Standard name
Human Ortholog
Description Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.05 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0.05 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.97 0.99 0.95 0.95 0.82 0.77 0.81 0.75 0.75 0.76 0.99 0.98 0.96 0.98 0.99 0.99 0.99 0.95 0.92 0.93 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.14 0.17 0.09 0.15 0.15 0.05 0 0 0 0.06 0.08 0.07 0 0 0.05 0 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 8 7 12 16 4 12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3 2 2 0 0 2 0 1 0 1 0 0 0 2 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 2 5 5 20 10 12 19 5 6 0 0 2 3 6 3 0 0 0 0 0 0
Cytoplasm 128 193 299 182 294 234 328 303 210 241 76 87 94 197 238 717 384 303 120 184 276 82 204 140
Endoplasmic Reticulum 0 3 9 0 11 9 55 69 22 50 15 6 1 4 11 45 31 21 0 2 16 3 9 10
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 1 0 0 3 7 6 7 3 2 0 0 0 3 1 0 0 0 0 0 1 0
Mitochondria 0 0 0 0 0 0 2 3 13 19 5 8 0 0 0 5 1 0 0 0 1 0 1 1
Nucleus 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 0 1 6 2 0 2 2 0 0 0 2 1 0 0 1 1 0 0 2
Unique Cell Count 128 196 308 184 309 246 402 395 258 323 102 115 95 200 248 728 388 306 122 195 302 89 220 158
Labelled Cell Count 128 196 311 186 314 249 421 409 280 352 111 126 95 202 251 776 424 327 122 195 302 89 220 158


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 89.2 101.5 86.0 85.3 72.5 64.2 51.4 43.8 41.6 38.4 33.8 36.7 91.1 88.6 86.2 115.0 133.8 142.1 80.3 84.3 86.6
Std Deviation (1e-4) 19.5 24.0 20.6 23.6 20.5 15.9 13.2 12.9 13.5 11.9 10.8 13.4 16.6 18.5 17.1 28.6 35.8 41.1 23.6 17.7 19.1
Intensity Change (Log2) – – – -0.01 -0.25 -0.42 -0.74 -0.97 -1.05 -1.16 -1.35 -1.23 0.08 0.04 0.0 0.42 0.64 0.73 -0.1 -0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.8 4.0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.3 1.9 3.1 3.7 0 0 0 0 0 0 0 0
Cytoplasm 1.3 -1.2 -1.2 -6.4 -7.6 -6.2 -8.0 -7.1 -6.9 1.0 1.0 -0.7 1.5 1.8 1.7
Endoplasmic Reticulum 0 0.4 0.5 5.0 6.1 2.9 5.4 4.4 0 0 -0.6 1.0 2.2 2.9 2.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.0 4.3 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 85.059 117.4392 104.1007 104.882 89.5673 115.8765 84.2606 122.0421 107.0797 94.5135 107.7273 114.6937 90.1508 127.4035 109.1946 113.357 87.742 110.7711
Actin 0.0028 0.0006 0.0024 0.0001 0.0026 0.0005 0.0141 0.0005 0.0083 0.014 0.0002 0.0048 0.0119 0.0003 0.002 0.0001 0.0347 0.0028
Bud 0.0024 0.0009 0.0017 0.0016 0.0011 0.0009 0.0022 0.0006 0.0017 0.0025 0.0005 0.0073 0.0015 0.0003 0.0013 0.0015 0.0016 0.0024
Bud Neck 0.001 0.0009 0.0011 0.0011 0.0009 0.0017 0.0009 0.0003 0.0005 0.0011 0.0006 0.0025 0.0007 0.0003 0.0006 0.0006 0.0006 0.0018
Bud Periphery 0.0041 0.0011 0.0019 0.0016 0.0005 0.0006 0.003 0.0003 0.0012 0.0034 0.0001 0.0051 0.0018 0.0001 0.0012 0.0007 0.0025 0.0019
Bud Site 0.0012 0.0008 0.0007 0.0003 0.0002 0.0002 0.0032 0.0011 0.0035 0.0004 0 0.0029 0.0017 0.0003 0.0007 0.0003 0.0014 0.0022
Cell Periphery 0.0312 0.018 0.0239 0.0305 0.0032 0.0035 0.0087 0.0036 0.0091 0.0062 0.0005 0.003 0.0127 0.0124 0.0102 0.0164 0.0031 0.0303
Cytoplasm 0.5924 0.8324 0.6933 0.4857 0.7095 0.8081 0.8388 0.9573 0.9024 0.7594 0.9644 0.7686 0.7421 0.9558 0.8429 0.7721 0.6614 0.8158
Cytoplasmic Foci 0.0041 0.0007 0.0018 0.001 0.0046 0.0026 0.0099 0.0041 0.0023 0.0019 0.002 0.0128 0.0142 0.0006 0.0115 0.0029 0.0212 0.0161
Eisosomes 0.0002 0.0005 0.0004 0 0.0001 0 0.0018 0 0 0.0002 0 0 0.0004 0 0.0002 0 0.0004 0.0006
Endoplasmic Reticulum 0.0493 0.0267 0.017 0.0177 0.0082 0.0089 0.0283 0.0062 0.0047 0.0085 0.0037 0.0188 0.0374 0.0024 0.0101 0.0018 0.0085 0.0231
Endosome 0.0083 0.0018 0.005 0.0055 0.0111 0.0284 0.0073 0.0009 0.008 0.0134 0.0036 0.0312 0.0108 0.0007 0.0077 0.0083 0.0105 0.0063
Golgi 0.0015 0.0006 0.0008 0.0001 0.0043 0.0026 0.0032 0.0004 0.0007 0.0051 0.0001 0.0086 0.0044 0.0001 0.0055 0.0003 0.0056 0.0017
Lipid Particles 0.0044 0.0011 0.0017 0.0005 0.0342 0.0006 0.0039 0.0008 0.0005 0.0008 0.0002 0.0018 0.01 0.0006 0.0041 0.0018 0.0083 0.0062
Mitochondria 0.0094 0.0007 0.0068 0.0004 0.009 0.0023 0.0101 0.0015 0.0006 0.0608 0.0001 0.0609 0.0143 0.0001 0.0075 0.0005 0.0641 0.0032
None 0.0028 0.0002 0.0006 0.0002 0.0008 0.0003 0.006 0.0003 0.0035 0.0006 0.0003 0.0016 0.0046 0.0001 0.0043 0.0004 0.0138 0.0102
Nuclear Periphery 0.0443 0.0085 0.0112 0.0169 0.0272 0.0109 0.0091 0.0009 0.0047 0.0092 0.0051 0.0026 0.0203 0.0024 0.0064 0.0038 0.0074 0.0037
Nucleolus 0.0018 0.0003 0.0009 0.0007 0.0007 0.0002 0.0008 0.0002 0.0002 0.0002 0 0.0014 0.0018 0.0001 0.0007 0.0006 0.0022 0.0073
Nucleus 0.012 0.0027 0.005 0.0065 0.004 0.0041 0.005 0.0009 0.0027 0.0035 0.0026 0.0055 0.0054 0.0011 0.0026 0.0037 0.0039 0.0078
Peroxisomes 0.0017 0 0.0035 0 0.0099 0.0001 0.0005 0.0003 0.0001 0.0001 0 0.0008 0.0025 0 0.0008 0.0001 0.0049 0.0004
Punctate Nuclear 0.0088 0 0.0001 0 0.0016 0.0001 0.0011 0.0001 0.0006 0.0001 0.0001 0.0002 0.0013 0 0.0006 0 0.0009 0.0068
Vacuole 0.1594 0.0866 0.178 0.3374 0.1135 0.0759 0.0371 0.0184 0.0396 0.0856 0.0128 0.0444 0.0814 0.021 0.0682 0.1577 0.077 0.0414
Vacuole Periphery 0.057 0.015 0.0421 0.092 0.0527 0.0476 0.0049 0.0012 0.0051 0.023 0.0028 0.015 0.019 0.0013 0.011 0.0264 0.0662 0.0079

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 91.9504 71.6119 70.6981 108.3308 104.3155 93.9881 92.0825 104.5386 91.6126 108.77
Translational Efficiency 1.3987 1.7981 1.6568 1.1541 1.316 1.3581 1.4304 1.161 1.3864 1.2402

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Hom3

Hom3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hom3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available