.0029 0.0027 0.0049 0.012 0.0022
Endosome 0.0121 0.0061 0.0093 0.0162 0.0118 0.0048 0.0237 0.0077 0.0203 0.0322 0.0135 0.0068 0.0189 0.0092 0.0099 0.0195 0.0206 0.0025
Golgi 0.0111 0.0082 0.0068 0.0103 0.0059 0.0032 0.0112 0.0052 0.0083 0.0088 0.0138 0.0047 0.0065 0.0017 0.006 0.0114 0.014 0.0017
Lipid Particles 0.008 0.0022 0.0036 0.0019 0.0261 0.0038 0.0085 0.0012 0.0032 0.0095 0.0045 0.0005 0.0096 0.0006 0.0037 0.0143 0.0044 0.0005
Mitochondria 0.0031 0.0007 0.0047 0.0148 0.0122 0.0018 0.011 0.0042 0.0079 0.0123 0.0054 0.0048 0.0191 0.0017 0.0064 0.0113 0.0132 0.002
None 0.2643 0.2035 0.1772 0.0807 0.2044 0.1997 0.102 0.1607 0.1155 0.0951 0.0644 0.0774 0.1889 0.2446 0.2313 0.1358 0.2273 0.2172
Nuclear Periphery 0.0008 0.0004 0.0017 0.0004 0.0008 0.0008 0.0046 0.0006 0.0023 0.0097 0.0022 0.0003 0.0046 0.0014 0.0036 0.0038 0.0181 0.0031
Nucleolus 0.0008 0.0004 0.0007 0.0002 0.0016 0.0002 0.0034 0.0014 0.0009 0.0011 0.0178 0.0003 0.0032 0.0025 0.003 0.0044 0.0041 0.0009
Nucleus 0.0011 0.0019 0.0014 0.0006 0.0011 0.0005 0.0052 0.0031 0.0034 0.0067 0.0141 0.0012 0.0044 0.0099 0.0195 0.0105 0.0065 0.0134
Peroxisomes 0.0071 0.0061 0.0112 0.0018 0.0113 0.0125 0.0184 0.0083 0.0062 0.0041 0.0279 0.0063 0.0113 0.0015 0.0135 0.0632 0.0118 0.0031
Punctate Nuclear 0.0025 0.0006 0.0075 0.0005 0.0094 0.0021 0.0025 0.0015 0.0043 0.006 0.0042 0.0024 0.0073 0.0077 0.0115 0.0103 0.0108 0.0087
Vacuole 0.0031 0.0022 0.0038 0.0026 0.0021 0.001 0.0059 0.0027 0.0041 0.0109 0.0033 0.0012 0.0056 0.0029 0.0041 0.0102 0.0043 0.0028
Vacuole Periphery 0.0004 0.0001 0.0007 0.0006 0.0009 0.0002 0.0013 0.0004 0.001 0.0023 0.0007 0.0002 0.0011 0.0004 0.0009 0.0017 0.0016 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.5293 5.1259 6.5206 8.7761 4.2483 6.959 8.5146 10.869 11.4775 9.4069
Translational Efficiency 1.6321 1.2111 1.2309 0.8979 1.542 1.314 1.1263 0.8768 0.8027 1.0284

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system
Localization
Cell Percentages cytoplasm (81%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sap1

Sap1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sap1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available